Chin J Plant Ecol ›› 2010, Vol. 34 ›› Issue (4): 438-443.DOI: 10.3773/j.issn.1005-264x.2010.04.009
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WANG Rui-Xia1,4, HE Ming-Gao3, SONG Song-Quan2,*()
Received:
2009-03-30
Accepted:
2009-05-15
Online:
2010-03-30
Published:
2010-04-01
Contact:
SONG Song-Quan
WANG Rui-Xia, HE Ming-Gao, SONG Song-Quan. Effect of media and light on asymbiotic germination of Malaxis monophyllos seeds[J]. Chin J Plant Ecol, 2010, 34(4): 438-443.
水平 Level | 因素 Factor | ||||
---|---|---|---|---|---|
A, NAA (mg·L-1) | B, 6-BA (mg·L-1) | C, 椰乳 Coconut milk (%, v/v) | D, 香蕉泥 Meshed banana (%, w/v) | E, 土豆泥 Meshed potato (%, w/v) | |
1 | 0 | 0 | 0 | 0 | 0 |
2 | 0.5 | 1 | 4 | 4 | 3 |
3 | 1.5 | 3 | 8 | 8 | 6 |
4 | 4.5 | 10 | 10 | 10 | 12 |
Table 1 Factors and levels for orthogonal test
水平 Level | 因素 Factor | ||||
---|---|---|---|---|---|
A, NAA (mg·L-1) | B, 6-BA (mg·L-1) | C, 椰乳 Coconut milk (%, v/v) | D, 香蕉泥 Meshed banana (%, w/v) | E, 土豆泥 Meshed potato (%, w/v) | |
1 | 0 | 0 | 0 | 0 | 0 |
2 | 0.5 | 1 | 4 | 4 | 3 |
3 | 1.5 | 3 | 8 | 8 | 6 |
4 | 4.5 | 10 | 10 | 10 | 12 |
处理Treatment | A | B | C | D | E | 光照 Light | 黑暗 Darkness | |||
---|---|---|---|---|---|---|---|---|---|---|
萌发率Germination (%) | 圆球茎诱导率Protocorm (%) | 萌发率 Germination (%) | 圆球茎诱导率Protocorm (%) | |||||||
1 | 1 | 1 | 1 | 1 | 1 | 10.75 ± 1.75fg | 0.00 ± 0.00d | 73.00 ± 2.00b | 7.61 ± 1.28a | |
2 | 1 | 2 | 2 | 2 | 2 | 45.00 ± 3.50c | 17.97 ± 2.51a | 52.50 ± 3.50d | 1.34 ± 1.34bc | |
3 | 1 | 3 | 3 | 3 | 3 | 40.00 ± 6.00cd | 3.64 ± 0.70bcd | 36.50 ± 1.50e | 0.00 ± 0.00c | |
4 | 1 | 4 | 4 | 4 | 4 | 23.00 ± 4.00ef | 0.00 ± 0.00d | 74.00 ± 4.00b | 0.00 ± 0.00c | |
5 | 2 | 1 | 2 | 3 | 4 | 79.00 ± 3.00b | 7.89 ± 0.65abc | 76.50 ± 2.50ab | 0.00 ± 0.00c | |
6 | 2 | 2 | 1 | 4 | 3 | 3.50 ± 2.50g | 0.00 ± 0.00d | 81.00 ± 5.50ab | 4.25 ± 0.75ab | |
7 | 2 | 3 | 4 | 1 | 2 | 20.50 ± 3.50ef | 0.69 ± 0.69cd | 78.67 ± 1.67ab | 3.28 ± 0.04bc | |
8 | 2 | 4 | 3 | 2 | 1 | 40.75 ± 3.75cd | 4.39 ± 1.02abcd | 2.50 ± 1.50g | 0.00 ± 0.00c | |
9 | 3 | 1 | 3 | 4 | 2 | 10.75 ± 2.50g | 0.00 ± 0.00d | 2.00 ± 1.00g | 0.00 ± 0.00c | |
10 | 3 | 2 | 4 | 3 | 1 | 19.67 ± 3.67ef | 16.96 ± 4.46ab | 15.50 ± 4.00f | 0.00 ± 0.00c | |
11 | 3 | 3 | 1 | 2 | 4 | 86.25 ± 4.75ab | 7.61 ± 3.93abc | 85.25 ± 2.25ab | 0.00 ± 0.00c | |
12 | 3 | 4 | 2 | 1 | 3 | 28.25 ± 2.75de | 4.55 ± 1.33abcd | 2.50 ± 1.00g | 0.00 ± 0.00c | |
13 | 4 | 1 | 4 | 2 | 3 | 18.50 ± 2.50ef | 0.00 ± 0.00d | 83.25 ± 3.75ab | 4.47 ± 0.70ab | |
14 | 4 | 2 | 3 | 1 | 4 | 36.50 ± 1.00cd | 6.23 ± 2.23abc | 61.50 ± 3.50c | 2.74 ± 1.88abc | |
15 | 4 | 3 | 2 | 4 | 1 | 30.25 ± 0.75de | 1.61 ± 1.61cd | 8.75 ± 1.25fg | 0.00 ± 0.00c | |
16 | 4 | 4 | 1 | 3 | 2 | 93.25 ± 2.25a | 8.77 ± 5.11abc | 89.50 ± 2.00a | 5.02 ± 0.45ab |
Table 2 Orthogonal test L16 (4)5 of germination of Malaxis monophyllos seeds
处理Treatment | A | B | C | D | E | 光照 Light | 黑暗 Darkness | |||
---|---|---|---|---|---|---|---|---|---|---|
萌发率Germination (%) | 圆球茎诱导率Protocorm (%) | 萌发率 Germination (%) | 圆球茎诱导率Protocorm (%) | |||||||
1 | 1 | 1 | 1 | 1 | 1 | 10.75 ± 1.75fg | 0.00 ± 0.00d | 73.00 ± 2.00b | 7.61 ± 1.28a | |
2 | 1 | 2 | 2 | 2 | 2 | 45.00 ± 3.50c | 17.97 ± 2.51a | 52.50 ± 3.50d | 1.34 ± 1.34bc | |
3 | 1 | 3 | 3 | 3 | 3 | 40.00 ± 6.00cd | 3.64 ± 0.70bcd | 36.50 ± 1.50e | 0.00 ± 0.00c | |
4 | 1 | 4 | 4 | 4 | 4 | 23.00 ± 4.00ef | 0.00 ± 0.00d | 74.00 ± 4.00b | 0.00 ± 0.00c | |
5 | 2 | 1 | 2 | 3 | 4 | 79.00 ± 3.00b | 7.89 ± 0.65abc | 76.50 ± 2.50ab | 0.00 ± 0.00c | |
6 | 2 | 2 | 1 | 4 | 3 | 3.50 ± 2.50g | 0.00 ± 0.00d | 81.00 ± 5.50ab | 4.25 ± 0.75ab | |
7 | 2 | 3 | 4 | 1 | 2 | 20.50 ± 3.50ef | 0.69 ± 0.69cd | 78.67 ± 1.67ab | 3.28 ± 0.04bc | |
8 | 2 | 4 | 3 | 2 | 1 | 40.75 ± 3.75cd | 4.39 ± 1.02abcd | 2.50 ± 1.50g | 0.00 ± 0.00c | |
9 | 3 | 1 | 3 | 4 | 2 | 10.75 ± 2.50g | 0.00 ± 0.00d | 2.00 ± 1.00g | 0.00 ± 0.00c | |
10 | 3 | 2 | 4 | 3 | 1 | 19.67 ± 3.67ef | 16.96 ± 4.46ab | 15.50 ± 4.00f | 0.00 ± 0.00c | |
11 | 3 | 3 | 1 | 2 | 4 | 86.25 ± 4.75ab | 7.61 ± 3.93abc | 85.25 ± 2.25ab | 0.00 ± 0.00c | |
12 | 3 | 4 | 2 | 1 | 3 | 28.25 ± 2.75de | 4.55 ± 1.33abcd | 2.50 ± 1.00g | 0.00 ± 0.00c | |
13 | 4 | 1 | 4 | 2 | 3 | 18.50 ± 2.50ef | 0.00 ± 0.00d | 83.25 ± 3.75ab | 4.47 ± 0.70ab | |
14 | 4 | 2 | 3 | 1 | 4 | 36.50 ± 1.00cd | 6.23 ± 2.23abc | 61.50 ± 3.50c | 2.74 ± 1.88abc | |
15 | 4 | 3 | 2 | 4 | 1 | 30.25 ± 0.75de | 1.61 ± 1.61cd | 8.75 ± 1.25fg | 0.00 ± 0.00c | |
16 | 4 | 4 | 1 | 3 | 2 | 93.25 ± 2.25a | 8.77 ± 5.11abc | 89.50 ± 2.00a | 5.02 ± 0.45ab |
Fig. 3 Redundancy analysis ordination figure of 6 factors affecting germination of Malaxis monophyllos seeds. Eigenvalues: axis 1 = 0.301 (p = 0.01), axis 2 = 0.03; The first two axes cumulatively accounted for 30.4% of the overall variation (p ≤ 0.01); Species (dashed line arrow) and environmental (solid line arrow) correlations for the first two canonical axes were 0.556 and 0.335, respectively.
Fig. 4 The range analysis on the germination in orthogonal design test. A, NAA; B, 6-BA; C, coconut milk; D, meshed banana; E, meshed potato; Kj, means on the according levels.
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