Effects of endophyte fungal species and host plant genotype on the leaf shape and leaf area of endophyte-grass symbionts

doi:10.17521/cjpe.2020.0037

Abstract

Abstract:

Aims Epichloë infection can play an important role in the growth and development of host plants. However, few studies have been reported on the effects of endophyte infection on leaf shape and area of host plants.
Methods Our study used Achnatherum sibiricum as experimental material, and explored the effects of endophyte species and host plant genotype on the leaf shape and area of A. sibiricum-endophyte symbionts through the combination of the length and width method with the revised correction coefficient and the scanning calculation method.
Important findings Endophyte infection status, endophyte species and host plant genotype had no significant influences on the correction coefficient of leaf size calculation, leaf length, leaf width and leaf length-width ratio reflecting leaf shape. By calculation and verification, we determined that the correction coefficient of leaf size calculation of A. sibiricum was 0.594 9. There was no significant difference between the leaf areas measured by length and width method with the revised correction coefficient and the scanning calculation method. In addition, the leaf areas measured by two methods were not significantly affected by endophyte infection, endophyte species and plant maternal genotype.

Key words: fungal endophyte, Achnatherum sibiricum, leaf area, maternal genotype, correction coefficient

QIN Tian-Zi, REN An-Zhi, FAN Xiao-Wen, GAO Yu-Bao. Effects of endophyte fungal species and host plant genotype on the leaf shape and leaf area of endophyte-grass symbionts[J]. Chin J Plant Ecol, 2020, 44(6): 654-660.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2020.0037

https://www.plant-ecology.com/EN/Y2020/V44/I6/654

Figures/Tables 8

Table 1 Analysis of variance for the effects of endophyte infection and host plant genotype on leaf length, width and length-width ratio of Achnatherum sibiricum-endophyte symbionts

	叶长 Leaf length (cm)		叶宽 Leaf width (cm)		长宽比 Length-width ratio
	F	p	F	p	F	p
E	3.503	0.068	0.563	0.457	1.970	0.167
PG (EG)	0.372	0.893	1.955	0.095	1.596	0.171
E × PG (EG)	0.349	0.906	0.801	0.532	1.728	0.143

Table 2 One-way analysis of variance for the effects of endophyte species on leaf length, width and leaf length-width ratio of Achnatherum sibiricum

	叶长 Leaf length (cm)		叶宽 Leaf width (cm)		长宽比 Length-width ratio
	F	p	F	p	F	p
内生真菌种类 Endophyte species	0.190	0.828	1.024	0.374	1.917	0.169

Table 3 Analysis of generalized linear mixed model for the effects of endophyte infection and host plant genotype on leaf length-width product and leaf area of different Achnatherum sibiricum-endophyte symbionts

	羽茅叶面积 Leaf area of A. sibiricum
	F	p
校正模型 Corrected Model	595.131	<0.001
E	1.112	0.292
PG (EG)	0.843	0.537
LWP	15 233.050	<0.001
E × PG (EG)	0.683	0.707
E × LWP	0.707	0.401
PG (EG) × LWP	1.567	0.131
E × PG (EG) × LWP	1.858	0.063

Table 4 The generalized linear mixed model analysis for the effects of endophyte species on leaf length-width product and area of Achnatherum sibiricum

	羽茅叶面积 Leaf area of A. sibiricum
	F	p
校正模型 Corrected Model	2 687.209	<0.001
EG	0.041	0.989
LWP	10 131.957	<0.001
EG × LWP	0.381	0.683

Fig. 1 The linear relationship between the actual leaf area and length-width product of Achnatherum sibiricum. n, total number of leaves.

Fig. 2 The linear relation between actual leaf area and leaf area measured by length-width method. n, selected number of leaves.

Table 5 Analysis of variance for the effects of endophyte infection and host plant genotype on average leaf area of different Achnatherum sibiricum-endophyte symbionts

	实际叶面积 Actual leaf area (cm²)		长宽法测得叶面积 Leaf area measured by length-width method (cm²)
	F	p	F	p
E	1.677	0.202	2.324	0.135
PG (EG)	1.101	0.377	1.256	0.297
E × PG (EG)	0.291	0.937	0.205	0.973

Table 6 One-way analysis of variance for the effects of endophyte species on average leaf area of Achnatherum sibiricum

	实际叶面积 Actual leaf area (cm²)		长宽法测得叶面积 Leaf area measured by length-width method (cm²)
	F	p	F	p
内生真菌种类 Endophyte species	0.843	0.443	0.114	0.892

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