植物生态学报 ›› 2008, Vol. 32 ›› Issue (4): 938-950.DOI: 10.3773/j.issn.1005-264x.2008.04.024
所属专题： 生物多样性
严茂粉, 李向华, 王克晶(
)
收稿日期:2006-10-18
接受日期:2007-11-06
出版日期:2008-10-18
发布日期:2008-07-30
通讯作者:
王克晶
作者简介:*E-mail:wangkj@caas.net.cn基金资助:
YAN Mao-Fen, LI Xiang-Hua, WANG Ke-Jing()
Received:2006-10-18
Accepted:2007-11-06
Online:2008-10-18
Published:2008-07-30
Contact:
WANG Ke-Jing
摘要：
使用40对SSR引物分析了北京地区野生大豆(Glycine soja)天然种群的遗传结构与遗传多样性。10个种群共检测到526个等位变异, 平均每对引物等位基因数为13.15个, 种群平均Shannon指数(I)为0.658, 群体平均位点预期杂合度(He)为0.369, 群体平均位点杂合度(Ho)为1.29 %。平均种群内遗传多样度(Hs)为0.362, 平均种群间遗传多样度(DST)为0.446, 基因分化程度(GST)为0.544。该研究显示, 中-西部生态区种群比北部和东部山区种群有较高的遗传多样性。在地理上, 环绕北京地区的太行山和燕山两大余脉区域野生大豆种群遗传分化表现出地理差异。可能是经过干旱选择而形成的有抗旱潜力的种群在遗传上表现单一化。期待该种群提供耐旱基因。
严茂粉, 李向华, 王克晶. 北京地区野生大豆种群SSR标记的遗传多样性评价. 植物生态学报, 2008, 32(4): 938-950. DOI: 10.3773/j.issn.1005-264x.2008.04.024
YAN Mao-Fen, LI Xiang-Hua, WANG Ke-Jing. EVALUATION OF GENETIC DIVERSITY BY SSR MARKERS FOR NATURAL POPULATIONS OF WILD SOYBEAN (GLYCINE SOJA) GROWING IN THE REGION OF BEIJING, CHINA. Chinese Journal of Plant Ecology, 2008, 32(4): 938-950. DOI: 10.3773/j.issn.1005-264x.2008.04.024
图1 北京采样点示意图
Fig. 1 Collection sites of wild soybean in Beijing
| 种群Population | 收集地点 Collection site | 取样株数 Size of sample | 纬度Latitude (N) | 海拔Altitude (m) | 生境 Habitat | 分布面积 Area (m2) |
|---|---|---|---|---|---|---|
| 1 | 延庆白河堡村 Baihepu, Yanqing | 30 | 40°37′847′′ | 586 | 白河水库边 Baihe Reservoir | 3 000 |
| 2 | 怀柔牦牛沟 Maoniugou, Huairou | 28 | 40°42′534′′ | 322 | 小河岸边 Riverside | 400 |
| 3 | 密云石城镇 Shicheng, Miyun | 30 | 40°33′741′′ | 135 | 白河岸边 Riverside | 3 000 |
| 4 | 平谷刘家河村 Liujiahe, Pingu | 30 | 40°11′871′′ | 89 | 干河堤 Drought riverbank | 1 000 |
| 5 | 顺义北营村 Beiying, Shunyi | 30 | 40°09′048′′ | 68 | 路边 Roadside | 1 000 |
| 6 | 石景山麻峪村 Mayu, Shijingshan | 30 | 39°56′253′′ | 91 | 永定河 River | 2 000 |
| 7 | 门头沟苇甸村 Weidian, Mentougou | 30 | 39°58′641′′ | 150 | 山区路边 Roadside | 500 |
| 8 | 昌平北小营村 Beixiaoying, Changping | 30 | 40°10′846′′ | 59 | 路旁沟中 Roadside trench | 1 000 |
| 9 | 朝阳三岔河村 Sanchahe, Chaoyang | 30 | 39°58′188′′ | 19 | 坝河岸上 Riverbank | 1 000 |
| 10 | 丰台卢沟桥 Lugouqiao, Fengtai | 30 | 39°50′819′′ | 26 | 永定河河床 River bed | 2 000 |
表1 北京10个天然野生大豆种群收集点和生境
Table 1 Collection sites and habitats of 10 natural populations of wild soybean in Beijing
| 种群Population | 收集地点 Collection site | 取样株数 Size of sample | 纬度Latitude (N) | 海拔Altitude (m) | 生境 Habitat | 分布面积 Area (m2) |
|---|---|---|---|---|---|---|
| 1 | 延庆白河堡村 Baihepu, Yanqing | 30 | 40°37′847′′ | 586 | 白河水库边 Baihe Reservoir | 3 000 |
| 2 | 怀柔牦牛沟 Maoniugou, Huairou | 28 | 40°42′534′′ | 322 | 小河岸边 Riverside | 400 |
| 3 | 密云石城镇 Shicheng, Miyun | 30 | 40°33′741′′ | 135 | 白河岸边 Riverside | 3 000 |
| 4 | 平谷刘家河村 Liujiahe, Pingu | 30 | 40°11′871′′ | 89 | 干河堤 Drought riverbank | 1 000 |
| 5 | 顺义北营村 Beiying, Shunyi | 30 | 40°09′048′′ | 68 | 路边 Roadside | 1 000 |
| 6 | 石景山麻峪村 Mayu, Shijingshan | 30 | 39°56′253′′ | 91 | 永定河 River | 2 000 |
| 7 | 门头沟苇甸村 Weidian, Mentougou | 30 | 39°58′641′′ | 150 | 山区路边 Roadside | 500 |
| 8 | 昌平北小营村 Beixiaoying, Changping | 30 | 40°10′846′′ | 59 | 路旁沟中 Roadside trench | 1 000 |
| 9 | 朝阳三岔河村 Sanchahe, Chaoyang | 30 | 39°58′188′′ | 19 | 坝河岸上 Riverbank | 1 000 |
| 10 | 丰台卢沟桥 Lugouqiao, Fengtai | 30 | 39°50′819′′ | 26 | 永定河河床 River bed | 2 000 |
图2 引物satt236扩增一个种群部分的电泳多态带
Fig. 2 Part electrophoretic profiles of a population amplified by satt236 primers
| 引物 Primer | 带数 No. of bands | 种群 Population | 引物 Primer | 带数 No. of bands | 种群 Population | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||||
| satt236 | 9 | 5 | 1 | 7 | 1 | 7 | 1 | 4 | 5 | 5 | 2 | satt309 | 12 | 2 | 1 | 4 | 2 | 2 | 1 | 3 | 3 | 3 | 1 | |
| satt300 | 16 | 3 | 1 | 4 | 2 | 3 | 2 | 6 | 7 | 2 | 2 | satt279 | 14 | 2 | 1 | 2 | 1 | 5 | 3 | 5 | 2 | 3 | 3 | |
| satt390 | 13 | 1 | 1 | 4 | 1 | 5 | 2 | 4 | 7 | 3 | 3 | satt434 | 11 | 3 | 1 | 3 | 4 | 4 | 1 | 8 | 8 | 2 | 2 | |
| satt429 | 12 | 4 | 1 | 8 | 1 | 7 | 1 | 3 | 3 | 3 | 3 | satt239 | 10 | 2 | 1 | 4 | 2 | 3 | 1 | 2 | 3 | 2 | 2 | |
| satt453 | 11 | 2 | 1 | 7 | 1 | 5 | 2 | 3 | 4 | 2 | 2 | satt571 | 18 | 2 | 1 | 6 | 1 | 8 | 1 | 4 | 3 | 3 | 2 | |
| satt197 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | satt431 | 17 | 3 | 2 | 3 | 1 | 6 | 5 | 4 | 4 | 5 | 3 | |
| att168 | 12 | 1 | 2 | 2 | 1 | 5 | 2 | 5 | 5 | 4 | 5 | satt414 | 17 | 1 | 1 | 4 | 2 | 6 | 2 | 5 | 4 | 1 | 2 | |
| satt556 | 16 | 4 | 1 | 6 | 1 | 8 | 4 | 6 | 4 | 4 | 4 | satt242 | 13 | 3 | 1 | 2 | 1 | 5 | 2 | 3 | 4 | 4 | 2 | |
| satt194 | 14 | 4 | 1 | 5 | 1 | 3 | 3 | 4 | 3 | 2 | 2 | sct-099 | 15 | 2 | 1 | 4 | 1 | 4 | 3 | 5 | 7 | 4 | 4 | |
| Satt180 | 9 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 2 | 1 | 2 | satt373 | 12 | 2 | 1 | 4 | 1 | 4 | 3 | 2 | 4 | 3 | 1 | |
| satt307 | 14 | 6 | 2 | 6 | 1 | 2 | 1 | 3 | 3 | 3 | 3 | satt346 | 16 | 2 | 1 | 4 | 1 | 1 | 2 | 4 | 3 | 3 | 2 | |
| satt281 | 19 | 4 | 1 | 6 | 1 | 6 | 3 | 3 | 6 | 4 | 5 | satt590 | 13 | 2 | 1 | 4 | 1 | 2 | 1 | 4 | 7 | 3 | 3 | |
| satt386 | 15 | 3 | 1 | 2 | 1 | 3 | 3 | 3 | 6 | 3 | 2 | satt339 | 8 | 3 | 1 | 3 | 1 | 3 | 2 | 4 | 3 | 1 | 3 | |
| satt002 | 17 | 2 | 1 | 7 | 1 | 6 | 2 | 7 | 5 | 3 | 1 | satt530 | 19 | 5 | 1 | 6 | 1 | 5 | 1 | 5 | 5 | 4 | 1 | |
| satt268 | 11 | 3 | 2 | 4 | 1 | 3 | 1 | 5 | 5 | 3 | 4 | satt243 | 12 | 4 | 1 | 5 | 1 | 7 | 4 | 3 | 4 | 3 | 5 | |
| satt230 | 9 | 9 | 1 | 6 | 1 | 4 | 4 | 4 | 3 | 4 | 4 | satt487 | 16 | 3 | 1 | 6 | 1 | 6 | 3 | 6 | 5 | 3 | 3 | |
| satt185 | 13 | 4 | 1 | 4 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | satt345 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | |
| satt146 | 14 | 2 | 1 | 6 | 2 | 3 | 1 | 4 | 1 | 3 | 2 | satt216 | 15 | 5 | 1 | 2 | 1 | 1 | 2 | 5 | 4 | 4 | 3 | |
| satt334 | 10 | 2 | 1 | 2 | 1 | 3 | 1 | 3 | 1 | 1 | 1 | satt005 | 14 | 2 | 2 | 8 | 1 | 3 | 3 | 5 | 3 | 3 | 2 | |
| satt352 | 11 | 3 | 1 | 4 | 1 | 3 | 1 | 4 | 2 | 2 | 2 | satt267 | 8 | 1 | 1 | 4 | 1 | 5 | 2 | 2 | 3 | 2 | 3 | |
| 各居群在40对引物上的总带数 Total | 526 | 116 | 45 | 175 | 48 | 165 | 80 | 157 | 157 | 113 | 100 | |||||||||||||
表2 各个引物在每个种群中产生的带数
Table 2 The number of bands produced by every primer in the populations
| 引物 Primer | 带数 No. of bands | 种群 Population | 引物 Primer | 带数 No. of bands | 种群 Population | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |||||
| satt236 | 9 | 5 | 1 | 7 | 1 | 7 | 1 | 4 | 5 | 5 | 2 | satt309 | 12 | 2 | 1 | 4 | 2 | 2 | 1 | 3 | 3 | 3 | 1 | |
| satt300 | 16 | 3 | 1 | 4 | 2 | 3 | 2 | 6 | 7 | 2 | 2 | satt279 | 14 | 2 | 1 | 2 | 1 | 5 | 3 | 5 | 2 | 3 | 3 | |
| satt390 | 13 | 1 | 1 | 4 | 1 | 5 | 2 | 4 | 7 | 3 | 3 | satt434 | 11 | 3 | 1 | 3 | 4 | 4 | 1 | 8 | 8 | 2 | 2 | |
| satt429 | 12 | 4 | 1 | 8 | 1 | 7 | 1 | 3 | 3 | 3 | 3 | satt239 | 10 | 2 | 1 | 4 | 2 | 3 | 1 | 2 | 3 | 2 | 2 | |
| satt453 | 11 | 2 | 1 | 7 | 1 | 5 | 2 | 3 | 4 | 2 | 2 | satt571 | 18 | 2 | 1 | 6 | 1 | 8 | 1 | 4 | 3 | 3 | 2 | |
| satt197 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | satt431 | 17 | 3 | 2 | 3 | 1 | 6 | 5 | 4 | 4 | 5 | 3 | |
| att168 | 12 | 1 | 2 | 2 | 1 | 5 | 2 | 5 | 5 | 4 | 5 | satt414 | 17 | 1 | 1 | 4 | 2 | 6 | 2 | 5 | 4 | 1 | 2 | |
| satt556 | 16 | 4 | 1 | 6 | 1 | 8 | 4 | 6 | 4 | 4 | 4 | satt242 | 13 | 3 | 1 | 2 | 1 | 5 | 2 | 3 | 4 | 4 | 2 | |
| satt194 | 14 | 4 | 1 | 5 | 1 | 3 | 3 | 4 | 3 | 2 | 2 | sct-099 | 15 | 2 | 1 | 4 | 1 | 4 | 3 | 5 | 7 | 4 | 4 | |
| Satt180 | 9 | 2 | 1 | 3 | 1 | 3 | 1 | 2 | 2 | 1 | 2 | satt373 | 12 | 2 | 1 | 4 | 1 | 4 | 3 | 2 | 4 | 3 | 1 | |
| satt307 | 14 | 6 | 2 | 6 | 1 | 2 | 1 | 3 | 3 | 3 | 3 | satt346 | 16 | 2 | 1 | 4 | 1 | 1 | 2 | 4 | 3 | 3 | 2 | |
| satt281 | 19 | 4 | 1 | 6 | 1 | 6 | 3 | 3 | 6 | 4 | 5 | satt590 | 13 | 2 | 1 | 4 | 1 | 2 | 1 | 4 | 7 | 3 | 3 | |
| satt386 | 15 | 3 | 1 | 2 | 1 | 3 | 3 | 3 | 6 | 3 | 2 | satt339 | 8 | 3 | 1 | 3 | 1 | 3 | 2 | 4 | 3 | 1 | 3 | |
| satt002 | 17 | 2 | 1 | 7 | 1 | 6 | 2 | 7 | 5 | 3 | 1 | satt530 | 19 | 5 | 1 | 6 | 1 | 5 | 1 | 5 | 5 | 4 | 1 | |
| satt268 | 11 | 3 | 2 | 4 | 1 | 3 | 1 | 5 | 5 | 3 | 4 | satt243 | 12 | 4 | 1 | 5 | 1 | 7 | 4 | 3 | 4 | 3 | 5 | |
| satt230 | 9 | 9 | 1 | 6 | 1 | 4 | 4 | 4 | 3 | 4 | 4 | satt487 | 16 | 3 | 1 | 6 | 1 | 6 | 3 | 6 | 5 | 3 | 3 | |
| satt185 | 13 | 4 | 1 | 4 | 1 | 4 | 1 | 3 | 3 | 2 | 2 | satt345 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | |
| satt146 | 14 | 2 | 1 | 6 | 2 | 3 | 1 | 4 | 1 | 3 | 2 | satt216 | 15 | 5 | 1 | 2 | 1 | 1 | 2 | 5 | 4 | 4 | 3 | |
| satt334 | 10 | 2 | 1 | 2 | 1 | 3 | 1 | 3 | 1 | 1 | 1 | satt005 | 14 | 2 | 2 | 8 | 1 | 3 | 3 | 5 | 3 | 3 | 2 | |
| satt352 | 11 | 3 | 1 | 4 | 1 | 3 | 1 | 4 | 2 | 2 | 2 | satt267 | 8 | 1 | 1 | 4 | 1 | 5 | 2 | 2 | 3 | 2 | 3 | |
| 各居群在40对引物上的总带数 Total | 526 | 116 | 45 | 175 | 48 | 165 | 80 | 157 | 157 | 113 | 100 | |||||||||||||
| 种群 Population | 多态位点百分数 P (%) | 平均预期杂合度He | 实际观察杂合度 Ho | 平均等位基因 A | 有效等位基因 Ae | Shanno指数 I |
|---|---|---|---|---|---|---|
| 1 | 87.50 | 0.446 3 | 0.006 7 | 2.900 0 | 2.145 8 | 0.743 9 |
| 2 | 12.50 | 0.017 3 | 0.000 9 | 1.125 0 | 1.023 4 | 0.030 4 |
| 3 | 100.00 | 0.603 1 | 0.018 3 | 4.375 0 | 3.120 8 | 1.137 9 |
| 4 | 15.00 | 0.033 6 | 0.000 0 | 1.200 0 | 1.074 5 | 0.058 8 |
| 5 | 90.00 | 0.580 4 | 0.029 2 | 4.125 0 | 3.011 6 | 1.083 8 |
| 6 | 57.50 | 0.221 7 | 0.004 2 | 2.000 0 | 1.454 8 | 0.365 6 |
| 7 | 95.00 | 0.458 4 | 0.019 2 | 3.925 0 | 2.050 7 | 0.853 4 |
| 8 | 92.50 | 0.575 7 | 0.010 8 | 3.925 0 | 2.799 5 | 1.049 0 |
| 9 | 87.50 | 0.400 7 | 0.025 0 | 2.825 0 | 1.843 6 | 0.677 3 |
| 10 | 82.50 | 0.347 9 | 0.015 0 | 2.500 0 | 1.766 7 | 0.582 4 |
| 平均 Mean | 72.00 | 0.368 5 | 0.012 9 | 2.890 0 | 2.029 1 | 0.658 3 |
表3 10个供试种群的遗传多样性参数
Table 3 Estimates of genetic parameters for 10 natural populations of wild soybean in Beijing
| 种群 Population | 多态位点百分数 P (%) | 平均预期杂合度He | 实际观察杂合度 Ho | 平均等位基因 A | 有效等位基因 Ae | Shanno指数 I |
|---|---|---|---|---|---|---|
| 1 | 87.50 | 0.446 3 | 0.006 7 | 2.900 0 | 2.145 8 | 0.743 9 |
| 2 | 12.50 | 0.017 3 | 0.000 9 | 1.125 0 | 1.023 4 | 0.030 4 |
| 3 | 100.00 | 0.603 1 | 0.018 3 | 4.375 0 | 3.120 8 | 1.137 9 |
| 4 | 15.00 | 0.033 6 | 0.000 0 | 1.200 0 | 1.074 5 | 0.058 8 |
| 5 | 90.00 | 0.580 4 | 0.029 2 | 4.125 0 | 3.011 6 | 1.083 8 |
| 6 | 57.50 | 0.221 7 | 0.004 2 | 2.000 0 | 1.454 8 | 0.365 6 |
| 7 | 95.00 | 0.458 4 | 0.019 2 | 3.925 0 | 2.050 7 | 0.853 4 |
| 8 | 92.50 | 0.575 7 | 0.010 8 | 3.925 0 | 2.799 5 | 1.049 0 |
| 9 | 87.50 | 0.400 7 | 0.025 0 | 2.825 0 | 1.843 6 | 0.677 3 |
| 10 | 82.50 | 0.347 9 | 0.015 0 | 2.500 0 | 1.766 7 | 0.582 4 |
| 平均 Mean | 72.00 | 0.368 5 | 0.012 9 | 2.890 0 | 2.029 1 | 0.658 3 |
| 位点Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传 多样度Hs | 居群间遗传多样度DST | 基因分 化度GST | 位点 Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传多样度Hs | 居群间遗传 多样度DST | 基因分 化度GST |
|---|---|---|---|---|---|---|---|---|---|---|---|
| satt236 | A1 | 0.850 1 | 0.422 4 | 0.345 3 | 0.449 8 | satt309 | G | 0.466 7 | 0.282 3 | 0.569 7 | 0.668 7 |
| satt300 | A1 | 0.746 2 | 0.414 5 | 0.434 8 | 0.512 0 | satt279 | H | 0.756 0 | 0.437 8 | 0.407 5 | 0.482 1 |
| satt390 | A2 | 0.749 5 | 0.402 8 | 0.432 6 | 0.517 8 | satt434 | H | 0.759 6 | 0.403 3 | 0.432 5 | 0.517 5 |
| satt429 | A2 | 0.767 3 | 0.390 7 | 0.443 5 | 0.531 6 | satt239 | I | 0.503 0 | 0.300 2 | 0.424 6 | 0.585 8 |
| satt453 | B1 | 0.710 5 | 0.403 5 | 0.464 6 | 0.535 2 | satt571 | I | 0.693 1 | 0.373 4 | 0.499 4 | 0.572 2 |
| satt197 | B1 | 0.066 5 | 0.042 4 | 0.012 8 | 0.231 4 | satt431 | J | 0.930 3 | 0.502 0 | 0.394 9 | 0.440 3 |
| satt168 | B2 | 0.725 1 | 0.379 8 | 0.479 8 | 0.558 2 | satt414 | K | 0.584 7 | 0.326 5 | 0.580 0 | 0.639 8 |
| satt556 | B2 | 0.927 3 | 0.478 0 | 0.426 1 | 0.471 3 | satt242 | K | 0.682 3 | 0.400 3 | 0.428 1 | 0.516 8 |
| satt194 | C1 | 0.747 1 | 0.442 2 | 0.427 3 | 0.491 4 | sct-099 | L | 0.871 6 | 0.473 8 | 0.413 9 | 0.466 3 |
| Satt180 | C1 | 0.342 8 | 0.220 4 | 0.540 7 | 0.710 4 | satt373 | L | 0.601 4 | 0.346 7 | 0.486 9 | 0.584 1 |
| satt307 | C2 | 0.634 9 | 0.339 7 | 0.484 0 | 0.587 6 | satt346 | M | 0.479 8 | 0.291 4 | 0.587 5 | 0.668 4 |
| satt281 | C2 | 0.932 5 | 0.470 6 | 0.441 8 | 0.484 2 | satt590 | M | 0.651 4 | 0.363 6 | 0.487 7 | 0.572 9 |
| satt386 | D2 | 0.677 0 | 0.394 4 | 0.498 3 | 0.558 2 | satt339 | N | 0.521 9 | 0.305 6 | 0.518 0 | 0.629 0 |
| satt002 | D2 | 0.869 4 | 0.457 4 | 0.455 6 | 0.499 0 | satt530 | N | 0.800 0 | 0.401 8 | 0.473 1 | 0.540 7 |
| satt268 | E | 0.774 0 | 0.427 8 | 0.439 6 | 0.506 8 | satt243 | O | 0.881 3 | 0.464 3 | 0.407 1 | 0.467 2 |
| satt230 | E | 0.967 9 | 0.497 0 | 0.361 8 | 0.421 3 | satt487 | O | 0.795 0 | 0.422 0 | 0.459 0 | 0.521 0 |
| satt185 | E | 0.682 4 | 0.394 9 | 0.489 3 | 0.553 4 | satt345 | O | 0.127 4 | 0.080 0 | 0.078 3 | 0.494 7 |
| satt146 | F | 0.496 3 | 0.282 7 | 0.597 2 | 0.678 7 | satt216 | D1b+W | 0.649 9 | 0.359 5 | 0.525 3 | 0.593 7 |
| satt334 | F | 0.184 7 | 0.107 8 | 0.719 3 | 0.869 7 | satt005 | D1b+W | 0.726 1 | 0.405 1 | 0.452 8 | 0.527 8 |
| satt352 | G | 0.444 3 | 0.246 4 | 0.326 4 | 0.569 8 | satt267 | D1a+Q | 0.552 2 | 0.328 4 | 0.389 5 | 0.542 5 |
| 40个位点各参数的平均 Average genetic parameters for 40 loci | 0.658 2 | 0.362 1 | 0.445 9 | 0.544 2 | |||||||
表4 北京地区野生大豆种群SSR位点的遗传化分
Table 4 Estimates of genetic diversity for SSR loci in natural populations of wild soybean in Beijing
| 位点Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传 多样度Hs | 居群间遗传多样度DST | 基因分 化度GST | 位点 Locus | 连锁群Linkage | Shannon 指数I | 居群内遗传多样度Hs | 居群间遗传 多样度DST | 基因分 化度GST |
|---|---|---|---|---|---|---|---|---|---|---|---|
| satt236 | A1 | 0.850 1 | 0.422 4 | 0.345 3 | 0.449 8 | satt309 | G | 0.466 7 | 0.282 3 | 0.569 7 | 0.668 7 |
| satt300 | A1 | 0.746 2 | 0.414 5 | 0.434 8 | 0.512 0 | satt279 | H | 0.756 0 | 0.437 8 | 0.407 5 | 0.482 1 |
| satt390 | A2 | 0.749 5 | 0.402 8 | 0.432 6 | 0.517 8 | satt434 | H | 0.759 6 | 0.403 3 | 0.432 5 | 0.517 5 |
| satt429 | A2 | 0.767 3 | 0.390 7 | 0.443 5 | 0.531 6 | satt239 | I | 0.503 0 | 0.300 2 | 0.424 6 | 0.585 8 |
| satt453 | B1 | 0.710 5 | 0.403 5 | 0.464 6 | 0.535 2 | satt571 | I | 0.693 1 | 0.373 4 | 0.499 4 | 0.572 2 |
| satt197 | B1 | 0.066 5 | 0.042 4 | 0.012 8 | 0.231 4 | satt431 | J | 0.930 3 | 0.502 0 | 0.394 9 | 0.440 3 |
| satt168 | B2 | 0.725 1 | 0.379 8 | 0.479 8 | 0.558 2 | satt414 | K | 0.584 7 | 0.326 5 | 0.580 0 | 0.639 8 |
| satt556 | B2 | 0.927 3 | 0.478 0 | 0.426 1 | 0.471 3 | satt242 | K | 0.682 3 | 0.400 3 | 0.428 1 | 0.516 8 |
| satt194 | C1 | 0.747 1 | 0.442 2 | 0.427 3 | 0.491 4 | sct-099 | L | 0.871 6 | 0.473 8 | 0.413 9 | 0.466 3 |
| Satt180 | C1 | 0.342 8 | 0.220 4 | 0.540 7 | 0.710 4 | satt373 | L | 0.601 4 | 0.346 7 | 0.486 9 | 0.584 1 |
| satt307 | C2 | 0.634 9 | 0.339 7 | 0.484 0 | 0.587 6 | satt346 | M | 0.479 8 | 0.291 4 | 0.587 5 | 0.668 4 |
| satt281 | C2 | 0.932 5 | 0.470 6 | 0.441 8 | 0.484 2 | satt590 | M | 0.651 4 | 0.363 6 | 0.487 7 | 0.572 9 |
| satt386 | D2 | 0.677 0 | 0.394 4 | 0.498 3 | 0.558 2 | satt339 | N | 0.521 9 | 0.305 6 | 0.518 0 | 0.629 0 |
| satt002 | D2 | 0.869 4 | 0.457 4 | 0.455 6 | 0.499 0 | satt530 | N | 0.800 0 | 0.401 8 | 0.473 1 | 0.540 7 |
| satt268 | E | 0.774 0 | 0.427 8 | 0.439 6 | 0.506 8 | satt243 | O | 0.881 3 | 0.464 3 | 0.407 1 | 0.467 2 |
| satt230 | E | 0.967 9 | 0.497 0 | 0.361 8 | 0.421 3 | satt487 | O | 0.795 0 | 0.422 0 | 0.459 0 | 0.521 0 |
| satt185 | E | 0.682 4 | 0.394 9 | 0.489 3 | 0.553 4 | satt345 | O | 0.127 4 | 0.080 0 | 0.078 3 | 0.494 7 |
| satt146 | F | 0.496 3 | 0.282 7 | 0.597 2 | 0.678 7 | satt216 | D1b+W | 0.649 9 | 0.359 5 | 0.525 3 | 0.593 7 |
| satt334 | F | 0.184 7 | 0.107 8 | 0.719 3 | 0.869 7 | satt005 | D1b+W | 0.726 1 | 0.405 1 | 0.452 8 | 0.527 8 |
| satt352 | G | 0.444 3 | 0.246 4 | 0.326 4 | 0.569 8 | satt267 | D1a+Q | 0.552 2 | 0.328 4 | 0.389 5 | 0.542 5 |
| 40个位点各参数的平均 Average genetic parameters for 40 loci | 0.658 2 | 0.362 1 | 0.445 9 | 0.544 2 | |||||||
图3 北京地区10个野生大豆天然种群的聚类分析
Fig. 3 Dendrogram of 10 natural populations of wild soybean in Beijing based on genetic identity
| 种群Population | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | **** | 0.210 5 | 0.392 6 | 0.193 2 | 0.222 6 | 0.214 9 | 0.218 7 | 0.180 8 | 0.194 3 | 0.192 6 |
| 2 | 1.558 4 | **** | 0.245 5 | 0.053 1 | 0.340 0 | 0.200 3 | 0.210 1 | 0.289 4 | 0.206 8 | 0.194 1 |
| 3 | 0.935 0 | 1.404 6 | **** | 0.266 0 | 0.363 4 | 0.214 9 | 0.211 2 | 0.212 4 | 0.296 1 | 0.276 8 |
| 4 | 1.643 9 | 2.935 0 | 1.324 2 | **** | 0.206 9 | 0.107 0 | 0.170 2 | 0.090 1 | 0.120 8 | 0.152 6 |
| 5 | 1.502 5 | 1.078 8 | 1.012 2 | 1.575 7 | **** | 0.356 6 | 0.265 2 | 0.331 9 | 0.238 2 | 0.241 4 |
| 6 | 1.537 6 | 1.607 8 | 1.537 4 | 2.235 0 | 1.031 0 | **** | 0.253 3 | 0.238 1 | 0.259 8 | 0.195 6 |
| 7 | 1.519 9 | 1.560 3 | 1.554 8 | 1.770 8 | 1.327 2 | 1.373 1 | **** | 0.296 2 | 0.244 6 | 0.176 7 |
| 8 | 1.710 4 | 1.240 1 | 1.549 1 | 2.406 4 | 1.103 1 | 1.434 9 | 1.216 6 | **** | 0.374 8 | 0.340 1 |
| 9 | 1.638 4 | 1.575 9 | 1.217 1 | 2.113 2 | 1.434 7 | 1.348 0 | 1.408 1 | 0.981 5 | **** | 0.457 5 |
| 10 | 1.646 9 | 1.639 4 | 1.284 5 | 1.879 8 | 1.421 1 | 1.631 7 | 1.733 3 | 1.078 5 | 0.781 9 | **** |
表5 10个种群的Nei遗传一致度与遗传距离
Table 5 Nei’s genetic identity and genetic distance among 10 natural populations of wild soybean in Beijing
| 种群Population | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | **** | 0.210 5 | 0.392 6 | 0.193 2 | 0.222 6 | 0.214 9 | 0.218 7 | 0.180 8 | 0.194 3 | 0.192 6 |
| 2 | 1.558 4 | **** | 0.245 5 | 0.053 1 | 0.340 0 | 0.200 3 | 0.210 1 | 0.289 4 | 0.206 8 | 0.194 1 |
| 3 | 0.935 0 | 1.404 6 | **** | 0.266 0 | 0.363 4 | 0.214 9 | 0.211 2 | 0.212 4 | 0.296 1 | 0.276 8 |
| 4 | 1.643 9 | 2.935 0 | 1.324 2 | **** | 0.206 9 | 0.107 0 | 0.170 2 | 0.090 1 | 0.120 8 | 0.152 6 |
| 5 | 1.502 5 | 1.078 8 | 1.012 2 | 1.575 7 | **** | 0.356 6 | 0.265 2 | 0.331 9 | 0.238 2 | 0.241 4 |
| 6 | 1.537 6 | 1.607 8 | 1.537 4 | 2.235 0 | 1.031 0 | **** | 0.253 3 | 0.238 1 | 0.259 8 | 0.195 6 |
| 7 | 1.519 9 | 1.560 3 | 1.554 8 | 1.770 8 | 1.327 2 | 1.373 1 | **** | 0.296 2 | 0.244 6 | 0.176 7 |
| 8 | 1.710 4 | 1.240 1 | 1.549 1 | 2.406 4 | 1.103 1 | 1.434 9 | 1.216 6 | **** | 0.374 8 | 0.340 1 |
| 9 | 1.638 4 | 1.575 9 | 1.217 1 | 2.113 2 | 1.434 7 | 1.348 0 | 1.408 1 | 0.981 5 | **** | 0.457 5 |
| 10 | 1.646 9 | 1.639 4 | 1.284 5 | 1.879 8 | 1.421 1 | 1.631 7 | 1.733 3 | 1.078 5 | 0.781 9 | **** |
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