具混合繁殖策略的草本植物异果舞花姜的居群遗传结构

doi:10.3773/j.issn.1005-264x.2008.04.003

摘要/Abstract

摘要：

居群遗传结构的形成受到各种因素的影响。其中, 繁殖方式可能对居群内遗传变异有极其重要的意义, 而距离隔离也是居群间变异产生的主要原因之一。异果舞花姜(Globba racemosa)具有混合繁殖策略(以种子进行有性繁殖和以珠芽进行无性克隆繁殖)。调查分布于云南的7个异果舞花姜居群间有性与无性克隆繁殖的差异。采用ISSR标记研究各个居群的遗传多样性与克隆多样性, 探讨繁殖方式和距离隔离对居群遗传结构的影响。调查结果表明, 异果舞花姜各个居群存在一定的繁殖差异。ISSR结果显示, 该种在种水平上呈现较高水平的遗传变异(PPB=71.19%), 大部分的变异来自于居群间(G_ST = 0.590 7)。同时, 异果舞花姜具有较高水平克隆多样性(G/N = 0.88)。遗传多样性和克隆多样性与繁殖水平的变异间相关性不明显, 说明繁殖方式不是居群遗传结构形成的必要和决定性的因素。居群间的地理距离与遗传距离显著相关(r = 0.68, p < 0.05), 表明距离隔离是居群间遗传变异形成的重要原因。其它因素(如少量新有性个体的补充、细胞突变、奠基效应等)也对异果舞花姜居群遗传结构的形成和维持起到了重要作用。

关键词: 异果舞花姜, 有性繁殖, 克隆繁殖ISSR, 遗传多样性, 克隆多样性

Abstract:

Aims Our objective was to understand the relative effects of reproductive mode, geographic distance and other potential factors on within- and among-population genetic and clonal diversity in Globba racemosa, a species that can reproduce sexually (via seeds) and asexually (via bulbils).

Methods We investigated reproduction variation in the seven natural G. racemosa populations in Yuannan Province, China by measuring the number of sexually produced fruits and asexually produced bulbils. Genetic data were collected using inter-simple sequence repeat (ISSR) markers. We evaluated the distribution of genetic variance and levels of population genetic diversity and assessed the relationship between genetic diversity and reproduction variation.

Important findings Globba racemosa has a relatively high genetic diversity at the species level (PPB=71.19%), and most genetic variances resided among populations (G_ST = 0.590 7). A high clonal diversity was found in this species (G/N = 0.88). No significant difference was detected between reproductive mode and genetic diversity or clonal diversity, which suggested that the mode of reproduction was not necessarily a determinant of population genetic diversity. There was a significant correlation between genetic and geographical distances (r = 0.68, p < 0.05), which indicated that genetic differentiation between populations was likely attributed to the effect of isolation by distance. Other factors (e.g., small sexual recruitment, somatic mutation and founder effect, etc.) could also play important roles in maintaining genetic and clonal diversity in G. racemosa.

Key words: genetic diversity, clonal diversity, Globba racemosa, ISSR, mantel test, sexual vs. clonal reproduction

周会平, 陈进, 张寿洲. 具混合繁殖策略的草本植物异果舞花姜的居群遗传结构. 植物生态学报, 2008, 32(4): 751-759. DOI: 10.3773/j.issn.1005-264x.2008.04.003

ZHOU Hui-Ping, CHEN Jin, ZHANG Shou-Zhou. GENETIC AND CLONAL DIVERSITY OF GLOBBA RACEMOSA, A HERB WITH A MIXED REPRODUCTIVE MODE. Chinese Journal of Plant Ecology, 2008, 32(4): 751-759. DOI: 10.3773/j.issn.1005-264x.2008.04.003

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https://www.plant-ecology.com/CN/Y2008/V32/I4/751

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	Pop1	Pop2	Pop3	Pop4	Pop5	Pop6	Pop7
Latitude/longitude	21o53′ N/ 100o58′ E	22o53′ N/ 99o48′ E	24o04′ N/ 97o48′ E	24o05′ N/ 99o44′ E	24o25′ N/ 97o50′ E	24o35′ N/ 97o40′ E	24o56′ N/ 102o29′ E
Habitat size (100 m²)	5-10	5-10	10-20	5-10	10-20	10-20	10-20
Altitude (m)	1 350	1 700	1 136	1 873	1 456	1 352	2 100
Soil water supply	Rich	Rich	Rich	Poor	Very rich	Poor	Very poor
Degree of disturbance	Slight	Slight	Undisturbed	Intense	Undisturbed	Intense	Slight
No. of fruits per plant (Mean ± 1 SE, n = 30)	1.65 ± 0.37	6.53 ± 0.62	0	0.80 ± 0.228	19.10 ± 1.58	0	0.20 ± 0.12
No. of bulbils per plant (Mean ± 1 SE, n = 30)	20. 12 ± 0.88	24. 67 ± 1.88	30.00 ± 1.90	24.27 ± 1.79	28.63 ± 0.77	18.83 ± 1.28	24.43 ± 1.30
Ratio of fruits/bulbils (Mean ± 1 SE, n = 30)	0.11 ± 0.03	0.34 ± 0.06	0	0.04 ± 0.01	0.67 ± 0.05	0	0.01 ± 0.01

	Pop1	Pop2	Pop3	Pop4	Pop5	Pop6	Pop7
Latitude/longitude	21o53′ N/ 100o58′ E	22o53′ N/ 99o48′ E	24o04′ N/ 97o48′ E	24o05′ N/ 99o44′ E	24o25′ N/ 97o50′ E	24o35′ N/ 97o40′ E	24o56′ N/ 102o29′ E
Habitat size (100 m²)	5-10	5-10	10-20	5-10	10-20	10-20	10-20
Altitude (m)	1 350	1 700	1 136	1 873	1 456	1 352	2 100
Soil water supply	Rich	Rich	Rich	Poor	Very rich	Poor	Very poor
Degree of disturbance	Slight	Slight	Undisturbed	Intense	Undisturbed	Intense	Slight
No. of fruits per plant (Mean ± 1 SE, n = 30)	1.65 ± 0.37	6.53 ± 0.62	0	0.80 ± 0.228	19.10 ± 1.58	0	0.20 ± 0.12
No. of bulbils per plant (Mean ± 1 SE, n = 30)	20. 12 ± 0.88	24. 67 ± 1.88	30.00 ± 1.90	24.27 ± 1.79	28.63 ± 0.77	18.83 ± 1.28	24.43 ± 1.30
Ratio of fruits/bulbils (Mean ± 1 SE, n = 30)	0.11 ± 0.03	0.34 ± 0.06	0	0.04 ± 0.01	0.67 ± 0.05	0	0.01 ± 0.01