Chin J Plan Ecolo ›› 2010, Vol. 34 ›› Issue (4): 438-443.doi: 10.3773/j.issn.1005-264x.2010.04.009

• Research Articles • Previous Articles     Next Articles

Effect of media and light on asymbiotic germination of Malaxis monophyllos seeds

WANG Rui-Xia1,4;HE Ming-Gao3;and SONG Song-Quan2*   

  1. 1Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China;
    2Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
    3Institute of Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan571737, China; and
    4Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2009-03-30 Revised:2009-05-06 Online:2010-04-01 Published:2010-04-01
  • Contact: SONG Song-Quan

Abstract: Aims Extensive collection, environmental deterioration, slow seed germination and low germination percentage have seriously degraded the natural resources of Malaxis monophyllos, resulting in a vulnerable situation for the species. Our objective is to establish an asymbiotic germination system of M. monophyllos seeds to protect and propagate the species.
Methods Seed morphology was investigated by optical microscope and scanning electron microscope. We used Knudson C medium supplemented with 2% sucrose, 3% activated charcoal and 0.8% agar as the basic medium to investigate the effects of α-naphthalene acetic acid (NAA), 6 benzylaminopurine (6-BA), coconut milk, meshed banana, meshed potato and light on seed germination via the orthogonal test.
Important findings Periclinal walls of testa cells were smooth and had no veins, but anticlinal walls were thick and had clear surface patterns. Through the orthogonal test and redundancy analysis, an optimal formula for asymbiotic seed germination was determined: the medium supplemented with 4.5 mg·L–1 NAA, 10 mg·L–1 6-BA, 8% meshed banana and 3% meshed potato in the light (12 h light/12 h dark at 20 μmol·m–2·s–1 of light intensity). Seed germination was > 90% with this formula.

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