Changes of photosynthetic pigment and photosynthetic enzyme activity in stems of Phyllostachys pubescens during rapid growth stage after shooting

doi:10.3724/SP.J.1258.2012.00456

Abstract

Abstract:

Aims Our objective was to reveal photosynthetic characters in Phyllostachys pubescens stems. We determined the distribution of chloroplasts and detected changes of pigment content and photosynthetic enzyme activity in the stems during their rapid growth stage.
Methods We detected the pigment content and photosynthetic enzyme activity by the colorimetric method and observed the distribution of chloroplasts using a laser scanning confocal microscope.
Important findings Chloroplasts were mainly distributed in the ground tissues under the epidermis and in cells around the vascular bundle, similar to Kranz anatomy in C₄ plants. The content of chlorophyll a, chlorophyll b and carotenoid was significantly (p < 0.01) increased with the growth of the stems of P. pubescens. The activities of ribulose-1, 5-bisphosphate carboxylase change/add oxygen enzymes (Rubisco), phosphoenolpyruvate carboxylase (PEPC) and nicotinamide-adenine dinucleotide phosphate-malic enzyme (NADP-ME) in the stems reached the highest level after 10 days, and they declined gradually with the growth of the stems. After 30 days, the activities decreased by 88.55% (p < 0.01), 77.46% (p < 0.01) and 72.50% (p < 0.01), respectively, compared with after 10 days. The ratio of PEPC/Rubisco increased gradually and reached 12.83 after 30 days, which was markedly higher than that in C₃ plants. These results indicated that there was C₄ photosynthetic pathway in the stems, and the pathway may play an important role in the efficient photosynthesis and rapid growth of P. pubescens.

Key words: chloroplast, photosynthetic enzyme, photosynthetic pigment, Phyllostachys pubescens, stem

WANG Xing-Xing, LIU Lin, ZHANG Jie, WANG Yu-Kui, WEN Guo-Sheng, GAO Rong-Fu, GAO Yan, ZHANG Ru-Min. Changes of photosynthetic pigment and photosynthetic enzyme activity in stems of Phyllostachys pubescens during rapid growth stage after shooting[J]. Chin J Plant Ecol, 2012, 36(5): 456-462.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2012.00456

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Figures/Tables 5

Fig. 1 Distribution of chloroplast in the stems of Phyllostachys pubescens. A, Ground tissue under epidermis. B, Ground tissue around the vascular bundle. Chl, chloroplast (red); E, epidermis; F, fiber; Gr, ground tissue; Va, vascular bundle.

Table 1 Changes of pigment contents in the stems of Phyllostachys pubescens under rapid growth stage after shooting (μg·g-1 FW) (mean ± SD)

出笋后的生长天数 Growth days after shooting (d)	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	叶绿素总量 Total chlorophyll	类胡萝卜素Carotenoids	叶绿素a/b Chlorophyll a/b
10	3.9 ± 0.6^eE	3.3 ± 1.2^eE	7.2 ± 1.8^eE	1.0 ± 0.3^eE	1.2 ± 0.8 ^bB
20	5.5 ± 0.8^eE	4.2 ± 1.2^eDE	9.7 ± 2.1^eE	0.9 ± 0.3^eE	1.3 ± 0.4^bB
30	17.7 ± 1.6^dD	7.2 ± 0.7^dD	24.9 ± 0.9^dD	6.5 ± 0.6^dD	2.5 ± 0.5^aA
40	135.3 ± 0.1^cC	65.3 ± 2.2^cC	200.7 ± 2.2^cC	42.1 ± 0.1^cC	2.0 ± 0.2^aA
50	179.8 ± 0.9^bB	94.6 ± 1.7^bB	274.4 ± 1.9^bB	46.2 ± 0.3^bB	2.0 ± 0.3^aA
60	269.6 ± 4.0^aA	128.0 ± 1.2^aA	397.6 ± 5.2^aA	49.8 ± 3.6^aA	2.1 ± 0.1^aA

Fig. 2 Changes of ribulose-1,5-bisphosphate carboxylase change/add oxygen enzymes (Rubisco) activity in the stems of Phyllostachys pubescens (mean ± SD).

Fig. 3 Changes of phosphoenolpyruvate carboxylase (PEPC) and nicotinamide-adenine dinucleotide phosphate-malic enzyme (NADP-ME) activities in the stems of Phyllostachys pubescens (mean ± SD).

Fig. 4 Ratio of phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase change/add oxygen enzymes (PEPC/Rubisco) in the stems of Phyllostachys pubescens (mean ± SD).

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