Improved method of obtaining micro-core paraffin sections in dendroecological research

doi:10.3724/SP.J.1258.2013.00100

Abstract

Abstract:

Aims Despite numerous dendroclimatological and dendroecological studies conducted over many regions around the world, associated research on the mechanism of how tree species respond to climate change rarely have been reported. In order to explore the climate-growth patterns in detail, observations of cambial activities have been widely used for physiological and ecological insights.
Methods By investigating seasonal dynamics of cambial activities of five different tree species like Sabina przewalskii, Betula albo-sinensis, Pinus tabuliformis, Picea crassifolia, and Picea wilsonii, we improved the pre-existing technique using plant paraffin sections.
Important findings We considered the process of softening and the timing control of dehydration, cleaning, wax-dipping and staining to improve cambial slices and thereby a provide reliable foundation of paraffin sections of lignified samples useful to the domains of dendroclimatology, dendroecology and wood anatomy.

Key words: dendroecology, micro-core, paraffin section, tree-ring

ZHANG Jun-Zhou, GOU Xiao-Hua, ZHAO Zhi-Qian, LIU Wen-Huo, ZHANG Fen, CAO Zong-Ying, ZHOU Fei-Fei. Improved method of obtaining micro-core paraffin sections in dendroecological research[J]. Chin J Plant Ecol, 2013, 37(10): 972-977.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2013.00100

https://www.plant-ecology.com/EN/Y2013/V37/I10/972

Figures/Tables 6

References 20

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步骤 Step	试剂 Reagent	时间 Time
1	30%乙醇 30% ethanol	90-120 min
2	50%乙醇 50% ethanol	90-120 min
3	70%乙醇 70% ethanol	90-120 min
4	85%乙醇 85% ethanol	90-120 min
5	95%乙醇 95% ethanol	60-90 min
6	无水乙醇I Anhydrous ethanol I	60-90 min
7	无水乙醇II Anhydrous ethanol II	60 min
8	二甲苯:乙醇= 1:1 Dimethylbenzene: ethanol = 1:1	60 min
9	二甲苯I Dimethylbenzene I	60 min
10	二甲苯II Dimethylbenzene II	60 min
11	二甲苯:石蜡= 1:1 Dimethylbenzene: paraffin = 1:1	48-72 h
12	石蜡I Paraffin I	120 min
13	石蜡II Paraffin II	60 min
14	石蜡III Paraffin III	30 min

步骤 Step	试剂 Reagent	时间 Time
1	30%乙醇 30% ethanol	90-120 min
2	50%乙醇 50% ethanol	90-120 min
3	70%乙醇 70% ethanol	90-120 min
4	85%乙醇 85% ethanol	90-120 min
5	95%乙醇 95% ethanol	60-90 min
6	无水乙醇I Anhydrous ethanol I	60-90 min
7	无水乙醇II Anhydrous ethanol II	60 min
8	二甲苯:乙醇= 1:1 Dimethylbenzene: ethanol = 1:1	60 min
9	二甲苯I Dimethylbenzene I	60 min
10	二甲苯II Dimethylbenzene II	60 min
11	二甲苯:石蜡= 1:1 Dimethylbenzene: paraffin = 1:1	48-72 h
12	石蜡I Paraffin I	120 min
13	石蜡II Paraffin II	60 min
14	石蜡III Paraffin III	30 min

步骤 Step	试剂 Reagent	染色时间 Staining time
1	二甲苯 Dimethylbenzene	12 min
2	无水乙醇:二甲苯= 1:1 Anhydrous ethanol:dimethylbenzene = 1:1	6 min
3	无水乙醇 Anhydrous ethanol	6 min
4	95%乙醇 95% ethanol	6 min
5	85%乙醇 85% ethanol	6 min
6	70%乙醇 70% ethanol	6 min
7	50%乙醇 50% ethanol	6 min
8	30%乙醇 30% ethanol	6 min
9	水 Water	6 min
10	1%番红水溶液 1% safranine water solution	3-5 h
11	水 Water	60 s
12	30%乙醇 30% ethanol	60 s
13	50%乙醇 50% ethanol	60 s
14	70%乙醇 70% ethanol	60 s
15	85%乙醇 85% ethanol	60 s
16	0.5%固绿乙醇溶液 0.5% fast green ethanol solution	30 s
17	95%乙醇 95% ethanol	60 s
18	无水乙醇 Anhydrous ethanol	60 s
19	无水乙醇:二甲苯=1:1 Anhydrous ethanol:dimethylbenzene = 1:1	60 s
20	二甲苯 Dimethylbenzene	60 s

步骤 Step	试剂 Reagent	染色时间 Staining time
1	二甲苯 Dimethylbenzene	12 min
2	无水乙醇:二甲苯= 1:1 Anhydrous ethanol:dimethylbenzene = 1:1	6 min
3	无水乙醇 Anhydrous ethanol	6 min
4	95%乙醇 95% ethanol	6 min
5	85%乙醇 85% ethanol	6 min
6	70%乙醇 70% ethanol	6 min
7	50%乙醇 50% ethanol	6 min
8	30%乙醇 30% ethanol	6 min
9	水 Water	6 min
10	1%番红水溶液 1% safranine water solution	3-5 h
11	水 Water	60 s
12	30%乙醇 30% ethanol	60 s
13	50%乙醇 50% ethanol	60 s
14	70%乙醇 70% ethanol	60 s
15	85%乙醇 85% ethanol	60 s
16	0.5%固绿乙醇溶液 0.5% fast green ethanol solution	30 s
17	95%乙醇 95% ethanol	60 s
18	无水乙醇 Anhydrous ethanol	60 s
19	无水乙醇:二甲苯=1:1 Anhydrous ethanol:dimethylbenzene = 1:1	60 s
20	二甲苯 Dimethylbenzene	60 s