植物生态学报 ›› 2011, Vol. 35 ›› Issue (3): 330-336.DOI: 10.3724/SP.J.1258.2011.00330
所属专题: 生物多样性
收稿日期:
2010-06-04
接受日期:
2010-11-22
出版日期:
2011-06-04
发布日期:
2011-03-02
通讯作者:
胡庭兴
作者简介:
*E-mail: hutx001@yahoo.com.cn
ZHANG Wei1,2, LUO Jian-Xun2, GU Yun-Jie2, HU Ting-Xing1,*()
Received:
2010-06-04
Accepted:
2010-11-22
Online:
2011-06-04
Published:
2011-03-02
Contact:
HU Ting-Xing
摘要:
为了揭示麻疯树(Jatropha curcas)天然种群的遗传多样性和遗传结构, 采用聚苯烯酰胺凝胶垂直平板电泳技术, 对采自四川、云南、贵州3个省的10个麻疯树天然种群的叶片样本进行了同工酶分析。7个酶系统10个位点的检测结果表明: 麻疯树种群水平上的遗传多样性较高, 每位点平均等位基因数为2.428 6, 多态位点百分率为97.14%, 平均期望杂合度为0.396 4。种群间遗传分化系数为0.041 3, 种群间总的基因流较高, 为5.808 9, 种群间遗传一致度较高(Shannon信息指数为0.921 7- 0.995 3)。非加权类平均法(UPGMA)聚类结果显示, 10个种群的遗传距离与地理距离相关性不显著。麻疯树天然种群具有较低程度的遗传分化、较高的基因流, 种内及种群内多样性丰富, 这为麻疯树优良品种的选育提供了良好的遗传基础。
张炜, 罗建勋, 辜云杰, 胡庭兴. 西南地区麻疯树天然种群遗传多样性的等位酶变异. 植物生态学报, 2011, 35(3): 330-336. DOI: 10.3724/SP.J.1258.2011.00330
ZHANG Wei, LUO Jian-Xun, GU Yun-Jie, HU Ting-Xing. Allozyme variation of genetic diversity in natural populations of Jatropha curcas germplasm from different areas in southwest China. Chinese Journal of Plant Ecology, 2011, 35(3): 330-336. DOI: 10.3724/SP.J.1258.2011.00330
种群代号 Population code | 采样地 Seed collection site | 经纬度 Longitude and latitude | 海拔 Altitude (m) | 水系 River system | 家系数量 No. of families |
---|---|---|---|---|---|
LS | 卢水 Lushui | 98°08′ E, 25°08′ N | 850 | 怒江 Nujiang River | 30 |
SB | 双柏 Shuangbo | 101°51′ E, 25°49′ N | 1 066 | 红河 Honghe River | 30 |
YNPE | 普洱 Puer | 100°59′ E, 22°42′ N | 1 300 | 澜沧江 Lancang River | 30 |
XSBN | 西双版纳 Xishuangbanna | 100°80′ E, 22°35′ N | 1 000 | 澜沧江 Lancang River | 30 |
PJ | 坡脚 Pojiao | 102°55′ E, 26°74′ N | 1 080 | 金沙江 Jinsha River | 30 |
FY | 泸西 Luxi | 103°76′ E, 24°52′N | 1 100 | 南盘江 Nanpan River | 30 |
LB | 雷波 Leibo | 103°62′ E, 28°21′ N | 960 | 金沙江 Jinsha River | 30 |
HPZ | 花棚子 Huapengzi | 101°55′ E, 26°22′ N | 983 | 金沙江 Jinsha River | 30 |
HL | 会理 Huili | 101°55′ E, 26°10′ N | 943 | 金沙江 Jinsha River | 30 |
YL | 金河 Jinhe | 101°51′ E, 27°42′ N | 1 200 | 雅砻江 Yagong River | 30 |
表1 麻疯树采样种群的地理位置和样本量
Table 1 Geographic locations and sample size of Jatropha curcas populations
种群代号 Population code | 采样地 Seed collection site | 经纬度 Longitude and latitude | 海拔 Altitude (m) | 水系 River system | 家系数量 No. of families |
---|---|---|---|---|---|
LS | 卢水 Lushui | 98°08′ E, 25°08′ N | 850 | 怒江 Nujiang River | 30 |
SB | 双柏 Shuangbo | 101°51′ E, 25°49′ N | 1 066 | 红河 Honghe River | 30 |
YNPE | 普洱 Puer | 100°59′ E, 22°42′ N | 1 300 | 澜沧江 Lancang River | 30 |
XSBN | 西双版纳 Xishuangbanna | 100°80′ E, 22°35′ N | 1 000 | 澜沧江 Lancang River | 30 |
PJ | 坡脚 Pojiao | 102°55′ E, 26°74′ N | 1 080 | 金沙江 Jinsha River | 30 |
FY | 泸西 Luxi | 103°76′ E, 24°52′N | 1 100 | 南盘江 Nanpan River | 30 |
LB | 雷波 Leibo | 103°62′ E, 28°21′ N | 960 | 金沙江 Jinsha River | 30 |
HPZ | 花棚子 Huapengzi | 101°55′ E, 26°22′ N | 983 | 金沙江 Jinsha River | 30 |
HL | 会理 Huili | 101°55′ E, 26°10′ N | 943 | 金沙江 Jinsha River | 30 |
YL | 金河 Jinhe | 101°51′ E, 27°42′ N | 1 200 | 雅砻江 Yagong River | 30 |
酶系统 Enzyme system | 缩写 Abbreviation | 标准代号 EC No. | 位点数 No. of loci |
---|---|---|---|
谷草转氨酶 Glutamic-oxalacetic transaminase | GOT | (E.C.2.6.1.1) | 2 |
莽草酸脱氢酶 Shikimate acid dehydrogenase | SHDH | (E.C.1.1.1.25) | 1 |
6-磷酸葡萄糖脱氢酶 Phosphoglucose dehydrogenase | 6PGD | (E.C 1.1.1.44) | 2 |
丙氨酸氨基肽酶 Alanine aminopeptidase | AAP | (E.C.3.4.11. I) | 2 |
磷酸葡萄糖变位酶 Phosphogluco mutase | PGM | (E.C.5.4.2.23) | 3 |
磷酸葡萄糖异构酶 Phosphogluco isomerase | PGI | (E.C.5.3.1.9) | 4 |
甲基萘醌还原酶 Menadione reductase | MNR | (E.C.1.6.99.2) | 7 |
表2 7种酶系统和每个酶系统检测到的位点数
Table 2 Seven enzyme systems assayed and No. of loci detected in each enzyme system
酶系统 Enzyme system | 缩写 Abbreviation | 标准代号 EC No. | 位点数 No. of loci |
---|---|---|---|
谷草转氨酶 Glutamic-oxalacetic transaminase | GOT | (E.C.2.6.1.1) | 2 |
莽草酸脱氢酶 Shikimate acid dehydrogenase | SHDH | (E.C.1.1.1.25) | 1 |
6-磷酸葡萄糖脱氢酶 Phosphoglucose dehydrogenase | 6PGD | (E.C 1.1.1.44) | 2 |
丙氨酸氨基肽酶 Alanine aminopeptidase | AAP | (E.C.3.4.11. I) | 2 |
磷酸葡萄糖变位酶 Phosphogluco mutase | PGM | (E.C.5.4.2.23) | 3 |
磷酸葡萄糖异构酶 Phosphogluco isomerase | PGI | (E.C.5.3.1.9) | 4 |
甲基萘醌还原酶 Menadione reductase | MNR | (E.C.1.6.99.2) | 7 |
位点 Locus | 等位基因 Allele | 种群 Population | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
金河 YL | 坡脚 PJ | 花棚子 HPZ | 雷波 LB | 法依 FY | 双柏 SB | 版纳 XSBN | 普洱 YNPE | 会理 HL | 卢水 LS | 平均 Mean | ||
AAP-1 | A | 0.725 | 0.675 | 0.650 | 0.750 | 0500 | 0.675 | 0.600 | 0.750 | 0.550 | 0.650 | 0.652 |
B | 0.275 | 0.325 | 0.350 | 0.250 | 0.500 | 0.325 | 0.400 | 0.250 | 0.450 | 0.350 | 0.348 | |
SHDH | A | 0.300 | 0.350 | 0.300 | 0.600 | 0.600 | 0.775 | 0.600 | 0.500 | 0.525 | 0.325 | 0.487 |
B | 0.675 | 0.500 | 0.500 | 0.400 | 0.400 | 0.175 | 0.400 | 0.500 | 0.450 | 0.675 | 0.467 | |
C | 0.025 | 0.150 | 0.200 | 0.000 | 0.000 | 0.050 | 0.000 | 0.000 | 0.025 | 0.000 | 0.045 | |
GOT-2 | A | 0.425 | 0.375 | 0.325 | 0.350 | 0.350 | 0.350 | 0.300 | 0.400 | 0.325 | 0.275 | 0.347 |
B | 0.575 | 0.625 | 0.675 | 0.650 | 0.650 | 0.650 | 0.700 | 0.600 | 0.625 | 0.725 | 0.647 | |
PGI-4 | A | 0.625 | 0.500 | 0.450 | 0.550 | 0.475 | 0.525 | 0.575 | 0.500 | 0.550 | 0.550 | 0.530 |
B | 0.375 | 0.500 | 0.500 | 0.375 | 0.475 | 0.350 | 0.425 | 0.500 | 0.450 | 0.450 | 0.440 | |
C | 0.000 | 0.000 | 0.050 | 0.075 | 0.050 | 0.125 | 0.000 | 0.000 | 0.000 | 0.000 | 0.030 | |
6PGD-1 | A | 1.000 | 0.600 | 0.700 | 0.875 | 0.875 | 0.750 | 0.650 | 0.800 | 0.750 | 1.000 | 0.800 |
B | 0.000 | 0.400 | 0.300 | 0.125 | 0.125 | 0.250 | 0.350 | 0.200 | 0.250 | 0.000 | 0.200 | |
MNR-3 | A | 0. 250 | 0.175 | 0.175 | 0.200 | 0.175 | 0.150 | 0.200 | 0.275 | 0.250 | 0.175 | 0.177 |
B | 0.750 | 0.825 | 0.825 | 0.800 | 0.825 | 0.850 | 0.800 | 0.725 | 0.750 | 0.825 | 0.797 | |
PGM-2 | A | 0.700 | 0.875 | 0.850 | 0.700 | 0.825 | 0.650 | 0.700 | 0.625 | 0.575 | 0.675 | 0.718 |
B | 0.300 | 0.125 | 0.150 | 0.300 | 0.175 | 0.325 | 0.300 | 0.375 | 0.425 | 0.325 | 0.280 | |
C | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.025 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 | |
基因频率平均值 Mean value of gene frequency | 0.397 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.409 | 0.412 | 0.410 | |
等位基因数 Number of alleles | 14 | 15 | 16 | 15 | 15 | 17 | 15 | 14 | 15 | 13 | 14.9 |
表3 10个麻疯树种群等位基因的频率分布
Table 3 Allele frequencies in ten natural populations of Jatropha curcas
位点 Locus | 等位基因 Allele | 种群 Population | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
金河 YL | 坡脚 PJ | 花棚子 HPZ | 雷波 LB | 法依 FY | 双柏 SB | 版纳 XSBN | 普洱 YNPE | 会理 HL | 卢水 LS | 平均 Mean | ||
AAP-1 | A | 0.725 | 0.675 | 0.650 | 0.750 | 0500 | 0.675 | 0.600 | 0.750 | 0.550 | 0.650 | 0.652 |
B | 0.275 | 0.325 | 0.350 | 0.250 | 0.500 | 0.325 | 0.400 | 0.250 | 0.450 | 0.350 | 0.348 | |
SHDH | A | 0.300 | 0.350 | 0.300 | 0.600 | 0.600 | 0.775 | 0.600 | 0.500 | 0.525 | 0.325 | 0.487 |
B | 0.675 | 0.500 | 0.500 | 0.400 | 0.400 | 0.175 | 0.400 | 0.500 | 0.450 | 0.675 | 0.467 | |
C | 0.025 | 0.150 | 0.200 | 0.000 | 0.000 | 0.050 | 0.000 | 0.000 | 0.025 | 0.000 | 0.045 | |
GOT-2 | A | 0.425 | 0.375 | 0.325 | 0.350 | 0.350 | 0.350 | 0.300 | 0.400 | 0.325 | 0.275 | 0.347 |
B | 0.575 | 0.625 | 0.675 | 0.650 | 0.650 | 0.650 | 0.700 | 0.600 | 0.625 | 0.725 | 0.647 | |
PGI-4 | A | 0.625 | 0.500 | 0.450 | 0.550 | 0.475 | 0.525 | 0.575 | 0.500 | 0.550 | 0.550 | 0.530 |
B | 0.375 | 0.500 | 0.500 | 0.375 | 0.475 | 0.350 | 0.425 | 0.500 | 0.450 | 0.450 | 0.440 | |
C | 0.000 | 0.000 | 0.050 | 0.075 | 0.050 | 0.125 | 0.000 | 0.000 | 0.000 | 0.000 | 0.030 | |
6PGD-1 | A | 1.000 | 0.600 | 0.700 | 0.875 | 0.875 | 0.750 | 0.650 | 0.800 | 0.750 | 1.000 | 0.800 |
B | 0.000 | 0.400 | 0.300 | 0.125 | 0.125 | 0.250 | 0.350 | 0.200 | 0.250 | 0.000 | 0.200 | |
MNR-3 | A | 0. 250 | 0.175 | 0.175 | 0.200 | 0.175 | 0.150 | 0.200 | 0.275 | 0.250 | 0.175 | 0.177 |
B | 0.750 | 0.825 | 0.825 | 0.800 | 0.825 | 0.850 | 0.800 | 0.725 | 0.750 | 0.825 | 0.797 | |
PGM-2 | A | 0.700 | 0.875 | 0.850 | 0.700 | 0.825 | 0.650 | 0.700 | 0.625 | 0.575 | 0.675 | 0.718 |
B | 0.300 | 0.125 | 0.150 | 0.300 | 0.175 | 0.325 | 0.300 | 0.375 | 0.425 | 0.325 | 0.280 | |
C | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.025 | 0.000 | 0.000 | 0.000 | 0.000 | 0.002 | |
基因频率平均值 Mean value of gene frequency | 0.397 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.412 | 0.409 | 0.412 | 0.410 | |
等位基因数 Number of alleles | 14 | 15 | 16 | 15 | 15 | 17 | 15 | 14 | 15 | 13 | 14.9 |
种群 Population | 个体 Individual | 等位基因数 Number of alleles | 多态位点百分率 P | Shannon信息指数 I | 观测杂合度 Ho | 期望杂合度 He |
---|---|---|---|---|---|---|
LS | 30 | 2.0 (0.2) | 85.7 | 0.546 2 | 0.493 (0.092) | 0.382 (0.065) |
SB | 30 | 2.1 (0.1) | 100.0 | 0.642 5 | 0.600 (0.080) | 0.440 (0.051) |
YNPE | 30 | 2.3 (0.2) | 100.0 | 0.665 8 | 0.600 (0.076) | 0.443 (0.050) |
XSBN | 30 | 2.1 (0.1) | 100.0 | 0.608 8 | 0.564 (0.090) | 0.413 (0.043) |
PJ | 30 | 2.1 (0.1) | 100.0 | 0.596 4 | 0.529 (0.091) | 0.407 (0.050) |
FY | 30 | 2.4 (0.2) | 100.0 | 0.659 8 | 0.550 (0.089) | 0.432 (0.040) |
LB | 30 | 2.0 ( 0.0) | 100.0 | 0.628 2 | 0.679 (0.060) | 0.449 (0.023) |
HPZ | 30 | 2.0 (0.0) | 100.0 | 0.624 5 | 0.629 (0.055) | 0.446 (0.027) |
HL | 30 | 2.1 (0.1) | 100.0 | 0.662 0 | 0.679 (0.068) | 0.472 (0.023) |
YL | 30 | 1.9 ( 0.1) | 85.7 | 0.521 2 | 0.471 (0.091) | 0.368 (0.066) |
平均 | 30 | 2.1 (0.1) | 97.1 | 0.615 5 | 0.579 (0.079) | 0.425 (0.043) |
表4 10个麻疯树天然种群的遗传多样性
Table 4 Genetic diversity in ten natural populations of Jatropha curcas
种群 Population | 个体 Individual | 等位基因数 Number of alleles | 多态位点百分率 P | Shannon信息指数 I | 观测杂合度 Ho | 期望杂合度 He |
---|---|---|---|---|---|---|
LS | 30 | 2.0 (0.2) | 85.7 | 0.546 2 | 0.493 (0.092) | 0.382 (0.065) |
SB | 30 | 2.1 (0.1) | 100.0 | 0.642 5 | 0.600 (0.080) | 0.440 (0.051) |
YNPE | 30 | 2.3 (0.2) | 100.0 | 0.665 8 | 0.600 (0.076) | 0.443 (0.050) |
XSBN | 30 | 2.1 (0.1) | 100.0 | 0.608 8 | 0.564 (0.090) | 0.413 (0.043) |
PJ | 30 | 2.1 (0.1) | 100.0 | 0.596 4 | 0.529 (0.091) | 0.407 (0.050) |
FY | 30 | 2.4 (0.2) | 100.0 | 0.659 8 | 0.550 (0.089) | 0.432 (0.040) |
LB | 30 | 2.0 ( 0.0) | 100.0 | 0.628 2 | 0.679 (0.060) | 0.449 (0.023) |
HPZ | 30 | 2.0 (0.0) | 100.0 | 0.624 5 | 0.629 (0.055) | 0.446 (0.027) |
HL | 30 | 2.1 (0.1) | 100.0 | 0.662 0 | 0.679 (0.068) | 0.472 (0.023) |
YL | 30 | 1.9 ( 0.1) | 85.7 | 0.521 2 | 0.471 (0.091) | 0.368 (0.066) |
平均 | 30 | 2.1 (0.1) | 97.1 | 0.615 5 | 0.579 (0.079) | 0.425 (0.043) |
位点 Locus | 杂合度 Heterozygosity | 种群 Population | 平均值 Mean | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
金河 YL | 坡脚 PJ | 花棚子 HPZ | 雷波 LB | 法依 FY | 双柏 SB | 版纳 XSBN | 普洱 YNPE | 会理 HL | 卢水 LS | |||
AAP-1 | Ho | 0.450 | 0.350 | 0.300 | 0.500 | 0.400 | 0.350 | 0.200 | 0.500 | 0.100 | 0.400 | 0.355 |
He | 0.550 | 0.650 | 0.700 | 0.500 | 0.600 | 0.650 | 0.800 | 0.500 | 0.900 | 0.600 | 0.645 | |
SHDH | Ho | 0.600 | 0.300 | 0.200 | 0.200 | 0.200 | 0.550 | 0.200 | 0.300 | 0.150 | 0.550 | 0.325 |
He | 0.400 | 0.700 | 0.800 | 0.800 | 0.800 | 0.450 | 0.800 | 0.700 | 0.850 | 0.450 | 0.675 | |
GOT-2 | Ho | 0.350 | 0.250 | 0.350 | 0.300 | 0.300 | 0.300 | 0.400 | 0.200 | 0.250 | 0.450 | 0.315 |
He | 0.650 | 0.750 | 0.650 | 0.700 | 0.700 | 0.700 | 0.600 | 0.800 | 0.750 | 0.550 | 0.685 | |
PGI-4 | Ho | 0.250 | 0.300 | 0.200 | 0.100 | 0.250 | 0.500 | 0.150 | 0.300 | 0.300 | 0.300 | 0.265 |
He | 0.750 | 0.700 | 0.800 | 0.900 | 0.050 | 0.950 | 0.850 | 0.700 | 0.700 | 0.700 | 0.710 | |
6PGD-1 | Ho | 1.000 | 0.200 | 0.400 | 0.750 | 0.750 | 0.500 | 0.300 | 0.600 | 0.500 | 1.000 | 0.600 |
He | 0.000 | 0.800 | 0.600 | 0.250 | 0.250 | 0.500 | 0.700 | 0.400 | 0.500 | 0.000 | 0.400 | |
MNR-3 | Ho | 0.500 | 0.650 | 0.650 | 0.600 | 0.650 | 0.700 | 0.600 | 0.450 | 0.600 | 0.650 | 0.605 |
He | 0.500 | 0.350 | 0.350 | 0.400 | 0.350 | 0.300 | 0.400 | 0.550 | 0.400 | 0.350 | 0.395 | |
PGM-2 | Ho | 0.400 | 0.750 | 0.700 | 0.600 | 0.750 | 0.700 | 0.400 | 0.250 | 0.350 | 0.350 | 0.525 |
He | 0.600 | 0.250 | 0.300 | 0.400 | 0.250 | 0.300 | 0.600 | 0.750 | 0.650 | 0.650 | 0.475 | |
平均值 Mean | Ho | 0.507 | 0.400 | 0.400 | 0.436 | 0.471 | 0.514 | 0.321 | 0.371 | 0.321 | 0.528 | 0.427 |
He | 0.493 | 0.600 | 0.600 | 0.564 | 0.429 | 0.550 | 0.679 | 0.628 | 0.678 | 0.471 | 0.569 |
表5 麻疯树10个种群的单位点杂合度
Table 5 Observed heterozygosity at single locus detected in ten populations of Jatropha curcas
位点 Locus | 杂合度 Heterozygosity | 种群 Population | 平均值 Mean | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
金河 YL | 坡脚 PJ | 花棚子 HPZ | 雷波 LB | 法依 FY | 双柏 SB | 版纳 XSBN | 普洱 YNPE | 会理 HL | 卢水 LS | |||
AAP-1 | Ho | 0.450 | 0.350 | 0.300 | 0.500 | 0.400 | 0.350 | 0.200 | 0.500 | 0.100 | 0.400 | 0.355 |
He | 0.550 | 0.650 | 0.700 | 0.500 | 0.600 | 0.650 | 0.800 | 0.500 | 0.900 | 0.600 | 0.645 | |
SHDH | Ho | 0.600 | 0.300 | 0.200 | 0.200 | 0.200 | 0.550 | 0.200 | 0.300 | 0.150 | 0.550 | 0.325 |
He | 0.400 | 0.700 | 0.800 | 0.800 | 0.800 | 0.450 | 0.800 | 0.700 | 0.850 | 0.450 | 0.675 | |
GOT-2 | Ho | 0.350 | 0.250 | 0.350 | 0.300 | 0.300 | 0.300 | 0.400 | 0.200 | 0.250 | 0.450 | 0.315 |
He | 0.650 | 0.750 | 0.650 | 0.700 | 0.700 | 0.700 | 0.600 | 0.800 | 0.750 | 0.550 | 0.685 | |
PGI-4 | Ho | 0.250 | 0.300 | 0.200 | 0.100 | 0.250 | 0.500 | 0.150 | 0.300 | 0.300 | 0.300 | 0.265 |
He | 0.750 | 0.700 | 0.800 | 0.900 | 0.050 | 0.950 | 0.850 | 0.700 | 0.700 | 0.700 | 0.710 | |
6PGD-1 | Ho | 1.000 | 0.200 | 0.400 | 0.750 | 0.750 | 0.500 | 0.300 | 0.600 | 0.500 | 1.000 | 0.600 |
He | 0.000 | 0.800 | 0.600 | 0.250 | 0.250 | 0.500 | 0.700 | 0.400 | 0.500 | 0.000 | 0.400 | |
MNR-3 | Ho | 0.500 | 0.650 | 0.650 | 0.600 | 0.650 | 0.700 | 0.600 | 0.450 | 0.600 | 0.650 | 0.605 |
He | 0.500 | 0.350 | 0.350 | 0.400 | 0.350 | 0.300 | 0.400 | 0.550 | 0.400 | 0.350 | 0.395 | |
PGM-2 | Ho | 0.400 | 0.750 | 0.700 | 0.600 | 0.750 | 0.700 | 0.400 | 0.250 | 0.350 | 0.350 | 0.525 |
He | 0.600 | 0.250 | 0.300 | 0.400 | 0.250 | 0.300 | 0.600 | 0.750 | 0.650 | 0.650 | 0.475 | |
平均值 Mean | Ho | 0.507 | 0.400 | 0.400 | 0.436 | 0.471 | 0.514 | 0.321 | 0.371 | 0.321 | 0.528 | 0.427 |
He | 0.493 | 0.600 | 0.600 | 0.564 | 0.429 | 0.550 | 0.679 | 0.628 | 0.678 | 0.471 | 0.569 |
位点 Locus | Fis | Fit | Fst | Nm |
---|---|---|---|---|
AAP-1 | -0.462 2 | -0.422 3 | 0.027 3 | 8.920 6 |
SHDH | -0.365 0 | -0.245 9 | 0.087 2 | 2.615 7 |
GOT-2 | -0.512 6 | -0.500 6 | 0.007 9 | 31.340 8 |
PGI-4 | -0.506 9 | -0.486 8 | 0.013 3 | 18.552 9 |
6PGD-1 | -0.397 4 | -0.250 0 | 0.105 5 | 2.120 4 |
MNR-3 | -0.234 9 | -0.223 0 | 0.009 6 | 25.692 8 |
PGM-2 | -0.221 1 | -0.167 7 | 0.043 7 | 5.467 3 |
Mean | -0.397 7 | -0.340 0 | 0.041 3 | 5.808 9 |
表6 麻疯树天然种群F统计量及基因流
Table 6 Estimated F-statistics and gene flow in natural population of Jatropha curcas
位点 Locus | Fis | Fit | Fst | Nm |
---|---|---|---|---|
AAP-1 | -0.462 2 | -0.422 3 | 0.027 3 | 8.920 6 |
SHDH | -0.365 0 | -0.245 9 | 0.087 2 | 2.615 7 |
GOT-2 | -0.512 6 | -0.500 6 | 0.007 9 | 31.340 8 |
PGI-4 | -0.506 9 | -0.486 8 | 0.013 3 | 18.552 9 |
6PGD-1 | -0.397 4 | -0.250 0 | 0.105 5 | 2.120 4 |
MNR-3 | -0.234 9 | -0.223 0 | 0.009 6 | 25.692 8 |
PGM-2 | -0.221 1 | -0.167 7 | 0.043 7 | 5.467 3 |
Mean | -0.397 7 | -0.340 0 | 0.041 3 | 5.808 9 |
种群代号 Population code | YL | PJ | HPZ | LB | FY | SB | XSBN | YNPE | HL | LS |
---|---|---|---|---|---|---|---|---|---|---|
YL | **** | 0.943 6 | 0.956 1 | 0.973 9 | 0.955 2 | 0.921 7 | 0.943 4 | 0.977 7 | 0.961 7 | 0.990 9 |
PJ | 0.058 1 | **** | 0.995 3 | 0.958 1 | 0.961 7 | 0.941 2 | 0.975 3 | 0.964 5 | 0.959 1 | 0.945 0 |
HPZ | 0.044 9 | 0.004 7 | **** | 0.964 1 | 0.969 9 | 0.941 8 | 0.972 1 | 0.967 6 | 0.962 8 | 0.962 4 |
LB | 0.026 4 | 0.042 8 | 0.036 5 | **** | 0.979 3 | 0.983 3 | 0.980 7 | 0.990 2 | 0.980 0 | 0.974 3 |
FY | 0.045 8 | 0.039 0 | 0.030 5 | 0.020 9 | **** | 0.969 4 | 0.979 3 | 0.968 1 | 0.977 6 | 0.966 8 |
SB | 0.081 5 | 0.060 6 | 0.059 9 | 0.016 9 | 0.031 1 | **** | 0.981 0 | 0.965 3 | 0.970 7 | 0.928 4 |
XSBN | 0.058 3 | 0.025 0 | 0.028 3 | 0.019 5 | 0.020 9 | 0.019 2 | **** | 0.979 3 | 0.989 5 | 0.953 8 |
YNPE | 0.022 6 | 0.036 1 | 0.033 0 | 0.009 8 | 0.032 4 | 0.035 3 | 0.021 0 | **** | 0.987 2 | 0.975 6 |
HL | 0.039 0 | 0.041 8 | 0.037 9 | 0.020 2 | 0.022 7 | 0.029 7 | 0.010 5 | 0.012 9 | **** | 0.968 7 |
LS | 0.009 1 | 0.056 6 | 0.038 3 | 0.026 0 | 0.033 7 | 0.074 3 | 0.047 3 | 0.024 8 | 0.031 8 | **** |
表7 麻疯树种群间的遗传距离D (对角线下方)及遗传一致度I (对角线上方) (Nei, 1978)
Table 7 Genetic distances (below diagonal) and genetic identities (above diagonal) among populations of Jatropha curcas (Nei, 1978)
种群代号 Population code | YL | PJ | HPZ | LB | FY | SB | XSBN | YNPE | HL | LS |
---|---|---|---|---|---|---|---|---|---|---|
YL | **** | 0.943 6 | 0.956 1 | 0.973 9 | 0.955 2 | 0.921 7 | 0.943 4 | 0.977 7 | 0.961 7 | 0.990 9 |
PJ | 0.058 1 | **** | 0.995 3 | 0.958 1 | 0.961 7 | 0.941 2 | 0.975 3 | 0.964 5 | 0.959 1 | 0.945 0 |
HPZ | 0.044 9 | 0.004 7 | **** | 0.964 1 | 0.969 9 | 0.941 8 | 0.972 1 | 0.967 6 | 0.962 8 | 0.962 4 |
LB | 0.026 4 | 0.042 8 | 0.036 5 | **** | 0.979 3 | 0.983 3 | 0.980 7 | 0.990 2 | 0.980 0 | 0.974 3 |
FY | 0.045 8 | 0.039 0 | 0.030 5 | 0.020 9 | **** | 0.969 4 | 0.979 3 | 0.968 1 | 0.977 6 | 0.966 8 |
SB | 0.081 5 | 0.060 6 | 0.059 9 | 0.016 9 | 0.031 1 | **** | 0.981 0 | 0.965 3 | 0.970 7 | 0.928 4 |
XSBN | 0.058 3 | 0.025 0 | 0.028 3 | 0.019 5 | 0.020 9 | 0.019 2 | **** | 0.979 3 | 0.989 5 | 0.953 8 |
YNPE | 0.022 6 | 0.036 1 | 0.033 0 | 0.009 8 | 0.032 4 | 0.035 3 | 0.021 0 | **** | 0.987 2 | 0.975 6 |
HL | 0.039 0 | 0.041 8 | 0.037 9 | 0.020 2 | 0.022 7 | 0.029 7 | 0.010 5 | 0.012 9 | **** | 0.968 7 |
LS | 0.009 1 | 0.056 6 | 0.038 3 | 0.026 0 | 0.033 7 | 0.074 3 | 0.047 3 | 0.024 8 | 0.031 8 | **** |
图1 麻疯树种群的Nei’s遗传距离UPGMA聚类图。种群代号同表1。
Fig. 1 UPGMA dendrogram of Jatropha curcas populations based on Nei’s genetic distance. Population codes see Table 1.
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