Species composition and driving factors of the ectomycorrhizal fungal community associated with Pinus sylvestris var. mongolica at different growth periods

doi:10.17521/cjpe.2022.0480

Abstract

Abstract:

Aims This study aimed to illustrate community structure, molecular ecological network and driving factors of ectomycorrhizal fungi associated with Pinus sylvestris var. mongolica at different growth periods, and provide a theoretical basis for sustainable management of P. sylvestris var. mongolica plantation.
Methods Pinus sylvestris var. mongolica with different tree ages (23, 33 and 44 a) in Mau Us Sandy Land were targeted. The species compositions, interaction and main drivers of ectomycorrhizal fungi at the early growth period (Apr.), vigorous growth period (July) and the late growth period (Sept.) were identified by field investigation and sampling, illumina high-throughput sequencing and molecular ecological network analysis, respectively.
Important findings 1) The growth period had significant effect on the richness and Chao1 index of ectomycorrhizal fungi, with significantly higher Chao1 and Simpson diversity index in vigorous growth period than in early and late growth periods. The tree age had no significant effect on the ectomycorrhizal fungal diversity index. 2) In Mau Us Sandy Land, the ectomycorrhizal fungi associated with P. sylvestris var. mongolica were identified into 2 phyla, 4 classes, 7 orders, 18 families, and 28 genera. The dominant genera were Tomentella, Inocybe and Geopora at the early, vigorous and late growth periods, respectively. The Tomentella and Inocybe were the common indicator genera in both vigorous and late growth periods. 3) At the end of the growing season, the nodes and edges of the ectomycorrhizal fungal networks were the largest, indicating that the ectomycorrhizal fungal community was more complex and there was strong interaction between fungal species. 4) Soil pH and annual relative humidity were the key environmental factors that significantly affected the community composition of ectomycorrhizal fungi. Our results demonstrated that the diversity and community composition of ectomycorrhizal fungi were affected by the growth stage and tree age, with a stronger effect in growth stage than in tree age. The seasonal dynamic distribution of ectomycorrhizal fungi mainly depended on soil properties and climate conditions.

Key words: Pinus sylvestris var. mongolica, ectomycorrhizal fungi, species composition, molecular ecological network

REN Yue, GAO Guang-Lei, DING Guo-Dong, ZHANG Ying, ZHAO Pei-Shan, LIU Ye. Species composition and driving factors of the ectomycorrhizal fungal community associated with Pinus sylvestris var. mongolica at different growth periods[J]. Chin J Plant Ecol, 2023, 47(9): 1298-1309.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2022.0480

https://www.plant-ecology.com/EN/Y2023/V47/I9/1298

Figures/Tables 10

References 48

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龄组 Age group	林龄 Stand age (a)	纬度 Longitude (° N)	经度 Longitude (° E)	平均树高 Average tree height (m)	平均胸径 Average tree DBH (cm)	郁闭度 Canopy density
中龄林 MUh	23	38.33	109.72	9.91 ± 1.02	14.15 ± 3.05	0.72
近熟林 MUn	33	38.34	109.73	13.05 ± 0.94	18.63 ± 2.46	0.80
成熟林 MUm	44	38.34	109.73	14.65 ± 0.81	21.98 ± 3.04	0.76

龄组 Age group	林龄 Stand age (a)	纬度 Longitude (° N)	经度 Longitude (° E)	平均树高 Average tree height (m)	平均胸径 Average tree DBH (cm)	郁闭度 Canopy density
中龄林 MUh	23	38.33	109.72	9.91 ± 1.02	14.15 ± 3.05	0.72
近熟林 MUn	33	38.34	109.73	13.05 ± 0.94	18.63 ± 2.46	0.80
成熟林 MUm	44	38.34	109.73	14.65 ± 0.81	21.98 ± 3.04	0.76

生长期 Growth period	龄组 Age group	丰富度 Richness	Chao1指数 Chao1 index	ACE指数 ACE index	Shannon-Wiener指数 Shannon-Wiener index	Simpson指数 Simpson index	Pielou指数 Pielou index
Apr.	MUh	13 ± 1^Ba	15.78 ± 2.91^Ba	16.44 ± 0.81^Aa	1.64 ± 0.25^Aa	0.75 ± 0.05^Aa	2.64 ± 0.93^Aa
	MUn	13 ± 1^Ba	14.61 ± 2.96^Ba	20.22 ± 2.17^Aa	1.15 ± 0.45^Aa	0.56 ± 0.17^Aa	1.93 ± 0.49^Ba
	MUm	12 ± 1^Ba	17.00 ± 7.86^Ba	24.65 ± 1.43^Aa	1.02 ± 0.32^Aa	0.46 ± 0.19^Aa	2.43 ± 0.48^Aa
July	MUh	24 ± 5^Aa	28.75 ± 8.74^Aa	35.83 ± 2.72^Aa	1.77 ± 0.19^Aa	0.78 ± 0.03^Aa	3.00 ± 0.83^Aa
	MUn	22 ± 2^Aa	26.67 ± 3.06^Aa	26.62 ± 3.04^Aa	1.38 ± 0.28^Aa	0.64 ± 0.09^Aa	2.60 ± 0.27^ABa
	MUm	23 ± 4^Aa	27.17 ± 6.95^Aa	30.44 ± 3.65^Aa	1.56 ± 0.30^Aa	0.70 ± 0.11^Aa	2.83 ± 0.53^Aa
Sept.	MUh	19 ± 3^Aa	19.92 ± 3.02^Aa	20.94 ± 3.42^Aa	1.16 ± 0.46^Aa	0.54 ± 0.19^Ba	2.63 ± 0.50^Aa
	MUn	23 ± 1^Aa	24.92 ± 1.13^Aa	29.09 ± 3.09^Aa	1.53 ± 0.53^Aa	0.66 ± 0.22^Aa	2.95 ± 0.13^Aa
	MUm	19 ± 5^Aa	26.83 ± 11.73^Aa	26.45 ± 2.41^Aa	1.18 ± 0.54^Aa	0.54 ± 0.20^Aa	2.48 ± 0.77^Aa
F_{growth period}		31.705^**	6.606^**	1.804	1.676	1.955	1.424
F_{age group}		0.462	0.235	0.130	1.075	1.485	0.204
F_{growth period × age group}		1.051	0.352	0.538	1.296	1.249	1.003

生长期 Growth period	龄组 Age group	丰富度 Richness	Chao1指数 Chao1 index	ACE指数 ACE index	Shannon-Wiener指数 Shannon-Wiener index	Simpson指数 Simpson index	Pielou指数 Pielou index
Apr.	MUh	13 ± 1^Ba	15.78 ± 2.91^Ba	16.44 ± 0.81^Aa	1.64 ± 0.25^Aa	0.75 ± 0.05^Aa	2.64 ± 0.93^Aa
	MUn	13 ± 1^Ba	14.61 ± 2.96^Ba	20.22 ± 2.17^Aa	1.15 ± 0.45^Aa	0.56 ± 0.17^Aa	1.93 ± 0.49^Ba
	MUm	12 ± 1^Ba	17.00 ± 7.86^Ba	24.65 ± 1.43^Aa	1.02 ± 0.32^Aa	0.46 ± 0.19^Aa	2.43 ± 0.48^Aa
July	MUh	24 ± 5^Aa	28.75 ± 8.74^Aa	35.83 ± 2.72^Aa	1.77 ± 0.19^Aa	0.78 ± 0.03^Aa	3.00 ± 0.83^Aa
	MUn	22 ± 2^Aa	26.67 ± 3.06^Aa	26.62 ± 3.04^Aa	1.38 ± 0.28^Aa	0.64 ± 0.09^Aa	2.60 ± 0.27^ABa
	MUm	23 ± 4^Aa	27.17 ± 6.95^Aa	30.44 ± 3.65^Aa	1.56 ± 0.30^Aa	0.70 ± 0.11^Aa	2.83 ± 0.53^Aa
Sept.	MUh	19 ± 3^Aa	19.92 ± 3.02^Aa	20.94 ± 3.42^Aa	1.16 ± 0.46^Aa	0.54 ± 0.19^Ba	2.63 ± 0.50^Aa
	MUn	23 ± 1^Aa	24.92 ± 1.13^Aa	29.09 ± 3.09^Aa	1.53 ± 0.53^Aa	0.66 ± 0.22^Aa	2.95 ± 0.13^Aa
	MUm	19 ± 5^Aa	26.83 ± 11.73^Aa	26.45 ± 2.41^Aa	1.18 ± 0.54^Aa	0.54 ± 0.20^Aa	2.48 ± 0.77^Aa
F_{growth period}		31.705^**	6.606^**	1.804	1.676	1.955	1.424
F_{age group}		0.462	0.235	0.130	1.075	1.485	0.204
F_{growth period × age group}		1.051	0.352	0.538	1.296	1.249	1.003

组别 Group	指示种 Indicator species	属 Genus	指示值 Indicator value	p
Apr.	OTU216	盾盘菌属 Scutellinia	0.970	0.001
July	OTU27	茸盖亚属 Mallocybe	0.976	0.001
	OTU104	茸盖亚属 Mallocybe	0.960	0.003
	OTU20	丝盖伞属 Inocybe	0.975	0.001
	OTU88	丝盖伞属 Inocybe	0.957	0.003
	OTU142	丝盖伞属 Inocybe	0.930	0.001
	OTU892	阿太菌属 Amphinema	0.879	0.001
	OTU368	棉革菌属 Tomentella	0.846	0.028
	OTU441	棉革菌属 Tomentella	0.789	0.047
Sept.	OTU207	丝盖伞属 Inocybe	0.922	0.006
	OTU420	棉革菌属 Tomentella	0.839	0.024
	OTU264	地孔菌属 Geopora	0.815	0.001