EFFECTS OF WATER STRESS ON OSMOLYTES AT DIFFERENT GROWTH STAGES IN RICE LEAVES AND ROOTS

doi:10.3773/j.issn.1005-264x.2008.02.029

Abstract

Abstract:

Aims Water stress is one of the most important ecological factors affecting yield and quality of rice (Oryza sativa), and osmotic adjustment is the main mechanism for the crop to adjust to drought. Our objective was to elucidate the effects of water stress on osmolytes at different growth stages in leaves and roots of rice.
Methods We used the rice variety Feng-Hua-Zhan grown in pots to study the effects of water stress on inorganic and organic osmolytes in leaves and roots. Water was withheld 15 d at the growth stages of tillering, panicle differentiation, heading and filling.
Important findings Water stress significantly decreased leaf water potential. Organic osmolytes including soluble sugar, proline and free amino acid and inorganic osmolytes including K⁺ and Mg²⁺ in leaves and roots increased significantly after drought treatment at different growth stages. These osmolytes could be reduced to normal levels after re-watering at the tillering stage, but not at the panicle differentiation and heading stages. Osmolytes accumulated to higher levels after drought treatment at panicle differentiation and heading stages than at other growth stages, and organic osmolytes accumulated to higher levels than inorganic osmolytes in the different treatments. The order of osmotic adjustment (OA) ability at different growth stages was: heading, panicle differentiation, filling and tillering. The OA in roots was lower than but positively correlated to OA in leaves. Roots were more sensitive and had a more rapid response to water than did leaves. The order of contribution for osmotic adjustment in leaves and roots with different osmolytes was: K⁺ > Ca ²⁺ > soluble sugar > Mg ²⁺ > free amino acid > proline.

Key words: rice, water stress, osmotic adjustment, osmolyte, root system, leaf

CAI Kun-Zheng, WU Xue-Zhu, LUO Shi-Ming. EFFECTS OF WATER STRESS ON OSMOLYTES AT DIFFERENT GROWTH STAGES IN RICE LEAVES AND ROOTS[J]. Chin J Plant Ecol, 2008, 32(2): 491-500.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2008.02.029

https://www.plant-ecology.com/EN/Y2008/V32/I2/491

Figures/Tables 7

Table 1 Change of leaf water potential after water stress at different growth stages (-MPa) (Mean±SD)

处理 Treatment	移栽后天数 Days after transplanting (d)
处理 Treatment	15	30	50	80	95
CK	0.91±0.03^a	0.72±0.05^a	0.55±0.02^a	1.08±0.10^a	1.25±0.06^a
T₁		1.01±0.11^b	0.59±0.04^a	1.12±0.03^a	1.22±0.09^a
T₂			1.51±0.06^b	1.19±0.11^a	1.13±0.05^a
T₃				2.29±0.17^b	1.11±0.10^a
T₄					2.07±0.15^b

Fig.1 Change of soluable sugar content in leaf and root of rice after water stress at different stages

Fig.2 Change of proline content in leaf and root of rice after water stress at different stages

Fig.3 Change of free amino acid content in leaf and root of rice after water stress at different stages

Fig.4 Change of K+ content in leaf and root of rice after water stress at different stages

Fig.5 Change of Ca2+ content in leaf and root of rice after water stress at different stages

Fig.6 Change of Mg2+ content in leaf and root of rice after water stress at different stages

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