Chin J Plant Ecol

   

Effect of adding black carbon after fire on community structure of ectomycorrhizal fungi in Larix gmelinii forest

Tong-xin HU1,Bei Li2,Guang-Xin Li1,Yue-Xiao Ren1,Hai-Lei Ding1,Long SUN   

  • Received:2022-08-18 Revised:2022-11-08 Published:2022-11-11
  • Contact: Long SUN

Abstract: Aims Fire is an important disturbance factor in the northern forest ecosystem, and black carbon, as a product of fire, widely exists in most forest soils. Many studies have shown the positive impact of black carbon on plant yield, but little is known about the synergy between black carbon and microorganisms (such as mycorrhizal fungi) that promote plant growth. Ectomycorrhiza fungi (ECM) is a bridge between coniferous trees and soil in the northern forest ecosystem. Exploring the changes of ectomycorrhizal fungi community after fire can provide a theoretical basis for the carbon pool restoration and scientific management of the northern forest ecosystem after fire. Methods In this study, Larix gmelinii forest in the Da Hinggan Mountains was taken as the research object. Samples were taken at the beginning of the post fire growth season (June) and at the end of the growth season (September). ECM fungi were identified by high-throughput sequencing method to explore the changes and key influencing factors of ECM fungal community under the treatment of fire and black carbon addition during the growth season. Important findings The results showed that: (1) During the growing season, the ECM fungi treated by fire α-diversity decreased significantly by 31.52%, while the ECM fungi treated with black carbon addition α-diversity increased significantly by 49.02%. β-diversity analysis also showed that there were significant differences in the composition of ECM fungal community under fire and black carbon addition during the growing season. (2) During the growing season, the abundance of ECM fungi treated by fire decreased significantly by 46.35%, but the fire treatment had a significant impact on the increase of basidiomycetes species abundance, while black carbon addition treatment had an impact on the increase of ascomycetes and basidiomycetes species abundance. (3) During the growing season, the composition of ECM fungal community in Larix gmelini forest was significantly affected by soil pH value, water content and total nitrogen content in the fire and black carbon addition treatments, but it was positively correlated with soil pH value and total nitrogen and negatively correlated with soil water content in the black carbon addition treatment. The results of this study showed that fire reduced the diversity of ECM fungi in Larix gmelinii forest during the growing season, and significantly reduced the abundance of ECM fungi. However, the entry of black carbon after fire may change this negative effect after fire. By affecting the changes of soil pH and soil nitrogen content, it promotes the recovery of ECM fungi and increases its diversity. Therefore, the long-term impact of black carbon on ECM fungal community should be continuously monitored in the future, so as to improve the application of black carbon in forest management.

Key words: Fire, Larix gmelinii, Ectomycorrhizal fungi, Black carbon, Diversity