Effects of nitrogen addition and extended dry season on non-structural carbohydrates, nutrients and biomass of Dalbergia odorifera seedlings

doi:10.17521/cjpe.2022.0473

Abstract

Abstract:

Aims Nitrogen addition and extended dry season can profoundly affect non-structural carbohydrates (NSCs), carbon (C), nitrogen (N) and phosphorus (P) concentrations, and biomass of plants, however, the different responses of various organs (leaf, trunk, stem and root) in the same plants are not clear. We thus explored such responses to N addition and extended dry season to help better predict plant growth under global changes.

Methods The investigation was conducted with Dalbergia odorifera seedlings through a manipulated N and water experiment. The concentrations of NSCs and C, N, and P and allocation pattern of biomass in different organs of D. odorifera seedling were determined, and then compared among different treatments.

Important findings (1) N addition significantly increased trunk NSCs concentrations, while the interaction of N addition and extended dry season significantly decreased NSCs concentrations. N addition, extended dry season and their interaction significantly increased root soluble sugar concentrations. (2) N addition significantly increased root N concentrations and decreased C:N, and the interaction of N addition and extended dry season significantly decreased C:P and N:P in trunks. (3) All treatments did not influence the total biomass of D. odorifera, but significantly decreased the ratio of leaf biomass to total biomass, and N addition and extended dry season significantly decreased the ratio of root biomass to shoot biomass but increased the ratio of stem biomass to leaf biomass. Overall, N addition may promote the growth of seedling trunks and improve the economic value of D. odoriferaunder the extended dry season in the future. In order to prevent the growth of D. odorifera from being inhibited under drought stress, it is necessary to replenish an appropriate amount of water in the dry season.

Key words: nitrogen addition, extended dry season, non-structural carbohydrate, nutrient, biomass, Dalbergia odorifera

SU Wei, CHEN Ping, WU Ting, LIU Yue, SONG Yu-Ting, LIU Xu-Jun, LIU Ju-Xiu. Effects of nitrogen addition and extended dry season on non-structural carbohydrates, nutrients and biomass of Dalbergia odorifera seedlings[J]. Chin J Plant Ecol, 2023, 47(8): 1094-1104.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2022.0473

https://www.plant-ecology.com/EN/Y2023/V47/I8/1094

Figures/Tables 6

Fig. 1 Basic information of the sample plots for nitrogen-water interaction experiment in South China Botanical Garden. A, Four treatments. CK, control; ED, extended dry season; MN, nitrogen addition; MNED, nitrogen addition × extended dry season. B, In situ experimental photograph.

Table 1 Allometric growth equations and related statistical parameters for different organs of Dalbergia odorifera

器官 Organ	方程 Equation	F	R²	p
叶 Leaf	B = 0.586 × (D²H)^0.904	7.166	0.230	0.013
树干 Trunk	B = 0.858 × (D²H)^0.617	65.402	0.723	<0.001
枝 Stem	B = 0.557 × (D²H)^2.814	6.767	0.244	0.017
根 Root	B = 0.654 × (D²H)^1.317	27.893	0.538	<0.001
总计 Total	B = 0.662 × (D²H)^5.534	46.929	0.662	<0.001

Fig. 2 Variations in non-structural carbohydrate concentrations and ratios in different organs of Dalbergia odorifera under different treatments (mean ± SE, n = 3). CK, control; ED, extended dry season; MN, nitrogen addition; MNED, nitrogen addition × extended dry season. Different lowercase letters indicate significant differences between treatments (p < 0.05).

Fig. 3 Variation in nutrient concentrations and ratios in different organs of Dalbergia odorifera under different treatments (mean ± SE, n = 3). CK, control; ED, extended dry season; MN, nitrogen addition; MNED, nitrogen addition × extended dry season. Different lowercase letters indicate significant differences between treatments (p < 0.05).

Table 2 Variation in the biomass allocation among different organs of Dalbergia odorifera under different treatments (mean ± SE, n = 3)

	处理 Treatment	叶 Leaf	树干 Trunk	枝 Stem	根 Root	总计 Total
鲜质量 Fresh mass (kg)	CK	1.21 ± 0.08^a (12.80%^a)	5.83 ± 0.41^a (39.72%^b)	2.04 ± 0.25^a (23.42%^a)	2.04 ± 0.19^a (24.06%^a)	8.22 ± 0.36^a
	ED	0.52 ± 0.14^c (15.05%^a)	4.41 ± 1.01^a (58.46%^a)	1.07 ± 0.15^c (13.33%^a)	1.24 ± 0.25^b (13.16%^b)	4.37 ± 1.03^b
	MN	0.53 ± 0.08^c (12.66%^a)	5.46 ± 1.44^a (49.25%^ab)	1.27 ± 0.12^bc (26.89%^a)	1.34 ± 0.15^b (11.20%^b)	5.83 ± 1.02^ab
	MNED	0.90 ± 0.46^b (15.21%^a)	5.77 ± 0.48^a (44.61%^ab)	1.41 ± 0.04^b (22.74%^a)	2.22 ± 0.21^a (17.44%^ab)	8.36 ± 1.09^a
干质量 Dry mass (kg)	CK	0.45 ± 0.06^a (7.89%^a)	3.25 ± 0.31^a (56.37%^a)	1.01 ± 0.20^a (17.48%^a)	1.06 ± 0.10^ab (18.27%^ab)	5.77 ± 0.18^a
	ED	0.19 ± 0.05^c (4.83%^b)	2.54 ± 1.02^a (63.24%^a)	0.55 ± 0.13^b (15.74%^a)	0.63 ± 0.12^c (16.19%^b)	3.92 ± 0.66^a
	MN	0.20 ± 0.04^c (4.73%^b)	2.94 ± 1.29^a (63.92%^a)	0.61 ± 0.06^b (14.45%^a)	0.83 ± 0.12^bc (16.91%^ab)	4.48 ± 0.87^a
	MNED	0.31 ± 0.04^b (5.48%^b)	3.14 ± 0.21^a (61.66%^a)	0.64 ± 0.04^b (11.23%^a)	1.52 ± 0.145^a (21.64%^a)	5.83 ± 0.59^a

Fig. 4 Root shoot ratio (R:S) and stem leaf ratio (S:L) in Dalbergia odorifera (mean ± SE, n = 3). CK, control; ED, extended dry season; MN, nitrogen addition; MNED, nitrogen addition × extended dry season. Different lowercase letters indicate significant differences between treatments (p < 0.05).

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