Response of key parameters of leaf photosynthetic models to increased ozone concentration in four common trees

doi:10.17521/cjpe.2021.0295

Abstract

Abstract:

Aims With fast urbanization in China, ground-level ozone (O₃) has become the major atmospheric pollutant in summer. It was documented that O₃ enters leaves through stomata, inhibits photosynthesis, and alters the carbon and water cycles in terrestrial ecosystems. However, few studies have investigated the key parameters of photosynthetic and stomatal conductance models in response to elevated O₃concentration.
Methods In this study, plants of four common tree species (Camellia sinensis, Acer negundo, Koelreuteria paniculata and Quercus mongolica) were exposed to two O₃ treatments (CF, charcoal-filtered air; E-O₃, ambient air + 60 nmol·mol^-1 O₃) in open top chambers. The effects of elevated O₃ concentration on key parameters of photosynthetic and stomatal conductance models were explored using data from leaf gas exchange measurements.
Important findings Our results indicated that elevated O₃ concentration significantly decreased the light-saturated photosynthesis and mesophyll conductance in the four species. However, species showed distinct responses of the maximum rate of Rubisco carboxylation and the maximum rate of electron transport to elevated O₃ concentration. The response of stomatal conductance to O₃ was also different among species. We found that elevated O₃ concentration significantly increased the slope parameters (g₁) of Q. mongolica and A. negundo; however, the intercept parameter of stomata model in C. sinensis was decreased in A. negundo. The intrinsic water- use efficiency of the four species was negatively correlated with g₁ across O₃ treatments. All in all, elevated O₃ concentration significantly affected key parameters of the photosynthetic and stomatal conductance models. This study could provide the foundation and support for improving the accuracy of terrestrial ecosystem models under elevated O₃concentration.

Key words: ozone, woody plant, photosynthetic model, stomatal conductance model

MA Yan-Ze, YANG Xi-Lai, XU Yan-Sen, FENG Zhao-Zhong. Response of key parameters of leaf photosynthetic models to increased ozone concentration in four common trees[J]. Chin J Plant Ecol, 2022, 46(3): 321-329.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2021.0295

https://www.plant-ecology.com/EN/Y2022/V46/I3/321

Figures/Tables 5

Table 1 Variance analysis results of effects of ozone (O3), species, and their interaction on gas exchange traits (p value)

	臭氧 O₃	树种 Species	臭氧×树种 O₃ × Species
饱和光合速率 A_sat	<0.01	0.02	0.26
气孔导度 g_s	<0.01	<0.01	0.01
胞间CO₂浓度 C_i	0.43	0.05	0.25
内源水分利用效率 iWUE	0.25	0.02	0.08
最大羧化速率 V_cmax	<0.01	0.11	0.04
最大电子传递速率 J_max	<0.01	0.30	<0.01
J_max/V_cmax	0.25	<0.01	0.18
叶肉导度 g_m	<0.01	<0.01	0.06

Fig. 1 Effects of elevated ozone concentration (E-O3) on light-saturated net photosynthesis (Asat)(A), stomatal conductance to H2O (gs)(B), CO2 concentration in the leaf intercellular spaces (Ci)(C), and intrinsic water-use efficiency (iWUE)(D) of four tree species (mean ± SD). Different lowercase letters in the figures indicate significant differences (p < 0.05). CF, charcoal-filtered air.

Fig. 2 Effects of elevated ozone concentration (E-O3) on the maximum rates of Rubisco carboxylation (Vcmax)(A), the maximum rate of ribulose 1,5 bisphosphate regeneration (Jmax)(B), Jmax/Vcmax (C), and mesophyll conductance (gm)(D) of four tree species (mean ± SD). Different lowercase letters in the figures stand for the significant differences (p < 0.05). CF, charcoal-filtered air.

Fig. 3 Relationship between stomatal conductance (gs) and the index of the optimal stomatal conductance (1.6 An/(Ca$\sqrt{\text{VPD}}$)) of four tree species. CF, charcoal-filtered air; E-O3, ambient air + 60 nmol·mol–1 O3. An, leaf photosynthetic rate; Ca, environmental CO2 concentration; VPD, vapor pressure defict.

Fig. 4 Relationship between water use efficiency (iWUE) and the slope parameters of stomata model (g1) of four tree species under different treatments. CF, charcoal-filtered air; E-O3, ambient air + 60 nmol·mol-1 O3.

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