Chin J Plant Ecol ›› 2023, Vol. 47 ›› Issue (9): 1298-1309.DOI: 10.17521/cjpe.2022.0480

• Research Articles • Previous Articles     Next Articles

Species composition and driving factors of the ectomycorrhizal fungal community associated with Pinus sylvestris var. mongolica at different growth periods

REN Yue1, GAO Guang-Lei1,2,3,*(), DING Guo-Dong1,2,3, ZHANG Ying1,2, ZHAO Pei-Shan1, LIU Ye1   

  1. 1School of Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China
    2Engineering Research Center of Forestry Ecological Engineering, Ministry of Education, Key Laboratory of State Forestry and Grassland Administration on Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China
    3Yanchi Ecology Research Station of the Mu Us Sandy Land, Yanchi, Ningxia 751500, China
  • Received:2022-11-28 Accepted:2023-04-26 Online:2023-09-20 Published:2023-09-28
  • Contact: * GAO Guang-Lei(
  • Supported by:
    Science and Technology Project of Nei Mongol Autonomous Region(2022YFHH0131);Fundamental Research Funds for the Central Universities(2021ZY47)


Aims This study aimed to illustrate community structure, molecular ecological network and driving factors of ectomycorrhizal fungi associated with Pinus sylvestris var. mongolica at different growth periods, and provide a theoretical basis for sustainable management of P. sylvestris var. mongolica plantation.
Methods Pinus sylvestris var. mongolica with different tree ages (23, 33 and 44 a) in Mau Us Sandy Land were targeted. The species compositions, interaction and main drivers of ectomycorrhizal fungi at the early growth period (Apr.), vigorous growth period (July) and the late growth period (Sept.) were identified by field investigation and sampling, illumina high-throughput sequencing and molecular ecological network analysis, respectively.
Important findings 1) The growth period had significant effect on the richness and Chao1 index of ectomycorrhizal fungi, with significantly higher Chao1 and Simpson diversity index in vigorous growth period than in early and late growth periods. The tree age had no significant effect on the ectomycorrhizal fungal diversity index. 2) In Mau Us Sandy Land, the ectomycorrhizal fungi associated with P. sylvestris var. mongolica were identified into 2 phyla, 4 classes, 7 orders, 18 families, and 28 genera. The dominant genera were Tomentella, Inocybe and Geopora at the early, vigorous and late growth periods, respectively. The Tomentella and Inocybe were the common indicator genera in both vigorous and late growth periods. 3) At the end of the growing season, the nodes and edges of the ectomycorrhizal fungal networks were the largest, indicating that the ectomycorrhizal fungal community was more complex and there was strong interaction between fungal species. 4) Soil pH and annual relative humidity were the key environmental factors that significantly affected the community composition of ectomycorrhizal fungi. Our results demonstrated that the diversity and community composition of ectomycorrhizal fungi were affected by the growth stage and tree age, with a stronger effect in growth stage than in tree age. The seasonal dynamic distribution of ectomycorrhizal fungi mainly depended on soil properties and climate conditions.

Key words: Pinus sylvestris var. mongolica, ectomycorrhizal fungi, species composition, molecular ecological network