Carbon transport and phosphorus uptake in an intercropping system of Robinia pseudoacacia and Amorphophallus konjac mediated by arbuscular mycorrhizal hyphal networks

doi:10.17521/cjpe.2022.0185

Abstract

Abstract:

Aims The aim of this research was to clarify the effects of intercropping and inoculation with arbuscular mycorrhizal fungi (AMF) on carbon transport and phosphorus uptake in black locust (Robinia pseudoacacia) and konjac (Amorphophallus konjac). The results could provide empirical evidence to reveal the mechanisms of black locust intercropping for disease control and plant growth promotion of konjac, and popularize the green and high-yielding cultivation technique of konjac under black locust.

Methods The experiment was carried out in two-compartment rhizoboxes separated by a 25-μm nylon net, each of which comprised compartment A (non-inoculated or AMF-inoculated black locust) and compartment B (monocropped black locust or intercropped konjac). A ¹³C stable isotope labeling technique was used to label the leaves of black locust in compartment A with ¹³CO₂. Carbon transport from black locust to konjac and the effects of AMF colonization on agronomic traits, ¹³C abundance, and phosphorus content in both crops were investigated.

Important findings The result showed that: (1) After inoculation, the AMF infection rate of black locust and konjac plants by hyphal links in compartment B reached 47.1% and 60.4%, respectively. For black locust, this AMF infection rate was 14.1% lower than that of directly inoculated plants under monocropping. In the case of intercropping, the biomass (dry mass) of AMF-inoculated konjac plants was 9.7% (aboveground parts) and 36.2% (belowground roots) higher than that of non-inoculated plants. (2) Compared with the non-inoculated plants under monocropping, the carbon fixed by photosynthesis of black locust plants in other treatments (non-inoculated + intercropping, inoculated + monocropping, and inoculated + intercropping) was mainly allocated to the plant roots and rhizosphere soil in compartment A, and more carbon passed through the nylon net in the form of root exudates to reach the rhizosphere of neighboring crop plants. (3) Compared with the respective non-inoculated controls, AMF inoculation in the monocropping and intercropping systems prominently improved phosphorus contents in the leaves, stems/petioles, roots, and total plants of black locust and konjac in compartment B. The findings suggest that AMF colonization could facilitate carbon transport from black locust to the rhizosphere soil and plant tissues of konjac. Intercropping konjac with black locust is an effective practice to promote AMF colonization and phosphorus uptake by both host plants.

Key words: Robinia pseudoacacia, Amorphophallus konjac, arbuscular mycorrhizal fungi, ¹³C abundance, root exudate

HE Fei, LI Chuan, Faisal SHAH, LU Xie-Min, WANG Ying, WANG Meng, RUAN Jia, WEI Meng-Lin, MA Xing-Guang, WANG Zhuo, JIANG Hao. Carbon transport and phosphorus uptake in an intercropping system of Robinia pseudoacacia and Amorphophallus konjac mediated by arbuscular mycorrhizal hyphal networks[J]. Chin J Plant Ecol, 2023, 47(6): 782-791.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2022.0185

https://www.plant-ecology.com/EN/Y2023/V47/I6/782

Figures/Tables 7

References 44

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处理 Treatment	A室植物 Plant in compartment A	A室处理 Treatment in compartment A	B室植物 Plant in compartment B	种植方式 Cropping pattern
M-AMF	刺槐 Robinia pseudoacacia	未接种 Non-inoculated	刺槐 R. pseudoacacia	单作 Monocropping
I-AMF	刺槐 R. pseudoacacia	未接种 Non-inoculated	魔芋 Amorphophallus konjac	间作 Intercropping
M+AMF	刺槐 R. pseudoacacia	接种 Inoculated	刺槐 R. pseudoacacia	单作 Monocropping
I+AMF	刺槐 R. pseudoacacia	接种 Inoculated	魔芋 A. konjac	间作 Intercropping

处理 Treatment	A室植物 Plant in compartment A	A室处理 Treatment in compartment A	B室植物 Plant in compartment B	种植方式 Cropping pattern
M-AMF	刺槐 Robinia pseudoacacia	未接种 Non-inoculated	刺槐 R. pseudoacacia	单作 Monocropping
I-AMF	刺槐 R. pseudoacacia	未接种 Non-inoculated	魔芋 Amorphophallus konjac	间作 Intercropping
M+AMF	刺槐 R. pseudoacacia	接种 Inoculated	刺槐 R. pseudoacacia	单作 Monocropping
I+AMF	刺槐 R. pseudoacacia	接种 Inoculated	魔芋 A. konjac	间作 Intercropping

分室 Compartment	植物 Plant	处理 Treatment	AMF侵染率 AMF infection rate (%)	丛枝丰度 Arbuscular abundance (%)	侵染强度 Infection intensity (%)
A室 Compartment A	刺槐 R. pseudoacacia	M+AMF	54.8 ± 3.2^b	2.9 ± 0.2^b	6.8 ± 0.7^b
A室 Compartment A	刺槐 R. pseudoacacia	I+AMF	62.0 ± 0.3^a	4.8 ± 0.2^a	10.0 ± 0.2^a
B室 Compartment B	刺槐 R. pseudoacacia	M+AMF	47.1 ± 2.0^c	1.0 ± 0.1^c	2.2 ± 0.2^c
B室 Compartment B	魔芋 A. konjac	I+AMF	60.4 ± 6.6^a	4.6 ± 0.2^a	9.5 ± 0.6^a

分室 Compartment	植物 Plant	处理 Treatment	AMF侵染率 AMF infection rate (%)	丛枝丰度 Arbuscular abundance (%)	侵染强度 Infection intensity (%)
A室 Compartment A	刺槐 R. pseudoacacia	M+AMF	54.8 ± 3.2^b	2.9 ± 0.2^b	6.8 ± 0.7^b
A室 Compartment A	刺槐 R. pseudoacacia	I+AMF	62.0 ± 0.3^a	4.8 ± 0.2^a	10.0 ± 0.2^a
B室 Compartment B	刺槐 R. pseudoacacia	M+AMF	47.1 ± 2.0^c	1.0 ± 0.1^c	2.2 ± 0.2^c
B室 Compartment B	魔芋 A. konjac	I+AMF	60.4 ± 6.6^a	4.6 ± 0.2^a	9.5 ± 0.6^a

分室 Compartment	植物 Plant	处理 Treatment	株高 Plant height (cm)	地径 Ground diameter (mm)	地上干质量 Aboveground dry mass (g·plant^-1)	根系干质量 Root dry mass (g·plant^-1)
A室 Compartment A	刺槐 R. pseudoacacia	M-AMF	6.67 ± 0.03^e	1.71 ± 0.02^c	1.15 ± 0.06^f	0.10 ± 0.01^g
	刺槐 R. pseudoacacia	I-AMF	6.94 ± 0.14^e	1.72 ± 0.02^c	1.42 ± 0.08^e	0.21 ± 0.03^ef
	刺槐 R. pseudoacacia	M+AMF	10.83 ± 0.09^cd	1.81 ± 0.01^c	2.78 ± 0.14^c	0.32 ± 0.05^d
	刺槐 R. pseudoacacia	I+AMF	11.83 ± 0.09^c	1.81 ± 0.02^c	2.82 ± 0.18^c	1.04 ± 0.05^c
B室 Compartment B	刺槐 R. pseudoacacia	M-AMF	6.83 ± 0.11^e	1.71 ± 0.01^c	1.12 ± 0.12^f	0.15 ± 0.04^fg
	魔芋 A. konjac	I-AMF	23.80 ± 1.10^b	13.30 ± 0.40^b	13.54 ± 0.14^b	1.99 ± 0.02^b
	刺槐 R. pseudoacacia	M+AMF	10.61 ± 0.12^d	1.80 ± 0.00^c	2.20 ± 0.05^d	0.23 ± 0.01^e
	魔芋 A. konjac	I+AMF	26.72 ± 1.33^a	15.11 ± 0.62^a	14.85 ± 0.11^a	2.71 ± 0.08^a