Effects of atrazine residue in water on biomass and physiological characteristics of Scirpus tabernaemontani

doi:10.3724/SP.J.1258.2011.00223

Abstract

Abstract:

Aims Our objective was to assess the phytotoxicity of atrazine residue in water on the emergent aquatic plant Scirpus tabernaemontani.
Methods We conducted hydroponics experiments to study the effects of atrazine stress on peroxidase (POD) and root activities, malonaldehyde (MDA) and chlorophyll contents, relative water content (RWC) of leaf and fresh weight of S. tabernaemontani. The original dosages of atrazine in the culture solution based on Hoagland solution were 1, 2, 4, 8 and 16 mg·L^-1. Plant samples were collected 15, 30, 45 and 60 days after the onset of the experiment.
Important findings POD and root activities, chlorophyll content, RWC and fresh weight of S. tabernaemontani decreased, and MDA contents increased. All results indicated that elevated concentration of atrazine (>8 mg·L^-1) has significant effects on biomass and physiological characteristics of S. tabernaemontani. With the exception of chlorophyll contents, the harmful effects weakened with prolonged treatment exposure due to degradation of atrazine. There were no significant differences in investigated variables between the 1 mg·L ^-1 atrazine treatment and the control during the growing season.

Key words: atrazine, biomass, physiological characteristic, Scirpus tabernaemontani

WANG Qing-Hai, ZHANG Wei, QUE Xiao-E, WU Ju-Ying, ZHANG Guo-An, XIAO Bo. Effects of atrazine residue in water on biomass and physiological characteristics of Scirpus tabernaemontani [J]. Chin J Plant Ecol, 2011, 35(2): 223-231.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00223

https://www.plant-ecology.com/EN/Y2011/V35/I2/223

Figures/Tables 9

Fig. 1 Effects of atrazine on increment of fresh weight of Scirpus tabernaemontani different cultivation time (mean ± SD). Same letters in same cultivation time mean no significant difference by Duncan’s new multiple range test among treatments at the 0.05 level.

Table 1 Change of atrazine concentration in culture solution in experiment period

初始浓度 Initial atrazine concentration (mg·L^-1)	浓度变化 Change of atrazine concentration (mg·L^-1)
初始浓度 Initial atrazine concentration (mg·L^-1)	15 days	30 days	45 days	60 days
1	0.49	0.29	0.17	0.10
2	1.01	0.57	0.32	0.18
4	1.63	0.84	0.43	0.22
8	2.52	1.14	0.52	0.24
16	7.58	3.29	1.43	0.62

Fig. 2 Effects of atrazine on the root activity of Scripus tabernaemontani in different cultivation time (mean ± SD). Notes see Fig. 1.

Fig. 3 Effects of atrazine on relative water content (RWC) of leaf of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.

Fig. 4 Effects of atrazine on peroxidase (POD) activity of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.

Fig. 5 Effects of atrazine on malonaldehyde (MDA) content of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.

Fig. 6 Effects of atrazine on chlorophyll a content of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.

Fig. 7 Effects of atrazine on chlorophyll a+b content of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.

Table 2 The interrelation among the physiological indexes (y) of Scirpus tabernaemontani and atrazine concentration (x)

生理指标 Physiological indexes (y)	回归方程 Equation	R²	P > F
鲜重 Fresh weight (g)	y = -0.1927x + 18.7870	0.855 5	0.008 2
叶片相对含水量 RWC of leaves (%)	y = -1.2056x + 65.9110	0.774 2	0.020 8
根系活力 Root activity (μg·h^-1·g^-1)	y = -2.5399x + 81.2620	0.969 5	0.000 4
叶绿素含量 Chlorophyll a + b content (mg·g^-1DW)	y = -0.0240x + 0.4401	0.616 7	0.064 2
POD活性 POD activity (Δ470·min^-1·μg^-1·FW)	y = -0.3644x + 8.0227	0.982 1	0.000 1
MDA含量 MDA content (μm·g^-1)	y = 0.0040x + 0.0790	0.672 3	0.045 7

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