两种杂交杨叶绿素荧光特性及光能利用

doi:10.3773/j.issn.1005-264x.2009.06.015

摘要/Abstract

摘要：

比较研究了伊犁地区两种杂交杨伊犁杨1号(Populus deltaids‘cv-64’ (P64))和伊犁杨小叶杨(P. simonii canaden×P. russkii-9(Jia))对太阳辐射光能的利用和耗散特性。两种杂交杨光合速率(P_n)的日变化均呈现双峰型, 高光量子通量密度(PFD)阶段P_n达到20.1%的差距; 实际最大光化学猝灭ΦPSII日变化均呈“U”型, 于16:30时, P64的ΦPSII达到最低值, 而Jia的值于14:30时达到最低(Jia>P64); 光合系统的闭合程度(L)在14:30时均出现一个短暂回落, 全天平均闭合程度无显著差异(p>0.05)。非光化学猝灭系数(NPQ)在16:30同时达到最大值(Jia>P64), 两者NPQ全天相差31.7%。叶片转化吸收太阳能热能耗散(E.D)和光化学反应转化的光能量(E.P)进行估算表明: 在PFD较低的环境条件下, 两种杂交杨将吸收的光能50%以上用于光化学猝灭; 在PFD较高的环境条件下, P64的E.P值比例大于Jia, 两者全天的E.P值没有太大的差异, P64的E.D值显著大于Jia的E.D值(p>0.01), 而P64的E.D值占全天转化能量的比例小于Jia。P64和Jia的E.P达到最大的估算值时, 其E.D也达到了最大。分析结果表明: 两种杂交杨对高光能形成不同的适应机制, P64利用更多的太阳能进行光化学猝灭反应, 而Jia利用更多的太阳能进行非光化学猝灭反应, 减缓强太阳辐射伤害; 两种杂交杨用于光化学能量分配的比例P64大于Jia, 而P_n值在强辐射阶段和全天平均值、累积值均出现Jia>P64。结果证明, 仅通过杂交杨本身叶绿体对光的荧光特性反应计算接收到的光能中有多少能量被利用与实际植物光合速率的转化的干物质存在一定的差异, 两种杂交杨光化学实际固定碳和转化光能的多少的内在关系需进一步的研究。

关键词: 光化学猝灭, 非光化学猝灭, 光能转化, 热能耗散, 光量子通量密度

Abstract:

Aims Our objective was to study two widely planted hybrid poplars, Populus deltaids‘cv-64’ (P64) andP. simonii canaden × P. russkii-9 (Jia), to explore their growth mechanisms and the solar energy utilization by photosystem II (PSII) antennae to thermal energy dissipation and photosynthetic electron transport characteristics during long-term adaptation to the ambient environment.
Methods We studied energy utilization by computing chlorophyll fluorescence parameters and photosynthesis. Plants were grown at the Plain Forest Farm Nursery of the State Forestry Bureau of Ili Autonomous Prefecture in China’s Xinjiang region.
Important findings The diurnal changes of net photosynthesis (P_n) were bimodal and had a 20.1% gap under high photon flux density (PFD). The actual efficiency of open PSII centers (ΦPSII) were all of the “U” type, and their average values were equal. The ΦPSII of P64 and Jia reached the minimum value at 16:30 and 14:30, respectively. The closure value had one wave hollow at 14:30, and there was no significant difference (p>0.05) in whole-day average values. The non-photochemical quenching coefficients (NPQ) all achieved the maximum value at 16:30. Jia’sNPQ maximum was less than that of P64, and the whole-day NPQ difference reached 31.7%. The estimated rates of photochemistry (E.P) and non-photochemistry (E.D) showed that under the lower PFD the poplars used more than 50% of the absorbed solar energy for the photochemical quenching, and under high PFD the absorbed solar energy for P64 for the photochemical quenching is greater than the proportion for Jia, while the absorbed solar energy for P64 for heat dissipation is less than the proportion for Jia. The E.Phad no difference and reached the maximum estimated value when itsE.Dreached the maximum. P64 tended to use more absorbed solar energy for photochemical quenching, but Jia tended to use more solar energy for non-photochemical quenching. The cumulative values and average values of the P_n for Jia were much larger than values for P64, but the absorbed solar energy of P64 is greater than the proportion of Jia for the photochemical quenching. These results prove that it is inadequate to calculate the absorbed energy only by the chloroplast fluorescent characteristics of light received.

Key words: photochemical quenching, non-photochemical quenching, energy utilization, energy dissipation

尤鑫, 龚吉蕊, 葛之葳, 段庆伟, 安然, 陈冬花, 张新时. 两种杂交杨叶绿素荧光特性及光能利用. 植物生态学报, 2009, 33(6): 1148-1155. DOI: 10.3773/j.issn.1005-264x.2009.06.015

YOU Xin, GONG Ji-Rui, GE Zhi-Wei, DUAN Qing-Wei, AN Ran, CHEN Dong-Hua, ZHANG Xin-Shi. LIGHT ENERGY UTILIZATION AND CHLOROPHYLL FLUORESCENCE IN TWO CROSSBREED POPLARS. Chinese Journal of Plant Ecology, 2009, 33(6): 1148-1155. DOI: 10.3773/j.issn.1005-264x.2009.06.015

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.06.015

https://www.plant-ecology.com/CN/Y2009/V33/I6/1148

图/表 4

图1 光合系统II和净光合速率的日变化 P64: 伊犁杨1号 Populus deltaids ‘cv-64’ JIA: 伊犁杨小叶杨 P. simonii canaden×P. russkii-9 PAR: 光合有效辐射 Photosynthetically active radiatin NPQ, qN: 非光化学猝灭 Non-photochemical quenching

Fig.1 Diurnal changes of the photosystem II and the net photosynthesis

图2 光合系统、荧光参数能量分配估算值日变化特征 P: PSII天线色素吸收的能量中用于光化学反应的相对份额Fractions of absorbed light utilized in photodynthesis D: 天线色素热耗散的相对份额Fractions of absorbed light utilized in heat dissipation E: 过剩激发能Excitated engery E.P: PSII天线色素吸收的能量中用于光化学反应的能量相对估算值The estimated rates of photochemistry E.D: 线色素热耗散的能量相对估算值The estimated rates of non-photochemistry P64, Jia: 同图1 See Fig.1

Fig. 2 Diurnal changes of the closure of photosystem II, energy parameters and estimated rates of photochemistry

图3 根据P, D和E计算的不同时段的能量分配比例图注同图2 Notes see Fig. 2

Fig. 3 Energy proration during the different period of time based on P, D and E

图4 非光化学淬灭NPQ和PFD拟合特征及光下可变荧光参数Fv′/Fm′与Fo′的拟合预测 Fv′/Fm′: 实际PSⅡ光化学猝灭效率 Photochemical efficiency of PSⅡ in the light Fo′: 光下荧光的初始值 The initial fluorescence in light P64, Jia, NPQ: 同图1 See Fig.1

Fig. 4 Characteristics of NPQ, qn and Photon flux density fitting

参考文献 21

[1]	Bilger W, Bjorkman O (1990). Role of the xanthophylls cycle in photoprotection elucidated by measurements of light induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynthesis Research, 25,173-185. DOI URL PMID
[2]	Crofts AR, Baroli I, Kramer D, Taoka S (1993). Kinetics of electron transfer between Q _A and Q _B in wild type and herbicide-resistant mutants of Chlamydomonas reinhardtii. Z Naturforsch, 48,259-266.
[3]	Chen YZ (陈贻竹), Li XP (李晓萍), Xia L (夏丽), Guo JY (郭俊彦) (1995). The application of chlorophyll fluorescence technique in the study of responses of plants to environmental stresses. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 3(4):79-86. (in Chinese)
[4]	Demmig-Adams B, Adams WW Ⅲ, Barker DH, Logan BA, Bowling DR, Verhoeven AS (1996). Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. Physiologia Plantarum, 98,253-264.
[5]	Demmig-Adams B, Adams WW Ⅲ (1992). Photoprotection and other responses of plants to high light stress. Annual Review of Plant Physiology and Plant Molecular Biology, 43,599-626.
[6]	Demmig-Adams B, Adams WW Ⅲ (1993). The xanthophylls cycle. In: Young AJ, Britton G eds. Carotenoids in Photosynthesis. Chapman & Hall, London, 206-251.
[7]	Demmig-Adams B, Adams WW Ⅲ (1996). Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among higher plant species. Planta, 198,460-470.
[8]	Forcel L, Critchley C, van Rensen JJS (2003). New fluorescence parameters for monitoring photosynthesis in plants. Photosynthesis Research, 78,17-33. DOI URL PMID
[9]	Genty B, Briantais JM, Baker NR (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochemica et Biophysica Acta, 990,87-92.
[10]	Harbinson J, Genty B, Barker NR (1989). Relationship between the quantum efficiencies for photosystemⅠand II in pea leaves. Plant Physiology, 90,1029-1034. DOI URL PMID
[11]	Krause GH, Virgo A, Winter K (1997). High susceptibility to photoinhibition of young leaves of tropical forest trees. Planta, 583-591. DOI URL PMID
[12]	Krause GH, Weis E (1991). Chlorophyll fluorescence and photosynthesis. The basics. Annual Review of Plant Physiology and Plant Molecular Biology, 42,313-349. DOI URL
[13]	Krause GH, Weis E (1984). Chlorophyll fluorescence as a tool in plant physiology PSII interpretation of fluorescence signals. Photosynthesis Research, 5,139-157. DOI URL PMID
[14]	Osmond CB (1994). What is photoinhibition? Some insights from comparisons of shade and sun plants.In: Baker RR, Bowyer JR eds. Photoinhibition of Photosynthesis: from Molecular Mechanisms to the Field. BIOS Scientific Publishers, Oxford, 1-24.
[15]	Quick WP, Stitt M (1989). An examination of factors contributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves. Biochimica et Biophysica Acta, 977,287-296.
[16]	Schubert H, Andersson M, Snoeijs P (2006). Relationship between photosynthesis and non-photochemical quenching of chlorophyll fluorescence in two red algae with different carotenoid compositions. Marine Biology, 149,1003-1013.
[17]	Shi SB (师生波), Li HP (李和平), Wang XY (王学英), Li HM (李惠梅), Han F (韩发) (2007). Utilization and dissipation of strong solar radiation in two alpine plants. Anisodus tanguticus and Rheum tanguticum. Journal of Plant Ecology(Chinese Version)(植物生态学报), 31,129-137. (in Chinese with English abstract)
[18]	Shirke PA, Pathre UV (2003). Diurnal and seasonal changes in photosynthesis and photosystem 2 photochemical efficiency in Prosopis juliflora leaves subjected to natural environmental stress. Photosynthetica, 41,83-89.
[19]	Synkova H, Wilhelmova N, Haisel D (1997). Comparison of chlorophyll fluorescence kinetics and photochemical activities of isolated chloroplasts in genetic analysis of Lycopersicon esculentum Mill hybrids. Photosynthetica, 34,427-438.
[20]	Yang XH (杨兴洪), Zou Q (邹琦), Zhao SJ (赵世杰) (2005). Photosynthetic characteristics and chlorophyll fluorescence in leaves of cotton plants grown in full light and 40% sun light. Acta Phytoecologica Sinica(植物生态学报), 29,8-15. (in Chinese with English abstract)
[21]	Zheng SX (郑淑霞), Shangguan ZP (上官周平) (2006). Comparison of leaf gas exchange and chlorophyll fluorescence parameters in eight broad-leaved tree species. Acta Ecologia Sinica(生态学报), 26,1080-1087. (in Chinese with English abstract)