Causes of decreasing mitochondrial respiration under light in four crops

doi:10.3724/SP.J.1258.2014.00105

Abstract

Abstract:

Aims Despite the increasing attention given to the rate of mitochondrial respiration under light (R_d), considerable confusion persists over whether mitochondrial respiration in the dark (R_n) is inhibited by light and whether R_d is affected by light intensity. The objective of this study is to test the hypotheses: 1)R_n is not inhibited by light; 2) the rate of R_d changes with light intensity; and 3) the photosynthetic refixation of CO₂ produced by R_n accounts for the apparent disparity between R_d and R_n.
Methods In the present study, 0.02 mol·mol^-1 O₂ (i.e. 2% O₂) was used to saturate R_n and to inhibit photorespiration (R_p). By using combined gas exchange measurements and a low O₂ (2% O₂) method, the post-illumination CO₂ release rate of R_n, photosynthetic rate (P_n) in response to photosynthetically active radiation (PAR) in 2% O₂ at either 380 or 0 μmol·mol^-1 CO₂, of C₃ (Triticum aestivum and Glycine max) and C₄(Zea mays and Amaranthus hypochondriacus) plants, were measured.
Important finding R_n was not inhibited by light. At 2% O₂ and 0 μmol·mol^-1 CO₂, the measured parameters could be used to accurately estimate R_d when CO₂ concentration was set for 0 μmol·mol^-1. R_d decreased with increasing light intensity. Although R_d was lower in the dark, this could be accounted for by photosynthetic re-fixing of respiratory CO₂. For all plants tested, CO₂recovery rates increased with increasing light intensity (from 50 and 2 000 μmol·m^-2·s^-1).

Key words: CO₂ re-fixed, inhibition, mitochondrial respiration in the light, photorespiration

KANG Hua-Jing, LI Hong, QUAN Wei, OUYANG Zhu. Causes of decreasing mitochondrial respiration under light in four crops[J]. Chin J Plant Ecol, 2014, 38(10): 1110-1116.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2014.00105

https://www.plant-ecology.com/EN/Y2014/V38/I10/1110

Figures/Tables 6

Fig. 1 Light response curves of photosynthesis for wheat (A), bean (B), maize (C) and three-colored amaranth (D) (mean ± SD). Dots (●) represent measured data, and black line represents fitted data. PAR, photosynthetically active radiation; Pn, net photosynthetic rate.

Fig. 2 Dynamics of stomatal conductance (Gs) after light being turned off for wheat (A), bean (B), maize (C) and three-colored amaranth (D) at 380 μmol·mol-1 CO2and 2% O2(mean ± SD).

Fig. 3 Dynamics of mitochondrial respiration (Rn) after light being turned off for wheat (A), bean (B), maize (C) and three-colored amaranth (D) at a CO2 concentration of 380 μmol·mol-1 and 2% O2(mean ± SD).

Fig. 4 Leaf chamber CO2 concentration (Cs) and intercellular CO2 concentration (Ci) for wheat (A), bean (B), maize (C) and three-colored amaranth (D) at a CO2 concentration of 0 μmol·mol-1 and 2% O2(mean ± SD). PAR, photosynthetically active radiation.

Fig. 5 Net photosynthetic rate (Pn) for wheat (A), bean (B), maize (C) and three-colored amaranth (D) at a CO2 concentration of 0 μmol·mol-1 and 2% O2(mean ± SD).

Fig. 6 Recovery of respiratory CO2 for wheat and bean (left), and maize and three-colored amaranth (right) at a CO2 concentration of 0 μmol·mol-1 and 2% O2under different photosynthetically active radication (PAR) (mean ± SD).

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