Chin J Plan Ecolo ›› 2006, Vol. 30 ›› Issue (3): 465-471.DOI: 10.17521/cjpe.2006.0062

• Research Articles • Previous Articles     Next Articles

THE PROTECTIVE EFFECTS OF ETHYLENE PRODUCTION INHIBITORS  ON VICIA FABASEEDLING LEAVES UNDER HEAT STRESS

JIAO Jian1; LI Chao_Zhou2,3; HUANG Gao_Bao4*   

  1. 1 College of Forestry, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 4 College of Agriculture, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2006-05-30 Published:2006-05-30
  • Contact: HUANG Gao_Bao

Abstract:

Here, we studied the effects of ethylene production inhibitors (cobalt chloride  (CoCl2) and aminoethoxyvinylglycine (AVG)) on the leaves of Vicia faba seedlings under heat stress. Ethylene production in the leaves was inhibited significa ntly before heat stress and after 12 h heat stress when the seedlings were irrigated with 20 μmol·L-1 CoCl2 or 20 μmol·L-1 AVG solution for 7 days. Treatment with 20 μmol·L-1 CoCl2 increased significantly the activities of  SOD, CAT and  APX before heat stress, while the treatment with 20 μmol·L-1 AVG had no significant influences. Treatments with 20 μmol·L-1 CoCl2  and 20 μmol·L-1 AVG did not influence the contents of putrescine (Put), spermidine (Spd), spermine (Spm), chlorophyll, and the production rate of reactive oxygen species (ROS). However, after 12 h heat stress of 45 ℃, treatments significantly promoted the above values. These results  showed that when plants suffered 12 h heat stress of 45 ℃, treatment with 20 μmol·L-1 CoCl2  and 20 μmol·L-1 AVG inhibited ethylene production, increased the content of these  amines and enhanced the activities of the anti_oxidative enzymes, restrained the enhancement in the content of ROS, and alleviated the reduction in membrane stability index, by which the leaves of Vicia faba seedlings were protected.