Effects of Zn treatment on physiological characteristics, photosynthesis, ultrastructure of root tip cell and grain Zn accumulation in different varieties of rape

doi:10.3724/SP.J.1258.2012.01082

Abstract

Abstract:

Aims Our objectives were to reveal differences in physiological characteristics, photosynthesis, ultrastructure of root tip cell and grain Zinc (Zn) accumulation in different varieties of rape under different Zn treatments.
Methods Pot experiments were carried out. Zn treatments were 0, 1.0, 5.0, 10.0 and 20.0 mg·kg ^-1, and five varieties of rape were ‘Luopingjincaizi’ (Brassica juncea), ‘Erniuwei’ (B. juncea), ‘Liyangkucai’ (B. juncea), ‘Nantonghuangyoucai’ (B. chinensis) and ‘H33’ (B. napus). All experiments were performed in triplicate, and pots were arranged in a completely randomized design inside a greenhouse.
Important findings The dry weights of roots, leaf, straw, grain and plant, physiological characteristics and concentration and accumulation of Zn significantly differed among the different varieties and Zn treatments. The activities of antioxidant enzymes, net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r), and dry weights of plant, straw, leaf and grain increased at Zn ≤ 5.0 mg·kg ^-1. The highest yields of grain for the five varieties were all observed in the 5.0 mg·kg ^-1 Zn treatment and were 37.7%, 23.4%, 29.5%, 82.6% and 18.0% greater than the control, respectively. Ultrastructural damage was found in the root-tip cells for ‘Luopingjincaizi’, ‘Erniuwei’, ‘Nantonghuangyoucai’ and ‘Liyangkucai’ in the presence of 20.0 mg·kg ^-1 Zn and included mitochondria inflation, cell-wall thickening, nucleus atrophy and decreased contents of nucleus, while unaltered structure of the root-tip cells was observed in ‘H33’. Grain Zn accumulation for the five varieties increased at Zn ≤ 5.0 mg·kg ^-1, and decreased at Zn > 5.0 mg·kg ^-1. Highest concentration and accumulation of Zn in grains were observed in ‘Erniuwei’ at 5.0 and 10.0 mg·kg ^-1 Zn (172.34 mg·kg ^-1, 2.932 mg·pot ^-1 and 164.10 mg·kg ^-1, 2.575 mg·pot ^-1) treatment, respectively.

Key words: grain Zn accumulation, physiological characteristic, rape (Brassica), ultrastructure of root tip cell

LIU Jun, CHEN Gui-Qing, XU Wei-Hong, HAN Gui-Qi, ZHANG Hai-Bo, WANG Hui-Xian, ZHANG Ming- Zhong. Effects of Zn treatment on physiological characteristics, photosynthesis, ultrastructure of root tip cell and grain Zn accumulation in different varieties of rape[J]. Chin J Plant Ecol, 2012, 36(10): 1082-1094.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2012.01082

https://www.plant-ecology.com/EN/Y2012/V36/I10/1082

Figures/Tables 7

References 34

1	Aziz EE, El-Din AAE, Omer EA ( 2010). Influence of zinc and iron on plant growth and chemical constituents of Cymbopogon citratus L. grown in newly in reclaimed land. International Journal of Academic Research, 2, 278-283.
2	Cakmak I ( 1996). A NATO-funded project: improvement of Zinc nutritional status of plants and humans in Turkey through plant breeding. Micronutrients and Agriculture, ( 1), 27-28.
3	Chang YX ( 常云霞), Wang HX ( 王红星), Chen L ( 陈龙 ) ( 2011). Salicylic acid mitigating the inhibition of zinc stress to wheat seedling growth. Acta Botanica Boreali- Occidentalia Sinica (西北植物学报), 31, 2052-2056. (in Chinese with English abstract)
4	Epstein E (1983). Crop tolerance of salinity and other mineral stresses. In: Nugent J, O’Conner M eds. Ciba Foundation Symposium97-Better Crops for Food. Pitman, London. 61-68.
5	Farquhar GD, Sharkey TD ( 1982). Stomatal conductance and photosynthesis. Annual Review of Plant Physiology, 33, 317-345.
6	Graham RD ( 1996). CGIAR micronutrients project: results of initial germplasm screening for food staples. Micronutrients and Agriculture, ( 1), 15-16.
7	Graham RD, Senadhira D, Beebe S, Iglesias C, Monasterio I ( 1999). Breeding for micronutrient density in edible portions of staple food crops: conventional approaches. Field Crops Research, 60, 57-80.
8	Han JL ( 韩金玲 ) ( 2003). Effects of Zinc on Photosynthetic Performance, Grain Yield and Quality in Winter Wheat (锌对冬小麦光合特性及产量和品质的影响). Master degree dissertation, Hebei Agriculture University,Baoding, Hebei. 15-16. (in Chinese with English abstract)
9	Hou LP ( 侯雷平), Wu JH ( 吴俊华), Li ML ( 李梅兰), Li YX ( 李远新 ) ( 2010). Effects of Zn fertilization on antioxidative capacity and flavor quality of tomato fruit. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 16, 763-767. (in Chinese with English abstract)
10	Hu XY ( 胡学玉), Li XH ( 李学垣), Xie ZC ( 谢振翅 ) ( 2001). Study on characteristics of Zn efficiency of various pakchoi cultivars. Scientia Agricultura Sinica (中国农业科学), 34, 227-231. (in Chinese with English abstract)
11	Jain R, Srivastava S, Solomon S, Shrivastava AK, Chandra A ( 2010). Impact of excess zinc on growth parameters, cell division, nutrient accumulation, photosynthetic pigments and oxidative stress of sugarcane ( Saccharum spp.). Acta Physiologiae Plantarum, 32, 979-986.
12	Jiang LL ( 姜丽丽), Li XH ( 李絮花), Wang C ( 王川), Li W ( 李伟), Li HY ( 李海燕 ) ( 2011). Effects of combined application of nitrogen, phosphorus, potassium and zinc fertilizer on the physiological metabolism of flag leaf on wheat. Chinese Agricultural Science Bulletin (中国农学通报), 27(15), 57-61. (in Chinese with English abstract)
13	Jie MH ( 颉敏华), Zhang JS ( 张继澍), Jie JM ( 颉建明), Yu JH ( 郁继华 ) ( 2008). Effects of Zinc on photosynthetic characteristics in leaves of Brassica oleracea L. Vat Italicap. Journal of Lanzhou University (Natural Sciences) (兰州大学学报(自然科学版)), 44(5), 35-39. (in Chinese with English abstract)
14	Li HS ( 李合生 ) (2000). Experiments of Plant Physiology and Biochemistry (植物生理生化实验). High Education Press, Beijing. 165-167. (in Chinese)
15	Li WY ( 李文一), Xu WH ( 徐卫红), Hu XF ( 胡小凤), Li YR ( 李仰锐), Liu JZ ( 刘吉振), Wang HX ( 王宏信), Xiong ZT ( 熊治廷 ) ( 2007). Effects of zinc stress on growth, physiological and biochemical and Zn uptake of ryegrass (Lolium perenne L.). Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 23(5), 190-194. (in Chinese with English abstract)
16	Li ZG ( 李志刚), Ye ZQ ( 叶正钱), Fang YY ( 方云英), Yang XE ( 杨肖娥 ) ( 2003). Effect of zinc on plant growth and zinc partitioning in rice plant. Chinese Journal of Rice Science (中国水稻科学), 17, 61-66. (in Chinese with English abstract)
17	Lu RK ( 鲁如坤 ) (2002). Agricultural Chemical Analysis of Soil (土壤农业化学分析方法). China Agricultural Science and Technology Press, Beijing. 24-214. (in Chinese)
18	Lu XC ( 陆欣春), Tian XH ( 田霄鸿), Yang XW ( 杨习文), Mai WX ( 买文选), Zhao AQ ( 赵爱青), Bao QL ( 保琼莉 ) ( 2010). Effect of combination use of Zn and N fertilizers on yield and Zn content in winter wheat. Chinese Journal of Eco-Agriculture (中国生态农业学报), 18, 923-928. (in Chinese with English abstract)
19	Ou ZY ( 欧志英), Peng CL ( 彭长连), Yang CW ( 阳成伟), Lin GZ ( 林桂珠), Duan J ( 段俊), Wen X ( 温学 ) ( 2003). High efficiency photosynthetic characteristics in flag leaves of super high-yielding rice. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 11, 1-6. (in Chinese with English abstract)
20	Qiu CE ( 邱昌恩), Bi YH ( 毕永红), Hu ZY ( 胡征宇 ) ( 2007). The effects of Zn ²+ stress on the growth, physiological characteristics and cell structure of Chlorococcum sp . Acta Hydrobiologica Sinica (水生生物学报), 31, 503-508. (in Chinese with English abstract)
21	Qu YN, Zhou Q, Yu BJ ( 2009). Effects of Zn ²+ and niflumic acid on photosynthesis in Glycine soja and Glycine max seedling under NaCl stress. Environmental and Experimental Botany, 65, 304-309.
22	Sharma PN, Tripathi A, Bisht SS ( 1995). Zinc requirement for stomatal opening in cauliflower. Plant Physiology, 107, 751-756. DOI URL PMID
23	Tang YT ( 汤叶涛), Wu YD ( 吴妤都), Qiu RL ( 仇荣亮), Zeng XW ( 曾晓雯), Hu PJ ( 胡鹏杰 ) ( 2009). Zinc uptake and accumulation in Picris divaricata Vant. Acta Ecologica Sinica (生态学报), 29, 1823-1831. (in Chinese with English abstract)
24	Wang H ( 汪洪), Jin JY ( 金继运 ) ( 2009). The physiological and molecular mechanisms of zinc uptake, transport, and hyperaccumulation in plants: a review. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 15, 225-235. (in Chinese with English abstract)
25	Wang HX ( 王慧先), Guo JY ( 郭俊云), Xu WH ( 徐卫红), Zhang HB ( 张海波), Chen GQ ( 陈贵青), Zhang XJ ( 张晓璟), Zhao J ( 赵静), Wang ZY ( 王正银 ) ( 2011). Response and zinc use efficiency of Chinese cabbage under zinc fertilization. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 17, 154-159. (in Chinese with English abstract)
26	Wang RM ( 王人民), Yang XE ( 杨肖娥), Yang YA ( 杨玉爱 ) ( 1997). Studies on the genotypic differences and its physiological characteristics of rice responses to different Zn ²+ activities . Journal of Zhejiang Agricultural University (浙江农业大学学报), 23, 442-446. (in Chinese with English abstract)
27	Wang SY ( 王淑英), Yu TQ ( 于同泉), Wang JL ( 王建立), Yang L ( 杨柳), Yang K ( 杨凯), Lu P ( 路苹 ) ( 2008). Preliminary study on spatial variability and distribution of soil available microelements in Pinggu District of Beijing. Scientia Agricultura Sinica (中国农业科学), 41, 129-137. (in Chinese with English abstract)
28	Wei XR ( 魏孝荣), Hao MD ( 郝明德), Zhang CX ( 张春霞), Wang XG ( 王旭刚 ) ( 2005). Effects of zinc and manganese fertilizers on maize photosynthetic performance under soil drought condition. Acta Agronomica Sinica (作物学报), 31, 1101-1104. (in Chinese with English abstract)
29	Xu CL ( 徐春丽), Xiao JX ( 肖家欣), Qi XX ( 齐笑笑), Zhang SL ( 张绍铃 ) ( 2010). Effect of Zn stress on photosynthetic characteristic and their correlation of the two varieties of Citrus. Journal of Biology (生物学杂志), 27(6), 42-45. (in Chinese with English abstract)
30	Xu QS ( 徐勤松), Shi GX ( 施国新), Du KH ( 杜开和 ) ( 2001). Studies on correlation between peroxidation damage by free radicals and ultrastructural changes of mesophyll cells in Ottelia alismoides (L.) Pers induced by Zn pollution. Bulletin of Botany (植物学通报), 18, 597-604. (in Chinese with English abstract)
31	Xu QS ( 徐勤松), Shi GX ( 施国新), Wang X ( 王学), Dingt BZ ( 丁秉中), Chen SY ( 陈苏雅), Xu BJ ( 许丙军), Zhao J ( 赵娟 ) ( 2006). Subcellular localization of Pb in cells of Eichhornia crassipes Solms by electron microscopy. Journal of Nanjing Normal University (Natural Science Edition) (南京师大学报(自然科学版)), 29, 81-58. (in Chinese with English abstract)
32	Xu WH, Huang H, Xiong ZT ( 2005). Effects of Zn stress on Zn accumulation and anti-oxidant enzymes activity in four varieties of ryegrass. Wuhan University Journal of Natural Sciences, 10, 1051-1056.
33	Yang JH ( 杨剑虹), Wang CQ ( 王成秋), Dai HL ( 代享林 ) (2008). Soil Agricultural Chemical Analysis and Environmental Monitoring (土壤农化分析与环境监测). China Land Press, Beijing. 16-310(in Chinese)
34	Zhang ZL ( 张志良), Wang YD ( 王英典), Liu N ( 刘宁 ) (2002). Experimental Guidance for Plant Biology (植物生物学实验指导). High Education Press, Beijing. 154-155. (in Chinese)

	Zn处理 Zn treatment (mg·kg^-1)	茎 Stem	叶 Leaf	根 Root	籽粒 Grain	总干重 Total dry weight
品种 Variety
‘罗平金菜子’ ‘Luopingjincaizi’	0	15.77 ± 0.58	2.15 ± 0.13	2.06 ± 0.04	12.31 ± 0.33	32.29 ± 0.22
	1	16.82 ± 0.73	2.57 ± 0.09	2.78 ± 0.07	14.00 ± 0.35	36.17 ± 0.30
	5	18.02 ± 0.66	3.77 ± 0.11	3.69 ± 0.09	16.95 ± 0.27	42.43 ± 0.49
	10	17.23 ± 0.50	2.32 ± 0.12	2.55 ± 0.02	15.73 ± 0.13	37.83 ± 0.37
	20	14.31 ± 0.42	2.01 ± 0.08	2.01 ± 0.03	14.28 ± 0.21	32.61 ± 0.32
‘二牛尾’ ‘Erniuwei’	0	32.41 ± 0.81	8.76 ± 0.25	3.11 ± 0.11	13.78 ± 0.26	58.06 ± 0.65
	1	37.14 ± 0.67	9.33 ± 0.27	3.97 ± 0.08	14.01 ± 0.22	64.45 ± 0.61
	5	39.88 ± 0.73	10.79 ± 0.31	5.01 ± 0.12	17.01 ± 0.46	72.69 ± 0.78
	10	35.17 ± 0.49	10.11 ± 0.26	4.37 ± 0.15	15.69 ± 0.53	65.34 ± 0.69
	20	29.36 ± 0.38	9.17 ± 0.19	2.99 ± 0.10	10.59 ± 0.29	52.11 ± 0.39
‘溧阳苦菜’ ‘Liyangkucai’	0	37.26 ± 0.75	10.28 ± 0.34	2.58 ± 0.09	12.63 ± 0.26	62.75 ± 0.61
	1	41.55 ± 0.82	13.11 ± 0.25	3.02 ± 0.08	13.33 ± 0.30	71.01 ± 0.86
	5	43.12 ± 0.91	15.47 ± 0.23	3.47 ± 0.16	16.35 ± 0.43	78.41 ± 0.54
	10	40.88 ± 0.65	14.79 ± 0.35	2.52 ± 0.05	10.95 ± 0.17	69.14 ± 0.42
	20	35.17 ± 0.58	11.25 ± 0.31	2.59 ± 0.07	10.51 ± 0.11	59.52 ± 0.35
‘南通黄油菜’ ‘Nantonghuang- youcai’	0	21.11 ± 0.49	2.38 ± 0.07	2.51 ± 0.04	8.94 ± 0.10	34.94 ± 0.29
	1	23.85 ± 0.33	3.96 ± 0.10	2.94 ± 0.05	13.43 ± 0.27	44.18 ± 0.42
	5	28.27 ± 0.25	5.17 ± 0.12	3.18 ± 0.12	16.32 ± 0.39	52.94 ± 0.48
	10	25.97 ± 0.29	4.63 ± 0.15	3.67 ± 0.10	10.59 ± 0.15	44.86 ± 0.25
	20	22.69 ± 0.36	2.02 ± 0.05	4.21 ± 0.19	10.45 ± 0.14	39.37 ± 0.31
‘H33’	0	26.34 ± 0.24	3.17 ± 0.11	1.58 ± 0.05	14.98 ± 0.28	46.07 ± 0.29
	1	27.91 ± 0.41	4.79 ± 0.17	1.82 ± 0.03	16.22 ± 0.32	50.74 ± 0.35
	5	30.27 ± 0.30	5.21 ± 0.23	2.34 ± 0.07	17.68 ± 0.36	55.5 ± 0.39
	10	28.89 ± 0.25	4.07 ± 0.14	2.07 ± 0.09	14.08 ± 0.34	49.11 ± 0.45
	20	27.21 ± 0.17	3.34 ± 0.26	2.01 ± 0.14	13.06 ± 0.30	45.62 ± 0.36
p
品种 Variety		<0.001	<0.001	0.003	0.005	<0.001
Zn处理 Zn treatment		<0.001	<0.001	<0.001	<0.001	<0.001
品种× Zn处理 Variety × Zn treatment		<0.001	<0.001	<0.001	<0.001	<0.001

	Zn处理 Zn treatment (mg·kg^-1)	茎 Stem	叶 Leaf	根 Root	籽粒 Grain	总干重 Total dry weight
品种 Variety
‘罗平金菜子’ ‘Luopingjincaizi’	0	15.77 ± 0.58	2.15 ± 0.13	2.06 ± 0.04	12.31 ± 0.33	32.29 ± 0.22
	1	16.82 ± 0.73	2.57 ± 0.09	2.78 ± 0.07	14.00 ± 0.35	36.17 ± 0.30
	5	18.02 ± 0.66	3.77 ± 0.11	3.69 ± 0.09	16.95 ± 0.27	42.43 ± 0.49
	10	17.23 ± 0.50	2.32 ± 0.12	2.55 ± 0.02	15.73 ± 0.13	37.83 ± 0.37
	20	14.31 ± 0.42	2.01 ± 0.08	2.01 ± 0.03	14.28 ± 0.21	32.61 ± 0.32
‘二牛尾’ ‘Erniuwei’	0	32.41 ± 0.81	8.76 ± 0.25	3.11 ± 0.11	13.78 ± 0.26	58.06 ± 0.65
	1	37.14 ± 0.67	9.33 ± 0.27	3.97 ± 0.08	14.01 ± 0.22	64.45 ± 0.61
	5	39.88 ± 0.73	10.79 ± 0.31	5.01 ± 0.12	17.01 ± 0.46	72.69 ± 0.78
	10	35.17 ± 0.49	10.11 ± 0.26	4.37 ± 0.15	15.69 ± 0.53	65.34 ± 0.69
	20	29.36 ± 0.38	9.17 ± 0.19	2.99 ± 0.10	10.59 ± 0.29	52.11 ± 0.39
‘溧阳苦菜’ ‘Liyangkucai’	0	37.26 ± 0.75	10.28 ± 0.34	2.58 ± 0.09	12.63 ± 0.26	62.75 ± 0.61
	1	41.55 ± 0.82	13.11 ± 0.25	3.02 ± 0.08	13.33 ± 0.30	71.01 ± 0.86
	5	43.12 ± 0.91	15.47 ± 0.23	3.47 ± 0.16	16.35 ± 0.43	78.41 ± 0.54
	10	40.88 ± 0.65	14.79 ± 0.35	2.52 ± 0.05	10.95 ± 0.17	69.14 ± 0.42
	20	35.17 ± 0.58	11.25 ± 0.31	2.59 ± 0.07	10.51 ± 0.11	59.52 ± 0.35
‘南通黄油菜’ ‘Nantonghuang- youcai’	0	21.11 ± 0.49	2.38 ± 0.07	2.51 ± 0.04	8.94 ± 0.10	34.94 ± 0.29
	1	23.85 ± 0.33	3.96 ± 0.10	2.94 ± 0.05	13.43 ± 0.27	44.18 ± 0.42
	5	28.27 ± 0.25	5.17 ± 0.12	3.18 ± 0.12	16.32 ± 0.39	52.94 ± 0.48
	10	25.97 ± 0.29	4.63 ± 0.15	3.67 ± 0.10	10.59 ± 0.15	44.86 ± 0.25
	20	22.69 ± 0.36	2.02 ± 0.05	4.21 ± 0.19	10.45 ± 0.14	39.37 ± 0.31
‘H33’	0	26.34 ± 0.24	3.17 ± 0.11	1.58 ± 0.05	14.98 ± 0.28	46.07 ± 0.29
	1	27.91 ± 0.41	4.79 ± 0.17	1.82 ± 0.03	16.22 ± 0.32	50.74 ± 0.35
	5	30.27 ± 0.30	5.21 ± 0.23	2.34 ± 0.07	17.68 ± 0.36	55.5 ± 0.39
	10	28.89 ± 0.25	4.07 ± 0.14	2.07 ± 0.09	14.08 ± 0.34	49.11 ± 0.45
	20	27.21 ± 0.17	3.34 ± 0.26	2.01 ± 0.14	13.06 ± 0.30	45.62 ± 0.36
p
品种 Variety		<0.001	<0.001	0.003	0.005	<0.001
Zn处理 Zn treatment		<0.001	<0.001	<0.001	<0.001	<0.001
品种× Zn处理 Variety × Zn treatment		<0.001	<0.001	<0.001	<0.001	<0.001

	Zn处理 Zn treatment (mg·kg^-1)	净光合速率 P_n(μmol·m^-2·s^-1)	气孔导度 G_s(mol·m^-2·s^-1)	蒸腾速率 T_r(mmol·m^-2·s^-1)	胞间CO₂浓度 C_i (μmol·mol^-1)
品种 Variety
‘罗平金菜子’ ‘Luopingjincaizi’	0	9.01 ± 0.75	0.204 ± 0.001	4.77 ± 0.12	276 ± 3.11
	1	9.88 ± 0.63	0.215 ± 0.004	5.49 ± 0.17	272 ± 2.19
	5	11.69 ± 0.62	0.246 ± 0.003	5.83 ± 0.21	235 ± 1.13
	10	12.76 ± 0.59	0.255 ± 0.004	6.45 ± 0.13	276 ± 2.05
	20	14.65 ± 0.85	0.258 ± 0.006	7.06 ± 0.19	285 ± 2.21
‘二牛尾’ ‘Erniuwei’	0	10.97 ± 0.54	0.108 ± 0.001	2.86 ± 0.09	199 ± 1.10
	1	15.46 ± 0.73	0.254 ± 0.003	4.49 ± 0.14	228 ± 2.16
	5	17.67 ± 0.36	0.442 ± 0.007	6.65 ± 0.18	236 ± 2.32
	10	14.97 ± 0.84	0.244 ± 0.003	3.83 ± 0.10	257 ± 1.76
	20	10.43 ± 0.32	0.092 ± 0.001	2.01 ± 0.08	271 ± 1.55
‘溧阳苦菜’ ‘Liyangkucai’	0	14.46 ± 0.55	0.375 ± 0.004	6.75 ± 0.25	309 ± 3.37
	1	14.92 ± 0.69	0.399 ± 0.003	6.92 ± 0.27	286 ± 2.49
	5	16.38 ± 0.63	0.427 ± 0.007	6.67 ± 0.21	237 ± 1.31
	10	12.55 ± 0.27	0.258 ± 0.005	3.87 ± 0.16	286 ± 1.09
	20	9.76 ± 0.21	0.153 ± 0.003	2.03 ± 0.12	304 ± 2.67
‘南通黄油菜’ ‘Nantonghuangyoucai’	0	7.99 ± 0.16	0.206 ± 0.002	3.12 ± 0.14	329 ± 2.98
	1	9.64 ± 0.33	0.343 ± 0.005	4.48 ± 0.19	319 ± 3.11
	5	11.53 ± 0.28	0.391 ± 0.004	4.81 ± 0.16	324 ± 2.58
	10	9.16 ± 0.11	0.236 ± 0.001	3.96 ± 0.11	342 ± 3.09
	20	7.21 ± 0.13	0.156 ± 0.002	2.69 ± 0.07	399 ± 3.11
‘H33’	0	13.17 ± 0.25	0.283 ± 0.004	5.79 ± 0.20	308 ± 2.67
	1	14.72 ± 0.34	0.295 ± 0.003	6.21 ± 0.22	297 ± 2.16
	5	16.64 ± 0.58	0.319 ± 0.005	6.97 ± 0.29	285 ± 1.89
	10	15.05 ± 0.45	0.326 ± 0.003	6.75 ± 0.40	295 ± 1.75
	20	14.62 ± 0.38	0.333 ± 0.001	6.69 ± 0.18	315 ± 2.93
p
品种 Variety		0.002	<0.001	<0.001	0.001
Zn处理 Zn treatment		<0.001	<0.001	<0.001	<0.001
品种×Zn处理 Variety × Zn treatment		<0.001	<0.001	<0.001	<0.001

	Zn处理 Zn treatment (mg·kg^-1)	净光合速率 P_n(μmol·m^-2·s^-1)	气孔导度 G_s(mol·m^-2·s^-1)	蒸腾速率 T_r(mmol·m^-2·s^-1)	胞间CO₂浓度 C_i (μmol·mol^-1)
品种 Variety
‘罗平金菜子’ ‘Luopingjincaizi’	0	9.01 ± 0.75	0.204 ± 0.001	4.77 ± 0.12	276 ± 3.11
	1	9.88 ± 0.63	0.215 ± 0.004	5.49 ± 0.17	272 ± 2.19
	5	11.69 ± 0.62	0.246 ± 0.003	5.83 ± 0.21	235 ± 1.13
	10	12.76 ± 0.59	0.255 ± 0.004	6.45 ± 0.13	276 ± 2.05
	20	14.65 ± 0.85	0.258 ± 0.006	7.06 ± 0.19	285 ± 2.21
‘二牛尾’ ‘Erniuwei’	0	10.97 ± 0.54	0.108 ± 0.001	2.86 ± 0.09	199 ± 1.10
	1	15.46 ± 0.73	0.254 ± 0.003	4.49 ± 0.14	228 ± 2.16
	5	17.67 ± 0.36	0.442 ± 0.007	6.65 ± 0.18	236 ± 2.32
	10	14.97 ± 0.84	0.244 ± 0.003	3.83 ± 0.10	257 ± 1.76
	20	10.43 ± 0.32	0.092 ± 0.001	2.01 ± 0.08	271 ± 1.55
‘溧阳苦菜’ ‘Liyangkucai’	0	14.46 ± 0.55	0.375 ± 0.004	6.75 ± 0.25	309 ± 3.37
	1	14.92 ± 0.69	0.399 ± 0.003	6.92 ± 0.27	286 ± 2.49
	5	16.38 ± 0.63	0.427 ± 0.007	6.67 ± 0.21	237 ± 1.31
	10	12.55 ± 0.27	0.258 ± 0.005	3.87 ± 0.16	286 ± 1.09
	20	9.76 ± 0.21	0.153 ± 0.003	2.03 ± 0.12	304 ± 2.67
‘南通黄油菜’ ‘Nantonghuangyoucai’	0	7.99 ± 0.16	0.206 ± 0.002	3.12 ± 0.14	329 ± 2.98
	1	9.64 ± 0.33	0.343 ± 0.005	4.48 ± 0.19	319 ± 3.11
	5	11.53 ± 0.28	0.391 ± 0.004	4.81 ± 0.16	324 ± 2.58
	10	9.16 ± 0.11	0.236 ± 0.001	3.96 ± 0.11	342 ± 3.09
	20	7.21 ± 0.13	0.156 ± 0.002	2.69 ± 0.07	399 ± 3.11
‘H33’	0	13.17 ± 0.25	0.283 ± 0.004	5.79 ± 0.20	308 ± 2.67
	1	14.72 ± 0.34	0.295 ± 0.003	6.21 ± 0.22	297 ± 2.16
	5	16.64 ± 0.58	0.319 ± 0.005	6.97 ± 0.29	285 ± 1.89
	10	15.05 ± 0.45	0.326 ± 0.003	6.75 ± 0.40	295 ± 1.75
	20	14.62 ± 0.38	0.333 ± 0.001	6.69 ± 0.18	315 ± 2.93
p
品种 Variety		0.002	<0.001	<0.001	0.001
Zn处理 Zn treatment		<0.001	<0.001	<0.001	<0.001
品种×Zn处理 Variety × Zn treatment		<0.001	<0.001	<0.001	<0.001

[1]	CHEN Shi-Wei,LIU Min-Xia,JIA Yun,AN Qi,AN Yan-Fei. Community succession and photosynthetic physiological characteristics of pasture plants in a sub-alpine meadow in Gannan, China [J]. Chin J Plan Ecolo, 2015, 39(4): 343-351.
[2]	ZHENG Ya-Ping,XIN Cai-Yun,WANG Cai-Bin,SUN Xiu-Shan,YANG Wei-Qiang,WAN Shu-Bo,ZHENG Yong-Mei,FENG Hao,CHEN Dian-Xu,SUN Xue-Wu,WU Zheng-Feng. Effects of phosphorus fertilizer on root morphology, physiological characteristics and yield in peanut (Arachis hypogaea) [J]. Chin J Plant Ecol, 2013, 37(8): 777-785.
[3]	YANG Peng, XU Xiao. Effects of waterlogging stress on the growth and physiological characteristics of male and female Populus cathayana seedlings [J]. Chin J Plant Ecol, 2012, 36(1): 81-87.
[4]	WANG Qing-Hai, ZHANG Wei, QUE Xiao-E, WU Ju-Ying, ZHANG Guo-An, XIAO Bo. Effects of atrazine residue in water on biomass and physiological characteristics of Scirpus tabernaemontani [J]. Chin J Plant Ecol, 2011, 35(2): 223-231.
[5]	MAI Bo-Ru, ZHENG You-Fei, WU Rong-Jun, LIANG Jun, LIU Xia. Effects of simulated sulfur-rich, nitric-rich and mixed acid rain on the physiology, growth and yield of rape (Brassica napus) [J]. Chin J Plant Ecol, 2010, 34(4): 427-437.
[6]	IU Zhao-Pu, DENG Li-Qun, LIU Ling, QI Chang-Hai, CHEN Ming-Da, XIA Tian-Xiang. PHYSIOLOGICAL CHARACTERISTICS OF HELIANTHUS TUBEROSUS IRRIGATED BY SEAWATER, LAIZHOU COAST, SHANDONG PROVINCE [J]. Chin J Plant Ecol, 2005, 29(3): 474-478.
[7]	LI Qing-Kang, MA Ke-Ping. ADVANCES IN PLANT SUCCESSION ECOPHYSIOLOGY [J]. Chin J Plan Ecolo, 2002, 26(增刊): 9-19.