Chin J Plan Ecolo ›› 2012, Vol. 36 ›› Issue (12): 1256-1267.doi: 10.3724/SP.J.1258.2012.01256


• Research Articles • Previous Articles     Next Articles

Intra-annual variation in δ13C from tree rings of Pinus sylvestris var. mongolica and its response to climatic factors

SHANG Zhi-Yuan1*, WANG Jian1, CUI Ming-Xing2, and CHEN Zhen-Ju3   

  1. 1College of Geographical Science, Nanjing Normal University, Nanjing 210023, China;

    2Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China;

    3Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2012-05-09 Revised:2012-10-19 Online:2012-11-28 Published:2012-12-01
  • Contact: SHANG Zhi-Yuan


Aims We assess the relationship among the carbon isotopic signatures of earlywood (EW), transitional wood (TW) and latewood (LW) from tree rings. Our aims were to investigate variation in the intra-annual stable carbon isotope ratio (δ13C) in Pinus sylvestris var. mongolica and determine the relationship between them and homologous ring width.
Methods Based on two tree discs of Pinus sylvestris var. mongolica sampled from the northern part of Daxing’an Mountains in China, the EW, TW and LW were obtained with different stripping and pooling programs. After performing ring widths measurement and cross-dating, the periods analyzed were the maximum growth periods for one sample and different growth periods for the other. The holocellulose fractions were extracted and the intra-annual δ13C of samples were measured.
Important findings In general, the δ13C values of TW are the highest, EW come second and LW are the lowest. The intra-annual trend of δ13C is fluctuateing prominently from the juvenile period to the fast-growing period and is smoother from the maturation period to the senescence period. The variation amplitude of LW is almost greater than EW at the same period. The δ13C of LW is always prominently higher than EW for the juvenile period. The difference between EW and LW is indistinctive for the maturation period and is negligible for the senescence period. The intra-annual variability of δ13C concentrates on the middle and later phase of the growing season. The correlation relationship between the intra-annual δ13C sequences and homologous detrended ring width sequences (dRWS) decreases with the seasons, which implies that environmental factors play a dominant role in cell formation and carbon fractionation during the middle and later phase of the growing season in each year. The ring width of EW of the current year is positively correlated with LW of the previous year (pLW). Also the δ13C of EW is negative correlated with the incorporative dRWS of EW + pLW. But the correlation between δ13C of EW and δ13C or dRWS of pLW is statistically insignificant. The growing season could be divided as: EW (from late April to middle June, with greater soil moisture and rapidly increasing temperature), TW (from late June to middle July, with lower soil moisture and maximum temperature) and LW (from late July to middle September, with greater soil moisture and decreased temperature).

[1] Barbour MM, Walcroft AS, Farquhar GD (2002). Seasonal variation in δ13C and δ18O of cellulose from growth rings of Pinus radiata. Plant, Cell and Environment, 25(11), 1483-1499. CrossRef
[2] Brugnoli E, Hubick KT, Caemmerer SV, Wong SC, Farquhar GD (1988). Correlation between the carbon isotope discrimination in leaf starch and sugars of C3-plants and the ratio of intercellular and atmospheric partial pressures of carbon dioxide. Plant Physiology, 88, 1418-1424. CrossRef
[3] Damesin C, Lelarge C (2003). Carbon isotope composition of current-year shoots from Fagus sylvatica in relation to growth, respiration and use of reserves. Plant, Cell and Environment, 26, 207-219. CrossRef
[4] Eglin T, Maunoury-Danger F, Fresneau C, Lelarge C, Pollet B, Lapierre C, Francois C, Damesin C (2008). Biochemical composition is not the main factor influencing variability in carbon isotope composition of tree rings. Tree Physiology, 28, 1619-1628. CrossRef
[5] Eilmann B, Buchmann N, Siegwolf R, Saurer M, Cherubini P, Rigling A (2010). Fast response of Scots pine to improved water availability reflected in tree-ring width and δ13C. Plant, Cell and Environment, 33(8), 1351-1360. CrossRef
[6] Francey RJ, Farquhar GD (1982). An explanation of 13C/12C variations in tree rings. Nature, 297(5861), 28-31. CrossRef
[7] Helle G, Schleser GH (2004). Beyond CO2-fixation by Rubisco-an interpretation of 13C/12C variations in tree rings from novel intra-seasonal studies on broad-leaf trees. Plant, Cell and Environment, 27, 367-380. CrossRef
[8] Hill SA, Waterhouse JS, Field EM, Switsur VR, Aprees T (1995). Rapid recycling of triose phosphates in oak stem tissue. Plant, Cell and Environment, 18(8), 931-936. 
[9] Hoch G., Richter A & Korner C (2003). Non-structural carbon compounds in temperate forest trees. Plant, Cell and Environment, 26, 1067-1081. CrossRef
[10] J?ggi M, Saurer M, Fuhrer J, Siegwolf R (2002). The relationship between the stable carbon isotope composition of needle bulk material, starch, and tree rings in Picea abies. Oecologia, 131, 325-332. CrossRef
[11] Kagawa A, Sugimoto A, Maximov TC (2006). 13CO2 pulse-labelling of photoassimilates reveals carbon allocation within and between tree rings. Plant, Cell and Environment, 29, 1571-1584. CrossRef
[12] Kress A, Young GHF, Saurer M, Loader NJ, Siegwolf RTW, McCarroll D (2009). Stable isotope coherence in the earlywood and latewood of tree-line conifers. Chemical Geology, 268, 52-57. CrossRef
[13] Lacointe A, Kajji A, Daudet FA, Archer P, Frossard JS (1993). Mobilization of carbon reserves in young walnut trees. Acta Botanica Gallica, 140, 435-441. CrossRef
[14] Leavitt SW (1993). Seasonal 13C/12C changes in tree rings: species and site coherence, and a possible drought influence. Canadian Journal Forest Research, 23(2), 210-218. CrossRef
[15] Leavitt SW, Long A (1991). Seasonal stable-carbon isotope variability in tree rings: possible palaeoenvironmental signals. Chemical Geology, 87(1), 59-70. CrossRef
[16] Leavitt SW, Wright WE, Long A (2002). Spatial expression of ENSO, drought, and summer monsoon in seasonal δ13C of ponderosa pine tree rings in southern Arizona and New Mexico. Journal Geophysical Research, 107(D18), 4349. 
[17] Li ZH, Leavitt SW, Mora CI, Liu RM (2005). Influence of earlywood-latewood size and isotope differences on long-term tree-ring δ13C trends, Chemical Geology, 216, 191-201. CrossRef
[18] Livingston NJ, Spittlehouse DL (1996). Carbon isotope fractionation in tree ring early and late wood in relation to intra-growing season water balance. Plant, Cell and Environment, 19, 768-774. CrossRef
[19] Loader NJ, Switsur VR, Field EM (1995). High-resolution stable isotope analysis of tree rings: implications of ‘microdendroclimatology’ for palaeoenvironmental research. The Holocene, 5(4), 457-460. 
[20] McCarroll D, Jalkanen R, Hicks S, Tuovinen M, Gagen M, Pawellek F, Eckstein D, Schmitt U, Autio J, Heikkinen O (2003). Multiproxy dendroclimatology: a pilot study in northern Finland. The Holocene, 13(6), 829-838. CrossRef
[21] McCarroll D, Loader NJ (2004). Stable isotope is tree rings. Quaternary Science Reviews, 23, 771-801. CrossRef
[22] McCarroll D, Pawellek F (2001). Stable carbon isotope ratios of Pinus sylvestris from northern Finland and the potential for extracting a climate signal from long Fennoscandian chronologies. The Holocene, 11, 517-526. CrossRef
[23] Michelot A, Eglin T, Dufrêne E, Lelarge-Trouverie C, Damesin C (2011). Comparison of seasonal variations in water-use efficiency calculated from the carbon isotope composition of tree rings and flux data in a temperate forest. Plant, Cell and Environment, 34(2), 230-244. CrossRef
[24] O’Leary MH (1981). Carbon isotope fractionation in plants. Phytochemistry, 20, 553-567. CrossRef
[25] Porté A, Loustau D (2001). Seasonal and interannual variations in carbon isotope discrimination in a maritime pine (Pinus pinaster) stand assessed from the isotopic composition of cellulose in annual rings. Tree Physiology, 21, 861-868. CrossRef
[26] Robertson I, Loader NJ, McCarroll D, Carter AHC, Cheng L, Leavitt SW (2004). δ13C of tree-ring lignin as an indirect measure of climate change. Water, Air, and Soil Pollution, 4, 531-544. CrossRef
[27] Schulze B, Wirth C, Linke P, Brand WA, Kuhlmann I, Horna V, Schulze ED (2004). Laser ablation-combustion-GC-IRMS - a new method for online analysis of intra-annual variation of δ13C in tree rings. Tree Physiology, 24, 1193-1201. CrossRef
[28] Shang ZY (商志远), Wang J (王建), Cui MX (崔明星), Chen ZJ (陈振举), Wang ZJ (王志军), Liu F (刘丰), Qian JL (钱君龙) (2011). Analysis of stable carbon isotopes in different components of tree rings of Pinus sylvestris var. mongolica. Acta Ecologica Sinica (生态学报), 31(18), 5148-5158. CrossRef
[29] Skomarkova MV, Vaganov EA, Mund M, Knohl A, Linke P, Boerner A, Schulze ED (2006). Inter-annual and seasonal variability of radial growth, wood density and carbon isotope ratios in tree rings of beech (Fagus sylvatica) growing in Germany and Italy. Trees, 20(5), 571-586. 
[30] Smith JL, Paul EA (1988). Use of an in situ labeling technique for the determination of seasonal 14C distribution in Ponderosa pine. Plant and Soil, 106, 221-229. CrossRef
[31] Wang J (王建), Qian JL (钱君龙), Liang Z (梁中), Zhao XY (赵兴云), Shang ZY (商志远), Chen X (陈霞), Lu XM (陆小明) (2008). Sampling strategy for carbon isotope analysis of tree rings, a case study of Cryptomeria fortunei from Mt. Tianmu, China. Acta Ecologica Sinica (生态学报), 28(12), 6070-6078. CrossRef
[32] Wang XC (王晓春), Song LP (宋来萍), Zhang YD (张远东) (2011). Climate-tree growth relationships of Pinus sylvestris var. mongolica in the northern Daxing’an Mountains, China. Acta Phytoecologica Sinica (植物生态学报), 35(3), 294-302. CrossRef
[33] Weigl M, Grabner M, Helle G, Schleser GH, Wimmer R (2008). Characteristics of radial growth and stable isotopes in a single oak tree to be used in climate studies. Science of the Total Environment, 393, 154-161. CrossRef
[34] Wilson AT, Grinsted JM (1977). 12C/13C in cellulose and lignin as paleothermometers. Nature, 265, 133-135. CrossRef
[35] Zhao XL (赵兴梁), Li WY (李万英) (1963). Pinus sylvestris var. mongolica Litv. (樟子松). 1st edn. China Agricultural Publishing House, Beijing. 9-10. 
No related articles found!
Full text



[1] . [J]. Chin Bull Bot, 2002, 19(01): 121 -124 .
[2] ZHANG Shi-Gong;GAO Ji-Yin and SONG Jing-Zhi. Effects of Betaine on Activities of Membrane Protective Enzymes in Wheat (Triticum aestivum L.) Seedlings Under NaCl Stress[J]. Chin Bull Bot, 1999, 16(04): 429 -432 .
[3] HE Wei-Ming and ZHONG Zhang-Cheng. Effects of Soil Fertility on Gynostemma pentaphyllum Makino Population Behavior[J]. Chin Bull Bot, 1999, 16(04): 425 -428 .
[4] SHE Chao-WenSONG Yun-Chun LIU Li-Hua. Analysis on the G_banded Karyotypes and Its Fluctuation at Different Mitotic Phases and Stages in Triticum tauschii (Aegilops squarrosa)[J]. Chin Bull Bot, 2001, 18(06): 727 -734 .
[5] Guijun Yang, Wenjiang Huang, Jihua Wang, Zhurong Xing. Inversion of Forest Leaf Area Index Calculated from Multi-source and Multi-angle Remote Sensing Data[J]. Chin Bull Bot, 2010, 45(05): 566 -578 .
[6] Man Chen, YishengTu, Linan Ye, Biyun Yang. Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata[J]. Chin Bull Bot, 2017, 52(2): 218 -224 .
[7] Yefei Shang, Ming Li, Bo Ding, Hao Niu, Zhenning Yang, Xiaoqiang Chen, Gaoyi Cao, Xiaodong Xie. Advances in Auxin Regulation of Plant Stomatal Development[J]. Chin Bull Bot, 2017, 52(2): 235 -240 .
[8] CUI Xiao-Yong, Du Zhan-Chi, Wang Yan-Fen. Photosynthetic Characteristics of a Semi-arid Sandy Grassland Community in Inner Mongolia[J]. Chin J Plan Ecolo, 2000, 24(5): 541 -546 .
[9] LI Wei, ZHANG Ya-Li, HU Yuan-Yuan, YANG Mei-Sen, WU Jie, and ZHANG Wang-Feng. Research on the photoprotection and photosynthesis characteristics of young cotton leaves under field conditions[J]. Chin J Plan Ecolo, 2012, 36(7): 662 -670 .
[10] HU Bao-Zhong, LIU Di, HU Guo-Fu, ZHANG A-Ying, JIANG Shu-Jun. Random Amplified Polymorphic DNA Study of Local Breeds in Chinese lfalfa[J]. Chin J Plan Ecolo, 2000, 24(6): 697 -701 .