Chin J Plant Ecol ›› 2023, Vol. 47 ›› Issue (6): 792-803.DOI: 10.17521/cjpe.2022.0333

Special Issue: 菌根真菌

• Research Articles • Previous Articles     Next Articles

Effects of fire originated black carbon on species composition of ectomycorrhizal fungi in a Larix gmelinii forest in growing season

HU Tong-Xin, LI Bei, LI Guang-Xin, REN Yue-Xiao, DING Hai-Lei, SUN Long()   

  1. Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, School of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2022-08-18 Accepted:2022-11-11 Online:2023-06-20 Published:2022-11-11
  • Contact: * (
  • Supported by:
    National Natural Science Foundation of China(32001324);Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS20210370);Excellent Youth Joint Guidance Project of Heilongjiang Natural Science Foundation(LH2021C012)


Aims Fire is an important disturbance factor in the northern forest ecosystem, and black carbon, a product of fire, widely exists in most forest soils. Many studies have shown the positive impact of black carbon on plant yield, but little is known about the synergy between black carbon and microorganisms (such as mycorrhizal fungi) which can promote plant growth. Ectomycorrhiza (ECM) fungi is like a bridge between coniferous trees and soil in the northern forest ecosystem. Exploring the changes of ECM fungal community after fire can provide a theoretical basis for the carbon pool restoration and help with scientific management of the northern forest ecosystem after fire.

Methods In this study, Larix gmelinii forest in the Da Hinggan Mountains was taken as the research object. Samples were taken at the beginning of the growing season (June) and at the end of the growing season (September) after fire disturbance. ECM fungi were identified by high-throughput sequencing method to explore the changes and key influencing factors of ECM fungal community under the treatment of fire and black carbon addition.

Important findings The results showed that: (1) During the growing season, compared with CK, the α-diversity of ECM fungal community treated by fire decreased significantly by 31.52%, while it increased significantly by 49.02% under black carbon addition treatment. There were significant differences in the β-diversity of of ECM fungal community under fire and black carbon addition treatments during the growing season. (2) During the growing season, the abundance of ECM fungi treated by fire decreased significantly by 46.35%, but the fire treatment significantly increased Basidiomycetes species abundance, while black carbon addition treatment increased Ascomycetes and Basidiomycetes species abundance. (3) During the growing season, the composition of ECM fungal community in Larix gmelini forest was significantly affected by soil pH, water content and total nitrogen content under the fire and black carbon addition treatments, but it was positively correlated with soil pH and total nitrogen content and negatively correlated with soil water content in the black carbon addition treatment. The results of this study showed that fire reduced the diversity of ECM fungi in Larix gmelinii forest during the growing season, and significantly reduced the abundance of ECM fungi. However, the addition of black carbon after fire may change this negative effect. By affecting the changes of soil pH and soil nitrogen content, black carbon promoted the recovery of ECM fungi and increased its diversity. Therefore, the long-term impact of black carbon on ECM fungal community should be continuously monitored in the future.

Key words: fire, Larix gmelinii, ectomycorrhizal fungi, black carbon, diversity