Chin J Plant Ecol ›› 2014, Vol. 38 ›› Issue (5): 507-514.DOI: 10.3724/SP.J.1258.2014.00047
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XING Bing-Yu1,ZHU Nan1,ZHANG Hong-Pei1,YANG Xi-Ling2,DONG Juan-E1,*()
Received:
2013-10-09
Accepted:
2014-01-10
Online:
2014-10-09
Published:
2014-05-13
Contact:
DONG Juan-E
XING Bing-Yu,ZHU Nan,ZHANG Hong-Pei,YANG Xi-Ling,DONG Juan-E. Effects of methyl viologen on the antioxidant system in cultured Salvia miltiorrhiza cells[J]. Chin J Plant Ecol, 2014, 38(5): 507-514.
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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2014.00047
Fig. 1 Effects of methyl viologen on the accumulation of H2O2 in cultured Salvia miltiorrhiza cells (mean ± SE). Test materials are S. miltiorrhiza cells in suspension cultures for six days. Different small letters indicate significant differences (p < 0.05).
Fig. 2 Effects of diuron (DCMU) and methyl viologen (MV) on the accumulation of H2O2 in cultured Salvia miltiorrhiza cells (mean ± SE). Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
Fig. 3 Effects of different treatments on the accumulation of malondialdehyde (MDA) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
Fig. 4 Effects of different treatments on the accumulation of glutathione (GSH) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
Fig. 5 Effects of different treatments on the activities of superoxide dismutase (SOD) (A), catalase (CAT) (B), and peroxidase (POD) (C) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
Fig. 6 Effects of different treatments on the isoenzymes spectrum of superoxide dismutase (SOD) (A), catalase (CAT) (B), and peroxidase (POD) (C) in culture Salvia miltiorrhiza cells. 1, distilled water treatment; 2, methyl viologen treatment; 3, diuron treatment; 4, methyl viologen + diuron treatment. a-e, soenzyme bands of SOD, CAT and POD.
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