Chin J Plant Ecol ›› 2023, Vol. 47 ›› Issue (2): 195-205.DOI: 10.17521/cjpe.2022.0004
Special Issue: 入侵生态学
• Research Articles • Previous Articles Next Articles
WANG Jing-Jing1, WANG Jia-Hao2, HUANG Zhi-Yun1, Vanessa Chiamaka OKECHUKW1, HU Die2, QI Shan-Shan2,*(), DAI Zhi-Cong1,3, DU Dao-Lin1
Received:
2022-01-05
Accepted:
2022-05-20
Online:
2023-02-20
Published:
2023-02-28
Contact:
*(Supported by:
WANG Jing-Jing, WANG Jia-Hao, HUANG Zhi-Yun, Vanessa Chiamaka OKECHUKW, HU Die, QI Shan-Shan, DAI Zhi-Cong, DU Dao-Lin. Effects of endophytic nitrogen-fixing bacteria on the growth strategy of an invasive plant Sphagneticola trilobata under different nitrogen levels[J]. Chin J Plant Ecol, 2023, 47(2): 195-205.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2022.0004
Fig. 1 Identification of nitrogen-fixing bacteria strain WTB-JS007. A, Colony morphology of WTB-JS007 on LB plate. B, Scanning electronic microscopy (SEM) of WTB-JS007. C, Phylogenetic tree of WTB-JS007 based on 16S rRNA gene sequence. D, Nitrogen fixation primer (F1-nifH-3r) PCR amplified gel electrophoresis. E, Phylogenetic tree of WTB-JS007 based on nitrogen fixation gene on the sequence amplified with nitrogen fixation primers. CK, control.
Fig. 2 Leaf area (A), stem length (B), number of roots (C) and root length (D) of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments of S. trilobata; different uppercase letters indicate significant differences (p < 0.05) among different treatments of S. calendulacea.
Fig. 3 Aboveground biomass (A), belowground biomass (B), specific leaf area (C) and root:shoot (D) of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments of S. trilobata; different uppercase letters indicate significant differences (p < 0.05) among different treatments of S. calendulacea.
Fig. 4 Nitrogen content of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments under the low-nitrogen level; different uppercase letters indicate significant differences (p < 0.05) among different treatments under the normal nitrogen level.
[1] |
Adomako MO, Xue W, Du DL, Yu FH (2022). Soil microbe- mediated N:P stoichiometric effects on Solidago canadensis performance depend on nutrient levels. Microbial Ecology, 83, 960-970.
DOI |
[2] |
Anas M, Liao F, Verma KK, Sarwar MA, Mahmood A, Chen ZL, Li Q, Zeng XP, Liu Y, Li YR (2020). Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency. Biological Research, 53, 47.
DOI PMID |
[3] |
Balasundararajan V, Dananjeyan B (2019). Occurrence of diversified N-acyl homoserine lactone mediated biofilm- forming bacteria in rice rhizoplane. Journal of Basic Microbiology, 59, 1031-1039.
DOI PMID |
[4] |
Bernacchi CJ, Coleman JS, Bazzaz FA, McConnaughay KDM (2000). Biomass allocation in old-field annual species grown in elevated CO2 environments: no evidence for optimal partitioning. Global Change Biology, 6, 855-863.
DOI URL |
[5] |
Chen L, Liang ZN, Zhu H (2015). Research advances in the studies of plant entophytic. Biotechnology Bulletin, 31, 30-34.
DOI |
[陈龙, 梁子宁, 朱华 (2015). 植物内生菌研究进展. 生物技术通报, 31, 30-34.]
DOI |
|
[6] |
Chen MY, Zhu B, Lin L, Yang LT, Li YR, An QL (2014). Complete genome sequence of Kosakonia sacchari type strain SP1T. Standards in Genomic Sciences, 9, 1311-1318.
DOI URL |
[7] |
Cocking EC (2003). Endophytic colonization of plant roots by nitrogen-fixing bacteria. Plant and Soil, 252, 169-175.
DOI URL |
[8] | Dai ZC, Du DL, Si CC, Lin Y, Hao JL, Sun F (2009). A method to exactly measure the morphological quantity of leaf using Scanner and Image J Software. Guihaia, 29, 342-347. |
[戴志聪, 杜道林, 司春灿, 林英, 郝建良, 孙凤 (2009). 用扫描仪及Image J软件精确测量叶片形态数量特征的方法. 广西植物, 29, 342-347.] | |
[9] |
Dai ZC, Fu W, Wan LY, Cai HH, Wang N, Qi SS, Du DL (2016). Different growth promoting effects of endophytic bacteria on invasive and native clonal plants. Frontiers in Plant Science, 7, 706. DOI: 10.3389/fpls.2016.00706.
DOI |
[10] |
Dai ZC, Wan LY, Qi SS, Rutherford S, Ren GQ, Wan JSH, Du DL (2020). Synergy among hypotheses in the invasion process of alien plants: a road map within a timeline. Perspectives in Plant Ecology, Evolution and Systematics, 47, 125575. DOI: 10.1016/j.ppees.2020.125575.
DOI |
[11] |
Dipta B, Bhardwaj S, Kaushal M, Kirti S, Sharma R (2019). Obliteration of phosphorus deficiency in plants by microbial interceded approach. Symbiosis, 78, 163-176.
DOI |
[12] |
Fedorov DN, Ivanova EG, Doronina NV, Trotsenko IA (2008). A new system of degenerate oligonucleotide primers for detection and amplification of nifHD genes. Mikrobiologiia, 77, 286-288.
PMID |
[13] |
Franche C, Lindström K, Elmerich C (2009). Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil, 321, 35-59.
DOI URL |
[14] |
Funk JL (2008). Differences in plasticity between invasive and native plants from a low resource environment. Journal of Ecology, 96, 1162-1173.
DOI URL |
[15] |
Funk JL (2013). The physiology of invasive plants in low-resource environments. Conservation Physiology, 1, cot026. DOI: 10.1093/conphys/cot026.
DOI |
[16] |
Hardoim PR, van Overbeek LS, van Elsas JD (2008). Properties of bacterial endophytes and their proposed role in plant growth. Trends in Microbiology, 16, 463-471.
DOI PMID |
[17] | Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular, 347, 23-32. |
[18] |
Javed Q, Sun JF, Azeem A, Jabran K, Du DL (2020). Competitive ability and plasticity of Wedelia trilobata (L.) under wetland hydrological variations. Scientific Reports, 10, 9431. DOI: 10.1038/s41598-020-66385-z.
DOI |
[19] |
Leishman MR, Haslehurst T, Ares A, Baruch Z (2007). Leaf trait relationships of native and invasive plants: community- and global-scale comparisons. New Phytologist, 176, 635-643.
DOI PMID |
[20] | Li H, Liao D, Su JQ, Huang FY, Hong YW (2014). Diversity and function of endophytic bacteria in roots of exotic plant Spartina alterniflora. Chinese Journal of Applied and Environmental Biology, 20, 856-862. |
[李虎, 廖丹, 苏建强, 黄福义, 洪有为 (2014). 外来种互花米草根内细菌多样性及功能. 应用与环境生物学报, 20, 856-862.] | |
[21] | Li Q, Chen Q, He FR, Bharani M, Dai ZC, Qi SS, Du DL (2020). Arbuscular mycorrhizal fungi promote the growth of Wedelia trilobata and the absorption of insoluble phosphorus. Journal of Tropical and Subtropical Botany, 28, 339-346. |
[李琴, 陈琪, 贺芙蓉, Bharani M, 戴志聪, 祁珊珊, 杜道林 (2020). 丛枝菌根真菌促进南美蟛蜞菊生长及对难溶磷的吸收. 热带亚热带植物学报, 28, 339-346.] | |
[22] |
Littschwager J, Lauerer M, Blagodatskaya E, Kuzyakov Y (2010). Nitrogen uptake and utilisation as a competition factor between invasive Duchesnea indica and native Fragaria vesca. Plant and Soil, 331, 105-114.
DOI URL |
[23] | Liu YT, Dai ZC, Xue YL, Sun JF, Zhu F, Du DL (2013). Prediction of suitable area of an alien invasive species (Wedelia trilobata) in China. Guangdong Agricultural Sciences, 40(14), 174-178. |
[刘勇涛, 戴志聪, 薛永来, 孙见凡, 朱方, 杜道林 (2013). 外来入侵植物南美蟛蜞菊在中国的适生区预测. 广东农业科学, 40(14), 174-178.] | |
[24] |
Matzek V (2011). Superior performance and nutrient-use efficiency of invasive plants over non-invasive congeners in a resource-limited environment. Biological Invasions, 13, 3005-3014.
DOI URL |
[25] |
Mei C, Flinn BS (2010). The use of beneficial microbial endophytes for plant biomass and stress tolerance improvement. Recent Patents on Biotechnology, 4, 81-95.
PMID |
[26] |
Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
DOI URL |
[27] |
Noori F, Etesami H, Najafi Zarini H, Khoshkholgh-Sima NA, Hosseini Salekdeh G, Alishahi F (2018). Mining alfalfa (Medicago sativa L.) nodules for salinity tolerant non- rhizobial bacteria to improve growth of alfalfa under salinity stress. Ecotoxicology and Environmental Safety, 162, 129-138.
DOI URL |
[28] |
Oldroyd GED, Leyser O (2020). A plantʼs diet, surviving in a variable nutrient environment. Science, 368, eaba0196. DOI: 10.1126/science.aba0196.
DOI |
[29] |
Paini DR, Sheppard AW, Cook DC, de Barro PJ, Worner SP, Thomas MB. (2016). Global threat to agriculture from invasive species. Proceedings of the National Academy of Sciences of the United States of America, 113, 7575-7579.
DOI PMID |
[30] |
Pang F, Xia WK, He M, Qi SS, Dai ZC, Du DL (2020). Nitrogen-fixing bacteria alleviates competition between arbuscular mycorrhizal fungi and Solidago canadensis for nutrients under nitrogen limitation. Chinese Journal of Plant Ecology, 44, 782-790.
DOI URL |
[庞芳, 夏维康, 何敏, 祁珊珊, 戴志聪, 杜道林 (2020). 固氮菌缓解氮限制环境中丛枝菌根真菌对加拿大一枝黄花的营养竞争. 植物生态学报, 44, 782-790.] | |
[31] |
Pyšek P, Hulme PE, Simberloff D, Bacher S, Blackburn TM, Carlton JT, Dawson W, Essl F, Foxcroft LC, Genovesi P, Jeschke JM, Kühn I, Liebhold AM, Mandrak NE, Meyerson LA, et al. (2020). Scientists’ warning on invasive alien species. Biological Reviews of the Cambridge Philosophical Society, 95, 1511-1534.
DOI URL |
[32] |
Qi SS, Dai ZC, Miao SL, Zhai DL, Si CC, Huang P, Wang RP, Du DL (2014). Light limitation and litter of an invasive clonal plant, Wedelia trilobata, inhibit its seedling recruitment. Annals of Botany, 114, 425-433.
DOI URL |
[33] | Qi SS, He FR, Wang JJ, Li Q, Dai ZC, Du DL (2020). Effects of arbuscular mycorrhizal fungi on the growth and the competition of an invasive plant Wedelia trilobata. Microbiology China, 47, 3801-3810. |
[祁珊珊, 贺芙蓉, 汪晶晶, 李琴, 戴志聪, 杜道林 (2020). 丛枝菌根真菌对入侵植物南美蟛蜞菊生长及竞争力的影响. 微生物学通报, 47, 3801-3810.] | |
[34] |
Ren GQ, He FR, Sun JF, Hu WJ, Azeem A, Qi SS, Yang B, Cui MM, Jiang K, Du DL (2021). Resource conservation strategy helps explain patterns of biological invasion in a low-N environment. Biochemical Systematics and Ecology, 94, 104295. DOI: 10.1016/j.bse.2020.104205.
DOI |
[35] |
Ren GQ, Li Q, Li Y, Li J, Adomako MO, Dai ZC, Li GL, Wan LY, Zhang B, Zou CB, Ran Q, Du DL (2019). The enhancement of root biomass increases the competitiveness of an invasive plant against a co-occurring native plant under elevated nitrogen deposition. Flora, 261, 151486. DOI: 10.1016/j.flora.2019.151486.
DOI |
[36] |
Richards CL, Bossdorf O, Muth NZ, Gurevitch J, Pigliucci M (2006). Jack of all trades, master of some? On the role of phenotypic plasticity in plant invasions. Ecology Letters, 9, 981-993.
PMID |
[37] |
Rosenblueth M, Martínez-Romero E (2006). Bacterial endophytes and their interactions with hosts. Molecular Plant-Microbe Interactions, 19, 827-837.
DOI PMID |
[38] |
Rout ME, Chrzanowski TH, Westlie TK, DeLuca TH, Callaway RM, Holben WE (2013). Bacterial endophytes enhance competition by invasive plants. American Journal of Botany, 100, 1726-1737.
DOI PMID |
[39] | Sambrook HC (1989). Molecular cloning : a laboratory manual. Cold Spring Harbor Perspectives in Biology, 10, 5-10. |
[40] |
Saravanan VS, Madhaiyan M, Osborne J, Thangaraju M, Sa TM (2008). Ecological occurrence of Gluconacetobacter diazotrophicus and nitrogen-fixing Acetobacteraceae members: their possible role in plant growth promotion. Microbial Ecology, 55, 130-140.
PMID |
[41] |
Sardans J, Peñuelas J (2012). The role of plants in the effects of global change on nutrient availability and stoichiometry in the plant-soil system. Plant Physiology, 160, 1741-1761.
DOI PMID |
[42] |
Wan FH, Guo JY, Wang DH (2002). Alien invasive species in China: their damages and management strategies. Biodiversity Science, 10, 119-125.
DOI URL |
[万方浩, 郭建英, 王德辉 (2002). 中国外来入侵生物的危害与管理对策. 生物多样性, 10, 119-125.]
DOI |
|
[43] | Wang MN, Dai ZC, Qi SS, Wang XY, Du DL (2014). Main hypotheses and research progress of alien plant invasion mechanism. Jiangsu Agricultural Sciences, 42, 378-382. |
[王明娜, 戴志聪, 祁珊珊, 王晓莹, 杜道林 (2014). 外来植物入侵机制主要假说及其研究进展. 江苏农业科学, 42, 378-382.] | |
[44] |
Wang YJ, Chen D, Yan R, Yu FH, van Kleunen M (2019). Invasive alien clonal plants are competitively superior over co-occurring native clonal plants. Perspectives in Plant Ecology, Evolution and Systematics, 40, 125484. DOI: 10.1016/j.ppees.2019.125484.
DOI |
[45] |
Wang YJ, Müller-Schärer H, van Kleunen M, Cai AM, Zhang P, Yan R, Dong BC, Yu FH (2017). Invasive alien plants benefit more from clonal integration in heterogeneous environments than natives. New Phytologist, 216, 1072-1078.
DOI URL |
[46] |
Wu YM, Leng ZR, Li J, Jia H, Yan CL, Hong HL, Wang Q, Lu YY, Du DL (2022). Increased fluctuation of sulfur alleviates cadmium toxicity and exacerbates the expansion of Spartina alterniflora in coastal wetlands. Environmental Pollution, 292, 118399. DOI: 10.1016/j.envpol.2021.118399.
DOI |
[47] | Wu YQ, Hu YJ, Liao FL (2005). Wedelia trilobata—A species from introduced to potential invasive. Guihaia, 25, 413-418. |
[吴彦琼, 胡玉佳, 廖富林 (2005). 从引进到潜在入侵的植物——南美蟛蜞菊. 广西植物, 25, 413-418.] | |
[48] |
Yu WB, Li SP (2020). Modern coexistence theory as a framework for invasion ecology. Biodiversity Science, 28, 1362-1375.
DOI URL |
[1] | Wei-Wei SHU kun YANG Jun-xu MA huilin MIN lin CHEN Liu ShiLing riyi HUANG Cai-dao MING zuwei TIAN. Effects of nitrogen addition on the morphological and chemical characters of fine roots different order of Castanopsis hystrix [J]. Chin J Plant Ecol, 2024, 48(预发表): 0-0. |
[2] | Xue-Qi GENG YA-KUN TANG WANG LiNa Xu DENG Ze-ling ZHANG Ying ZHOU. Nitrogen addition increases the biomass of Chinese terrestrial plants but reduces their Nitrogen use efficiency [J]. Chin J Plant Ecol, 2024, 48(预发表): 0-0. |
[3] | ZHANG Ying, ZHANG Chang-Hong, WANG Qi-Tong, ZHU Xiao-Min, YIN Hua-Jun. Difference of soil carbon sequestration between rhizosphere and bulk soil in a mountain coniferous forest in southwestern China under nitrogen deposition [J]. Chin J Plant Ecol, 2023, 47(9): 1234-1244. |
[4] | ZHAO Yan-Chao, CHEN Li-Tong. Soil nutrients modulate response of aboveground biomass to warming in alpine grassland on the Qingzang Plateau [J]. Chin J Plant Ecol, 2023, 47(8): 1071-1081. |
[5] | SU Wei, CHEN Ping, WU Ting, LIU Yue, SONG Yu-Ting, LIU Xu-Jun, LIU Ju-Xiu. Effects of nitrogen addition and extended dry season on non-structural carbohydrates, nutrients and biomass of Dalbergia odorifera seedlings [J]. Chin J Plant Ecol, 2023, 47(8): 1094-1104. |
[6] | LI Hong-Qin, ZHANG Fa-Wei, YI Lü-Bei. Stoichiometric responses in topsoil and leaf of dominant species to precipitation change and nitrogen addition in an alpine meadow [J]. Chin J Plant Ecol, 2023, 47(7): 922-931. |
[7] | ZHONG Qi, LI Zeng-Yan, MA Wei, KUANG Yu-Xiao, QIU Ling-Jun, LI Yun-Jie, TU Li-Hua. Effects of nitrogen addition and litter manipulations on leaf litter decomposition in western edge of Sichuan Basin, China [J]. Chin J Plant Ecol, 2023, 47(5): 629-643. |
[8] | ZHANG Ya-Qi, PANG Dan-Bo, CHEN Lin, CAO Meng-Hao, HE Wen-Qiang, LI Xue-Bin. Response of ammonia oxidizing bacteria to nitrogen fertilization and plant litter input on desert steppe [J]. Chin J Plant Ecol, 2023, 47(5): 699-712. |
[9] | LI Xiao-Ling, ZHU Dao-Ming, YU Yu-Rong, WU Hao, MOU Li, HONG Liu, LIU Xue- Fei, BU Gui-Jun, XUE Dan, WU Lin. Effects of simulated nitrogen deposition on growth and decomposition of two bryophytes in ombrotrophic peatland, southwestern Hubei, China [J]. Chin J Plant Ecol, 2023, 47(5): 644-659. |
[10] | LI Hui-Xuan, MA Hong-Liang, YIN Yun-Feng, GAO Ren. Dynamic of labile, recalcitrant carbon and nitrogen during the litter decomposition in a subtropical natural broadleaf forest [J]. Chin J Plant Ecol, 2023, 47(5): 618-628. |
[11] | ZHANG Jin-Yan, CUN Zhu, SHUANG Sheng-Pu, HONG Jie, MENG Zhen-Gui, CHEN Jun-Wen. Steady-state and dynamic photosynthetic characteristics of shade-tolerant species Panax notoginseng in response to nitrogen levels [J]. Chin J Plant Ecol, 2023, 47(3): 331-347. |
[12] | DU Ying-Dong, YUAN Xiang-Yang, FENG Zhao-Zhong. Effects of different nitrogen forms on photosynthesis characteristics and growth of poplar [J]. Chin J Plant Ecol, 2023, 47(3): 348-360. |
[13] | LUO Lai-Cong, LAI Xiao-Qin, BAI Jian, LI Ai-Xin, FANG Hai-Fu, Nasir SHAD, TANG Ming, HU Dong-Nan, ZHANG Ling. Effects of soil bacteria and fungi on growth of invasive plant Triadica sebifera with different provenances under nitrogen addition [J]. Chin J Plant Ecol, 2023, 47(2): 206-215. |
[14] | Li Baoyin Quan-Lin ZHONG Dong-Liang CHENG Chao-Bin XU Zou Yuxing Zhang Xue Lin Qiuyan Deng Xingyu. Growth and functional traits of Machilus pauhoi seedlings from different provenances in response to nitrogen addition [J]. Chin J Plant Ecol, 2023, 47(12): 1693-1707. |
[15] | FENG Xu-Fei, LEI Zhang-Ying, ZHANG Yu-Jie, XIANG Dao, YANG Ming-Feng, ZHANG Wang-Feng, ZHANG Ya-Li. Effect of leaf nitrogen allocation on photosynthetic nitrogen use efficiency at flowering and boll stage of Gossypium spp. [J]. Chin J Plant Ecol, 2023, 47(11): 1600-1610. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2022 Chinese Journal of Plant Ecology
Tel: 010-62836134, 62836138, E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn