EFFECTS OF REACTIVE OXYGEN SPECIES ON UV-B-INDUCED ETHYLENE PRODUCTION IN LEAVES OF MAIZE SEEDLINGS

doi:10.17521/cjpe.2007.0120

Abstract

Abstract:

Aims Ethylene accumulation and oxidative stress are two common responses of plants to environmental stresses; however, little is known about their relationships. Our objective was to investigate the role of reactive oxygen species (ROS) in ethylene synthesis induced by UV-B radiation (280-320 nm) in leaves of maize (Zea mays).

Methods Lamps were suspended above and perpendicular to the plastic trays and filtered with 0.13-mm thick cellulose diacetate (transmission down to 290 nm) for UV-B irradiance. The desired UV-B irradiation was obtained by changing the distance between the lamps and the plastic trays. The levels of UV-B irradiation were 4.8 kJ·m^-2·d^-1.

Important findings UV-B radiation led to the generation of ROS and ethylene. The accumulation of ethylene induced by UV-B was not only inhibited by the scavengers of ROS, but also by aminoxyacetic acid (AOA) and 2-aminoethoxyvinlglycine (AVG), which are specific inhibitors of ethylene synthesis. The inhibition effect of ROS scavengers on UV-B-induced ethylene production was reversed by $\text{O}_{2}^{{\bar{.}}}$ donors. Results indicated that the increase in ethylene production may not be the cause of the increase in ROS production under UV-B stress. In contrast, the increase of ROS led to the accumulation of ethylene. Results suggested that ROS are involved in UV-B stress-induced ethylene accumulation. The accumulation of UV-B-induced ethylene was not affected by DPI, an inhibitor of NADPH oxidase and CAT, a specific scavenger of H₂O₂. Results suggested that the effect of H₂O₂ on UV-B-induced ethylene production in leaves of maize seedlings can be excluded, $\text{O}_{2}^{{\bar{.}}}$ plays an important role in UV-B-induced ethylene synthesis in leaves of maize seedlings and $\text{O}_{2}^{{\bar{.}}}$ serves as a potential mediator of ethylene production that the plant can sense the UV-B stress. The related ROS are not from plasma membrane NADPH oxidase. The source of ROS contributing to ethylene accumulation under UV-B stress is unknown.

Key words: reactive oxygen species (ROS), ultraviolet-B radiation (UV-B), ethylene production, maize

WANG Yi-Bo, FENG Hu-Yuan, QU Ying, CHENG Jia-Qiang, $\boxed{\hbox{WANG Xun-Ling}}$ , AN Li-Zhe. EFFECTS OF REACTIVE OXYGEN SPECIES ON UV-B-INDUCED ETHYLENE PRODUCTION IN LEAVES OF MAIZE SEEDLINGS[J]. Chin J Plant Ecol, 2007, 31(5): 946-951.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2007.0120

https://www.plant-ecology.com/EN/Y2007/V31/I5/946

Figures/Tables 4

Fig.1 Effects of UV-B radiation on the rate of $\text{O}_{2}^{{\bar{.}}}$ (a) 、H2O2(b) and ethylene (c)production in leaves of maize seedlings Bars represent standard deviations of three independent experiments with three replicates; Values are means of three triplicate±SE

Fig.2 Effects of AOA and AVG on UV-B induced $\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b) and ethylene(c) accumulation in leaves of maize seedlings Note see Fig. 1

Fig.3 Effects of exogenous ROS scavengers on UV-induced $\text{O}_{2}^{{\bar{.}}}$ (a)、H2O2(b) and ethylene(c) accumulation in leaves of maize seedlings Note see Fig. 1

Fig.4 Effects of exogenous $\text{O}_{2}^{{\bar{.}}}$ donor on UV-induced ethylene accumulation in leaves of maize seedlings after treatments for 5 days Note see Fig. 1

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