Nitrogen supply mitigates the effects of elevated [O3] on photosynthesis and yield in wheat

doi:10.3724/SP.J.1258.2011.00523

Abstract

Abstract:

Aims Our objective was to study the interactive influences of ozone (O₃) and nitrogen (N) on photosynthesis and yield in winter wheat (Triticum aestivum).

Methods The winter wheat was exposed to two levels of O₃ (ambient and 1.5 ambient) and two levels of N supply (210 and 250 kg·hm^-2) under field conditions.

Important findings O₃ exposure significantly reduced the net photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) of wheat leaves in the filling stage by 28.95%, 31.79 % and 23.17%, respectively. O₃ exposure also significantly reduced the content of chlorophyll a (Chl a), chlorophyll b (Chl b), carotene (Car), total chlorophyll (Chl t) and soluble protein in the filling stage by 58.89%, 68.64%, 22.89%, 60.31% and 32.00%, respectively, while intercellular CO₂ concentration (C_i) changed slightly. Biomass in the maturing stage and yield of the wheat were also reduced by elevated O₃ by 12.23% and 12.63%, respectively. High N availability significantly increased P_n, Chl a, Chl b, soluble protein, biomass and yield of the wheat leaves by 25.66%, 83.05%, 121.57%, 30.33%, 14.94% and 10.67%, respectively, while G_s, C_i, T_r and Car were influenced slightly by high N, which indicated that the increase of P_n was mainly caused by the increment of Chl a, Chl b and soluble protein. The interactive effects of O₃ and N on the concentrations of P_n, Chl a, Chl b and soluble protein were significant. These results suggest that sufficient N supply can modify the effects of elevated O₃ on photosynthesis and yield in wheat.

Key words: biomass, mediation, nitrogen, ozone, photosynthesis, yield

CHEN Juan, ZENG Qing, ZHU Jian-Guo, LIU Gang, CAO Ji-Ling, XIE Zu-Bin, TANG Hao-Ye, KAZUHIKO Kobayashi. Nitrogen supply mitigates the effects of elevated [O₃] on photosynthesis and yield in wheat[J]. Chin J Plant Ecol, 2011, 35(5): 523-530.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00523

https://www.plant-ecology.com/EN/Y2011/V35/I5/523

Figures/Tables 4

Fig. 1 Interactive effects of elevated O3 and nitrogen on net photosynthesis rate (Pn), stomatic conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr) of the wheat leaves (mean ± SD). ANN, ambient ozone and normal nitrogen. AHN, ambient ozone and high nitrogen; FNN, elevated ozone and normal nitrogen; FHN, elevated ozone and high nitrogen. Different letters mean significance at p < 0.05 level.

Fig. 2 Interactive effects of elevated O3 and nitrogen on the photosynthetic pigment content of wheat leaves (mean ± SD). ANN, ambient ozone and normal nitrogen; AHN, ambient ozone and high nitrogen; FNN, elevated ozone and normal nitrogen; FHN, elevated ozone and high nitrogen. Different letters mean significance at p < 0.05 level.

Fig. 3 Interactive effects of elevated O3 and nitrogen on soluble protein content in wheat leaves (mean ± SD). ANN, ambient ozone and normal nitrogen; AHN, ambient ozone and high nitrogen; FNN, elevated ozone and normal nitrogen; FHN, elevated ozone and high nitrogen. Different letters mean significance at p < 0.05 level.

Table 1 Interactive effects of elevated O3 and nitrogen on the yield and biomass of different growth period (kg·hm-2) of wheat (mean ± SD)

处理 Treatment		总生物量 Total biomass			产量 Yield
处理 Treatment		抽穗期 Heading	开花期 Blooming	成熟期 Maturing	收获期 Harvesting
NN	A	9 003 ± 897	12 195 ± 1235	14 657 ± 140	8 029 ± 396
	F	10 565 ± 1 023	11 700 ± 676	12 865 ± 428	7 014 ± 259
HN	A	9 809 ± 690	12 252 ± 1 310	14 491 ± 1 031	8 224 ± 548
	F	11 324 ± 814	11 831 ± 322	14 787 ± 447	7 762 ± 265
N		ns	ns	ns	ns
O₃		ns	ns	ns	*
N × O₃		ns	ns	ns	ns

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Nitrogen supply mitigates the effects of elevated [O₃] on photosynthesis and yield in wheat

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