Interaction of soil arbuscular mycorrhizal fungi and plant roots acts on maintaining soil phosphorus availability under nitrogen addition

doi:10.17521/cjpe.2021.0280

Abstract

Abstract:

Aims Phosphorus is one of the major limiting nutrients for plant growth in subtropical areas, whereas increasing nitrogen deposition may be a limiting factor in determining the availability of soil phosphorus. Here, focusing on soil microorganisms and plant fine roots, we explored the transformation of soil phosphorus to unravel the maintenance of soil phosphorus supply and plant productivity with low availability under nitrogen deposition.

Methods At the Fuzhou Changʼan Mountain in Fujian Province, China, control (0 kg·hm^-2·a^-1), low nitrogen (40 kg·hm^-2·a^-1), and high nitrogen (80 kg·hm^-2·a^-1) treatments were set up to simulate nitrogen addition. Soil and root samples of Cunninghamia lanceolata seedlings were then collected to comprehensively analyze soil phosphorus and nutrient contents as well as microbiological-plant root characteristics.

Important findings The results showed that the contents of soil labile organic phosphorus, moderately labile inorganic phosphorus and occluded phosphorus were significantly increased, whereas those of primary mineral phosphorus and moderately labile organic phosphorus decreased under the low nitrogen treatment as compared to the control treatment. However, there were no significant changes under the high nitrogen treatment. Redundancy analysis indicated that soil acid phosphatase activity, relative abundance of mycorrhizal fungi, soil microbial biomass phosphorus content, and root biomass were important soil microbiological-plant root characteristics factors that could explain the changes in soil phosphorus components. Variance partitioning analysis revealed that the soil microbiological-plant root characteristics synergy explained 57% of the alternations in soil phosphorus components, whereas correlation analysis showed a significant positive correlation between the relative abundance of mycorrhizal fungi and root biomass. Overall, these results suggest that mycorrhizal colonization is promoted under a low level of nitrogen input and the synergistic action of mycorrhizal fungi and C. lanceolata fine roots promotes the conversion of moderately labile organic and primary mineral phosphorus to labile phosphorus, thus maintaining the growth of C. lanceolata seedlings.

Key words: nitrogen addition, phosphorus, microbiologic characteristic, root characteristic, Cunninghamia lanceolata, seedling

XIE Huan, ZHANG Qiu-Fang, CHEN Ting-Ting, ZENG Quan-Xin, ZHOU Jia-Cong, WU Yue, LIN Hui-Ying, LIU Yuan-Yuan, YIN Yun-Feng, CHEN Yue-Min. Interaction of soil arbuscular mycorrhizal fungi and plant roots acts on maintaining soil phosphorus availability under nitrogen addition[J]. Chin J Plant Ecol, 2022, 46(7): 811-822.

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Figures/Tables 11

References 44

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处理 Treatment	pH	总碳含量 Total carbon (C) content (g·kg^-1)	总氮含量 Total N content (g·kg^-1)	有效氮含量 Available N content (mg·kg^-1)	可溶性有机碳含量 Dissolved organic C content (mg·kg^-1)	可溶性有机氮含量 Dissolved organic N content (mg·kg^-1)
CK	4.55 ± 0.06^a	16.30 ± 0.23^a	1.47 ± 0.03^a	7.97 ± 0.22^b	7.67 ± 0.60^a	4.61 ± 0.40^b
LN	4.51 ± 0.06^a	16.20 ± 0.04^a	1.48 ± 0.05^a	8.58 ± 0.49^a	6.78 ± 0.44^b	6.68 ± 0.36^a
HN	4.40 ± 0.09^b	16.61 ± 0.24^a	1.50 ± 0.02^a	8.83 ± 0.25^a	3.99 ± 0.71^c	7.24 ± 0.71^a
p	0.03	0.06	0.57	0.01	<0.01	<0.01

处理 Treatment	pH	总碳含量 Total carbon (C) content (g·kg^-1)	总氮含量 Total N content (g·kg^-1)	有效氮含量 Available N content (mg·kg^-1)	可溶性有机碳含量 Dissolved organic C content (mg·kg^-1)	可溶性有机氮含量 Dissolved organic N content (mg·kg^-1)
CK	4.55 ± 0.06^a	16.30 ± 0.23^a	1.47 ± 0.03^a	7.97 ± 0.22^b	7.67 ± 0.60^a	4.61 ± 0.40^b
LN	4.51 ± 0.06^a	16.20 ± 0.04^a	1.48 ± 0.05^a	8.58 ± 0.49^a	6.78 ± 0.44^b	6.68 ± 0.36^a
HN	4.40 ± 0.09^b	16.61 ± 0.24^a	1.50 ± 0.02^a	8.83 ± 0.25^a	3.99 ± 0.71^c	7.24 ± 0.71^a
p	0.03	0.06	0.57	0.01	<0.01	<0.01

处理 Treatment	子囊菌门 Ascomycota	担子菌门 Basidiomycotaota	被孢菌门 Mortierellomycota	未定义 Unclassified	罗兹菌门 Rozellomycota	球囊菌门 Glomeromycota	其他 Other
CK	38.73 ± 9.28^a	26.04 ± 7.02^a	11.94 ± 3.16^a	11.18 ± 1.03^a	9.87 ± 6.22^a	0.27 ± 0.01^b	1.97 ± 0.56^a
LN	39.02 ± 6.00^a	26.16 ± 3.33^a	16.86 ± 3.52^a	10.83 ± 4.13^a	4.61 ± 0.97^a	1.09 ± 0.11^a	1.43 ± 0.48^a
HN	35.05 ± 12.21^a	37.24 ± 13.12^a	14.43 ± 2.39^a	8.11 ± 3.91^a	3.77 ± 1.27^a	0.62 ± 0.01^a	0.90 ± 0.63^a
p	0.809	0.175	0.129	0.400	0.091	<0.001	0.072

处理 Treatment	子囊菌门 Ascomycota	担子菌门 Basidiomycotaota	被孢菌门 Mortierellomycota	未定义 Unclassified	罗兹菌门 Rozellomycota	球囊菌门 Glomeromycota	其他 Other
CK	38.73 ± 9.28^a	26.04 ± 7.02^a	11.94 ± 3.16^a	11.18 ± 1.03^a	9.87 ± 6.22^a	0.27 ± 0.01^b	1.97 ± 0.56^a
LN	39.02 ± 6.00^a	26.16 ± 3.33^a	16.86 ± 3.52^a	10.83 ± 4.13^a	4.61 ± 0.97^a	1.09 ± 0.11^a	1.43 ± 0.48^a
HN	35.05 ± 12.21^a	37.24 ± 13.12^a	14.43 ± 2.39^a	8.11 ± 3.91^a	3.77 ± 1.27^a	0.62 ± 0.01^a	0.90 ± 0.63^a
p	0.809	0.175	0.129	0.400	0.091	<0.001	0.072

处理 Treatment	酸性磷酸单酯酶 Acid phosphomonoesterase (nmol·g^-1·h^-1)	酸性磷酸双酯酶 Acid phosphodiesterase (nmol·g^-1·h^-1)	微生物生物量碳含量 Microbial biomass carbon content (mg·kg^-1)	微生物生物量氮含量 Microbial biomass N content (mg·kg^-1)	微生物生物量磷含量 Microbial biomass phosphorus content (mg·kg^-1)
CK	24.68 ± 2.44^b	1.67 ± 0.08^a	215.42 ± 21.87^c	29.59 ± 1.40^a	41.07 ± 3.31^a
LN	48.89 ± 4.08^a	1.72 ± 0.05^a	273.59 ± 18.53^b	26.45 ± 0.98^b	21.75 ± 2.09^b
HN	19.59 ± 2.90^c	1.19 ± 0.03^b	358.27 ± 20.05^a	27.62 ± 0.95^b	20.78 ± 6.45^b
p	<0.001	<0.001	<0.001	0.003	<0.001