植物生态学报 ›› 2017, Vol. 41 ›› Issue (3): 369-377.DOI: 10.17521/cjpe.2016.0098
出版日期:
2017-03-10
发布日期:
2017-04-12
通讯作者:
万贤崇
作者简介:
* 通信作者Author for correspondence (E-mail:
基金资助:
Jian-Rong GUO, Xian-Chong WAN*()
Online:
2017-03-10
Published:
2017-04-12
Contact:
Xian-Chong WAN
About author:
KANG Jing-yao(1991-), E-mail:
摘要:
为研究土壤盆栽和溶液培养的‘84K’ (Populus alba × P. glandulosa)离体根系的根压及根压昼夜节律, 系统探讨影响根压的因素及它们与根压的关系, 深入了解根压的产生机制及其节律调控, 采用压力转换器法对‘84K’离体根系的根压进行了研究。该研究采用不同时间取样、摘叶、环割等试验处理, 不同土壤温度、昼夜温差/恒温等测定条件研究了离体根压的节律, 并进一步运用化学抑制剂研究根系呼吸代谢、根系导水率对根压的影响, 并测定伤流液的渗透强度及其无机离子和可溶性糖含量日变化以探讨它们和根压昼夜节律的关系。研究发现, ‘84K’离体根系的根压具有昼高夜低的周期节律, 在上午至正午达到峰值, 在20:00达到谷值。不同取样时间、不同培养介质对根压周期性有一定的影响, 但总体昼高夜低没有改变。摘叶、环割、呼吸抑制剂或细胞膜水导抑制剂影响根压峰值的大小, 但对根压的昼夜节律没有明显影响。摘叶、环割或呼吸抑制剂显著地降低了根压峰值, 而水导抑制剂对根压峰值影响不大, 说明根压峰值的大小主要受呼吸作用, 包括呼吸底物的影响, 而根系水导率对根压峰值的影响不大。根压峰值随土壤温度下降而下降、土壤温度还改变根压的昼夜节律。随温度的变化, 最大根压和呼吸速率同步变化, 意味着根压随温度的变化也是呼吸代谢在起作用。根系伤流液渗透强度白天高晚上低。伤流液无机离子含量以及可溶性糖含量的日变化和渗透强度变化一致, 其中无机离子扮演更主要的角色。昼夜温差条件下的根压峰值显著大于昼夜常温条件下的根压峰值。总之, ‘84K’根压呈现较稳定的昼夜周期性, 昼高夜低。根压峰值大小主要受呼吸代谢的调控, 呼吸抑制剂、呼吸代谢底物、影响呼吸作用的温度等都影响‘84K’根压峰值的大小。根系导水率对根压有一定的影响, 但不显著。
郭建荣, 万贤崇. 杨树根压昼夜周期性及其影响因子. 植物生态学报, 2017, 41(3): 369-377. DOI: 10.17521/cjpe.2016.0098
Jian-Rong GUO, Xian-Chong WAN. Circadian rhythm of root pressure in popular and its driving factors. Chinese Journal of Plant Ecology, 2017, 41(3): 369-377. DOI: 10.17521/cjpe.2016.0098
图3 不同温度条件下测定的根压最大值和呼吸速率(平均值±标准偏差, n = 6)。
Fig. 3 The maximum of root pressure and respiration rate measured under different soil temperatures (mean ± SD, n = 6).
图4 昼夜恒温(25 ℃/25 ℃)和昼夜温差(25 ℃/19 ℃)条件下的根压。
Fig. 4 The root pressure under constant temperature during day and night (25 °C/25 °C) and different temperature during day and night (25 °C /19 °C).
图7 抑制剂对根压峰值的影响(平均值±标准偏差, n = 3-6, α = 0.05。CK, 对照; T1, 处理1, 0.1 mmol·L-1 HgCl2; T2, 处理2, 1.0 mmol·L-1 NaN3; T3, 处理3, 1.0 mmol·L-1 NaN3 + 0.1 mmol·L-1 HgCl2)。
Fig. 7 Effects of inhibitors on the maximum of root pressure (mean ± SD, n = 3-6, α = 0.05. CK, Control; T1, treatment 1, 0.1 mmol·L-1 HgCl2; T2, treatment 2, 1.0 mmol·L-1 NaN3; T3, treatment 3, 1.0 mmol·L-1 NaN3 + 0.1 mmol·L-1 HgCl2).
处理剂 Dispose dose | 根系呼吸速率抑制率 Inhibition percentage of root respiration (%) | 根系导水率抑制率 Inhibition percentage of root hydraulic conductance (%) |
---|---|---|
1.0 mmol·L-1 NaN3 | 44.07 ± 15.35a | 41.66 ± 14.83a |
0.1 mmol·L-1 HgCl2 | 23.16 ± 0.01b | 50.94 ± 31.37a |
1.0 mmol·L-1 NaN3 + 0.1 mmol·L-1 HgCl2 | 50.00 ± 4.55a | 44.04 ± 21.49a |
表1 抑制剂对根系呼吸速率和根系导水率的抑制作用(平均值±标准偏差)
Table 1 Effects of inhibitors on root respiration rate and hydraulic conductivity (mean ± SD)
处理剂 Dispose dose | 根系呼吸速率抑制率 Inhibition percentage of root respiration (%) | 根系导水率抑制率 Inhibition percentage of root hydraulic conductance (%) |
---|---|---|
1.0 mmol·L-1 NaN3 | 44.07 ± 15.35a | 41.66 ± 14.83a |
0.1 mmol·L-1 HgCl2 | 23.16 ± 0.01b | 50.94 ± 31.37a |
1.0 mmol·L-1 NaN3 + 0.1 mmol·L-1 HgCl2 | 50.00 ± 4.55a | 44.04 ± 21.49a |
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