Soil fungi distribution patterns under different levels of disturbance in the wetland of Napahai Plateau

doi:10.3724/SP.J.1258.2014.00112

Abstract

Abstract: Aims The study was undertaken to reveal the effects of disturbance on carbon, nitrogen and soil fungi distribution patterns in Napahai wetland.Methods Changes in soil carbon and nitrogen and ecological characteristics of soil fungi were studied across wetlands of varying levels of disturbance, including primitive marsh (non-disturbance), swampy meadow (low disturbance), meadow (moderate disturbance), and cultivated wetland (high disturbance). Important findings (1) In both upper and lower soil layers, soil organic matter (SOM), total nitrogen (TN), C:N and pH significantly differed (p < 0.01) among the four wetland types; SOM and TN decreased with increasing level of disturbance. (2) Following the culture on PDA medium, the abundance of soil fungi was greater in the upper layer than in the lower layer within the same type of wetland soil; with increasing level of disturbance, the abundance of fungi gradually increased, and the abundance of fungi was negatively correlated with pH value, SOM and TN, and positively correlated with C:N (2-tail test, p < 0.05). (3) Phylogenetic analysis shows that the soil fungi in Napahai wetland occur as Ascomycota, Basidiomycota, and Zygomycota, with Ascomycota being the dominant type and playing a key role in decomposition of soil carbon and nitrogen in plateau wetland.

Key words: carbon, disturbance intensity, nitrogen, plateau wetland, soil fungi

CAO Ping-Lin,LU Mei,TIAN Kun,LÜ Si-Tong,YANG Hong-Sheng,YAO Xi,LI Li-Ping,YUE Hai-Tao. Soil fungi distribution patterns under different levels of disturbance in the wetland of Napahai Plateau[J]. Chin J Plant Ecol, 2014, 38(11): 1166-1173.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2014.00112

https://www.plant-ecology.com/EN/Y2014/V38/I11/1166

Figures/Tables 6

References 31

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土壤理化指标 Soil physicochemical variable	土层 Soil layer (cm)	垦后湿地(重度干扰) Cultivated wetland (high disturbance)	草甸(中度干扰) Meadow (moderate disturbance)	沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	原生沼泽(无干扰) Marsh (non-disturbance)
有机质 Organic matter (g·kg^-1)	0-20	64.60 ± 6.38^a	72.52 ± 2.73^b	85.01 ± 2.06^c	90.98 ± 3.99^d
有机质 Organic matter (g·kg^-1)	20-40	59.48 ± 2.82^a	73.74 ± 2.54^b	87.23 ± 0.85^c	92.12 ± 3.02^d
全氮 Total nitrogen (g·kg^-1)	0-20	0.74 ± 0.01^a	1.15 ± 0.03^b	1.46 ± 0.02^c	2.51 ± 0.13^d
全氮 Total nitrogen (g·kg^-1)	20-40	0.66 ± 0.03^a	0.84 ± 0.22^a	1.43 ± 0.09^b	1.95 ± 0.08^c
碳氮比 C:N	0-20	87.44 ± 8.70^a	62.87 ± 2.60^b	58.26 ± 2.11^b	36.26 ± 3.12^b
碳氮比 C:N	20-40	90.76 ± 7.43^a	29.77 ± 6.95^b	40.09 ± 2.13^c	47.31 ± 2.91^d
pH值 pH value	0-20	7.61 ± 0.29^a	5.65 ± 0.12^b	7.82 ± 0.16^a	7.84 ± 0.07^a
pH值 pH value	20-40	7.50 ± 0.20^a	6.97 ± 0.51^b	7.87 ± 0.23^c	7.91 ± 0.07^c

土壤理化指标 Soil physicochemical variable	土层 Soil layer (cm)	垦后湿地(重度干扰) Cultivated wetland (high disturbance)	草甸(中度干扰) Meadow (moderate disturbance)	沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	原生沼泽(无干扰) Marsh (non-disturbance)
有机质 Organic matter (g·kg^-1)	0-20	64.60 ± 6.38^a	72.52 ± 2.73^b	85.01 ± 2.06^c	90.98 ± 3.99^d
有机质 Organic matter (g·kg^-1)	20-40	59.48 ± 2.82^a	73.74 ± 2.54^b	87.23 ± 0.85^c	92.12 ± 3.02^d
全氮 Total nitrogen (g·kg^-1)	0-20	0.74 ± 0.01^a	1.15 ± 0.03^b	1.46 ± 0.02^c	2.51 ± 0.13^d
全氮 Total nitrogen (g·kg^-1)	20-40	0.66 ± 0.03^a	0.84 ± 0.22^a	1.43 ± 0.09^b	1.95 ± 0.08^c
碳氮比 C:N	0-20	87.44 ± 8.70^a	62.87 ± 2.60^b	58.26 ± 2.11^b	36.26 ± 3.12^b
碳氮比 C:N	20-40	90.76 ± 7.43^a	29.77 ± 6.95^b	40.09 ± 2.13^c	47.31 ± 2.91^d
pH值 pH value	0-20	7.61 ± 0.29^a	5.65 ± 0.12^b	7.82 ± 0.16^a	7.84 ± 0.07^a
pH值 pH value	20-40	7.50 ± 0.20^a	6.97 ± 0.51^b	7.87 ± 0.23^c	7.91 ± 0.07^c

湿地类型 Wetland type	土层 Soil layer (cm)	平板菌落 No. of plate culture count	菌落单元数(CFU·g^-1)(平均值±标准误差) Numbers of colony-forming units (mean ± SE)
垦后湿地(重度干扰) Cultivated wetland (high disturbance)	0-20	23.30	(23.30 ± 0.63) × 10^3a
垦后湿地(重度干扰) Cultivated wetland (high disturbance)	20-40	15.35	(15.35 ± 0.20) × 10^3a
草甸(中度干扰) Meadow (moderate disturbance)	0-20	15.98	(15.98 ± 0.14) × 10^3b
草甸(中度干扰) Meadow (moderate disturbance)	20-40	12.44	(12.44 ± 0.21) × 10^3b
沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	0-20	5.06	(5.06 ± 0.11) × 10^3c
沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	20-40	2.72	(2.72 ± 0.10) × 10^3c
原生沼泽(无干扰) Marsh (non-disturbance)	0-20	1.79	(1.79 ± 0.06) × 10^3d
原生沼泽(无干扰) Marsh (non-disturbance)	20-40	1.00	(1.00 ± 0.08) × 10^3d

湿地类型 Wetland type	土层 Soil layer (cm)	平板菌落 No. of plate culture count	菌落单元数(CFU·g^-1)(平均值±标准误差) Numbers of colony-forming units (mean ± SE)
垦后湿地(重度干扰) Cultivated wetland (high disturbance)	0-20	23.30	(23.30 ± 0.63) × 10^3a
垦后湿地(重度干扰) Cultivated wetland (high disturbance)	20-40	15.35	(15.35 ± 0.20) × 10^3a
草甸(中度干扰) Meadow (moderate disturbance)	0-20	15.98	(15.98 ± 0.14) × 10^3b
草甸(中度干扰) Meadow (moderate disturbance)	20-40	12.44	(12.44 ± 0.21) × 10^3b
沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	0-20	5.06	(5.06 ± 0.11) × 10^3c
沼泽化草甸(轻度干扰) Swampy meadow (low disturbance)	20-40	2.72	(2.72 ± 0.10) × 10^3c
原生沼泽(无干扰) Marsh (non-disturbance)	0-20	1.79	(1.79 ± 0.06) × 10^3d
原生沼泽(无干扰) Marsh (non-disturbance)	20-40	1.00	(1.00 ± 0.08) × 10^3d

	全氮 Total nitrogen	有机质 Organic matter	碳氮比 C:N	真菌数量 No. of fungi	pH
全氮 Total nitrogen	1
有机质 Organic matter	0.852^**	1
碳氮比 C:N	-0.932^**	-0.772^**	1
真菌数量 No. of fungi	-0.887^**	-0.936^*	0.893^**	1
pH	0.344^*	0.358^*	-0.150	-0.382^*	1