THE PROTECTIVE EFFECTS OF ETHYLENE PRODUCTION INHIBITORS ON VICIA FABA SEEDLING LEAVES UNDER HEAT STRESS

doi:10.17521/cjpe.2006.0062

Abstract

Abstract:

Here, we studied the effects of ethylene production inhibitors (cobalt chloride (CoCl₂) and aminoethoxyvinylglycine (AVG)) on the leaves of Vicia faba seedlings under heat stress. Ethylene production in the leaves was inhibited significantly before heat stress and after 12 h heat stress when the seedlings were irrigated with 20 μmol·L ^-1 CoCl₂ or 20 μmol·L ^-1 AVG solution for 7 days. Treatment with 20 μmol·L ^-1 CoCl₂ increased significantly the activities of SOD, CAT and APX before heat stress, while the treatment with 20 μmol·L ^-1 AVG had no significant influences. Treatments with 20 μmol·L ^-1 CoCl₂ and 20 μmol·L ^-1 AVG did not influence the contents of putrescine (Put), spermidine (Spd), spermine (Spm), chlorophyll, and the production rate of reactive oxygen species (ROS). However, after 12 h heat stress of 45 ℃, treatments significantly promoted the above values. These results showed that when plants suffered 12 h heat stress of 45 ℃, treatment with 20 μmol·L ^-1 CoCl₂ and 20 μmol·L ^-1 AVG inhibited ethylene production, increased the content of these amines and enhanced the activities of the anti-oxidative enzymes, restrained the enhancement in the content of ROS, and alleviated the reduction in membrane stability index, by which the leaves of Vicia faba seedlings were protected.

Key words: Aminoethoxyvinylglycine, Cobalt, Ethylene, Heat stress, Vicia faba

JIAO Jian, LI Chao-Zhou, HUANG Gao-Bao. THE PROTECTIVE EFFECTS OF ETHYLENE PRODUCTION INHIBITORS ON VICIA FABA SEEDLING LEAVES UNDER HEAT STRESS[J]. Chin J Plant Ecol, 2006, 30(3): 465-471.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2006.0062

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Figures/Tables 5

Fig.1 Influences of different concentrations of CoCl2 and aminoethoxyvinyl- glycine (AVG) on the membrane stability index (MSI) of Vicia faba seedlings leaves after 0 and 12 h heat stress of 45 ℃ Different capital letters above the bars indicate significant differences (p<0.05) of different treatments before or after heat stress, different small letters indicate significant differences (p<0.05) of the same treatment before and after heat stress

Fig.2 Influences of 20 μmol·L -1 CoCl2 and 20 μmol·L -1 aminoethoxyvin-ylglycine (AVG) on ethylene production and content of putrescine (Put), spermidine (Spd) and spermine (Spm) in the leaves of Vicia faba seedlings after 0 and 12 h heat stress of 45 ℃ Different capital letters above the bars indicate significant differences (p<0.05) of different treatments before or after heat stress, different small letters indicate significant differences (p<0.05) of the same treatments before and after heat stress

Fig.3 Influences of 20 μmol·L -1 CoCl2 and 20 μmol·L -1 aminoethoxyvin- ylglycine (AVG) on the content of thiobarbituric acid reacting substances (TBARS) and chlorophyll in the leaves of Vicia faba seedlings after 0 and 12 h heat stress of 45 ℃ Different capital letters above the bars indicate significant differences (p<0.05) of different treatments before or after heat stress, different small letters indicate significant differences (p<0.05) of the same treatments before and after heat stress

Fig.4 Influences of 20 μmol·L -1 CoCl2 and 20 μmol·L -1 aminoethoxyvin- ylglycine (AVG) on the production rate of H2O2 and O 2 - · in the leaves of Vicia faba seedlings after 0 and 12 h heat stress of 45 ℃ Different capital letters above the bars indicate significant differences (p<0.05) of different treatments before or after heat stress, different small letters indicate significant differences (p<0.05) of the same treatments before and after heat stress

Fig.5 Influences of 20 μmol·L -1 CoCl2 and 20 μmol·L -1 aminoethoxyvin- ylglycine (AVG) on the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in the leaves of Vicia faba seedlings after 0 and 12 h heat stress of 45 ℃ Different capital letters above the bars indicate significant differences (p<0.05) of different treatments before or after heat stress, different small letters indicate significant differences (p<0.05) of the same treatments before and after heat stress

References 36

[1]	Abraham JL, Hunt A (1995). Environmental contamination by cobalt in the vicinity of a cemented tungsten carbide tool grinding plant. Environmental Research, 69,67-74. DOI URL PMID
[2]	Aziz A, Martin-Tanguy J, Larher F (1997). Plasticity of polyamines metabolism associated with high osmotic stress in rape leaf discs and with ethylene treatment. Plant Growth Regulation, 21,153-163. DOI URL
[3]	Becana M, Moran JF, Iturbe-Ormaetxe I (1998). Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant and Soil, 201,137-147. DOI URL
[4]	Bisht SS, Mehrotra SC (1989). Iron cobalt interaction in growth and metabolism of maize (Zea mays). Indian Journal of Agricultural Science, 59,594-596.
[5]	Bouchereau A, Aziz A, Larher F, Martin-Tanguy J (1999). Polyamines and environmental challenges: recent development. Plant Science, 140,103-125. DOI URL
[6]	Chatterjee J, Chatterjee C (2003). Management of phytotoxity of cobalt in tomato by chemical measures. Plant Science, 164,793-801. DOI URL
[7]	Dhindsa RS, Matowe W (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany, 32,79-91.
[8]	Even-Chen Z, Mattoo AK, Goden R (1982). Inhibition of ethylene biosynthesis by aminoethoxyvinylglycine and by polyamines shunts label from 3.4-( ¹⁴C) methionine into spermidine in ages orange peel discs . Plant Physiology, 69,385-388. DOI URL PMID
[9]	Flores HE, Galston AW (1982). Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiology, 69,701-706. URL PMID
[10]	Freedman B, Hutchinson TC (1981). Effect of Heavy Metal Pollution on Plants. Metals in the Environment Vol. 2. Applied Science Publishers, London,35-94.
[11]	Galston AW, Sawhney RK (1990). Polyamines in plant physiology. Plant Physiology, 94,406-410. DOI URL PMID
[12]	Gaspar T, Franck T, Bisbis B, Kevers C, Jouve L, Hausman JF, Dommes J (2002). Concepts in plant stress physiology, application to plant tissue cultures. Plant Growth Regulation, 37,263-285.
[13]	Guevara SR, Massaferro J, Villarosa G, Arribére M, Rizzo A (2002). Heavy metal contamination in sediments of lake Nahuel Huapi, Nahuel Huapi National Park, Northern Patagonia, Argentina. Water, Air and Soil Pollution, 137,21-44.
[14]	Hansen H, Larssen T, Seip HM, Vogt RD (2001). Trace metals in soils at four sites in southern China. Water, Air and Soil Pollution, 130,1721-1726.
[15]	Hoagland DR, Arnon DI (1957). California agriculture experiment station. Circular, 347. URL PMID
[16]	Johnson PR, Ecker JR (1998). The ethylene gas signal transduction pathway: a molecular perspective. Annul Review of Genetics, 32,227-254.
[17]	Kleizaite V, Cesniene T, Rancelis V (2004). The use of cobalt-induced chlorophyll morphoses for studying Co ²⁺ interactions with cysteine and SOD. Plant Science, 167,1249-1256.
[18]	Lau OL, Yang SF (1976). Inhibition of ethylene production by cobaltous ion. Plant Physiology, 58,14-17.
[19]	Lichtenthaler HK (1984). Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymology, 148,350-382.
[20]	Li CZ, Jiao J, Wang GX (2004a). The important roles of reactive oxygen species in the relationship between ethylene and polyamines in the leaves of spring wheat seedlings under root osmotic stress. Plant Science, 166,303-315. DOI URL
[21]	Li CZ, Wei XP, Li W, Wang GX (2004b). Relationship between ethylene and spermidine in the leaves of Glycyrrhiza uralensis seedlings under root osmotic stress . Russian Journal of Plant Physiology, 51,372-378.
[22]	Lin JS, Wang GX (2002). Double CO₂ could improve the drought tolerance better in sensitive cultivars than in tolerant cultivars in spring wheat . Plant Science, 163,627-637.
[23]	Locke JM, Bryce JH, Morris PC (2000). Controlling effects of ethylene perception and biosynthesis inhibitors on germination and seedling growth of barley ( Hordeum vulgare L.) . Journal of Experimental Botany, 51,1843-1849. DOI URL PMID
[24]	Manuel B, Pedro A, Juan JI, Manuel S (1986). Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa . Plant Physiology, 82,1169-1171. DOI URL PMID
[25]	Marschner H (1995). Beneficial mineral elements. In: Marschner H ed. Mineral Nutrition of Higher Plants. Academic Press, Harcourt Brace and Company, London,405-435.
[26]	Martin-Tanguy J (2001). Metabolism and function of polyamines in plants: recent development (New approaches). Plant Growth Regulation, 34,135-148.
[27]	Mizrahi Y, Applewhite PB, Galston AW (1989). Polyamine binding to protein in oat and petunia protoplasts. Plant physiology, 91,738-743. DOI URL PMID
[28]	Pandey S, Ranade SA, Nagar PK, Kumar N (2000). Role of polyamines and ethylene as modulators of plant senescence. Journal of Bioscience, 25,291-299.
[29]	Rauser WE, Samarakoon AB (1980). Vein loading in seedlings of Phaseolus vulgaris exposed to excess cobalt, nickel, and zinc . Plant Physiology, 65,578-583. DOI URL PMID
[30]	Saftner RA, Bald BG (1990). Polyamine levels and tomato fruit development: possible interaction with ethylene. Plant Physiology, 92,547-550. URL PMID
[31]	Samecka-Cymerman A, Kempers AJ (2001). Concentrations of heavy metals and plant nutrients in water, sediments and aquatic macrophytes of Anthropogenic Lakes (former open cut brown coal mines) differing in stage of acidification. The Science of Total Environment, 281,87-98.
[32]	Spychalla JP, Desborough SL (1990). Superoxide dismutase. Plant Physiology, 94,1214-1218. URL PMID
[33]	Tadolini B, Cabrini L, Landi L (1984). Polyamine binding to phospholipid vesicles and inhibition of lipid peroxidation. Biochemical Biophysical Research Communications, 122,550-555. URL PMID
[34]	Tewari RK, Kumar P, Sharma PN, Bisht SS (2002). Modulation of oxidative stress responsive enzymes by excess cobalt. Plant Science, 162,381-388.
[35]	Xu YC, Wang J, Dong X, Li MM, Shan L (2001). Effect of exogenous polyamines on glycolate oxidase activity and active oxygen species accumulation in wheat seedlings under osmotic stress. Israel Journal of Plant Science, 49,173-178.
[36]	Young ND, Galston AW (1983). Putrescine and acid stress. Plant Physiology, 71,767-771. DOI URL PMID