CO2 DIFFUSIONAL AND STOMATAL LIMITATIONS OF PHOTOSYNTHESIS IN AMOMUM VILLOSUM

doi:10.17521/cjpe.2005.0078

Abstract

Abstract:

Photosynthetic rate is a function of not only the CO₂ concentration gradient between the outside and inside of the leaf, but also the CO₂ diffusional resistance. It is accepted that stomatal resistance is the greatest factor that controls CO₂ diffusional resistance and is thus a crucial factor influencing photosynthesis. Hence, adequate analysis of CO₂ diffusional resistance is necessary for understanding photosynthesis. Intercellular CO₂ concentrations (C_i) are often utilized to calculate stomatal limitation (L_s). This traditional analysis is unreliable when plants are under stress, because C_i cannot be accurately measured under such conditions. Here we introduced the concept of diffusional limitation and introduced a new method to calculate the diffusional limitation value (L_d) without using C_i. In addition, C_i, estimated indirectly through chlorophyll fluorescence parameters (C_i'), was used to calculate a new stomatal limitation value (L_s') for plants grown under 40% relative soil moisture (RSM). We compared the L_s', L_s and L_d for Amomum villosum grown under both 100% and 40% RSM. Photosynthetic rates (P_n) and stomatal conductance (G_s) decreased after noon in both RSM treatments, and P_n and G_s were both higher in 100% RSM than in 40% RSM. Under 100% RSM, L_s did not increase after noontime in A. villosum, indicating stomatal limitation of photosynthesis did not increase, whereas L_d increased indicating the diffusional limitation of photosynthesis increased due to the relatively high mesophyll resistance. Under 40% RSM, L_s' increased sooner than L_d after noon, indicating stomatal resistance was the dominant factor controlling diffusional resistance. In contrast, L_s, calculated using the traditional stomatal limitation method, did not increase under 40% RSM, which might lead us to the wrong conclusion that stomatal limitation was not a factor. The estimated value, C_i', was lower than C_i, indicating that gas exchange system was overestimated using C_i under conditions of water stress. Our results suggest that the traditional method is unreliable under soil water stress conditions, and the two new methods presented are more reliable. Furthermore, mesophyll resistance can be estimated indirectly through the joint analysis of diffusional and stomatal limitation.

Key words: Photosynthetic rate, Intercellular CO₂ concentration, Diffusional limitation, Stomatal limitation, Amomum villosum

LI Xin, FENG Yu-Long. CO₂ DIFFUSIONAL AND STOMATAL LIMITATIONS OF PHOTOSYNTHESIS IN AMOMUM VILLOSUM[J]. Chin J Plant Ecol, 2005, 29(4): 584-590.

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https://www.plant-ecology.com/EN/Y2005/V29/I4/584

Figures/Tables 6

Fig.1 The regressive relationship between leaf temperature and dark respiration rate (Rd) for Amomum villosum

Fig.2 The regressive relationship between leaf temperature and Rubisco specificity factor (S) for Amomum villosum

Fig.3 The regressive relationship between the ratio of non-cycling quantum yield of PSⅡ electron transport to net photosynthetic rate (ΦPSⅡ/Pn) and intercellular CO2 concentration (Ci) for Amomum villosum grown under 100% relative Siol moisture

Fig.4 The diurnal changes of net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and non-cycling quantum yield of PSⅡ electron transport (ΦPSⅡ) for Amomum villosum grown under different relative soil moistures The data were the mean ± SE of 4 independent experiments. Different letters indicated significant difference within treatment. * indicated significant difference between treatments(p < 0.05)

Fig.5 The diurnal changes of diffusional limitation (Ld) and stomatal limitation (Ls) for Amomum villosum grown under different relative soil water moistures Notes see Fig.4

Fig.6 Intercellular CO2 concentration calculated by gas exchange system (Ci) and estimated through chlorophyll fluorescence parameters (Ci') for Amomum villosum grown under 40% RSM Notes see Fig.4

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CO₂ DIFFUSIONAL AND STOMATAL LIMITATIONS OF PHOTOSYNTHESIS IN AMOMUM VILLOSUM

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