Arbuscular mycorrhizal fungal community characteristics and driving factors in different grassland types

doi:10.17521/cjpe.2023.0244

Abstract

Abstract:

Aims Arbuscular mycorrhizal fungi (AMF) are widely distributed in grassland ecosystems. They make great contribution to the productivity and stability of grassland ecosystems. Our purpose was to explore the AMF community distribution characteristics and driving mechanism in grassland ecosystems.

Methods This study was conducted in 18 different grassland types. We demonstrate the AMF community characteristics and driving factors by investigating plant community characteristics, analyzing soil physicochemical properties and AMF communities.

Important findings In different grassland types, the dominant genera of AMF community were Claroideoglomus, Septoglomus, Diversispora, Rhizophagus, Acaulospora, Glomus and Ambispora. In addition, there exist significant differences in speicies composition and diversity of AMF communities, and the AMF diversity and rare operational taxonomic unites (OTUs) in Stipa purpurea grassland was relatively higher than those of other grassland types. Results from the structural equation model showed that AMF community diversity was significantly influenced by plant community diversity and soil pH, and AMF community composition was significantly influenced by temperature, soil moisture content and plant community composition. To sum up, abundant AMF resources were contained within Chinese natural grassland, and the AMF community characteristics in different grassland types are significantly different. Meanwhile, plant communities have remarkable driving effect on AMF communities in grassland ecosystems. So protecting grassland plant diversity is of great importance for maintaining soil microbial community stability.

Key words: arbuscular mycorrhizal fungi, grassland ecosystem, community composition, diversity, driving factor

CHEN Ke-Yu, XING Sen, TANG Yu, SUN Jia-Hui, REN Shi-Jie, ZHANG Jing, JI Bao-Ming. Arbuscular mycorrhizal fungal community characteristics and driving factors in different grassland types[J]. Chin J Plant Ecol, 2024, 48(5): 660-674.

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Figures/Tables 8

References 66

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草地类型 Grassland type	经度 Longitude (° E)	纬度 Latitude (° N)	海拔 Altitude (m)	1-8月平均气温 Average air temperature of Jan. to Aug. (℃)	1-8月降水量 Precipitation of Jan. to Aug. (mm)
高山嵩草-垂穗披碱草草地 Carex parvula - Elymus nutans grassland	91.90	29.72	4 056	-4.29	576.73
高山嵩草-圆穗蓼草地 C. parvula - Bistorta macrophylla grassland	93.25	30.69	4 473	-9.73	328.00
蕨麻草地 Argentina anserina grassland	91.15	31.12	4 488	-2.51	316.85
高山早熟禾草地 Poa alpina grassland	91.90	29.73	4 050	-4.29	576.73
西藏嵩草草地 C. tibetikobresia grassland	93.23	31.92	4 543	-9.99	478.53
垂穗披碱草草地 E. nutans grassland	98.27	30.87	3 640	-4.42	579.03
火绒草草地 Leontopodium leontopodioides grassland	91.51	31.87	4 502	-3.21	392.97
紫花针茅草地 Stipa purpurea grassland	90.32	31.39	4 641	-2.54	340.49
紫花针茅-青藏薹草草地 S. purpurea - C. moorcroftii grassland	84.83	32.00	4 578	-1.43	92.64
沙生薹草草地 C. praeclara grassland	84.03	29.78	4 816	-8.25	186.31
丝颖针茅草地 S. capillacea grassland	84.03	29.77	4 836	-8.22	186.31
大花嵩草草地 C. nudicarpa grassland	88.47	28.21	4 700	0.44	271.86
羊草-短花针茅草地 Leymus chinensis - S. breviflora grassland	111.22	41.25	1 701	4.40	96.12
短花针茅-冷蒿草地 S. breviflora - Artemisia frigida grassland	111.22	41.26	1 690	4.39	95.89
羊草-糙隐子草草地 L. chinensis - Cleistogenes squarrosa grassland	116.34	44.27	1 075	3.00	136.15
大针茅草地 S. grandis grassland	116.44	43.48	1 377	2.71	132.78
羊草-狼针草草地 L. chinensis - S. baicalensis grassland	120.00	49.34	613	-0.18	116.59
狼针草草地 S. baicalensis grassland	119.98	49.60	644	-0.31	119.26

草地类型 Grassland type	经度 Longitude (° E)	纬度 Latitude (° N)	海拔 Altitude (m)	1-8月平均气温 Average air temperature of Jan. to Aug. (℃)	1-8月降水量 Precipitation of Jan. to Aug. (mm)
高山嵩草-垂穗披碱草草地 Carex parvula - Elymus nutans grassland	91.90	29.72	4 056	-4.29	576.73
高山嵩草-圆穗蓼草地 C. parvula - Bistorta macrophylla grassland	93.25	30.69	4 473	-9.73	328.00
蕨麻草地 Argentina anserina grassland	91.15	31.12	4 488	-2.51	316.85
高山早熟禾草地 Poa alpina grassland	91.90	29.73	4 050	-4.29	576.73
西藏嵩草草地 C. tibetikobresia grassland	93.23	31.92	4 543	-9.99	478.53
垂穗披碱草草地 E. nutans grassland	98.27	30.87	3 640	-4.42	579.03
火绒草草地 Leontopodium leontopodioides grassland	91.51	31.87	4 502	-3.21	392.97
紫花针茅草地 Stipa purpurea grassland	90.32	31.39	4 641	-2.54	340.49
紫花针茅-青藏薹草草地 S. purpurea - C. moorcroftii grassland	84.83	32.00	4 578	-1.43	92.64
沙生薹草草地 C. praeclara grassland	84.03	29.78	4 816	-8.25	186.31
丝颖针茅草地 S. capillacea grassland	84.03	29.77	4 836	-8.22	186.31
大花嵩草草地 C. nudicarpa grassland	88.47	28.21	4 700	0.44	271.86
羊草-短花针茅草地 Leymus chinensis - S. breviflora grassland	111.22	41.25	1 701	4.40	96.12
短花针茅-冷蒿草地 S. breviflora - Artemisia frigida grassland	111.22	41.26	1 690	4.39	95.89
羊草-糙隐子草草地 L. chinensis - Cleistogenes squarrosa grassland	116.34	44.27	1 075	3.00	136.15
大针茅草地 S. grandis grassland	116.44	43.48	1 377	2.71	132.78
羊草-狼针草草地 L. chinensis - S. baicalensis grassland	120.00	49.34	613	-0.18	116.59
狼针草草地 S. baicalensis grassland	119.98	49.60	644	-0.31	119.26

草地类型 Grassland type	盖度 Coverage	高度 Height	丰富度 Richness	Simpson多样性指数 Simpson diversity index	Shannon-Wiener多样性指数 Shannon-Wiener diversity index	Pielou均匀度指数 Pielou evenness index
高山嵩草-垂穗披碱草草地 Carex parvula - Elymus nutans grassland	98.40 ± 0.40^a	26.51 ± 1.46^c	9.20 ± 0.73^c	0.86 ± 0.01^ab	2.07 ± 0.05^cd	0.94 ± 0.01^ab
高山嵩草-圆穗蓼草地 C. parvula - Bistorta macrophylla grassland	98.00 ± 0.45^a	7.53 ± 0.26^hij	13.40 ± 0.51^a	0.89 ± 0.01^a	2.42 ± 0.02^a	0.94 ± 0.01^ab
蕨麻草地 Argentina anserina grassland	48.00 ± 1.64^e	7.01 ± 0.71^ij	2.80 ± 0.37^f	0.51 ± 0.04^hi	0.82 ± 0.10^jk	0.89 ± 0.02^abcd
高山早熟禾草地 Poa alpina grassland	94.20 ± 1.66^ab	12.01 ± 0.78^efg	12.40 ± 0.40^ab	0.89 ± 0.01^a	2.33 ± 0.03^ab	0.94 ± 0.02^ab
西藏嵩草草地 C. tibetikobresia grassland	95.60 ± 1.50^ab	4.71 ± 0.23^j	11.60 ± 1.03^b	0.85 ± 0.02^ab	2.19 ± 0.08^bc	0.90 ± 0.01^abcd
垂穗披碱草草地 E. nutans grassland	96.60 ± 0.51^ab	23.57 ± 1.62^cd	8.60 ± 0.51^c	0.84 ± 0.02^abc	1.87 ± 0.05^def	0.87 ± 0.01^bcde
火绒草草地 Leontopodium leontopodioides grassland	64.20 ± 2.29^d	10.55 ± 0.57^fgh	3.60 ± 0.24^f	0.59 ± 0.03^fg	1.08 ± 0.06^hi	0.90 ± 0.03^abcd
紫花针茅草地 Stipa purpurea grassland	48.00 ± 0.95^e	12.63 ± 0.18^efg	7.00 ± 0.45^d	0.78 ± 0.01^bcd	1.75 ± 0.05^efg	0.90 ± 0.01^abcd
紫花针茅-青藏薹草草地 S. purpurea - C. moorcroftii grassland	43.00 ± 3.74^ef	14.64 ± 0.21^e	3.00 ± 0.32^f	0.58 ± 0.01^gh	0.98 ± 0.01^hij	0.89 ± 0.01^abcd
沙生薹草草地 C. praeclara grassland	40.40 ± 1.44^fg	9.84 ± 0.74^ghi	3.40 ± 0.24^f	0.73 ± 0.06^de	1.00 ± 0.02 ^hij	0.85 ± 0.06^de
丝颖针茅草地 S. capillacea grassland	36.00 ± 1.30^g	13.91 ± 0.54^ef	3.00 ± 0.45^f	0.67 ± 0.02^ef	0.88 ± 0.16^ijk	0.81 ± 0.04^e
大花嵩草草地 C. nudicarpa grassland	29.00 ± 1.38^h	9.51 ± 1.28^ghi	2.40 ± 0.24^f	0.49 ± 0.03ⁱ	0.75 ± 0.07^k	0.91 ± 0.02^abcd
羊草-短花针茅草地 Leymus chinensis - S. breviflora grassland	43.40 ± 3.33^ef	20.44 ± 0.52^d	3.40 ± 0.24^f	0.67 ± 0.01^ef	1.15 ± 0.05^h	0.95 ± 0.01^a
短花针茅-冷蒿草地 S. breviflora - Artemisia frigida grassland	45.00 ± 1.70^ef	25.54 ± 1.35^c	3.00 ± 0.45^f	0.52 ± 0.05^ghi	0.90 ± 0.13^ijk	0.85 ± 0.01^cde
羊草-糙隐子草草地 L. chinensis - Cleistogenes squarrosa grassland	79.00 ± 2.63^c	31.59 ± 0.68^b	6.40 ± 0.24^de	0.77 ± 0.01^cd	1.65 ± 0.04^g	0.88 ± 0.01^abcde
大针茅草地 S. grandis grassland	65.60 ± 0.87^d	21.84 ± 1.40^d	5.40 ± 0.24^e	0.76 ± 0.01^cd	1.55 ± 0.03^g	0.92 ± 0.01^abcd
羊草-狼针草草地 L. chinensis - S. baicalensis grassland	95.20 ± 2.65^ab	36.32 ± 2.86^a	8.40 ± 0.24^c	0.83 ± 0.01^abc	1.93 ± 0.03^de	0.91 ± 0.01^abcd
狼针草草地 S. baicalensis grassland	91.20 ± 1.20^b	34.12 ± 0.46^ab	6.40 ± 0.68^de	0.79 ± 0.01^bcd	1.69 ± 0.09^fg	0.92 ± 0.01^abc

草地类型 Grassland type	盖度 Coverage	高度 Height	丰富度 Richness	Simpson多样性指数 Simpson diversity index	Shannon-Wiener多样性指数 Shannon-Wiener diversity index	Pielou均匀度指数 Pielou evenness index
高山嵩草-垂穗披碱草草地 Carex parvula - Elymus nutans grassland	98.40 ± 0.40^a	26.51 ± 1.46^c	9.20 ± 0.73^c	0.86 ± 0.01^ab	2.07 ± 0.05^cd	0.94 ± 0.01^ab
高山嵩草-圆穗蓼草地 C. parvula - Bistorta macrophylla grassland	98.00 ± 0.45^a	7.53 ± 0.26^hij	13.40 ± 0.51^a	0.89 ± 0.01^a	2.42 ± 0.02^a	0.94 ± 0.01^ab
蕨麻草地 Argentina anserina grassland	48.00 ± 1.64^e	7.01 ± 0.71^ij	2.80 ± 0.37^f	0.51 ± 0.04^hi	0.82 ± 0.10^jk	0.89 ± 0.02^abcd
高山早熟禾草地 Poa alpina grassland	94.20 ± 1.66^ab	12.01 ± 0.78^efg	12.40 ± 0.40^ab	0.89 ± 0.01^a	2.33 ± 0.03^ab	0.94 ± 0.02^ab
西藏嵩草草地 C. tibetikobresia grassland	95.60 ± 1.50^ab	4.71 ± 0.23^j	11.60 ± 1.03^b	0.85 ± 0.02^ab	2.19 ± 0.08^bc	0.90 ± 0.01^abcd
垂穗披碱草草地 E. nutans grassland	96.60 ± 0.51^ab	23.57 ± 1.62^cd	8.60 ± 0.51^c	0.84 ± 0.02^abc	1.87 ± 0.05^def	0.87 ± 0.01^bcde
火绒草草地 Leontopodium leontopodioides grassland	64.20 ± 2.29^d	10.55 ± 0.57^fgh	3.60 ± 0.24^f	0.59 ± 0.03^fg	1.08 ± 0.06^hi	0.90 ± 0.03^abcd
紫花针茅草地 Stipa purpurea grassland	48.00 ± 0.95^e	12.63 ± 0.18^efg	7.00 ± 0.45^d	0.78 ± 0.01^bcd	1.75 ± 0.05^efg	0.90 ± 0.01^abcd
紫花针茅-青藏薹草草地 S. purpurea - C. moorcroftii grassland	43.00 ± 3.74^ef	14.64 ± 0.21^e	3.00 ± 0.32^f	0.58 ± 0.01^gh	0.98 ± 0.01^hij	0.89 ± 0.01^abcd
沙生薹草草地 C. praeclara grassland	40.40 ± 1.44^fg	9.84 ± 0.74^ghi	3.40 ± 0.24^f	0.73 ± 0.06^de	1.00 ± 0.02 ^hij	0.85 ± 0.06^de
丝颖针茅草地 S. capillacea grassland	36.00 ± 1.30^g	13.91 ± 0.54^ef	3.00 ± 0.45^f	0.67 ± 0.02^ef	0.88 ± 0.16^ijk	0.81 ± 0.04^e
大花嵩草草地 C. nudicarpa grassland	29.00 ± 1.38^h	9.51 ± 1.28^ghi	2.40 ± 0.24^f	0.49 ± 0.03ⁱ	0.75 ± 0.07^k	0.91 ± 0.02^abcd
羊草-短花针茅草地 Leymus chinensis - S. breviflora grassland	43.40 ± 3.33^ef	20.44 ± 0.52^d	3.40 ± 0.24^f	0.67 ± 0.01^ef	1.15 ± 0.05^h	0.95 ± 0.01^a
短花针茅-冷蒿草地 S. breviflora - Artemisia frigida grassland	45.00 ± 1.70^ef	25.54 ± 1.35^c	3.00 ± 0.45^f	0.52 ± 0.05^ghi	0.90 ± 0.13^ijk	0.85 ± 0.01^cde
羊草-糙隐子草草地 L. chinensis - Cleistogenes squarrosa grassland	79.00 ± 2.63^c	31.59 ± 0.68^b	6.40 ± 0.24^de	0.77 ± 0.01^cd	1.65 ± 0.04^g	0.88 ± 0.01^abcde
大针茅草地 S. grandis grassland	65.60 ± 0.87^d	21.84 ± 1.40^d	5.40 ± 0.24^e	0.76 ± 0.01^cd	1.55 ± 0.03^g	0.92 ± 0.01^abcd
羊草-狼针草草地 L. chinensis - S. baicalensis grassland	95.20 ± 2.65^ab	36.32 ± 2.86^a	8.40 ± 0.24^c	0.83 ± 0.01^abc	1.93 ± 0.03^de	0.91 ± 0.01^abcd
狼针草草地 S. baicalensis grassland	91.20 ± 1.20^b	34.12 ± 0.46^ab	6.40 ± 0.68^de	0.79 ± 0.01^bcd	1.69 ± 0.09^fg	0.92 ± 0.01^abc

草地类型 Grassland type	TC (g·kg^-1)	TN (g·kg^-1)	TP (g·kg^-1)	NH₄⁺-N (mg·kg^-1)	NO₃^--N(mg·kg^-1)	AP (mg·kg^-1)	pH	土壤含水率 Soil water content (%)
高山嵩草-垂穗披碱草草地 Carex parvula - Elymus nutans grassland	13.70 ± 1.17^def	1.40 ± 0.10^fghi	0.54 ± 0.03^fg	1.80 ± 0.30^c	1.13 ± 0.36^e	0.95 ± 0.14^e	6.47 ± 0.08^e	13.44 ± 1.67^fgh
高山嵩草-圆穗蓼草地 C. parvula - Bistorta macrophylla grassland	52.92 ± 1.15^b	4.57 ± 0.08^c	0.77 ± 0.02^c	22.51 ± 4.72^b	2.53 ± 0.29^de	4.46 ± 0.07^cd	6.23 ± 0.04^ef	34.86 ± 1.22^ab
蕨麻草地 Argentina anserina grassland	59.90 ± 0.47^b	2.94 ± 0.04^de	0.67 ± 0.04^de	2.05 ± 0.29^c	42.20 ± 2.75^bc	4.73 ± 0.18^cd	7.93 ± 0.02^b	20.46 ± 0.47^def
高山早熟禾草地 Poa alpina grassland	59.88 ± 8.07^b	4.94 ± 0.57^bc	0.81 ± 0.04^c	11.15 ± 4.20^c	67.73 ± 36.92^b	8.71 ± 0.58^b	5.97 ± 0.36^fg	31.59 ± 6.07^abc
西藏嵩草草地 C. tibetikobresia grassland	61.66 ± 15.45^b	5.49 ± 1.40^abc	0.73 ± 0.08^cd	10.54 ± 2.40^c	23.58 ± 3.74^cde	8.49 ± 3.88^b	6.25 ± 0.11^ef	33.70 ± 7.89^abc
垂穗披碱草草地 E. nutans grassland	76.54 ± 3.34^a	6.56 ± 0.27^a	1.10 ± 0.01^a	9.34 ± 1.03^c	197.20 ± 5.05^a	9.79 ± 0.11^b	5.79 ± 0.02^g	38.18 ± 1.29^a
火绒草草地 Leontopodium leontopodioides grassland	22.88 ± 0.35^cde	2.04 ± 0.03^defgh	0.56 ± 0.02^f	3.25 ± 0.47^c	6.60 ± 0.76^de	1.98 ± 0.08^de	5.85 ± 0.08^g	16.65 ± 0.70^def
紫花针茅草地 Stipa purpurea grassland	13.98 ± 0.81^def	0.85 ± 0.06^hi	0.37 ± 0.01ⁱ	1.89 ± 0.29^c	7.71 ± 0.49^de	3.44 ± 0.16^de	7.86 ± 0.10^b	4.78 ± 0.91^h
紫花针茅-青藏薹草草地 S. purpurea - C. moorcroftii grassland	18.40 ± 0.41^de	1.89 ± 0.07^efgh	0.22 ± 0.01^j	1.69 ± 0.08^c	5.70 ± 0.30^de	1.74 ± 0.11^de	8.58 ± 0.02^a	4.91 ± 0.67^h
沙生薹草草地 C. praeclara grassland	4.16 ± 0.39^f	0.47 ± 0.02ⁱ	0.26 ± 0.02^j	1.33 ± 0.12^c	1.08 ± 0.17^e	2.83 ± 0.06^de	8.16 ± 0.06^b	11.24 ± 7.10^gh
丝颖针茅草地 S. capillacea grassland	14.70 ± 0.62^def	1.50 ± 0.05^fghi	0.52 ± 0.01^fg	4.05 ± 0.61^c	5.51 ± 0.45^de	4.07 ± 0.09^de	7.05 ± 0.03^cd	4.96 ± 0.84^h
大花嵩草草地 C. nudicarpa grassland	9.06 ± 0.28^ef	1.24 ± 0.03^ghi	0.61 ± 0.01^ef	2.13 ± 0.34^c	3.78 ± 0.42^de	7.22 ± 0.15^bc	8.16 ± 0.06^b	4.30 ± 0.28^h
羊草-短花针茅草地 Leymus chinensis - S. breviflora grassland	18.20 ± 0.30^de	2.17 ± 0.03^defg	0.42 ± 0.01^hi	1.46 ± 0.19^c	32.35 ± 0.99^cd	2.66 ± 0.10^de	7.18 ± 0.02^cd	10.32 ± 0.36^gh
短花针茅-冷蒿草地 S. breviflora - Artemisia frigida grassland	14.18 ± 0.33^def	1.68 ± 0.03^fgh	0.39 ± 0.01ⁱ	2.24 ± 0.44^c	11.51 ± 0.42^de	2.49 ± 0.12^de	6.80 ± 0.05^d	4.22 ± 0.11^h
羊草-糙隐子草草地 L. chinensis - Cleistogenes squarrosa grassland	23.44 ± 1.16^cd	2.54 ± 0.11^def	0.47 ± 0.01^gh	2.80 ± 0.36^c	3.19 ± 0.26^de	8.58 ± 0.45^b	7.27 ± 0.03^c	4.93 ± 0.18^h
大针茅草地 S. grandis grassland	18.32 ± 0.34^de	2.10 ± 0.04^defg	0.37 ± 0.02ⁱ	1.84 ± 0.15^c	4.56 ± 0.29^de	3.36 ± 0.22^de	6.88 ± 0.03^d	5.27 ± 0.18^h
羊草-狼针草草地 L. chinensis - S. baicalensis grassland	34.50 ± 1.40^c	3.14 ± 0.12^d	0.54 ± 0.01^fg	3.58 ± 0.23^c	42.29 ± 3.10^bc	3.24 ± 0.27^de	6.48 ± 0.07^e	26.76 ± 0.93^bcd
狼针草草地 S. baicalensis grassland	61.72 ± 1.81^b	5.77 ± 0.16^ab	0.89 ± 0.02^b	47.99 ± 11.17^a	40.09 ± 5.55^c	16.28 ± 1.32^a	6.54 ± 0.02^e	24.63 ± 0.81^cde