EFFECTS OF OZONE ON ROOT ACTIVITY, SOLUBLE PROTEIN CONTENT AND ANTIOXIDANT SYSTEM IN ORYZA SATIVA ROOTS

doi:10.3773/j.issn.1005-264x.2009.03.001

Abstract

Abstract:

Aims Our purpose was to investigate the response of soluble protein content, lipid peroxidation and antioxidant system in roots of Oryza sativa to different ozone concentrations.

Methods Rice grown in open top chambers in the field were exposed to four ozone concentrations from June 29 to Sept 30, 2007: charcoal-filtered air (CF, 20 nl·L-1), non-filtered air (NF, 40 nl·L-1) and elevated [O3] (75 and 150 nl·L-1, MO and HO). We determined root activity, contents of soluble protein, malonaldehyde (MDA), hydrogen peroxide (H2O2) and ascorbate (ASA), activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD).

Important findings Relative to CF, elevated [O3] (MO and HO) significantly decreased root activity and soluble protein content and increased MDA and H2O2 contents and CAT and POD activities with elongated ozone exposure. However, SOD activity was increased in the early period and decreased in the late period with elevated [O3]. Furthermore, ASA content did not show significant difference between MO/HO and CF, except in the 9th week in MO treatment. With the exceptions of root activity and soluble protein content in late growing season, there were no significant differences in investigated variables between NF and CF during the growing season. All results indicated that elevated [O3] has significant effects on rice roots, including short supply of strong, active roots with prolonged ozone exposure.

Key words: ozone, rice, soluble protein, antioxidant system

ZHANG Wei-Wei, ZHENG Fei-Xiang, WANG Xiao-Ke, FENG Zhao-Zhong, OUYANG Zhi-Yun. EFFECTS OF OZONE ON ROOT ACTIVITY, SOLUBLE PROTEIN CONTENT AND ANTIOXIDANT SYSTEM IN ORYZA SATIVA ROOTS[J]. Chin J Plant Ecol, 2009, 33(3): 425-432.

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