EFFECTS OF INOCULATION OF ECTOMYCORRHIZAL FUNGI ON THE SEEDLING GROWTH OF MONGOL SCOTCH PINE (PINUS SYLVESTRISVAR. MONGOLICA)

doi:10.3773/j.issn.1005-264x.2008.06.019

Abstract

Abstract:

Aims Ectomycorrhizal fungi promote growth of Mongol Scotch pine (Pinus sylvestrisvar. mongolica) seedlings. Our objective was to determine the effects of different inoculation methods, different combinations of fungal strains and different single strains on growth of Mongol Scotch pine seedlings.

Methods We screened combinations of different ectomycorrhizal fungus strains by dual culture method. Two-year-old seedlings were inoculated with different single strains of ectomycorrhizal fungi by soaking roots in the liquid culture of the ectomycorrhizal fungus. Three-year-old seedlings were inoculated with different single strains using lister method in the field. Seedlings inoculated by PD liquid culture medium served as a control. Three-year-old seedlings were inoculated by different combinations of ectomycorrhizal fungus strains using lister method in the field, and those inoculated by different single strains served as a control.

Important findings All single strain and their combinations promoted the growth of Mongol Scotch pine seedlings. Strains GT005 and 035 had the largest growth-promoting effects in all combinations and single strain. Compared with the control, average heights of 2-year-old seedlings inoculated by strains GT005 and 035 increased 54% and 42%, respectively, and average collar diameters increased 15% and 56%, respectively. Average heights of 3-year-old seedlings inoculated by strains GT005 and 035 increased 10% and 7%, respectively, and average collar diameters increased 10% and 9%, respectively. The growth-promoting effect of the inoculating method of root soaking is greater than that of the lister inoculating method. When mixed with another strain, the effect of the high-effect strain on growth of seedlings was reduced. The activity of hydrogen peroxidase and root activity are not correlated with seedling biomass.

Key words: seedlings of Pinus sylvestris var. mongolica, ectomycorrhizal fungi, inoculation method, single-inoculation, mixed-inoculation, growth-promoting effect

SONG Rui-Qing, LI Xi-Mei, QI Jin-Yu. EFFECTS OF INOCULATION OF ECTOMYCORRHIZAL FUNGI ON THE SEEDLING GROWTH OF MONGOL SCOTCH PINE (PINUS SYLVESTRISVAR. MONGOLICA)[J]. Chin J Plant Ecol, 2008, 32(6): 1378-1385.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2008.06.019

https://www.plant-ecology.com/EN/Y2008/V32/I6/1378

Figures/Tables 6

References 11

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菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase		根系活力 Root activity	叶绿素含量 Content of chlorophyll
菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase		根系活力 Root activity	a	b		a+b		a/b
010 LH004 GT005 035 025 044 004 GT001 009	25.42* 35.59* 54.24* 41.53* 23.73* 33.90* 14.07* 35.59* 36.44*	26.28* 39.93* 14.68* 56.31* 29.01* 17.41* 10.92* 21.50* 1.71	-14.88 33.02* 48.37* 46.98* 42.33* 44.19* 37.21* 30.23* 45.12*		-5.83 8.33 -2.5 10.83* 10.83* 5.83 39.17* 2.5 16.67*	6.03 1.89 22.20* --- --- --- --- --- ---	21.15* 11.11* 24.20* --- 16.34* --- 7.18 6.83 ---		9.57* 4.02 22.67* --- --- --- 0.44 --- ---		2.868 3.005 3.224 2.961 2.418 2.919 2.850 3.020 2.896

菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase		根系活力 Root activity	叶绿素含量 Content of chlorophyll
菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase		根系活力 Root activity	a	b		a+b		a/b
010 LH004 GT005 035 025 044 004 GT001 009	25.42* 35.59* 54.24* 41.53* 23.73* 33.90* 14.07* 35.59* 36.44*	26.28* 39.93* 14.68* 56.31* 29.01* 17.41* 10.92* 21.50* 1.71	-14.88 33.02* 48.37* 46.98* 42.33* 44.19* 37.21* 30.23* 45.12*		-5.83 8.33 -2.5 10.83* 10.83* 5.83 39.17* 2.5 16.67*	6.03 1.89 22.20* --- --- --- --- --- ---	21.15* 11.11* 24.20* --- 16.34* --- 7.18 6.83 ---		9.57* 4.02 22.67* --- --- --- 0.44 --- ---		2.868 3.005 3.224 2.961 2.418 2.919 2.850 3.020 2.896

菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	根系活力 Root activity	叶绿素含量 Content of chlorophyll
菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	根系活力 Root activity	a	b	a+b	a/b
010 LH004 GT005 035 025 044 004 GT001 009	4.58 -7.83 9.96* 6.80 0.68 -0.47 3.50 1.64 1.71	10.64 -2.97 15.18* 8.92 2.66 9.86 3.91 0.47 3.13	10.48 12.90 90.32* 5.64 -16.13 72.58* 45.97* 167.74* 30.64*	-29.00 -36.30 -34.14 -46.49 20.21* -4.86 -17.96 30.87* -23.01	8.91* 0.14 30.95* 11.48* 10.31* 12.12* 0.56 -6.84 7.41*	35.42* -0.79 56.48* 44.93* 30.53* 44.23* 28.09* 5.50 29.50*	15.46* -0.08 37.25* 19.75* 15.31* 20.06* 7.37* -3.78 12.89*	-21.86 0.00 -16.85 -23.43 -16.00 -22.51 -22.71 -11.98 -41.82

菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	根系活力 Root activity	叶绿素含量 Content of chlorophyll
菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	根系活力 Root activity	a	b	a+b	a/b
010 LH004 GT005 035 025 044 004 GT001 009	4.58 -7.83 9.96* 6.80 0.68 -0.47 3.50 1.64 1.71	10.64 -2.97 15.18* 8.92 2.66 9.86 3.91 0.47 3.13	10.48 12.90 90.32* 5.64 -16.13 72.58* 45.97* 167.74* 30.64*	-29.00 -36.30 -34.14 -46.49 20.21* -4.86 -17.96 30.87* -23.01	8.91* 0.14 30.95* 11.48* 10.31* 12.12* 0.56 -6.84 7.41*	35.42* -0.79 56.48* 44.93* 30.53* 44.23* 28.09* 5.50 29.50*	15.46* -0.08 37.25* 19.75* 15.31* 20.06* 7.37* -3.78 12.89*	-21.86 0.00 -16.85 -23.43 -16.00 -22.51 -22.71 -11.98 -41.82

菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	Root activity	叶绿素含量 Content of chlorophyll
菌株 Strains	苗高 Seedlings Height	地径 Seedlings collar diameter	过氧化氢酶活性 Activity of hydrogen peroxidase	Root activity	a	b	a+b	a/b
044/025 044/009 044/GT001 GT001/GT005	-1.02 -4.47 3.47 2.07	7.98 0.16 4.69 4.38	64.51* 15.32 69.35* 27.42*	-15.62 1.87 -20.30 24.42*	14.93* -14.84 43.36* 15.24*	32.31* -0.66 94.00* 27.14*	19.23* -3.78 -11.33 18.18*	-13.29 -11.98 -14.33 -9.65