Soil fungal community characteristics at the upper and lower altitudinal range limits of Cephalostachyum pingbianense

doi:10.17521/cjpe.2021.0200

Abstract

Abstract:

Aims The formation of the geographical range boundary of species has always been an important topic in evolutionary biology. Although plant-microbe interactions have been extensively studied, we have a poor understanding of how plant's geographic range limits affect soil microorganisms. Cephalostachyum pingbianense is a rare bamboo species documented that produces bamboo shoots all year round in the wild, and is endemic to southeast Yunnan Province, China. The species is of great significance to study narrow endemic species in Bambusoideae. Here, we aim to reveal the relationship between the range limits of C. pingbianenseand soil fungal community.

Methods We assayed soil physical and chemical properties at the center, edge and beyond the range of C. pingbianense, and changes of fungal community were analyzed by means of Internal Transcribed Spacer (ITS) sequence based Illumina MiSeq high-throughput sequencing techniques.

Important findings (1) Soil pH and available phosphorus content at the range edges was significantly lower than other sites. (2) At the range center, species diversity of soil fungi was the highest, and relative abundance of Mortierella was significantly higher than other sites. At the range edges, species diversity of soil fungi was the lowest, and relative abundance of Basidiomycota was greater than 65.0%. (3) Soil pH played a crucial role in driving the variation of fungal community, which was negatively correlated with the relative abundance of ectomycorrhizal fungi, and positively correlated with the relative abundance of saprophytic fungi. Soil acidification and phosphorus deficiency may be important soil properties controlling the distribution range of C. pingbianense. Mortierella may be important mutualists of C. pingbianense, which can desorb phosphorus from soil minerals and reduce acidification of soil.

Key words: Cephalostachyum pingbianense, range limit, soil physicochemical properties, fungi, Mortierella

XIA Ti-Ze, LI Lu-Shuang, YANG Han-Qi. Soil fungal community characteristics at the upper and lower altitudinal range limits of Cephalostachyum pingbianense[J]. Chin J Plant Ecol, 2022, 46(7): 823-833.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2021.0200

https://www.plant-ecology.com/EN/Y2022/V46/I7/823

Figures/Tables 9

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指标 Index	A	B	C	D	E
pH	5.06 ± 0.17^a	3.65 ± 0.32^c	4.49 ± 0.18^b	4.04 ± 0.12^c	4.67 ± 0.12^b
有机质含量 SOM content (g·kg^-1)	79.20 ± 6.67^b	120.90 ± 7.24^a	121.84 ± 6.49^a	128.75 ± 12.61^a	128.05 ± 9.39^a
全氮含量 TN content (g·kg^-1)	3.89 ± 0.53^d	6.36 ± 1.84^b	4.48 ± 0.36^c	7.10 ± 0.11^a	6.55 ± 0.74^b
速效氮含量 AN content (g·kg^-1)	0.56 ± 0.07^c	1.08 ± 0.25^a	0.61 ± 0.04^bc	0.76 ± 0.19^b	0.74 ± 0.06^b
全磷含量 TP content (g·kg^-1)	0.52 ± 0.02^b	0.59 ± 0.07^b	0.68 ± 0.05^b	0.53 ± 0.04^b	0.94 ± 0.10^a
速效磷含量 AP content (mg·kg^-1)	12.58 ± 1.02^b	9.96 ± 0.82^c	13.03 ± 0.70^b	8.43 ± 3.41^c	18.04 ± 6.67^a
全钾含量 TK content (g·kg^-1)	16.59 ± 1.37^a	12.49 ± 2.40^b	16.02 ± 0.53^ab	12.46 ± 2.58^b	14.77 ± 1.33^b
速效钾含量 AK content (g·kg^-1)	0.16 ± 0.01^b	0.19 ± 0.01^a	0.15 ± 0.02^b	0.17 ± 0.05^ab	0.19 ± 0.02^a

指标 Index	A	B	C	D	E
pH	5.06 ± 0.17^a	3.65 ± 0.32^c	4.49 ± 0.18^b	4.04 ± 0.12^c	4.67 ± 0.12^b
有机质含量 SOM content (g·kg^-1)	79.20 ± 6.67^b	120.90 ± 7.24^a	121.84 ± 6.49^a	128.75 ± 12.61^a	128.05 ± 9.39^a
全氮含量 TN content (g·kg^-1)	3.89 ± 0.53^d	6.36 ± 1.84^b	4.48 ± 0.36^c	7.10 ± 0.11^a	6.55 ± 0.74^b
速效氮含量 AN content (g·kg^-1)	0.56 ± 0.07^c	1.08 ± 0.25^a	0.61 ± 0.04^bc	0.76 ± 0.19^b	0.74 ± 0.06^b
全磷含量 TP content (g·kg^-1)	0.52 ± 0.02^b	0.59 ± 0.07^b	0.68 ± 0.05^b	0.53 ± 0.04^b	0.94 ± 0.10^a
速效磷含量 AP content (mg·kg^-1)	12.58 ± 1.02^b	9.96 ± 0.82^c	13.03 ± 0.70^b	8.43 ± 3.41^c	18.04 ± 6.67^a
全钾含量 TK content (g·kg^-1)	16.59 ± 1.37^a	12.49 ± 2.40^b	16.02 ± 0.53^ab	12.46 ± 2.58^b	14.77 ± 1.33^b
速效钾含量 AK content (g·kg^-1)	0.16 ± 0.01^b	0.19 ± 0.01^a	0.15 ± 0.02^b	0.17 ± 0.05^ab	0.19 ± 0.02^a

指标 Index	A	B	C	D	E
Shannon指数 Shannon index	4.37 ± 0.13^b	3.02 ± 0.48^c	4.84 ± 0.08^a	2.97 ± 0.02^c	4.31 ± 0.21^b
Chao1指数 Chao1 index	670 ± 160^c	1 163 ± 152^b	1 655 ± 147^a	1 199 ± 42^b	1 321 ± 190^ab
可操作分类单元 OTU	641 ± 130^c	935 ± 140^b	1 442 ± 95^a	917 ± 28^b	1 107 ± 117^b

指标 Index	A	B	C	D	E
Shannon指数 Shannon index	4.37 ± 0.13^b	3.02 ± 0.48^c	4.84 ± 0.08^a	2.97 ± 0.02^c	4.31 ± 0.21^b
Chao1指数 Chao1 index	670 ± 160^c	1 163 ± 152^b	1 655 ± 147^a	1 199 ± 42^b	1 321 ± 190^ab
可操作分类单元 OTU	641 ± 130^c	935 ± 140^b	1 442 ± 95^a	917 ± 28^b	1 107 ± 117^b

显著差异的真菌 Fungi with significant differences	A	B	C	D	E
担子菌门 Basidiomycota	30.04 ± 4.25^c	70.11 ± 3.32^a	30.13 ± 1.47^c	66.35 ± 3.80^a	50.84 ± 2.71^b
子囊菌门 Ascomycota	48.87 ± 5.07^a	20.63 ± 1.67^c	26.61 ± 1.93^b	25.42 ± 5.17^bc	27.94 ± 2.51^b
被孢霉门 Mortierellomycota	8.59 ± 4.27^bc	1.52 ± 0.13^c	23.78 ± 0.96^a	2.93 ± 2.23^c	14.49 ± 0.27^b
罗兹菌门 Rozellomycota	0.88 ± 0.32^c	1.08 ± 0.25^a	7.86 ± 0.89^a	2.88 ± 2.22^b	1.58 ± 0.08^b
被孢霉属 Mortierella	8.38 ± 4.11^bc	1.46 ± 0.13^c	23.29 ± 0.85^a	0.53 ± 0.04^c	11.46 ± 0.21^b
Saitozyma	15.40 ± 7.77^a	2.52 ± 1.52^b	15.44 ± 1.48^a	4.05 ± 3.10^b	11.67 ± 3.34^a
红菇属 Russula	0.51 ± 0.22^c	27.61 ± 22.57^a	0.50 ± 0.15^c	57.63 ± 3.03^a	10.02 ± 6.76^ab
产油菌属 Solicoccozyma	0.16 ± 0.15^b	0.45 ± 0.41^b	5.56 ± 1.52^a	0.27 ± 0.37^b	1.78 ± 0.82^b
珊瑚菌属 Clavaria	5.04 ± 3.59^a	0.03 ± 0.05^b	0.00 ± 0.00^b	0.02 ± 0.01^b	0.03 ± 0.01^b
绿僵菌属 Metarhizium	2.41 ± 0.53^b	0.75 ± 0.55^c	4.60 ± 0.79^a	0.27 ± 0.19^c	1.60 ± 0.51^bc
刺球菌属 Chaetosphaeria	0.48 ± 0.33^ab	0.50 ± 0.23^ab	1.16 ± 0.43^a	0.32 ± 0.06^b	1.24 ± 0.23^a
腾梗孢霉属 Gonytrichum	0.94 ± 0.84^b	0.02 ± 0.01^b	2.20 ± 0.33^a	0.09 ± 0.08^b	0.26 ± 0.13^b
织球壳属 Plectosphaerella	2.06 ± 0.40^a	0.03 ± 0.02^b	0.03 ± 0.09^b	0.02 ± 0.01^b	0.09 ± 0.02^b