Analysis of main volatile organic compounds and study of aboveground structures in Artemisia frigida

doi:10.3773/j.issn.1005-264x.2010.04.012

Abstract

Abstract:

Aims Special volatile organic compounds (VOCs) are released from Artemisia frigida in its growth, and the smell of VOCs is stronger when it is damaged. Our objective was to determine the VOCs from A. frigida and the relationship between the VOCs release and organization structure of A. frigida.

Methods VOCs were collected using the dynamic headspace air-circulation method and analyzed with the thermal-desorption cold trap/gas chromatography/mass spectrum (TCT/GC/MS). The coat hairs of A. frigida were observed with scanning electron microscope, and the paraffin section of aboveground structures was observed with microscope.

Important findings Twenty-two main compounds were identified in undamaged A. frigida: camphene (14.27%), (E)-3-hexen-1-ol,acetate (10.85%), p-cymene (9.05%), eucalyptol (39.80%), α-terpineol (10.04%), β-terpineol (2.48%), camphor (5.66%) and (R)-(-)-p-menth-1-en-4-ol (3.84%). Twelve kinds of VOCs increased with damage; those with a relative content > 1% were cis-3-Hexenal (1.15%), 2-Pentenal (1.34%), cis-Geraniol (2.66%), Borneol (4.47%), (1R,4R)-(+)-p-Mentha-2,8-diene (9.15%), Bornyl acetate (1.37%) and Eudesma-4(14),11-diene (1.30%). There were many palisade tissues in A. frigida blades, 2-3 palisade tissues in petioles, and many air chambers in palisade tissue connected with stoma. The kinds and relative content of damaged A. frigida VOCs were more than the undamaged, possibly because A. frigida VOCs were stored in air chambers, which were released when A. frigida was damaged, and some new VOCs were composed and released. Therefore, the kinds and content of VOCs were increased in damaged A. frigida.

Key words: aboveground structure, Artemisia frigida, component analysis, volatile organic compounds

ZUO Zhao-Jiang, ZHANG Ru-Min, WANG Yong, HOU Ping, WEN Guo-Sheng, GAO Yan. Analysis of main volatile organic compounds and study of aboveground structures in Artemisia frigida[J]. Chin J Plant Ecol, 2010, 34(4): 462-468.

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https://www.plant-ecology.com/EN/Y2010/V34/I4/462

Figures/Tables 4

References 26

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保留时间 Retention time (min)		挥发性有机化合物 Volatile organic compounds		分子式 Chemical formula	相对含量 Relative content (%)
未损伤 UDa	损伤Da	中文名称 Chinese name	英文名称 English name	分子式 Chemical formula	未损伤 UDa	损伤 Da
	13.51	顺-3-己烯醛	cis-3-Hexenal	C₆H₁₀O	-	1.15 ± 0.13
	15.57	2-己烯醛	2-Hentenal	C₆H₁₀O	-	1.34 ± 0.18
15.57	15.82	3-己烯醇	3-Hexenol(c,t)	C₆H₁₂O	0.05 ± 0.01	2.81 ± 0.23
	16.14	己醇	1-Hexanol	C₆H₁₄O	-	0.50 ± 0.04
	16.23	乙酸异戊酯	Isopentyl alcohol,acetate	C₇H₁₄O₂	-	0.17 ± 0.05
18.01	18.02	α-蒎烯	α-Pinene	C₁₀H₁₆	0.03 ± 0.01	0.04 ± 0.00
18.38	18.40	β-蒎烯	β-Pinene	C₁₀H₁₆	0.06 ± 0.01	0.05 ± 0.01
19.05	19.06	檀香三烯	Santolina triene	C₁₀H₁₆	0.06 ± 0.01	0.07 ± 0.01
19.71	19.71	水芹烯	Phellandrene	C₁₀H₁₆	0.15 ± 0.02	0.39 ± 0.09
20.07	20.09	莰烯	Camphene	C₁₀H₁₆	14.27 ± 1.29	6.28 ± 0.58
	20.29	1-壬烯-3-醇	1-Nonen-3-ol	C₉H₁₈O	-	0.58 ± 0.07
20.56	20.74	(E)-乙酸-3-己烯酯	(E)-3-Hexen-1-ol,acetate	C₈H₁₄O₂	10.85 ± 0.83	10.96 ± 1.37
21.54	21.79	对-伞花烃	p-Cymene	C₁₀H₁₄	9.05 ± 0.79	4.11 ± 0.08
21.95	22.02	桉树脑	Eucalyptol	C₁₀H₁₈O	39.80 ± 2.24	22.43 ± 2.49
22.20		2,4-癸二烯-1-醇	2,4-Decadien-1-ol	C₁₀H₁₈O	0.15 ± 0.03	-
22.47	22.55	萜品烯	Terpinen	C₁₀H₁₆	0.93 ± 0.11	1.12 ± 0.27
23.08	23.27	α-萜品醇	α-Terpineol	C₁₀H₁₈O	10.04 ± 0.98	11.01 ± 0.99
	23.75	里纳醇	Linalol	C₁₀H₁₈O	-	0.13 ± 0.07
23.90	23.94	黄瓜醇	Cucumber alcohol	C₉H₁₆O	0.07 ± 0.01	0.23 ± 0.04
24.07	24.22	β-萜品醇	β-Terpineol	C₁₀H₁₈O	2.48 ± 0.45	3.56 ± 0.26
25.67	25.76	樟脑	Camphor	C₁₀H₁₈O	5.66 ± 0.97	7.85 ± 0.84
	25.87	顺-牻牛儿醇	cis-Geraniol	C₁₀H₁₈O	-	2.66 ± 0.47
26.45		(R)-(-)-对-薄荷-1-烯-4-醇	(R)-(-)-p-Menth-1-en-4-ol	C₁₀H₁₈O	3.84 ± 0.74	-
	26.58	冰片	Borneol	C₁₀H₁₈O	-	4.47 ± 0.33
26.82	26.94	(S)-(-)-对-薄荷-1-烯-8-醇	(S)-(-)-p-menth-1-en-8-ol	C₁₀H₁₈O	0.88 ± 0.12	1.80 ± 0.18
28.64		乙酸橙花酯	Nerol acetate	C₁₂H₂₀O₂	1.00 ± 0.21	-
	28.81	(1R,4R)-(+)-对-薄荷2,8-二烯	(1R,4R)-(+)-p-Mentha-2,8-diene	C₁₂H₂₀	-	9.15 ± 0.86
	29.27	乙酸冰片酯	Bornyl acetate	C₁₂H₂₀O₂	-	1.37 ± 0.29
31.71	31.75	古巴烯	Copaene	C₁₅H₂₄	0.25 ± 0.04	1.50 ± 0.15
32.98	33.02	(Z)-法呢烯	(Z)-Farnesene	C₁₅H₂₄	0.02 ± 0.00	0.13 ± 0.02
	34.32	柏木烯	Cedrene	C₁₅H₂₄	-	0.33 ± 0.03
34.42	34.50	大根叶香烯D	Germacrene D	C₁₅H₂₄	0.12 ± 0.01	2.48 ± 0.14
34.70		异石竹烯	Isocaryophillene	C₁₅H₂₄	0.21 ± 0.01	-
	34.76	4(14), 11-桉叶双烯	Eudesma-4(14),11-diene	C₁₅H₂₄	-	1.30 ± 0.13

保留时间 Retention time (min)		挥发性有机化合物 Volatile organic compounds		分子式 Chemical formula	相对含量 Relative content (%)
未损伤 UDa	损伤Da	中文名称 Chinese name	英文名称 English name	分子式 Chemical formula	未损伤 UDa	损伤 Da
	13.51	顺-3-己烯醛	cis-3-Hexenal	C₆H₁₀O	-	1.15 ± 0.13
	15.57	2-己烯醛	2-Hentenal	C₆H₁₀O	-	1.34 ± 0.18
15.57	15.82	3-己烯醇	3-Hexenol(c,t)	C₆H₁₂O	0.05 ± 0.01	2.81 ± 0.23
	16.14	己醇	1-Hexanol	C₆H₁₄O	-	0.50 ± 0.04
	16.23	乙酸异戊酯	Isopentyl alcohol,acetate	C₇H₁₄O₂	-	0.17 ± 0.05
18.01	18.02	α-蒎烯	α-Pinene	C₁₀H₁₆	0.03 ± 0.01	0.04 ± 0.00
18.38	18.40	β-蒎烯	β-Pinene	C₁₀H₁₆	0.06 ± 0.01	0.05 ± 0.01
19.05	19.06	檀香三烯	Santolina triene	C₁₀H₁₆	0.06 ± 0.01	0.07 ± 0.01
19.71	19.71	水芹烯	Phellandrene	C₁₀H₁₆	0.15 ± 0.02	0.39 ± 0.09
20.07	20.09	莰烯	Camphene	C₁₀H₁₆	14.27 ± 1.29	6.28 ± 0.58
	20.29	1-壬烯-3-醇	1-Nonen-3-ol	C₉H₁₈O	-	0.58 ± 0.07
20.56	20.74	(E)-乙酸-3-己烯酯	(E)-3-Hexen-1-ol,acetate	C₈H₁₄O₂	10.85 ± 0.83	10.96 ± 1.37
21.54	21.79	对-伞花烃	p-Cymene	C₁₀H₁₄	9.05 ± 0.79	4.11 ± 0.08
21.95	22.02	桉树脑	Eucalyptol	C₁₀H₁₈O	39.80 ± 2.24	22.43 ± 2.49
22.20		2,4-癸二烯-1-醇	2,4-Decadien-1-ol	C₁₀H₁₈O	0.15 ± 0.03	-
22.47	22.55	萜品烯	Terpinen	C₁₀H₁₆	0.93 ± 0.11	1.12 ± 0.27
23.08	23.27	α-萜品醇	α-Terpineol	C₁₀H₁₈O	10.04 ± 0.98	11.01 ± 0.99
	23.75	里纳醇	Linalol	C₁₀H₁₈O	-	0.13 ± 0.07
23.90	23.94	黄瓜醇	Cucumber alcohol	C₉H₁₆O	0.07 ± 0.01	0.23 ± 0.04
24.07	24.22	β-萜品醇	β-Terpineol	C₁₀H₁₈O	2.48 ± 0.45	3.56 ± 0.26
25.67	25.76	樟脑	Camphor	C₁₀H₁₈O	5.66 ± 0.97	7.85 ± 0.84
	25.87	顺-牻牛儿醇	cis-Geraniol	C₁₀H₁₈O	-	2.66 ± 0.47
26.45		(R)-(-)-对-薄荷-1-烯-4-醇	(R)-(-)-p-Menth-1-en-4-ol	C₁₀H₁₈O	3.84 ± 0.74	-
	26.58	冰片	Borneol	C₁₀H₁₈O	-	4.47 ± 0.33
26.82	26.94	(S)-(-)-对-薄荷-1-烯-8-醇	(S)-(-)-p-menth-1-en-8-ol	C₁₀H₁₈O	0.88 ± 0.12	1.80 ± 0.18
28.64		乙酸橙花酯	Nerol acetate	C₁₂H₂₀O₂	1.00 ± 0.21	-
	28.81	(1R,4R)-(+)-对-薄荷2,8-二烯	(1R,4R)-(+)-p-Mentha-2,8-diene	C₁₂H₂₀	-	9.15 ± 0.86
	29.27	乙酸冰片酯	Bornyl acetate	C₁₂H₂₀O₂	-	1.37 ± 0.29
31.71	31.75	古巴烯	Copaene	C₁₅H₂₄	0.25 ± 0.04	1.50 ± 0.15
32.98	33.02	(Z)-法呢烯	(Z)-Farnesene	C₁₅H₂₄	0.02 ± 0.00	0.13 ± 0.02
	34.32	柏木烯	Cedrene	C₁₅H₂₄	-	0.33 ± 0.03
34.42	34.50	大根叶香烯D	Germacrene D	C₁₅H₂₄	0.12 ± 0.01	2.48 ± 0.14
34.70		异石竹烯	Isocaryophillene	C₁₅H₂₄	0.21 ± 0.01	-
	34.76	4(14), 11-桉叶双烯	Eudesma-4(14),11-diene	C₁₅H₂₄	-	1.30 ± 0.13