Chin J Plan Ecolo ›› 2010, Vol. 34 ›› Issue (6): 704-712.doi: 10.3773/j.issn.1005-264x.2010.06.010

• Research Articles • Previous Articles     Next Articles

Estimating leaf nitrogen content with near infrared reflectance spectroscopy in rice

ZHANG Yu-Sen; YAO Xia; TIAN Yong-Chao; CAO Wei-Xing; and ZHU Yan*   

  1. Jiangsu Key Laboratory for Information Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-07-07 Online:2010-06-01 Published:2010-06-01
  • Contact: ZHU Yan

Abstract: Aim Our primary objective was to establish an effective method of near infrared reflectance spectroscopy (NIRS) for estimating leaf nitrogen content in rice, which would help with nitrogen diagnosis and dressing fertilization in rice production.
Methods Using the techniques of partial least square (PLS), principal component regression (PCR) and stepwise multiple linear regression (SMLR), we established four NIRS-based models for estimating nitrogen content (NC) in fresh leaf and leaf powder of rice cultivars under varied nitrogen application rates.
Important findings The coefficient of determination (RC2) and root mean square error for calibration (RMSEC) of NC models with fresh leaf were 0.940 and 0.226, respectively, whereas the RC2 and RMSEC of NC models with leaf powder were 0.977 and 0.136, respectively. We tested the accuracy of models with independent experiment datasets by the determination coefficient (RCV2) and root mean square error of cross-validation (RMSECV), and the determination coefficient (RV2) and root mean square error of external validation (RMSEP). With fresh leaf, the RCV2 and RMSECV of NC models were 0.866 and 0.243, respectively, while the RV2 was >0.800 and RMSEP was <0.500. With leaf powder, the RCV2 and RMSECV of NC models were 0.900 and 0.202, respectively, whereas the RV2 and RMSEP were 0.944 and 0.142, respectively. Overall, the performance of the models with leaf powder is better than that with fresh leaf in rice.

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[1] Cui Gao;Yuxia Chen;Ying Bao;Min Feng;Anming Lu. Studies on Sexual Organs and Embryological Development Morphology of Speirantha gardenii (Convallariaceae)[J]. Chin Bull Bot, 2010, 45(06): 705 -712 .
[2] Jiang Gao-ming. The Impact of Globae Increasing of CO2 on Plants[J]. Chin Bull Bot, 1995, 12(04): 1 -7 .
[3] Zhang Jun Han Bi-wen. Advance in the Study of Histochemical Localization for[J]. Chin Bull Bot, 1995, 12(专辑3): 131 -142 .
[4] Tang Yan-cheng. A Short Guide to the International Code of Botanical Nomenclature V.[J]. Chin Bull Bot, 1984, 2(04): 51 -57 .
[5] Xu Ji. The Protective Protein of Nitrogenase Against Oxygen Damage-Fe-S Protein[J]. Chin Bull Bot, 1986, 4(12): 1 -4 .
[6] . [J]. Chin Bull Bot, 2001, 18(05): 633 .
[7] Huang Zhao-xiang;Zheng Zhen-gui and Zhu Du. Ecological Effect of Taxodium ascendens-Oryza sativa Ecosystem(I) The Growing Characteristic of Taxodium Ascendens in the Ecosystem[J]. Chin Bull Bot, 1996, 13(02): 48 -51 .
[8] GU Rui-Sheng;LIU Qun-Lu;CHEN Xue-Mei and JIANG Xiang-Ning. Comparison and Optimization of the Methods on Protein Extraction and SDS-PAGE in Woody Plants[J]. Chin Bull Bot, 1999, 16(02): 171 -177 .
[9] Jiang Gao-ming. LI-6400 Portable Photosynthesis System: Principle, Function, Basic Operation and Main Problems and Solutions During Measurement[J]. Chin Bull Bot, 1996, 13(增刊): 72 -76 .
[10] Li Ling;Luo Yun-xiu;He Jian-hui and Pan Rui-chi. Promoting the Formation of Adventitious Roots in Cutting of Some Woody Plants by GL Reagent[J]. Chin Bull Bot, 1996, 13(增刊): 63 -65 .