Chin J Plant Ecol ›› 2012, Vol. 36 ›› Issue (5): 456-462.DOI: 10.3724/SP.J.1258.2012.00456

• Research Articles • Previous Articles     Next Articles

Changes of photosynthetic pigment and photosynthetic enzyme activity in stems of Phyllostachys pubescens during rapid growth stage after shooting

WANG Xing-Xing1, LIU Lin1, ZHANG Jie1, WANG Yu-Kui3, WEN Guo-Sheng1, GAO Rong-Fu2, GAO Yan1, ZHANG Ru-Min1,*()   

  1. 1Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China
    2Beijing Forestry University, Beijing 100091, China
    3China Bamboo Research Center, State Forestry Administration, Hangzhou 310012, China
  • Published:2012-05-04
  • Contact: ZHANG Ru-Min


Aims Our objective was to reveal photosynthetic characters in Phyllostachys pubescens stems. We determined the distribution of chloroplasts and detected changes of pigment content and photosynthetic enzyme activity in the stems during their rapid growth stage.
Methods We detected the pigment content and photosynthetic enzyme activity by the colorimetric method and observed the distribution of chloroplasts using a laser scanning confocal microscope.
Important findings Chloroplasts were mainly distributed in the ground tissues under the epidermis and in cells around the vascular bundle, similar to Kranz anatomy in C4 plants. The content of chlorophyll a, chlorophyll b and carotenoid was significantly (p < 0.01) increased with the growth of the stems of P. pubescens. The activities of ribulose-1, 5-bisphosphate carboxylase change/add oxygen enzymes (Rubisco), phosphoenolpyruvate carboxylase (PEPC) and nicotinamide-adenine dinucleotide phosphate-malic enzyme (NADP-ME) in the stems reached the highest level after 10 days, and they declined gradually with the growth of the stems. After 30 days, the activities decreased by 88.55% (p < 0.01), 77.46% (p < 0.01) and 72.50% (p < 0.01), respectively, compared with after 10 days. The ratio of PEPC/Rubisco increased gradually and reached 12.83 after 30 days, which was markedly higher than that in C3 plants. These results indicated that there was C4 photosynthetic pathway in the stems, and the pathway may play an important role in the efficient photosynthesis and rapid growth of P. pubescens.

Key words: chloroplast, photosynthetic enzyme, photosynthetic pigment, Phyllostachys pubescens, stem