Chin J Plan Ecolo ›› 2018, Vol. 42 ›› Issue (7): 741-751.doi: 10.17521/cjpe.2018.0097

• Research Articles • Previous Articles     Next Articles

Patterns of variations in leaf turgor pressure and responses to environmental factors in Populus tomentosa

LI Dou-Dou1,*, XI Ben-Ye1,*, WANG Fei2, JIA Su-Ping3, ZHAO Hong-Lin4, HE Yue-Lin1, LIU Yang1, JIA Li-Ming1,**()   

  1. 1 Ministry of Education Key Laboratory of Silviculture and Conservation, Beijing Forestry University, Beijing 100083, China
    2 Liaocheng Forestry Bureau, Liaocheng, Shandong 252002, China
    3 Forestry Workstation of Daxing District in Beijing, Beijing 102600, China
    4 Forestry Protection Station of Daxing District in Beijing, Beijing 102600, China
  • Online:2018-06-11 Published:2018-07-20
  • Contact: Dou-Dou LI,Ben-Ye XI,Li-Ming JIA E-mail:jlm@bjfu.edu.cn
  • Supported by:
    Supported by the Key Technologies R&D Program of China(2015BAD09B02);the National Natural Science Foundation of China(31400532);the National Natural Science Foundation of China(31670625);the National Key Research and Development Program of China(2016YFD0600403)

Abstract:

Aims In this study, the patterns of variations in leaf turgor pressure and responses to environmental factors were investigated in Populus tomentosa, in order to provide a theoretical basis for guiding irrigation using leaf turgor pressure as a water stress indicator.

Methods The study was conducted in a two-year-old Populus tomentosaplantation under surface drip irrigation. Leaf patch clamp pressure (Pp) was continuously monitored in treatments of full irrigation (FI) and control (CK). Soil temperature (Ts), soil water potential (Ψs), sap flow velocity (VSF) and meteorological factors were monitored simultaneously. The patterns of variations in leaf turgor pressure and relationships between Pp and environmental factors of P. tomentosa under different water treatments were analyzed.

Important findings The Pp values exhibited a clear pattern of “daytime high and nighttime low” with varying weather conditions, and the width of peak signals in Pp was greatest in sunny days. The positive relationships between normalized leaf patch pressure (ΔPp) and VSF fitted a polynomial function under different weather conditions, with R2 values ranked in the order of sunny days (0.87) > cloudy days (0.72) > rainy days (0.31). Photosynthetically active radiation (PAR), air temperature (Ta), relative air humidity (RH), and vapor pressure deficiency (VPD) were predominant environmental factors affecting Pp, especially PAR. Hysteresis was found between ΔPp and different environmental factors, and the size of the hysteresis loop differed between the two irrigation treatments. There were significant differences in the shapes of Pp curves between the irrigation treatments. In conclusion, the variations of leaf turgor pressure were closely related to environmental factors in P. tomentosa, and there was a high degree of synergistic change with the sap flow on sunny days. Therefore, leaf turgor pressure can potentially serve as a diagnostic indicator of water deficit.

Key words: leaf turgor pressure, ZIM-probe, sap flow velocity, water stress, Populus tomentosa

Table 1

Basic physical and chemical characteristics of the soil at the experimental site"

土壤深度
Soil
depth (cm)
颗粒组成 Particle size distribution (%) 质地1)
Texture1)
容重
Bulk
density (g?cm-3)
田间持水量
Field water-
holding capacity
(cm3·cm-3)
饱和含水量
Saturated
water content
(cm3·cm-3)
有机质
Organic
matter
(g·kg-1)
速效磷
Available
phosphorus (mg·kg-1)
速效钾
Available potassium
(mg·kg-1)
砂粒 Sand 粉粒 Silt 黏粒 Clay
0-50 61.79 35.52 2.70 砂壤土 Sandy loam 1.41 0.34 0.44 4.7 7.26 44.42
50-140 63.92 33.69 2.39 砂壤土 Sandy loam 1.43 0.36 0.45 2.3 0.97 27.85
140-300 29.62 65.54 4.84 粉壤土 Silt loam 1.46 0.35 0.44 2.6 1.63 41.98

Fig. 1

Diagram of ZIM-probe structure and working principle. Pc, the turgor in the leaf patch; Pclamp, the pressure by the magnets on the leaf patch; Pp , the pressure of difference between Pc and Pclamp."

Fig. 2

Variations of soil water potential at 20 cm depth and 0 cm distance from a dripper in treatments of full irrigation (FI) and control (CK)."

Fig. 3

Diurnal changes of leaf patch clamp pressure (Pp) and environmental factors. I-1, 2, 3 and II-1, 2, 3 represent 3 ZIM-probes on leaves of the 1st and 2nd sample trees in the full irrigation (FI) treatment, respectively."

Fig. 4

Diurnal changes in leaf patch clamp pressure (Pp) and sap flow velocity (VSF) under different weather conditions in the full irrigation (FI) treatment."

Fig. 5

Correlation analysis between normalized leaf patch clamp pressure (ΔPp) and environmental factors. Hourly average ΔPp from June 1st to 12th in the full irrigation (FI) treatment."

Table 2

Correlation analysis between daily normalized leaf patch clamp pressure (ΔPp) and environmental factors"

环境因子
Environmental factors
光合有效辐射
Photosynthetically
active radiation
空气温度
Air temperature
风速
Wind speed
空气相对湿度
Relative
air humidity
饱和水气压差
Vapor pressure
deficiency
土壤温度
Soil
temperature
相关系数 Correlation coefficient -0.080 0.066 0.313 -0.332 0.236 0.287
显著性水平 Sig. 0.804 0.838 0.321 0.291 0.461 0.365

Fig. 6

Variations in leaf patch clamp pressure (Pp) curves under different water treatments. The red arrow shows irrigation in the full irrigation (FI) treatment; nocturnal hours (18:00-6:00) are marked by grey columns, and the period of June 10th-12th is marked by red rectangle when significant difference of Pp peaks occurs between the full irrigation (FI) and control (CK) treatments."

Fig. 7

Hysteresis between normalized leaf patch clamp pressure (ΔPp) and environmental factors. Hourly average ΔPp from June 1st to 12th in full irrigation (FI) and control (CK) treatments, respectively."

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