INFLUENCE OF He-Ne LASER IRRADIATION ON PROTECTIVE ENZYME ACTIVITIES AND LIPID PEROXIDATION IN WHEAT SEEDLINGS BY DROUGHT STRESS DAMAGE

doi:10.3773/j.issn.1005-264x.2008.05.004

Abstract

Abstract:

Aims We studied protective enzyme activities and lipid peroxidation in wheat seedlings treated with different concentration of PEG6000 to determine the effects of He-Ne laser on wheat (Triticum aestivum)exposed to drought stress.

Method We treated 12-day-old, drought-stressed seedlings with 5%, 10% and 15% PEG6000 for 3, 6, 9, 12 and 15 days. After 3, 6, 9, 12 and 15 days of drought stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0, 1 and 3 min. Leaves were sampled for various analyses.

Important findings He-Ne laser irradiation significantly decreased malondialdehyde (MDA) content and superoxide radical (O₂^-.) production rate and notably increased peroxidase (POD) enzyme activity and ascorbate (AsA) and glutathione (GSH) concentration in wheat seedlings exposed to moderate drought stress. We concluded that 3 min laser irradiation enhanced anti-drought characteristics of wheat seedlings treated with 5% or 10% PEG6000.

Key words: He-Ne laser, wheat, drought stress, protective enzyme, lipid peroxidation

QIU Zong-Bo, LIU Xiao, LI Fang-Min, TIAN Xiang-Jun, YUE Ming. INFLUENCE OF He-Ne LASER IRRADIATION ON PROTECTIVE ENZYME ACTIVITIES AND LIPID PEROXIDATION IN WHEAT SEEDLINGS BY DROUGHT STRESS DAMAGE[J]. Chin J Plant Ecol, 2008, 32(5): 1002-1006.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2008.05.004

https://www.plant-ecology.com/EN/Y2008/V32/I5/1002

Figures/Tables 5

References 9

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PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15
0	0	22.58±1.62	27.10±2.05	30.13±3.62	34.51±3.62	32.68±3.06
5	0	31.61±2.85^a	49.70±2.67^a	52.69±17.10^a	54.76±4.52^a	54.19±9.64^a
5	1	30.11±2.61^a	49.68±4.57^a	52.73±13.79^a	53.63±2.67^a	53.38±2.67^a
5	3	28.09±2.65^a	48.71±3.89^a	51.18±10.43^a	53.19±7.82^a	51.29±4.52^a
10	0	34.62±11.37^a	52.69±9.40^a	52.72±11.37^a	56.73±2.67^a	62.76±5.21^a
10	1	30.60±2.61^a	49.68±4.51^a	48.17±2.61^a	55.27±5.25^a	58.71±4.51^a
10	3	23.08±2.61^b	44.19±5.64^b	42.15±2.67^b	44.73±2.67^b	49.14±2.67^b
15	0	35.59±2.67^a	54.63±2.67^a	54.65±4.51^a	58.17±2.67^a	65.23±7.82^a
15	1	34.59±6.89^a	55.16±6.98^a	54.68±7.82^a	56.67±2.67^a	66.20±9.41^a
15	3	37.09±8.72^a	54.70±6.89^a	57.66±6.89^a	59.68±4.57^a	67.22±18.25^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15
0	0	22.58±1.62	27.10±2.05	30.13±3.62	34.51±3.62	32.68±3.06
5	0	31.61±2.85^a	49.70±2.67^a	52.69±17.10^a	54.76±4.52^a	54.19±9.64^a
5	1	30.11±2.61^a	49.68±4.57^a	52.73±13.79^a	53.63±2.67^a	53.38±2.67^a
5	3	28.09±2.65^a	48.71±3.89^a	51.18±10.43^a	53.19±7.82^a	51.29±4.52^a
10	0	34.62±11.37^a	52.69±9.40^a	52.72±11.37^a	56.73±2.67^a	62.76±5.21^a
10	1	30.60±2.61^a	49.68±4.51^a	48.17±2.61^a	55.27±5.25^a	58.71±4.51^a
10	3	23.08±2.61^b	44.19±5.64^b	42.15±2.67^b	44.73±2.67^b	49.14±2.67^b
15	0	35.59±2.67^a	54.63±2.67^a	54.65±4.51^a	58.17±2.67^a	65.23±7.82^a
15	1	34.59±6.89^a	55.16±6.98^a	54.68±7.82^a	56.67±2.67^a	66.20±9.41^a
15	3	37.09±8.72^a	54.70±6.89^a	57.66±6.89^a	59.68±4.57^a	67.22±18.25^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	12d	15d
0	0	1.61±0.62	2.44±0.35	3.12±0.38	3.83±0.28	3.52±0.26
5	0	3.22±0.54^a	3.08±0.23^a	4.50±0.10^a	4.68±0.09^a	4.06±0.80^a
5	1	2.85±1.08^a	2.89±0.06^a	4.26±0.50^a	4.41±0.30^a	3.69±0.52^a
5	3	1.04±0.13^b	2.61±0.31^b	2.64±0.60^b	3.65±0.80^b	3.02±0.14^b
10	0	3.45±0.27^a	3.48±0.09^a	4.83±0.30^a	5.05±0.40^a	4.85±0.40^a
10	1	3.27±0.95^a	3.23±0.09^a	4.28±0.15^a	4.89±0.08^a	4.61±0.58^a
10	3	3.10±0.59^a	3.11±0.04^a	4.22±0.41^a	4.72±0.48^a	4.49±0.04^a
15	0	3.48±1.49^a	4.21±0.21^a	4.68±0.53^a	5.62±0.34^a	5.55±0.48^a
15	1	3.13±0.54^a	4.06±0.56^a	4.86±0.10^a	5.69±0.28^a	5.60±0.52^a
15	3	3.29±0.82^a	4.05±0.06^a	4.71±0.72^a	5.49±0.62^a	5.49±0.82^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	12d	15d
0	0	1.61±0.62	2.44±0.35	3.12±0.38	3.83±0.28	3.52±0.26
5	0	3.22±0.54^a	3.08±0.23^a	4.50±0.10^a	4.68±0.09^a	4.06±0.80^a
5	1	2.85±1.08^a	2.89±0.06^a	4.26±0.50^a	4.41±0.30^a	3.69±0.52^a
5	3	1.04±0.13^b	2.61±0.31^b	2.64±0.60^b	3.65±0.80^b	3.02±0.14^b
10	0	3.45±0.27^a	3.48±0.09^a	4.83±0.30^a	5.05±0.40^a	4.85±0.40^a
10	1	3.27±0.95^a	3.23±0.09^a	4.28±0.15^a	4.89±0.08^a	4.61±0.58^a
10	3	3.10±0.59^a	3.11±0.04^a	4.22±0.41^a	4.72±0.48^a	4.49±0.04^a
15	0	3.48±1.49^a	4.21±0.21^a	4.68±0.53^a	5.62±0.34^a	5.55±0.48^a
15	1	3.13±0.54^a	4.06±0.56^a	4.86±0.10^a	5.69±0.28^a	5.60±0.52^a
15	3	3.29±0.82^a	4.05±0.06^a	4.71±0.72^a	5.49±0.62^a	5.49±0.82^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	9d	12d	15d
0	0	8.40±0.85	9.42±0.65	10.21±1.28	10.43±2.65	11.27±2.36
5	0	8.53±0.92^b	8.13±0.58^b	10.47±1.61^b	11.20±1.72^b	15.13±1.31^b
5	1	8.93±1.36^b	8.60±0.53^b	10.97±1.49^b	12.47±2.39^b	16.20±1.89^a
5	3	10.93±1.64^a	12.60±1.72^a	13.23±1.44^a	14.33±1.98^a	17.53±1.77^a
10	0	10.30±1.90^a	11.47±1.61^a	13.57±1.63^a	14.47±1.12^a	24.87±1.66^a
10	1	12.40±1.60^a	11.40±1.59^a	13.80±1.58^a	16.20±1.87^a	26.00±1.58^a
10	3	10.53±1.64^a	11.53±1.57^a	13.07±1.15^a	15.40±1.72^a	25.07±1.67^a
15	0	12.70±1.46^a	12.10±1.75^a	12.73±1.64^a	12.40±1.02^a	18.00±2.27^a
15	1	12.00±1.87^a	12.60±2.86^a	13.73±0.95^a	13.27±2.53^a	18.67±1.45^a
15	3	12.27±1.60^a	12.80±1.58^a	12.80±1.20^a	13.87±1.92^a	18.53±1.84^a