Chin J Plant Ecol ›› 2020, Vol. 44 ›› Issue (7): 782-790.DOI: 10.17521/cjpe.2020.0114
• Research Articles • Previous Articles
PANG Fang1, XIA Wei-Kang1, HE Min1, QI Shan-Shan2, DAI Zhi-Cong1,2,*(), DU Dao-Lin1
Received:
2020-04-21
Accepted:
2020-06-12
Online:
2020-07-20
Published:
2020-06-23
Contact:
DAI Zhi-Cong: ORCID:0000-0002-0748-8059,daizhicong@163.com
Supported by:
PANG Fang, XIA Wei-Kang, HE Min, QI Shan-Shan, DAI Zhi-Cong, DU Dao-Lin. Nitrogen-fixing bacteria alleviates competition between arbuscular mycorrhizal fungi and Solidago canadensis for nutrients under nitrogen limitation[J]. Chin J Plant Ecol, 2020, 44(7): 782-790.
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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2020.0114
Fig. 1 Electrophoretogram of the isolated nitrogen-fixing bacterium. A, 16S rDNA. B, Nitrogen-fixing gene nifH. DL, DNA marker; NC, negative control; JS106, Paenibacillus stellifer ScRB-JS106.
Fig. 2 Neighbour-joining tree of the isolated nitrogen-fixing bacterium, ScRB-JS106, based on 16S rDNA. Sequences for the other isolates were downloaded from GenBank.
Fig. 3 Growth of Solidago canadensis under different concentrations of ammonium chloride (mean ± SE, n = 4). CK, negative control (10 mmol·L-1 N). Different lowercase letters indicate significant differences (Duncan’s test, p < 0.05).
Fig. 4 Growth of Solidago canadensis under different concentrations of potassium nitrate (mean ± SE, n = 4). CK, negative control (10 mmol·L-1 N). Different lowercase letters indicate significant differences (Duncan’s test, p < 0.05).
Fig. 5 Root colonization rate of Solidago canadensis inoculated with various arbuscular mycorrhizal fungi and nitrogen-fixing bacteria (mean ± SE, n = 6). A, Glomus etunicatum (GE). B, Glomus intraradices (GI). C, Glomus mosseae (GM). JS106, Paenibacillus stellifer ScRB-JS106. ns, no significant difference (p > 0.05).
Fig. 6 Growth of Solidago canadensis inoculated with Glomus etunicatum and nitrogen-fixing bacteria under nitrogen limitation (mean ± SE, n = 6). GE, G. etunicatum. JS106, Paenibacillus stellifer ScRB-JS106. CK, un-inoculated control. ns, no significant difference (p > 0.05).
Fig. 7 Growth of Solidago canadensis inoculated with Glomus intraradices and nitrogen-fixing bacteria (mean ± SE, n = 6). GI, Glomus intraradices. JS106, Paenibacillus stellifer ScRB-JS106. CK, un-inoculated control. ns, no significant difference (p > 0.05).
Fig. 8 Growth of Solidago canadensis inoculated with Glomus mosseae and nitrogen-fixing bacteria (mean ± SE, n = 6). GM, G. mosseae. JS106, Paenibacillus stellifer ScRB-JS106. CK, un-inoculated control. ns, no significant difference (p > 0.05).
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