植物生态学报 ›› 2014, Vol. 38 ›› Issue (5): 507-514.DOI: 10.3724/SP.J.1258.2014.00047
收稿日期:
2013-10-09
接受日期:
2014-01-10
出版日期:
2014-10-09
发布日期:
2014-05-13
通讯作者:
董娟娥
基金资助:
XING Bing-Yu1,ZHU Nan1,ZHANG Hong-Pei1,YANG Xi-Ling2,DONG Juan-E1,*()
Received:
2013-10-09
Accepted:
2014-01-10
Online:
2014-10-09
Published:
2014-05-13
Contact:
DONG Juan-E
摘要:
为了探讨甲基紫精(MV)对丹参(Salvia miltiorrhiza)体内抗氧化防护系统的影响及其生理机制。以MV为诱导剂, 以敌草隆(DCMU)为抑制剂, 考察了MV与DCMU处理后丹参悬浮培养细胞中H2O2、丙二醛、还原型谷胱甘肽的含量以及抗氧化防护酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT))活性变化和同工酶的表达差异。结果表明, MV处理显著提高了丹参培养细胞内H2O2、丙二醛以及还原型谷胱甘肽含量; MV处理使CAT、POD活性增强, 谱带颜色更亮, 条带增加。DCMU处理显著抑制了MV诱导的H2O2、丙二醛、还原型谷胱甘肽含量的增加, 抗氧化酶活性的升高和同工酶的表达。以上结果说明, MV可诱导丹参培养细胞叶绿体产生H2O2, H2O2激活了丹参培养细胞抗氧化防护系统以维持细胞正常的生理活动。
行冰玉,朱楠,张洪培,杨喜玲,董娟娥. 甲基紫精对丹参培养细胞抗氧化防护系统的影响. 植物生态学报, 2014, 38(5): 507-514. DOI: 10.3724/SP.J.1258.2014.00047
XING Bing-Yu,ZHU Nan,ZHANG Hong-Pei,YANG Xi-Ling,DONG Juan-E. Effects of methyl viologen on the antioxidant system in cultured Salvia miltiorrhiza cells. Chinese Journal of Plant Ecology, 2014, 38(5): 507-514. DOI: 10.3724/SP.J.1258.2014.00047
图1 不同浓度甲基紫精对丹参培养细胞中H2O2含量的影响(平均值±标准误差)。试验材料为培养6天的丹参悬浮培养细胞。不同小写字母表示差异显著(p < 0.05)。
Fig. 1 Effects of methyl viologen on the accumulation of H2O2 in cultured Salvia miltiorrhiza cells (mean ± SE). Test materials are S. miltiorrhiza cells in suspension cultures for six days. Different small letters indicate significant differences (p < 0.05).
图2 敌草隆(DCMU)和甲基紫精(MV)对丹参培养细胞中H2O2含量的影响(平均值±标准误差)。试验材料为培养6天的丹参悬浮培养细胞。DCMU在MV处理前30 min添加。不同小写字母表示差异显著(p < 0.05)。
Fig. 2 Effects of diuron (DCMU) and methyl viologen (MV) on the accumulation of H2O2 in cultured Salvia miltiorrhiza cells (mean ± SE). Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
图3 不同处理对丹参培养细胞中丙二醛含量的影响(平均值±标准误差)。DCMU, 敌草隆; MV, 甲基紫精。试验材料为培养6天的丹参悬浮培养细胞。DCMU在MV处理前30 min添加。不同小写字母表示差异显著(p < 0.05)。
Fig. 3 Effects of different treatments on the accumulation of malondialdehyde (MDA) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
图4 不同处理对丹参培养细胞中还原型谷胱甘肽含量的影响(平均值±标准误差)。DCMU, 敌草隆; MV, 甲基紫精。试验材料为培养6天的丹参悬浮培养细胞。DCMU在MV处理前30 min添加。不同小写字母表示差异显著(p < 0.05)。
Fig. 4 Effects of different treatments on the accumulation of glutathione (GSH) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
图5 不同处理对丹参培养细胞中超氧化物歧化酶(SOD)(A)、过氧化氢酶(CAT)(B)和过氧化物酶(POD)(C)活性的影响(平均值±标准误差)。DCMU, 敌草隆; MV, 甲基紫精。试验材料为培养6天的丹参悬浮培养细胞。DCMU在MV处理前30 min添加。不同小写字母表示差异显著(p < 0.05)。
Fig. 5 Effects of different treatments on the activities of superoxide dismutase (SOD) (A), catalase (CAT) (B), and peroxidase (POD) (C) in cultured Salvia miltiorrhiza cells (mean ± SE). DCMU, diuron; MV, methyl viologen. Test materials are S. miltiorrhiza cells in suspension cultures for six days. DCMU is added 30 min prior to the MV treatments. Different small letters indicate significant differences (p < 0.05).
图6 不同处理对丹参培养细胞中超氧化物歧化酶(SOD) (A)、过氧化氢酶(CAT)(B)和过氧化物酶(POD)(C)同工酶的影响。1, 蒸馏水处理; 2, 甲基紫精处理; 3, 敌草隆处理; 4, 甲基紫精+敌草隆联合处理。a-e, SOD, CAT, POD的同工酶条带。
Fig. 6 Effects of different treatments on the isoenzymes spectrum of superoxide dismutase (SOD) (A), catalase (CAT) (B), and peroxidase (POD) (C) in culture Salvia miltiorrhiza cells. 1, distilled water treatment; 2, methyl viologen treatment; 3, diuron treatment; 4, methyl viologen + diuron treatment. a-e, soenzyme bands of SOD, CAT and POD.
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