EFFECTS OF ENVIRONMENTAL STRESSES ON CELASTROL CONTENTS IN TRIPTERYGIUM WILFORDII

doi:10.3773/j.issn.1005-264x.2009.01.020

Abstract

Abstract:

Aims Tripterygium wilfordii is a highly valuable traditional Chinese medicinal plant. Celastrol, an active compound of T. wilfordii, has recently received attention from pharmaceutical researchers due to its medicinal value. Environmental stress may promote the formation and accumulation of secondary metabolites of medicinal plants. Our objective was to investigate whether mild environmental stress increased the content of Celastrol in T. wilfordii.
Methods Orthogonal comparison of mild stress treatments of water, sunlight and N content were performed on the seedlings of T. wilfordii in the greenhouse. The content of Celastrol was determined by High Performance Liquid Chromatography (HPLC).
Important findings Celastrol was mainly produced in the seedling root and was increased 10.8%, 14.4% and 13.0% under the three different stress treatments, respectively. Therefore, mild stress treatment improves the synthesis and accumulation of Celastrol.

Key words: Tripterygium wilfordii, environmental stress, Celastrol, secondary metabolism

DU Wei-Wei, YAO Xiao-Hong, HUANG Hong-Wen. EFFECTS OF ENVIRONMENTAL STRESSES ON CELASTROL CONTENTS IN TRIPTERYGIUM WILFORDII[J]. Chin J Plant Ecol, 2009, 33(1): 180-185.

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URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.01.020

https://www.plant-ecology.com/EN/Y2009/V33/I1/180

Figures/Tables 6

Fig. 1 Chemical structure of Celastrol (Duan et al., 2000, 2001)

Table 1 The Analysis of Variance (ANOVA) of between-subjects effects on content of Celastrol under different stress treatments

变异来源 Source	平方和 SS	自由度 df	样本均方 Mean square	F值 F	显著性 Sig.
模型 Model	2 116.893^a	8	264.612	2 111.155	0.000
l	9.445	1	9.445	46.304	0.000
r	6.875	1	6.875	33.704	0.000
n	7.746	1	7.746	37.974	0.000
l×r	1.482	1	1.482	11.822	0.002
l×n	0.127	1	0.127	1.014	0.321
r×n	1.475	1	1.475	11.769	0.002
l×r×n	0.248	1	0.248	1.979	0.169
误差 Error	4.011	32	0.125
总数 Total	2 120.904	40

Fig. 2 The content of Celastrol in Tripterygium wilfordii under different stress conditions

Fig. 3 Effects of water stress on Celastrol content in Tripterygium wilfordii

Fig. 4 Effects of light stress on Celastrol content in Tripterygium wilfordii

Fig. 5 Effects of N content stress in soil on the content of Celastrol in Tripterygium wilfordii

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