Effect of leaf nitrogen allocation on photosynthetic nitrogen use efficiency at flowering and boll stage of Gossypium spp.

doi:10.17521/cjpe.2022.0490

Abstract

Abstract:

Aims The purpose of this study is to investigate the relationship between net photosynthetic rate (P_n) and nitrogen allocation in Gossypium spp. leaves, to analyze the limiting factors that affect the improvement of photosynthetic nitrogen use efficiency (PNUE), and to reveal the photosynthetic physiological regulation mechanism of nitrogen use efficiency.

Methods In this study, the gas exchange parameters, nitrogen content and proportion of different components, cell wall content per unit area (CW_area) of 16 genotypes of Gossypium spp., including G. hirsutum, G. barbadense, G. arboreum and G. herbaceum, were measured at flowering and bolling stages. The relationship between P_n,CW_area, PNUE and nitrogen content of different components was analyzed.

Important findings The results showed that when nitrogen content per unit area (N_area) was less than 3.75 g·m^-2, the P_n was very significant positively correlated with N_area, When N_area exceeds this value, the P_n was not correlated with N_area, while P_n had a significant positive correlation with Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) nitrogen content (N_r) and bioenergetics nitrogen content (N_b), a marginal positive correlation with light harvesting system nitrogen content (N_L) in photosynthetic apparatus, indicating the nitrogen content of photosynthetic apparatus (N_p) plays more important role than N_area in the photosynthetic capacity. In addition, P_n was also positively correlated with stomatal conductance (G_s). The low P_n of genotype ‘CHANG ZI 1 HAO’ was mainly related to low N_p, while low P_n of genotype ‘MOC-620’ was mainly related to low G_s. The proportion of nitrogen components in photosynthetic apparatus was also significantly and inversely correlated with PNUE. However, the fractions of nitrogen allocated to photosynthetic apparatus components were affected by N_area and CW_area. The correlation analysis showed that the fraction of nitrogen allocation to bioenergetics (P_b) and light harvesting system (P_L) was inversely correlated with N_area; the fraction of nitrogen allocation to Rubisco (P_r), P_L and P_b were significantly, extremely significant and marginally negatively correlated with CW_area, respectively. The results showed that with the increase of N_area and/or CW_area, leaves tended to allocate nitrogen to non-photosynthetic apparatus, including stored nitrogen component and cell wall component. In addition, cell wall may reduce PNUE by influencing mesophyll conductance (g_m). It’s likely to improve the photosynthetic nitrogen use efficiency in genotype ‘CHANG ZI 1 HAO’ and ‘MOC-620’ by increasing the fraction of N allocation to photosynthetic apparatus and decreasing allocation to non-photosynthetic apparatus.

Key words: net photosynthetic rate, nitrogen components of photosynthetic apparatus, photosynthetic nitrogen use efficiency, cell wall content per unit area, Gossypium spp.

FENG Xu-Fei, LEI Zhang-Ying, ZHANG Yu-Jie, XIANG Dao, YANG Ming-Feng, ZHANG Wang-Feng, ZHANG Ya-Li. Effect of leaf nitrogen allocation on photosynthetic nitrogen use efficiency at flowering and boll stage of Gossypium spp.[J]. Chin J Plant Ecol, 2023, 47(11): 1600-1610.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2022.0490

https://www.plant-ecology.com/EN/Y2023/V47/I11/1600

Figures/Tables 9

Table 1 16 genotypes of Gossypium spp. in this study

序号 Serial number	种 Species	基因型 Genotype
1	陆地棉 G. hirsutum	GP84
2	陆地棉 G. hirsutum	陆92-3 LU 92-3
3	陆地棉 G. hirsutum	切尔盘111 chirpan111
4	陆地棉 G. hirsutum	绉缩叶(vin5) ZHOU SUO YE (vin5)
5	海岛棉 G. barbadense	苏联B9103 SU LIAN B9103
6	海岛棉 G. barbadense	MOC-620
7	海岛棉 G. barbadense	新海5号 XIN HAI 5 HAO
8	海岛棉 G. barbadense	新海34号 XIN HAI 34 HAO
9	海岛棉 G. barbadense	新海36号 XIN HAI 36 HAO
10	树棉 G. arboreum	常紫1号 CHANG ZI 1 HAO
11	树棉 G. arboreum	江宁紫花毛籽 JIANG NING ZI HUA MAO ZI
12	树棉 G. arboreum	119S
13	树棉 G. arboreum	白密拉黄花黑籽 BAI MI LA HUANG HUA HEI ZI
14	树棉 G. arboreum	小毛籽 XIAO MAO ZI
15	草棉 G. herbaceum	中草1号 ZHONG CAO 1 HAO
16	草棉 G. herbaceum	金塔草棉 JIN TA CAO MIAN

Fig. 1 Histogram of frequency distribution in the net photosynthetic rate (Pn) and nitrogen content per unit leaf area (Narea) of 16 genotypes of Gossypium spp.

Table 2 Net photosynthetic rate (Pn), nitrogen content per unit area (Narea), Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) nitrogen content (Nr), bioenergetics nitrogen content (Nb), light harvesting system nitrogen content (NL) and photosynthetic apparatus nitrogen content (Np) of 16 genotypes of Gossypium spp. (mean ± SD)

基因型 Genotype	P_n (μmol·m^-2·s^-1)	N_area (g·m^-2)	N_r (g·m^-2)	N_b (g·m^-2)	N_L (g·m^-2)	N_p (g·m^-2)
GP84	35.01 ± 1.280	3.679 ± 0.055	2.218 ± 0.093	0.232 ± 0.016	0.224 ± 0.006	2.675 ± 0.109
陆92-3 LU 92-3	34.79 ± 1.069	3.554 ± 0.111	1.529 ± 0.001	0.230 ± 0.017	0.214 ± 0.008	1.973 ± 0.007
切尔盘111 chirpan111	31.42 ± 0.857	2.988 ± 0.061	1.138 ± 0.033	0.157 ± 0.001	0.206 ± 0.002	1.500 ± 0.048
绉缩叶(vin5) ZHOU SUO YE (vin5)	39.50 ± 0.863	3.720 ± 0.042	1.535 ± 0.045	0.218 ± 0.005	0.243 ± 0.003	1.996 ± 0.090
苏联B9103 SU LIAN B9103	34.67 ± 0.960	3.320 ± 0.004	1.584 ± 0.065	0.202 ± 0.008	0.216 ± 0.001	2.002 ± 0.061
MOC-620	30.65 ± 0.782	4.718 ± 0.107	1.816 ± 0.058	0.191 ± 0.005	0.240 ± 0.004	2.247 ± 0.065
新海5号 XIN HAI 5 HAO	33.55 ± 0.670	3.336 ± 0.031	1.439 ± 0.028	0.188 ± 0.004	0.219 ± 0.005	1.846 ± 0.036
新海34号 XIN HAI 34 HAO	33.28 ± 0.792	3.249 ± 0.073	1.514 ± 0.042	0.200 ± 0.004	0.246 ± 0.009	1.961 ± 0.041
新海36号 XIN HAI 36 HAO	38.34 ± 1.853	3.014 ± 0.042	1.423 ± 0.103	0.196 ± 0.014	0.221 ± 0.011	1.840 ± 0.126
常紫1号 CHANG ZI 1 HAO	28.96 ± 1.899	3.968 ± 0.046	1.192 ± 0.064	0.163 ± 0.003	0.191 ± 0.006	1.546 ± 0.070
江宁紫花毛籽 JIANG NING ZI HUA MAO ZI	29.30 ± 1.131	3.260 ± 0.044	1.317 ± 0.105	0.197 ± 0.016	0.191 ± 0.005	1.705 ± 0.127
119S	33.03 ± 0.400	2.834 ± 0.030	1.420 ± 0.062	0.218 ± 0.012	0.182 ± 0.008	1.820 ± 0.066
白密拉黄花黑籽 BAI MI LA HUANG HUA HEI ZI	25.04 ± 0.171	2.623 ± 0.039	0.980 ± 0.076	0.171 ± 0.003	0.201 ± 0.004	1.352 ± 0.077
小毛籽 XIAO MAO ZI	28.91 ± 1.065	2.483 ± 0.027	1.131 ± 0.091	0.185 ± 0.011	0.162 ± 0.005	1.477 ± 0.102
中草1号 ZHONG CAO 1 HAO	33.85 ± 0.918	3.117 ± 0.033	1.586 ± 0.070	0.207 ± 0.002	0.175 ± 0.003	1.968 ± 0.067
金塔草棉 JIN TA CAO MIAN	32.78 ± 0.716	3.171 ± 0.056	1.349 ± 0.099	0.216 ± 0.009	0.199 ± 0.005	1.764 ± 0.107

基因型 Genotype	P_n (μmol·m^-2·s^-1)	N_area (g·m^-2)	N_r (g·m^-2)	N_b (g·m^-2)	N_L (g·m^-2)	N_p (g·m^-2)
GP84	35.01 ± 1.280	3.679 ± 0.055	2.218 ± 0.093	0.232 ± 0.016	0.224 ± 0.006	2.675 ± 0.109
陆92-3 LU 92-3	34.79 ± 1.069	3.554 ± 0.111	1.529 ± 0.001	0.230 ± 0.017	0.214 ± 0.008	1.973 ± 0.007
切尔盘111 chirpan111	31.42 ± 0.857	2.988 ± 0.061	1.138 ± 0.033	0.157 ± 0.001	0.206 ± 0.002	1.500 ± 0.048
绉缩叶(vin5) ZHOU SUO YE (vin5)	39.50 ± 0.863	3.720 ± 0.042	1.535 ± 0.045	0.218 ± 0.005	0.243 ± 0.003	1.996 ± 0.090
苏联B9103 SU LIAN B9103	34.67 ± 0.960	3.320 ± 0.004	1.584 ± 0.065	0.202 ± 0.008	0.216 ± 0.001	2.002 ± 0.061
MOC-620	30.65 ± 0.782	4.718 ± 0.107	1.816 ± 0.058	0.191 ± 0.005	0.240 ± 0.004	2.247 ± 0.065
新海5号 XIN HAI 5 HAO	33.55 ± 0.670	3.336 ± 0.031	1.439 ± 0.028	0.188 ± 0.004	0.219 ± 0.005	1.846 ± 0.036
新海34号 XIN HAI 34 HAO	33.28 ± 0.792	3.249 ± 0.073	1.514 ± 0.042	0.200 ± 0.004	0.246 ± 0.009	1.961 ± 0.041
新海36号 XIN HAI 36 HAO	38.34 ± 1.853	3.014 ± 0.042	1.423 ± 0.103	0.196 ± 0.014	0.221 ± 0.011	1.840 ± 0.126
常紫1号 CHANG ZI 1 HAO	28.96 ± 1.899	3.968 ± 0.046	1.192 ± 0.064	0.163 ± 0.003	0.191 ± 0.006	1.546 ± 0.070
江宁紫花毛籽 JIANG NING ZI HUA MAO ZI	29.30 ± 1.131	3.260 ± 0.044	1.317 ± 0.105	0.197 ± 0.016	0.191 ± 0.005	1.705 ± 0.127
119S	33.03 ± 0.400	2.834 ± 0.030	1.420 ± 0.062	0.218 ± 0.012	0.182 ± 0.008	1.820 ± 0.066
白密拉黄花黑籽 BAI MI LA HUANG HUA HEI ZI	25.04 ± 0.171	2.623 ± 0.039	0.980 ± 0.076	0.171 ± 0.003	0.201 ± 0.004	1.352 ± 0.077
小毛籽 XIAO MAO ZI	28.91 ± 1.065	2.483 ± 0.027	1.131 ± 0.091	0.185 ± 0.011	0.162 ± 0.005	1.477 ± 0.102
中草1号 ZHONG CAO 1 HAO	33.85 ± 0.918	3.117 ± 0.033	1.586 ± 0.070	0.207 ± 0.002	0.175 ± 0.003	1.968 ± 0.067
金塔草棉 JIN TA CAO MIAN	32.78 ± 0.716	3.171 ± 0.056	1.349 ± 0.099	0.216 ± 0.009	0.199 ± 0.005	1.764 ± 0.107

Fig. 2 Correlation between net photosynthetic rate (Pn) and nitrogen content per unit leaf area (Narea) for 16 genotypes of Gossypium spp. The small figure in the upper right corner shows the correlation between Pn and Narea for genotypes with Narea less than 3.75 g·m-2. Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

Fig. 3 Correlation between nitrogen content of different components in photosynthetic apparatus and net photosynthetic rate (Pn) of Gossypium spp.. A, Photosynthetic apparatus nitrogen content (Np). B, Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) nitrogen content (Nr). C, Bioenergetics nitrogen content (Nb). D, Light harvesting system nitrogen content (NL). Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

Fig. 4 Correlation between the bioenergetics nitrogen content (Nb), light harvesting system nitrogen content (NL) and Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) nitrogen content (Nr) of Gossypium spp. Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

Fig. 5 Correlation between the fraction of nitrogen allocation to different components and photosynthetic nitrogen use efficiency (PNUE) of Gossypium spp. A, Fraction of nitrogen allocation to photosynthetic apparatus (Pp). B, Fraction of nitrogen allocation to Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (Pr). C, Fraction of nitrogen allocation to bioenergetics (Pb). D, Fraction of nitrogen allocation to light-harvesting components (PL). E, Fraction of nitrogen allocation to non-photosynthetic apparatus (Pother). Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

Fig. 6 Correlation between the fraction of nitrogen allocation to different components and nitrogen content per unit area (Narea) of Gossypium spp. A, Fraction of nitrogen allocation to Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (Pr). B, Fraction of nitrogen allocation to bioenergetics (Pb). C, Fraction of nitrogen allocation to light-harvesting components (PL). Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

Fig. 7 Correlation between the cell wall content per unit area (CWarea) and the fraction of nitrogen allocation to different photosynthetic apparatus component and the net photosynthetic rate (Pn) of Gossypium spp. A, Fraction of nitrogen allocation to Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (Pr). B, Fraction of nitrogen allocation to light-harvesting component (PL). C, Fraction of nitrogen allocation to bioenergetics (Pb). D, Net photosynthetic rate (Pn). E, Photosynthetic nitrogen use efficiency (PNUE). Red dot and blue dot indicate genotypes with Narea greater than 3.75 g·m-2. The red dot represents the genotype ‘МОС-620’, and the blue dot represents the genotype ‘CHANG ZI 1 HAO’.

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