除草剂苄嘧磺隆对粗梗水蕨配子体生长和性器官分化的影响

doi:10.3773/j.issn.1005-264x.2008.02.019

植物生态学报 ›› 2008, Vol. 32 ›› Issue (2): 408-412.DOI: 10.3773/j.issn.1005-264x.2008.02.019

除草剂苄嘧磺隆对粗梗水蕨配子体生长和性器官分化的影响

陶玲¹^,²(), 尹黎燕¹, 李伟¹^,^*()

1 中国科学院武汉植物园水生植物生物学实验室,武汉 430074
2 中国科学院研究生院,北京 100049

收稿日期:2007-03-22 接受日期:2007-08-02 出版日期:2008-03-22 发布日期:2008-03-30
通讯作者: 陶玲,李伟
作者简介:E-mail: tling04@mails.gucas.ac.cn
* E-mail: liwei@rose.whiob.ac.cn;
基金资助:
国家高技术研究发展计划项目(2005AA6010100503);国家自然科学基金委员会项目(30570291);国家重点基础研究发展规划项目(2002CB412300)

EFFECTS OF HERBICIDE BENSULFURON-METHYL ON GAMETOPHYTE DEVELOPMENT AND SEX ORGAN DIFFERENTIATION IN CERATOPTERIS PTERIDOIDES

TAO Ling¹^,²(), YIN Li-Yan¹, LI Wei¹^,^*()

¹Laboratory of Aquatic Plant Biology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
²Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2007-03-22 Accepted:2007-08-02 Online:2008-03-22 Published:2008-03-30
Contact: TAO Ling,LI Wei

摘要/Abstract

摘要：

稻田施用的除草剂可能会污染邻近水域,对非靶标水生植物产生影响。国家二级重点保护野生植物粗梗水蕨(Ceratopteris pteridoides)主要分布于稻田及其周围水域,其有性繁殖敏感阶段与除草剂施用时间一致,配子体易暴露在除草剂污染中。为了评价除草剂污染对水生态环境特别是对非靶标国家重点保护野生水生植物的影响,保护生物多样性和水生生态环境,该文研究了除草剂苄嘧磺隆(Bensulfuron-methyl)对粗梗水蕨配子体生长和性器官分化的影响。结果表明,苄嘧磺隆对粗梗水蕨孢子萌发没有影响,但对其配子体生长有抑制作用。苄嘧磺隆浓度和粗梗水蕨配子体面积呈明显的剂量-效应关系,苄嘧磺隆对粗梗水蕨配子体生长抑制的EC₅₀(半数效应浓度)为0.086 μg·L^-1,低于已报道的环境背景浓度。随着苄嘧磺隆浓度升高,粗梗水蕨的雌雄同体配子体逐渐减少,且配子体上颈卵器形成时间推迟。在苄嘧磺隆为10 μg·L^-1时,粗梗水蕨没有雌雄同体配子体形成。以上研究结果表明,低浓度苄嘧磺隆可抑制粗梗水蕨配子体的生长并影响其性器官的分化,使粗梗水蕨有性繁殖能力减弱。

关键词: 除草剂, 苄嘧磺隆, 粗梗水蕨, 有性繁殖

Abstract:

Aims Herbicides applied to rice fields may contaminate nearby aquatic systems through a variety of mechanisms, including drift, surface runoff and leaching, and may have adverse effects on non-target aquatic plants that play a major role in the aquatic ecosystem. The second category of the key protected wild plant, Ceratopteris pteridoides, is frequently observed in paddy fields and adjacent lakes and ponds. Its sexual reproduction phase coincides with herbicide application; therefore, its gametophytes are likely exposed to rice herbicides. The objective of this study is to assess the ecological hazard of herbicide contamination on this aquatic plant.
Methods We cultured spores of C. pteridoides in aqueous solutions of different bensulfuron-methyl concentrations and observed gametophyte growth and sex organ differentiation.
Important findings Bensulfuron-methyl had no effect on spore germination of C. pteridoides. However, it inhibited gametophyte growth with the EC₅₀ of 0.086 μg·L^-1, which was below the reported environment concentration. Bensulfuron-methyl reduced the ratio of hermaphrodite gametophyte and delayed archegonium differentiation of C. pteridoides with the increase in concentration. When bensulfuron-methyl concentration was 10 μg·L^-1, gametophytes ceased growth and did not form archegonia. Therefore, bensulfuron-methyl inhibits gametophyte growth and sex organ differentiation of C. pteridoides at low concentration and may pose a risk to sexual reproduction of C. pteridoides in the field.

Key words: herbicide, bensulfuron-methyl, Ceratopteris pteridoides, sexual reproduction

陶玲, 尹黎燕, 李伟. 除草剂苄嘧磺隆对粗梗水蕨配子体生长和性器官分化的影响. 植物生态学报, 2008, 32(2): 408-412. DOI: 10.3773/j.issn.1005-264x.2008.02.019

TAO Ling, YIN Li-Yan, LI Wei. EFFECTS OF HERBICIDE BENSULFURON-METHYL ON GAMETOPHYTE DEVELOPMENT AND SEX ORGAN DIFFERENTIATION IN CERATOPTERIS PTERIDOIDES. Chinese Journal of Plant Ecology, 2008, 32(2): 408-412. DOI: 10.3773/j.issn.1005-264x.2008.02.019

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.02.019

https://www.plant-ecology.com/CN/Y2008/V32/I2/408

图/表 4

图1 苄嘧磺隆对粗梗水蕨孢子萌发的影响数据为平均值±标准偏差(n=3)

Fig.1 The effect of bensulfuron-methyl on spore germination of Ceratopteris pteridoides Data are means±SD (n=3)

图2 苄嘧磺隆对粗梗水蕨配子体生长的影响数据为平均值±标准偏差(n=3)

Fig.2 The effect of bensufuron-methyl on gametophyte growth of Ceratopteris pteridoides Data are means±SD (n=3)

图3 苄嘧磺隆对粗梗水蕨配子体性器官分化的影响数据为平均值±标准偏差(n=3)

Fig.3 The effect of bensufuron-methyl on sex organ differentiation of Ceratopteris pteridoides gametophyte Data are means±SD (n=3)

表1 苄嘧磺隆对粗梗水蕨配子体上颈卵器形成的影响

Table 1 The effect of bensufuron methyl on archegonium development of Ceratopteris pteridoides

苄嘧磺隆浓度 Bensulfuron-methyl concentration (μg·L^-1)	颈卵器出现时间 Time of archegonium emergence (d)	颈卵器数量 Archegonium number
0	7	4~5
0.01	8	4~5
0.1	9	4~5
1	15	3~4
5	20	1
10	-	0

参考文献 22

[1]	Aida M, Ikedab H, Itohc K, Usui K (2006). Effects of five rice herbicides on the growth of two threatened aquatic ferns. Ecotoxicology and Environmental Safety, 63, 463-468.
[2]	Aida M, Itoh K, Ikeda H, Harada N, Ishii Y, Usui K (2004). Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl. Weed Biology and Management, 4, 127-135.
[3]	Banks JA, Hickok L, Webb MA (1993). The programming of sexual phenotype in the homosporous fern Ceratopteris richardii. International Journal of Plant Sciences, 154, 522-534.
[4]	Chasan R (1992). Ceratopteris: a model plant for the 90s. Plant Cell, 4, 113-115.
[5]	Davies J, Honegger LJ, Tencalla FG, Meregalli G, Brain PR, Newman RJ, Pitchford FH (2003). Herbicide risk assessment for non-target aquatic plants: sulfosulfuron—a case study. Pest Management Science, 59, 231-237.
[6]	Environment Agency of Japan (2000). Threatened Wildlife of Japan—Red Data Book 2nd edn. Japan Wildlife Research Center, Tokyo. (in Japanese)
[7]	Knauer K, Vervliet-Scheebaum M, Dark RJ, Maund SJ (2006). Methods for assessing the toxicity of herbicides to submersed aquatic plants. Pest Management Science, 62, 715-722. URL PMID
[8]	Liang LN (梁丽娜), Guo PY (郭平毅), Li QF (李奇峰) (2006). The situation, problems and prospect of herbicides industry in China. Chinese Agriculture Information (中国农业信息), (2), 8-9. (in Chinese)
[9]	Lin CF (林长福) (2000). The application and development of bensulfuron-methyl in China. Pesticide (农药), 39(3), 11-12. (in Chinese)
[10]	Luo XY, Ikeda H (2005). Effects of four rice herbicides on seed germination and seedling growth of a threatened vascular plant Penthorum chinense Pursh. Bulletin of Environmental Contamimation and Toxicology, 75, 382-389.
[11]	Obrigawitch TT, Cook G, Wetherington J (1998). Assessment of effects on non-target plants from sulfonylurea herbicides using field approaches. Pesticide Science, 52, 199-217.
[12]	Roshon RD, McCann JH, Thompson DG, Stephenson GR (1999). Effects of seven forestry management herbicides on Myriophyllum sibiricum, as compared with other non-target aquatic organisms. Canadian Journal of Forest Research, 29, 1158-1169.
[13]	Sabater C, Cuesta A, Carrasco R (2002). Effects of bensulfuron-methyl and cinosulfuron on growth of four freshwater species of phytoplankton. Chemosphere, 46, 953-960.
[14]	Seefeldt SS, Jensen EJ, Patrick FE (1995). Log-logistic analysis of herbicide dose-response relationships. Weed Technology, 9, 218-227.
[15]	Ueji M, Inao K (2001). Rice paddy field herbicides and their effects on the environment and ecosystems. Weed Biology and Management, 1, 71-79.
[16]	Warne TR, Hickok LG (1987). (2-Chloroethyl) phosphonic acid promotes germination of immature spores of Ceratopteris richardii Brongn. Plant Physiology, 83, 723-725.
[17]	Wei DB (魏东斌), Zhang AQ (张爱茜), Han SK (韩朔睽), Wang LS (王连生) (1999). The advances in sulfonylurea herbicides. Advances in Environmental Science (环境科学进展), 7(5), 34-42. (in Chinese)
[18]	Wu XX (吴晓霞), Wu JC (吴进才), Jin YG (金银根), Dong B (董波), Wang RS (王荣生) (2004). Impact of herbicides on physiology and ecology of aquatic plants. Acta Ecologica Sinica (生态学报), 24, 2037-2042. (in Chinese with English abstract)
[19]	Wu ZH (吴兆洪), Qin RC (秦仁昌) (1991). Fern Families and Genera of China (中国蕨类植物科属志). Science Press, Beijing. (in Chinese)
[20]	Yu YF (于永福) (1999). A mile stone of wild plant conservation in China. Plants (植物杂志), (5), 3. (in Chinese)
[21]	Yue XL (岳霞丽), Zhang XP (张新萍), Hu XW (胡先文), Dong YY (董元彦) (2006). Effect of bensulfuron-methyl on growth of Chlorella pyrenoidosa. Scientia Agricultura Sinica (中国农业科学), 39, 1823-1827. (in Chinese with English abstract)
[22]	Zhang XP (张新萍), Yue XL (岳霞丽), Hu XW (胡先文), Dong YY (董元彦) (2005). Effect of bensulfuron-methyl on the growth of Anabaena flos-aquae. Guangxi Agriculture Sciences (广西农业科学), 36, 251-252. (in Chinese with English abstract)

除草剂苄嘧磺隆对粗梗水蕨配子体生长和性器官分化的影响

EFFECTS OF HERBICIDE BENSULFURON-METHYL ON GAMETOPHYTE DEVELOPMENT AND SEX ORGAN DIFFERENTIATION IN CERATOPTERIS PTERIDOIDES

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