Chin J Plant Ecol ›› 2012, Vol. 36 ›› Issue (5): 382-392.DOI: 10.3724/SP.J.1258.2012.00382
Special Issue: 青藏高原植物生态学:植物-土壤-微生物
• Research Articles • Previous Articles Next Articles
LIU Yang, ZHANG Jian*(), YAN Bang-Guo, HUANG Xu, XU Zhen-Feng, WU Fu-Zhong
Published:
2012-05-04
Contact:
ZHANG Jian
LIU Yang, ZHANG Jian, YAN Bang-Guo, HUANG Xu, XU Zhen-Feng, WU Fu-Zhong. Seasonal dynamics in soil microbial biomass carbon and nitrogen and microbial quantity in a forest-alpine tundra ecotone, Eastern Qinghai-Tibetan Plateau, China[J]. Chin J Plant Ecol, 2012, 36(5): 382-392.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2012.00382
样带 Belt transect | 暗针叶林Dark-conifer forest | 林线Timberline | 树线 Treeline | 密灌丛 Dense shrub | 疏灌丛 Sparse shrub | 高山草甸 Alpine meadow |
---|---|---|---|---|---|---|
海拔 Elevation (m) | 3 900 | 3 975 | 4 050 | 4 105 | 4 135 | 4 200 |
坡向 Slope aspect (°) | EN 5 | EN 4 | EN 5 | EN 5 | EN 3 | EN 3 |
坡度 Slope degree (°) | 32 | 34 | 40 | 36 | 38 | 34 |
木本植物平均高度 Wood average height (m) | 22.0 | 14.0 | 8.0 | 5.0 | 1.5 | – |
草本植物平均高度 Herbage average height (cm) | 10 | 20 | 50 | 35 | 20 | 10 |
郁闭度 Crown density (%) | 80 | 75 | 70 | 65 | 20 | – |
草本植物盖度 Cover degree of herb (%) | 20 | 30 | 40 | 60 | 90 | 85 |
苔藓厚度 Thickness of moss (cm) | 10.0 | 4.5 | 4.0 | 1.8 | 0.5 | 0.6 |
枯落物厚度 Thickness of litter (cm) | 9.0 | 8.0 | 5.8 | 4.5 | 2.5 | 1.2 |
Table 1 Basic characteristics of alpine forest-tundra ecotone
样带 Belt transect | 暗针叶林Dark-conifer forest | 林线Timberline | 树线 Treeline | 密灌丛 Dense shrub | 疏灌丛 Sparse shrub | 高山草甸 Alpine meadow |
---|---|---|---|---|---|---|
海拔 Elevation (m) | 3 900 | 3 975 | 4 050 | 4 105 | 4 135 | 4 200 |
坡向 Slope aspect (°) | EN 5 | EN 4 | EN 5 | EN 5 | EN 3 | EN 3 |
坡度 Slope degree (°) | 32 | 34 | 40 | 36 | 38 | 34 |
木本植物平均高度 Wood average height (m) | 22.0 | 14.0 | 8.0 | 5.0 | 1.5 | – |
草本植物平均高度 Herbage average height (cm) | 10 | 20 | 50 | 35 | 20 | 10 |
郁闭度 Crown density (%) | 80 | 75 | 70 | 65 | 20 | – |
草本植物盖度 Cover degree of herb (%) | 20 | 30 | 40 | 60 | 90 | 85 |
苔藓厚度 Thickness of moss (cm) | 10.0 | 4.5 | 4.0 | 1.8 | 0.5 | 0.6 |
枯落物厚度 Thickness of litter (cm) | 9.0 | 8.0 | 5.8 | 4.5 | 2.5 | 1.2 |
Fig. 1 Dynamics of average daily air temperature (A) and soil temperature at 10 cm depth by month (B) from May to October, 2009 in the forest-alpine tundra ecotone. AM, alpine meadow; DCF, dark-conifer forest; DS, dense shrub; SS, sparse shrub; Ti, timberline; Tr, treeline.
Fig. 2 Seasonal changes of soil microbial biomass carbon (A), nitrogen (B) and C: N (C), as well as quantity of cultivate bacteria (D), fungi (E) and actinomycetes (F) of soil in forest-alpine tundra ecotone (mean ± SD). Lower case letters indicate significant differences among different vegetations within same season (p < 0.05); upper case letters indicate significant differences among different seasons within same vegetation (p < 0.05). EGS, early in the growing season; LGS, late in the growing season; MGS, middle in the growing season. AM, alpine meadow; DCF, dark-conifer forest; DS, dense shrub; SS, sparse shrub; Ti, timberline; Tr, treeline.
变量 Variable | 植被 Vegetation | 季节 Season | 植被×季节 Vegetation×season | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
df | F | p | df | F | p | df | F | p | |||
微生物生物量碳 MBC | 5 | 20.64 | <0.001 | 2 | 481.75 | <0.001 | 10 | 14.40 | <0.001 | ||
微生物生物量氮 MBN | 5 | 120.48 | <0.001 | 2 | 7.44 | 0.004 | 10 | 20.21 | <0.001 | ||
微生物生物量碳氮比 MBC/MBN | 5 | 26.53 | <0.001 | 2 | 541.92 | <0.001 | 10 | 17.07 | <0.001 | ||
细菌 Bacteria | 5 | 86.20 | <0.001 | 2 | 22.59 | <0.001 | 10 | 7.16 | <0.001 | ||
真菌 Fungi | 5 | 55.36 | <0.001 | 2 | 166.69 | <0.001 | 10 | 14.13 | <0.001 | ||
放线菌 Actinomycetes | 5 | 51.18 | <0.001 | 2 | 58.46 | <0.001 | 10 | 14.37 | <0.001 |
Table 2 Results of two-way ANOVA for the analysis of the main effects of vegetation types (from dark-conifer forest to alpine meadow), season (three times collections from May to October) and their interactions for soil microbial biomass C, N and microbe quantity determined in 10-cm depth soil layer in forest-alpine tundra ecotone
变量 Variable | 植被 Vegetation | 季节 Season | 植被×季节 Vegetation×season | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
df | F | p | df | F | p | df | F | p | |||
微生物生物量碳 MBC | 5 | 20.64 | <0.001 | 2 | 481.75 | <0.001 | 10 | 14.40 | <0.001 | ||
微生物生物量氮 MBN | 5 | 120.48 | <0.001 | 2 | 7.44 | 0.004 | 10 | 20.21 | <0.001 | ||
微生物生物量碳氮比 MBC/MBN | 5 | 26.53 | <0.001 | 2 | 541.92 | <0.001 | 10 | 17.07 | <0.001 | ||
细菌 Bacteria | 5 | 86.20 | <0.001 | 2 | 22.59 | <0.001 | 10 | 7.16 | <0.001 | ||
真菌 Fungi | 5 | 55.36 | <0.001 | 2 | 166.69 | <0.001 | 10 | 14.13 | <0.001 | ||
放线菌 Actinomycetes | 5 | 51.18 | <0.001 | 2 | 58.46 | <0.001 | 10 | 14.37 | <0.001 |
[1] | ACIA Secretariat (2005). Arctic Climate Impact Assessment. Cambridge University Press, Cambridge, UK. 1042. |
[2] | Alftine KJ, Malanson GP, Fagre DB (2003). Feedback-driven response to multidecadal climatic variability at an Alpine treeline. Physical Geography, 24, 520-535. |
[3] |
Balser TC, Firestone MK (2004). Linking microbial community composition and soil processes in a California annual grassland and mixed-conifer forest. Biogeochemistry, 73, 395-415.
DOI URL |
[4] | Bardgett RD, Lovell RD, Hobbs PJ, Jarvis SC (1999). Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands. Soil Biology & Biochemistry, 31, 1021-1030. |
[5] | Bardgett RD, McAlister E (1999). The measurement of soil fungal: bacterial biomass ratios as an indicator of ecosystem self-regulation in temperate meadow grasslands. Biology and Fertility of Soils, 19, 282-290. |
[6] | Björk RG, Björkman MP, Andersson MX, Klemedtsson L (2008). Temporal variation in soil microbial communities in Alpine tundra. Soil Biology & Biochemistry, 40, 266-268. |
[7] | Butler DR, Malanson GP, Cairns DM (1994). Stability of alpine treeline in Northern Montana, USA. Phytocoenologia, 22, 485-500. |
[8] | Callaway RM, Brooker RW, Choler P, Kikvidze Z, Lortie CJ, Michalet R, Paolini L, Pugnaire FL, Newingham B, Asc- hehoug ET, Armas C, Kikodze D, Cook BJ (2002). Posi- tive interactions among alpine plants increase with stress. Nature, 417, 844-848. |
[9] | Chu HY, Grogan P (2010). Soil microbial biomass, nutrient availability and nitrogen mineralization potential among vegetation-types in a low arctic tundra landscape. Plant and Soil, 329, 411-420. |
[10] | Devi NB, Yadava PS (2006). Seasonal dynamics in soil microbial biomass C, N and P in a mixed-oak forest ecosystem of Manipur, North-east India. Applied Soil Ecology, 31, 220-227. |
[11] | Díaz-Varela RA, Colombo R, Meroni M, Calvo-Iglesias MS, Buffoni A, Tagliaferri A (2010). Spatio-temporal analysis of alpine ecotones: a spatial explicit model targeting altitudinal vegetation shifts. Ecological Modelling, 221, 621-633. |
[12] | Ding LL (丁玲玲), Qi B (祁彪), Shang ZH (尚占环), Long RJ (龙瑞军), Zhou QX (周启星) (2007). The characteristics of soil microorganism quantity under different alpine grasslands in eastern Qilian Mountain. Journal of Agro-Environment Science (农业环境科学学报), 26, 2104-2111. (in Chinese with English abstract) |
[13] | Fauci MF, Dick RP (1994). Microbial biomass as an indicator of soil quality: effects of long-term management and recent soil amendments. In: Doran JW, Coleman DC, Bezdicek DF, Stewart BA eds. Defining Soil Quality for a Sustainable Environment. Soil Science Society of America, Minneapolis, USA. 229-234. |
[14] | He R (何容), Wang JS (汪家社), Shi Z (施政), Fang YH (方燕鸿), Xu ZK (徐自坤), Quan W (权伟), Zhang ZX (张增信), Ruan HH (阮宏华) (2009). Variations of soil microbial biomass across four different plant communities along an elevation gradient in Wuyi Mountains, China. Acta Ecologica Sinica (生态学报), 29, 5138-5144. (in Chinese with English abstract) |
[15] | Holtmeier FK (2003). Mountain Timberlines: Ecology, Patchiness, and Dynamics. Kluwer Academic Publishers, Dordrecht, the Netherlands. |
[16] | Imberger KT, Chiu CY (2001). Spatial changes of soil fungal and bacterial biomass from a sub-alpine coniferous forest to grassland in a humid, sub-tropical region. Biology and Fertility of Soils, 33, 105-110. |
[17] | IPCC (Intergovernmental Panel on Climate Change) (2007). Contribution of working group III to the fourth assessment report of the intergovernmental panel on climate change. In: Metz B, Davidson OR, Bosch PR, Dave R, Meyer LA eds. Climate Change in 2007: Mitigation. Cambridge University Press, Cambridge, UK. |
[18] | Jaeger CH, Monson RK, Fisk MC, Schmidt SK (1999). Seasonal partitioning of nitrogen by plants and soil microorganisms in an alpine ecosystem. Ecology, 80, 1883-1891. |
[19] | Koponen HT, Jaakkola T, Keinänen-Toivola MM, Kaipainen S, Tuomainen J, Servomaa K, Martikainen PJ (2006). Microbial communities, biomass, and activities in soils as affected by freeze thaw cycles. Soil Biology & Biochemistry, 38, 1861-1871. |
[20] | Körner C, Paulsen J (2004). A world-wide study of high altitude treeline temperatures. Journal of Biogeography, 31, 713-732. |
[21] | Krajick K (2006). Living the high life: the mountaintop environment of the Andes harbors a Noah’s ark of previously undocumented species. Natural History, 115, 44-50. |
[22] | Kupfer JA, Cairns DM (1996). The suitability of montane ecotones as indicators of global climatic change. Progress in Physical Geography, 20, 253-272. |
[23] |
Lipson DA, Monson RK (1998). Plant-microbe competition for soil amino acids in the alpine tundra: effects of freeze- thaw and dry-rewet events. Oecologia, 113, 406-414.
DOI URL PMID |
[24] |
Lipson DA, Schadt CW, Schmidt SK (2002). Changes in soil microbial community structure and function in an alpine dry meadow following spring snow melt. Microbial Ecology, 43, 307-314.
URL PMID |
[25] | Liu MQ (刘满强), Hu F (胡锋), He YQ (何园球), Li HX (李辉信) (2003). Seasonal dynamics of soil microbial biomass and its significance to indicate soil quality under different vegetations restored on degraded red soils. Acta Pedologica Sinica (土壤学报), 40, 937-943. (in Chinese with English abstract) |
[26] | Liu Y (刘洋), Zhang J (张健), Yang WQ (杨万勤) (2009). Responses of alpine biodiversity to climate change. Biodiversity Science (生物多样性), 17, 88-96. (in Chinese with English abstract) |
[27] | Microbial Room of Nanjing Institute of Soil Science, Chinese Academy of Sciences (中国科学院南京土壤研究所微生物室) (1985). Method of Soil Microbial Research (土壤微生物研究法). Science Press, Beijing. (in Chinese) |
[28] | Miller AE, Schimel JP, Sickman JO, Skeen K, Meixner T, Melack JM (2009). Seasonal variation in nitrogen uptake and turnover in two high-elevation soils: mineralization responses are site-dependent. Biogeochemistry, 93, 253-270. |
[29] | Monson RK, Lipson DL, Burns SP, Turnipseed AA, Delany AC, Williams MW, Schmidt SK (2006). Winter forest soil respiration controlled by climate and microbial community composition. Nature, 439, 711-714. |
[30] | Nielsen PL, Andresen LC, Michelsen A, Schmid IK, Kongstad J (2009). Seasonal variations and effects of nutrient applications on N and P and microbial biomass under two temperate heathland plants. Applied Soil Ecology, 42, 279-287. |
[31] |
Pauli H, Gottfried M, Reiter K, Klettner C, Grabherr G (2007). Signals of range expansions and contractions of vascular plants in the high Alps: observations (1994-2004) at the GLORIA master site Schrankogel, Tyrol, Austria. Global Change Biology, 13, 147-156.
DOI URL |
[32] |
Pennisi E (2003). Neither cold nor snow stops tundra fungi. Science, 301, 1307.
DOI URL PMID |
[33] | Qi ZM (齐泽民), Wang KY (王开运) (2009). Soil properties of the subalpine timberline ecotone in Western Sichuan. Journal of Soil and Water Conservation (水土保持学报), 23, 158-162. (in Chinese with English abstract) |
[34] | Qi ZM (齐泽民), Wang KY (王开运), Zhang YB (张远彬), Xie YH (谢玉华) (2009). The soil properties of the subalpine timberline ecotone and adjacent vegetations in Western Sichuan. Acta Ecologica Sinica (生态学报), 29, 6325-6332. (in Chinese with English abstract) |
[35] | Rajaniemi TK, Allison VJ (2009). Abiotic conditions and plant cover differentially affect microbial biomass and community composition on dune gradients. Soil Biology & Biochemistry, 41, 102-109. |
[36] | Risser PG (1995). The status of the science examining ecotones. BioScience, 45, 318-325. |
[37] |
Schadt CW, Martin AP, Lipson DA, Schmidt SK (2003). Seasonal dynamics of previously unknown fungal lineages in tundra soils. Science, 301, 1359-1361.
URL PMID |
[38] |
Schmidt SK, Costello EK, Nemergut DR, Cleveland CC, Reed SC, Weintraub MN, Meyer AF, Martin AP (2007). Biogeochemical consequences of rapid microbial turnover and seasonal succession in soil. Ecology, 88, 1379-1385.
URL PMID |
[39] | Shao BL (邵宝林), Gong GS (龚国淑), Zhang SR (张世熔), Yu X (余霞), Yang DL (杨丹玲), Zhang H (张洪) (2006). Soil microbial quantity and its relations with ecological factors in northern alp region of Hengduan Mountains. Chinese Journal of Ecology (生态学杂志), 25, 885-890. (in Chinese with English abstract) |
[40] | Sun G, Luo P, Wu N, Qiu PF, Gao YH, Chen H, Shi FS (2009). Stellera chamaejasme L. increases soil N availability, turnover rates and microbial biomass in an alpine meadow ecosystem on the eastern Tibetan Plateau of China. Soil Biology & Biochemistry, 41, 86-91. |
[41] |
Waldrop MP, Firestone MK (2006). Seasonal dynamics of microbial community composition and function in oak canopy and open grassland soils. Microbial Ecology, 52, 470-479.
DOI URL PMID |
[42] | Walther GR, Beißner S, Pott R (2005). Climate change and high mountain vegetation shifts. In: Broll G, Keplin B eds. Mountain Ecosystems, Studies in Treeline Ecology. Springer, Berlin. 77-95. |
[43] |
Walther GR, Post E, Convey P, Menzel A, Parmesan C, Beebee TJC, Fromentin JM, Guldberg OH, Bairlein F (2002). Ecological responses to recent climate change. Nature, 416, 389-395.
DOI URL PMID |
[44] | West AW (1986). Improvement of the selective respiratory inhibition technique to measure eukaryote: prokaryote ratios in soils. Journal of Microbiological Methods, 5, 125-138. |
[45] | Wu JS (吴金水) (2006). Soil Microbial Biomass Measurement Method and Its Application (土壤微生物生物量测定方法及其应用). China Meteorological Press, Beijing. (in Chinese) |
[46] | Wu FZ, Yang WQ, Zhang J, Deng RJ (2010). Litter decomposition in two subalpine forests during the freeze-thaw season. Acta Oecologica, 36, 135-140. |
[47] | Yang WQ (杨万勤), Zhang J (张健), Hu TX (胡庭兴) (2006). Forest Soil Ecology (森林土壤生态学). Sichuan Science and Technology Press, Chengdu. (in Chinese) |
[48] | Zhou XQ (周晓庆), Wu FZ (吴福忠), Yang WQ (杨万勤), Zhu JX (朱剑霄) (2011). Dynamics of microbial biomass during litter decomposition in the alpine forest. Acta Ecologica Sinica (生态学报), 31, 4144-4152. (in Chinese with English abstract) |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2022 Chinese Journal of Plant Ecology
Tel: 010-62836134, 62836138, E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn