Variation in soil bacterial community across vegetation types in Dajinshan Island, Shanghai

doi:10.17521/cjpe.2023.0172

Abstract

Abstract:

Aims Revealing the response patterns of soil bacterial community to changes in vegetation type during secondary succession can improve our understanding of the mechanisms that structure the above- and below-ground interactions in ecosystems.

Methods To investigate how soil bacterial diversity, taxa network structure and biomarkers shift with vegetation succession, this study measured soil carbon, nitrogen and phosphorus contents, as well as soil bacterial community properties across shrubland, deciduous broadleaf forest and evergreen broadleaf forests, representing the early-, middle- and late-successional stages, respectively, on Dajinshan Island, Shanghai.

Important findings Soil nutrient contents were significantly higher in evergreen broadleaf forest than in deciduous broadleaf forest. However, soil bacterial diversity was significantly higher in deciduous broadleaf forest than in evergreen broadleaf forest, while soil nutrient content and bacterial diversity were medium in deciduous shrubland. The correlation network nodes, density and complexity of soil bacteria were highest in deciduous broadleaf forest, medium in deciduous shrubland, and lowest in evergreen broadleaf forest. The dominant soil bacteria in deciduous shrubland and broadleaf forest was Rhizobiales and Burkholderiales, respectively, which belong to functional group of nitrogen-fixing. The dominant soil bacterial in evergreen broadleaf forest were characterized by functional groups of pathogenicity and resistance such as Xanthomonadales and Thermogemmatisporales, and functional group associated with cellulose degradation such as Acidobacteriales. These results suggest that changes in plant species composition and soil nutrient availability during island vegetation succession can greatly reshape species diversity, community composition, interactive network structure and biomarkers of soil bacteria. In evergreen broadleaf forest, lowered soil bacterial diversity, simplified bacterial network structure, and emerged biomarkers of functional groups of pathogenicity and resistance suggest a response of belowground to the degraded trend of aboveground in the studied climax forest.

Key words: secondary succession, island, shrubland, forest, bacterial diversity, 16S rDNA

YANG An-Na, LI Zeng-Yan, MOU Ling, YANG Bai-Yu, SAI Bi-Le, ZHANG Li, ZHANG Zeng-Ke, WANG Wan-Sheng, DU Yun-Cai, YOU Wen-Hui, YAN En-Rong. Variation in soil bacterial community across vegetation types in Dajinshan Island, Shanghai[J]. Chin J Plant Ecol, 2024, 48(3): 377-389.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2023.0172

https://www.plant-ecology.com/EN/Y2024/V48/I3/377

Figures/Tables 10

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植被类型 Vegetation type	总氮含量 Total nitrogen content (g·kg^-1)	总磷含量 Total phosphorus content (g·kg^-1)	有机碳含量 Organic matter content (g·kg^-1)	速效氮含量 Available nitrogen content (mg·kg^-1)	速效磷含量 Available phosphorus content (mg·kg^-1)	速效钾含量 Available kalium content (mg·kg^-1)
落叶灌丛 Deciduous shrubland	0.61 ± 0.10^ab	0.11 ± 0.01^b	5.99 ± 1.37^ab	453.70 ± 154.30^ab	4.03 ± 1.00^ab	232.17 ± 29.60^a
落叶阔叶林 Deciduous broadleaf forest	0.49 ± 0.09^b	0.09 ± 0.01^b	4.94 ± 1.09^b	323.80 ± 69.20^b	4.12 ± 1.50^a	234.00 ± 75.30^a
常绿阔叶林 Evergreen broadleaf forest	0.66 ± 0.14^a	0.17 ± 0.04^a	7.86 ± 1.97^a	480.31 ± 132.08^a	14.10 ± 5.80^a	199.41 ± 45.20^a

植被类型 Vegetation type	总氮含量 Total nitrogen content (g·kg^-1)	总磷含量 Total phosphorus content (g·kg^-1)	有机碳含量 Organic matter content (g·kg^-1)	速效氮含量 Available nitrogen content (mg·kg^-1)	速效磷含量 Available phosphorus content (mg·kg^-1)	速效钾含量 Available kalium content (mg·kg^-1)
落叶灌丛 Deciduous shrubland	0.61 ± 0.10^ab	0.11 ± 0.01^b	5.99 ± 1.37^ab	453.70 ± 154.30^ab	4.03 ± 1.00^ab	232.17 ± 29.60^a
落叶阔叶林 Deciduous broadleaf forest	0.49 ± 0.09^b	0.09 ± 0.01^b	4.94 ± 1.09^b	323.80 ± 69.20^b	4.12 ± 1.50^a	234.00 ± 75.30^a
常绿阔叶林 Evergreen broadleaf forest	0.66 ± 0.14^a	0.17 ± 0.04^a	7.86 ± 1.97^a	480.31 ± 132.08^a	14.10 ± 5.80^a	199.41 ± 45.20^a

植被类型 Vegetation type	OTU编号 OTU code	门 Phylum	纲 Class	目 Order	节点度 Nodes degree
落叶灌丛 Deciduous shrubland	OTU137	拟杆菌门 Bacteroidetes	腐螺旋菌纲 Saprospirae	腐螺旋菌目 Saprospirales	19
	OTU34	酸杆菌门 Acidobacteria	DA052	Ellin6513	19
	OTU52	酸杆菌门 Acidobacteria	DA052	Ellin6513	19
落叶阔叶林 Deciduous broadleaf forest	OTU98	变形菌门 Proteobacteria	γ变形菌纲 Gamma proteobacteria	黄单胞菌 Xanthomonadales	54
常绿阔叶林 Evergreen broadleaf forest	OTU104	酸杆菌门 Acidobacteria	DA052	Ellin6513	6
常绿阔叶林 Evergreen broadleaf forest	OTU47	WPS-2	-	-	6

植被类型 Vegetation type	OTU编号 OTU code	门 Phylum	纲 Class	目 Order	节点度 Nodes degree
落叶灌丛 Deciduous shrubland	OTU137	拟杆菌门 Bacteroidetes	腐螺旋菌纲 Saprospirae	腐螺旋菌目 Saprospirales	19
	OTU34	酸杆菌门 Acidobacteria	DA052	Ellin6513	19
	OTU52	酸杆菌门 Acidobacteria	DA052	Ellin6513	19
落叶阔叶林 Deciduous broadleaf forest	OTU98	变形菌门 Proteobacteria	γ变形菌纲 Gamma proteobacteria	黄单胞菌 Xanthomonadales	54
常绿阔叶林 Evergreen broadleaf forest	OTU104	酸杆菌门 Acidobacteria	DA052	Ellin6513	6
常绿阔叶林 Evergreen broadleaf forest	OTU47	WPS-2	-	-	6

门 Phylum	落叶灌丛-落叶阔叶林 Deciduous shrubland - deciduous broadleaf forest		落叶阔叶林-常绿阔叶林 Deciduous broadleaf forest - evergreen broadleaf forest		落叶灌丛-常绿阔叶林 Deciduous shrubland - evergreen broadleaf forest
门 Phylum	t	p	t	p	t	p
变形菌门 Proteobacteria	-0.65	0.79	0.63	0.81	-0.09	0.99
酸杆菌门 Acidobacteria	-0.50	0.87	-0.81	0.70	-1.15	0.49
放线菌门 Actinobacteria	2.71	0.03	-1.01	0.58	1.73	0.21
WPS-2	-0.89	0.65	-5.04	0.00	-5.06	0.00
绿弯菌门 Chloroflexi	-0.76	0.73	-1.29	0.41	-1.80	0.19
浮霉菌门 Planctomycetes	2.31	0.07	-0.03	0.99	-2.17	0.10
厚壁菌门 Firmicutes	-1.84	0.18	1.03	0.56	-0.89	0.65
AD3	-1.35	0.38	-0.30	0.95	-1.53	0.29
疣微菌门 Verrucomicrobia	3.45	0.01	0.69	0.77	3.85	0.00