不同光强下细胞外三磷酸腺苷对菜豆叶片光化学反应特性的影响

doi:10.3724/SP.J.1258.2014.00106

摘要/Abstract

摘要：

三磷酸腺苷(ATP)不但分布在细胞内部, 而且广泛存在于动物和植物细胞的细胞外基质中。细胞外ATP (eATP)可与细胞膜表面相应的受体结合并激发细胞内的第二信使, 从而调节细胞的多种生理学功能。但目前对于eATP是否也能对植物的光合作用产生影响则研究较少。该文以菜豆(Phaseolus vulgaris)叶片为实验材料, 研究了在不同光强下eATP对菜豆叶片叶绿素荧光特性和光合放氧速率的影响。结果显示, 随着光强的增加, 叶片的光适应下最大光化学效率(F_v′/F_m′)、光系统II (PSII)实际光化学效率(Y(II))、光化学猝灭系数(q_P)均呈现下降趋势, 而电子传递速率(ETR)、非光化学猝灭系数(q_N)以及调节性能量耗散的量子产量(Y(NPQ))随着光强的增加呈上升趋势。与对照相比, eATP的处理可以显著提高菜豆叶片PSII的潜在最大光化学效率(F_v/F_m)、Y(II)、q_P、ETR和光合放氧速率; 但eATP的处理对F_v′/F_m′、q_N以及Y(NPQ)没有显著影响。AMP-PCP (β,γ-亚甲基三磷酸腺苷, eATP细胞外受体的抑制剂)的处理显著降低了F_v/F_m、F_v′/F_m′、Y(II)、ETR和光合放氧速率, 同时也显著增加了q_N以及Y(NPQ)的水平。以上结果显示, 植物eATP水平的变化对植物光合作用的光化学反应有着重要的影响。

关键词: 菜豆, 细胞外ATP, 光强, 叶绿素荧光参数

Abstract:

Aims Although adenosine 5′-triphosphate (ATP) is usually considered to be localized in intracellular spaces, plant and animal cells can secrete ATP from the cytosol into the extracellular matrix. This extracellular ATP (eATP) is an important signal molecule for many physiological responses in plants. However, whether eATP could also have effects on photosynthesis in plants has not been extensively studied.
Methods With bean (Phaseolus vulgaris) leaves as experiment material, the effects of eATP on the chlorophyll fluorescence characteristics and photosynthetic O₂evolution rate were studied under different light intensities.
Important findings The maximal photosystem II (PSII) quantum yield in light adaptation (F_v′/F_m′), effective photochemical quantum yield of PSII (Y(II)), and photochemical quenching coefficient (q_P) gradually decreased, and the electron transport rate (ETR), non-photochemical quenching coefficient (q_N), and the quantum yield of regulated energy dissipation (Y(NPQ)) increased, with increases in light intensity. Treatment of leaves with eATP significantly increased the values of the potential maximal photochemical efficiency of PSII (F_v/F_m), F_v′/F_m′,Y(II), q_P, ETR, and photosynthetic O₂evolution rate, but did not affect the values of q_N and Y(NPQ). In contrast, treatment of leaves with β,γ-methyleneadenosine triphosphate (AMP-PCP, an inhibitor of eATP receptors) significantly reduced the values of F_v/F_m, F_v′/F_m′,Y(II), ETR, and photosynthetic O₂evolution rate, but increased the values of q_N and Y(NPQ). These results show that the levels of eATP exert important influences on the photochemical reaction in photosynthesis.

Key words: bean, chlorophyll fluorescence parameters, extracellular ATP, light intensity

冯汉青, 焦青松, 田武英, 孙坤, 贾凌云. 不同光强下细胞外三磷酸腺苷对菜豆叶片光化学反应特性的影响. 植物生态学报, 2014, 38(10): 1117-1123. DOI: 10.3724/SP.J.1258.2014.00106

FENG Han-Qing, JIAO Qing-Song, TIAN Wu-Ying, SUN Kun, JIA Ling-Yun. Effects of extracellular ATP on the characteristics of photochemical reaction in bean (Phaseolus vulgaris) leaves under different light intensities. Chinese Journal of Plant Ecology, 2014, 38(10): 1117-1123. DOI: 10.3724/SP.J.1258.2014.00106

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2014.00106

https://www.plant-ecology.com/CN/Y2014/V38/I10/1117

图/表 4

图1 细胞外ATP及AMP-PCP对菜豆叶片PSII潜在最大光化学效率(Fv/Fm)的影响(平均值±标准偏差, n = 4)。 *, p < 0.05。

Fig. 1 Effects of extracellular ATP and AMP-PCP on potential maximal photochemical efficiency (Fv/Fm) in bean leaves (mean ± SD, n = 4). *, p < 0.05.

图2 细胞外ATP及AMP-PCP对菜豆叶片光照下PSII光化学效率和电子传递的影响(平均值±标准偏差, n = 4)。 *, p < 0.05。

Fig. 2 Effects of extracellular ATP and AMP-PCP on photochemical efficiency and electron transport rate in bean leaves under light (mean ± SD, n = 4). *, p < 0.05. ETR, electron transport rate; Fv'/Fm', maximal photosystem II quantum yield in light adaptation; qp, photochemical quenching coefficient; Y(II), effective photochemical quenching yield.

图3 细胞外ATP及AMP-PCP对菜豆叶片光照下光合放氧速率的影响(平均值±标准偏差, n = 4)。 *, p < 0.05。

Fig. 3 Effects of extracellular ATP and AMP-PCP on photosynthetic O2 evolution rate in bean leaves under light (mean ± SD, n = 4). *, p < 0.05.

图4 ATP及AMP-PCP对菜豆叶片光照下能量耗散的影响(平均值±标准偏差, n = 4)。 *, p < 0.05。

Fig. 4 Effects of ATP and AMP-PCP on energy dissipation in bean leaves under light (mean ± SD, n = 4). *, p < 0.05. qN, non-photochemical quenching coefficient; Y(NPQ), quantum yield of regulated energy dissipation.

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