EFFECTS OF ARBUSCULAR MYCORRHIZAL FUNGAL INOCULATION ON GROWTH AND PHOTOSYNjournal OF CAMPTOTHECA ACUMINATA SEEDLINGS

doi:10.3773/j.issn.1005-264x.2009.04.017

Abstract

Abstract:

Aims Camptotheca acuminata is a Chinese tree that produces camptothecin (CPT), a monoterpenoid indole alkaloid that inhibits tumor cells. Our purpose was to determine effects of arbuscular mycorrhizal (AM) fungi on the growth and photosynjournal of C. acuminata seedlings.
Methods Potted seedlings were inoculated with one of six AM fungi (Glomus manihot, G. versiforme, G. diaphanum, Acaulospora mellea, A. laevis and Sclerocystis sinuosa) and grown under greenhouse conditions.
Important findings All seedling roots were colonized by the six AM fungi and formed typical mycorrhizal structures. Except the G. versiforme inoculated seedlings, the biomass of mycorrhizal seedlings was significantly higher than non-mycorrhizal (CK) seedlings. Moreover, biomass of A. mellea, S. sinuosa and G. diaphanum inoculated seedlings reached 1.6, 1.4 and 1.3 times that of CK, respectively. Compared with non-mycorrhizal seedlings, the net photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) of A. mellea inoculated seedlings were significantly increased, while the intercellular CO₂concentration (C_i) and stomatal limitation value (L_s) of mycorrhizal seedling leaves did not change significantly. The chlorophyll a content, total chlorophyll content, chlorophyll a/b value and carotenoid content of G. diaphanum, A. mellea, A. laevis and S. sinuosa inoculated seedlings were significantly higher than that of CK, as was the chlorophyll b content of G. manihot and S. sinuosa inoculated seedlings. Effects of AM fungal inoculation on the parameters of chlorophyll fluorescence of Camptotheca acuminata seedlings were small. Only the maximal photochemical efficiency of PSII in the dark (F_v/F_m) of G. diaphanum inoculated seedlings was significantly higher than that of CK. For G. manihot and S. sinuosa inoculated seedlings, the effective quantum yield (EQR) was higher than that of the non-mycorrhizal seedlings. The photochemical quenching (qP) of S. sinuosa inoculated seedlings was higher and the non-photochemical quenching (NPQ) was lower than in non-mycorrhizal seedlings.

Key words: Camptotheca acuminata, arbuscular mycorrhiza (AM), chlorophyll, photosynjournal, chlorophyll fluorescence

ZHAO Xin, SONG Rui-Qing, YAN Xiu-Feng. EFFECTS OF ARBUSCULAR MYCORRHIZAL FUNGAL INOCULATION ON GROWTH AND PHOTOSYNjournal OF CAMPTOTHECA ACUMINATA SEEDLINGS[J]. Chin J Plant Ecol, 2009, 33(4): 783-790.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.04.017

https://www.plant-ecology.com/EN/Y2009/V33/I4/783

Figures/Tables 5

References 31

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处理 Treatments	株高 Height (cm)	地径 Diameter of stem (cm)	根冠比 Root/shoot radio	生物量 Biomass (g)
CK	18.78±1.52^ab	1.77±0.08^a	0.62±0.12^a	3.12±0.32^a
Glomus manihot	18.28±0.81^a	1.77±0.15^a	0.79±0.10^b	3.59±0.22^b
Glomus versiforme	18.71±0.58^ab	1.77±0.04^a	0.90±0.13^c	3.49±0.20^ab
Glomus diaphanum	20.61±0.24^b	1.95±0.08^b	0.62±0.07^a	4.06±0.24^c
Acaulospora mellea	23.25±1.51^c	1.99±0.09^b	0.73±0.08^ab	4.92±0.64^d
Acaulospora laevis	19.43±1.32^ab	1.78±0.09^a	0.83±0.09^bc	3.56±0.36^b
Sclerocystis sinuosa	22.58±1.10 ^c	1.85±0.03^ab	0.67±0.09 ^a	4.40±0.34 ^c

处理 Treatments	株高 Height (cm)	地径 Diameter of stem (cm)	根冠比 Root/shoot radio	生物量 Biomass (g)
CK	18.78±1.52^ab	1.77±0.08^a	0.62±0.12^a	3.12±0.32^a
Glomus manihot	18.28±0.81^a	1.77±0.15^a	0.79±0.10^b	3.59±0.22^b
Glomus versiforme	18.71±0.58^ab	1.77±0.04^a	0.90±0.13^c	3.49±0.20^ab
Glomus diaphanum	20.61±0.24^b	1.95±0.08^b	0.62±0.07^a	4.06±0.24^c
Acaulospora mellea	23.25±1.51^c	1.99±0.09^b	0.73±0.08^ab	4.92±0.64^d
Acaulospora laevis	19.43±1.32^ab	1.78±0.09^a	0.83±0.09^bc	3.56±0.36^b
Sclerocystis sinuosa	22.58±1.10 ^c	1.85±0.03^ab	0.67±0.09 ^a	4.40±0.34 ^c

处理 Treatment	净光合速率 Net photosynthetic rate (P_n) (μmol·m^-2·s^-1)	气孔导度 Stomatal conductance (G_s) (μmol·m^-2·s^-1)	蒸腾速率 Transpiration rate (T_r) (mmol·m^-2·s^-1 )	胞间CO₂浓度 Intercellular CO₂concentration (C_i) (μmol CO₂·m^-2·s^-1)	气孔限制值 Stomatal limitation value (L_s)
CK	5.03±0.51^ab	22.33±5.77^a	0.97±0.14^a	253.00±14.00^a	0.28±0.04^a
Glomus manihot	4.93±0.64^a	21.00±5.00^a	0.97±0.12^a	288.00±10.54^b	0.18±0.03^b
Glomus versiforme	5.37±0.58^b	47.67±9.23^bc	1.45±0.27^bc	239.30±22.85^a	0.32±0.07^a
Glomus diaphanum	5.90±0.61^bc	31.67±12.58^ab	1.11±0.14^ab	251.00±20.66^a	0.28±0.06^a
Acaulospora mellea	6.40±0.53^c	52.33±14.84^c	1.54±0.40^c	234.30±11.85^a	0.33±0.03^a
Acaulospora laevis	5.73±0.50^bc	34.33±4.62^b	1.20±0.24^b	248.70±20.03^a	0.29±0.06^a
Sclerocystis sinuosa	6.00±0.53^bc	35.00±6.93^b	1.26±0.17^b	261.70±2.52^ab	0.25±0.01^ab

处理 Treatment	净光合速率 Net photosynthetic rate (P_n) (μmol·m^-2·s^-1)	气孔导度 Stomatal conductance (G_s) (μmol·m^-2·s^-1)	蒸腾速率 Transpiration rate (T_r) (mmol·m^-2·s^-1 )	胞间CO₂浓度 Intercellular CO₂concentration (C_i) (μmol CO₂·m^-2·s^-1)	气孔限制值 Stomatal limitation value (L_s)
CK	5.03±0.51^ab	22.33±5.77^a	0.97±0.14^a	253.00±14.00^a	0.28±0.04^a
Glomus manihot	4.93±0.64^a	21.00±5.00^a	0.97±0.12^a	288.00±10.54^b	0.18±0.03^b
Glomus versiforme	5.37±0.58^b	47.67±9.23^bc	1.45±0.27^bc	239.30±22.85^a	0.32±0.07^a
Glomus diaphanum	5.90±0.61^bc	31.67±12.58^ab	1.11±0.14^ab	251.00±20.66^a	0.28±0.06^a
Acaulospora mellea	6.40±0.53^c	52.33±14.84^c	1.54±0.40^c	234.30±11.85^a	0.33±0.03^a
Acaulospora laevis	5.73±0.50^bc	34.33±4.62^b	1.20±0.24^b	248.70±20.03^a	0.29±0.06^a
Sclerocystis sinuosa	6.00±0.53^bc	35.00±6.93^b	1.26±0.17^b	261.70±2.52^ab	0.25±0.01^ab

处理 Treatment	叶绿素a含量 Chlorophyll a content (mg·g^-1)	叶绿素b含量 Chlorophyll b content (mg·g^-1)	叶绿素a/b Chlorophyll a/b value	总叶绿素含量 Total chlorophyll content (mg·g^-1)	类胡萝卜素含量 Carotenoid content (mg·g^-1)
CK	2.140±0.075^a	0.750±0.007^a	2.855±0.075^a	2.890±0.082^a	0.331±0.022^a
Glomus manihot	2.367±0.016^b	0.854±0.044^b	2.777±0.140^a	3.221±0.052^b	0.393±0.005^bd
Glomus versiforme	1.881±0.108^c	0.601±0.076^c	3.145±0.212^b	2.482±0.184^c	0.283±0.030^c
Glomus diaphanum	2.443±0.056^bd	0.752±0.042^a	3.253±0.185^bc	3.196±0.076^b	0.354±0.046^b
Acaulospora mellea	2.727±0.064^d	0.770±0.029^a	3.542±0.055^c	3.497±0.092^d	0.392±0.025^bd
Acaulospora laevis	2.514±0.064^bd	0.773±0.024^a	3.254±0.169^bc	3.287±0.051^b	0.388±0.014^bd
Sclerocystis sinuosa	2.858±0.046^d	0.857±0.029^b	3.340±0.168^bc	3.715±0.023^d	0.419±0.047^d