EFFECTS OF WATERLOGGING STRESS ON MORPHOLOGY AND PHYSIOLOGY OF CAMPTOTHECA ACUMINATA

doi:10.3773/j.issn.1005-264x.2009.01.015

Abstract

Abstract:

Aims Camptotheca acuminate is a common tree species in southern and eastern China. It is planted widely as a virescence species and is cultivated as an officinal species, because its seed, leaf and stem can be used to distill camptothecin, which is used to treat cancer. Our objective was to examine C. acuminate’s resistance to waterlogging.
Methods One-year seedlings of C. acuminata were grown in pots in a greenhouse in January, and we implemented four treatments in May: CK (common soil water content), A (water level 10 cm under soil surface), B (water level equal with soil surface) and C (water level 4 cm above soil surface). Treatments lasted 21 days, and indexes of lenticels, root growth, root vigor, POD, SOD, MDA and LDH were determined at different times after treatment.
Important findings Root growth was better in A and CK treatments, and roots gradually died in B and C treatments. Comparatively, root vigor was higher in A and decreased gradually under B and C with treatment time. Many lenticels were observed on the stem under B and C, but not under CK and A. The POD and SOD activity of leaves of A treatment were higher during treatment, but increased and then decreased under B and C treatments. The production rate of O₂^-·, H₂O₂ content and MDA content of leaves of A, B and C treatments increased gradually with treatment time, but was higher under C and B treatments. The LDH activity of roots in A, B and C treatments was lower early in treatment, but increased significantly late in treatment. We conclude that C. acuminata grows well in lightly waterlogged sites, but not in waterlogged and flooded sites.

Key words: Camptotheca acuminata Decne, waterlogging stress, morphology, physiology

WANG Gui-Bin, CAI Jin-Feng, HE Xiao-Hua. EFFECTS OF WATERLOGGING STRESS ON MORPHOLOGY AND PHYSIOLOGY OF CAMPTOTHECA ACUMINATA[J]. Chin J Plant Ecol, 2009, 33(1): 134-140.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2009.01.015

https://www.plant-ecology.com/EN/Y2009/V33/I1/134

Figures/Tables 4

References 22

[1]	Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T (2002). Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Science, 163, 117-123. DOI URL
[2]	Chen GX, Asada K (1992). Inactivation of ascorbate peroxidase by thiols requires hydrogen peroxide. Plant and Cell Physiology, 33, 117-123.
[3]	Colmer TD (2003). Aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep water rice. Annals of Botany, 91, 301-309. DOI URL PMID
[4]	Crawford LA, Bown AW, Breitkreuz KE, Guinel FC (1994). The synthesis of γ-aminobutyric acid in response to treatments reducing cytosolic pH. Plant Physiology, 104, 865-871. DOI URL PMID
[5]	He ST (何嵩涛), Liu GQ (刘国琴), Fan WG (樊伟国) (2000). Physiological response to flooding stress on ginkgo(Ⅰ)—A study on membrane lipid peroxidation and cell protective enzyme activity effects of ginkgo under flooding stress. Journal of Mountain Agriculture and Biology (山地农业生物学报), 19, 272-275. (in Chinese with English abstract)
[6]	Institute of Shanghai Plant Physiology of Chinese Academy of Sciences (中国科学院上海植物生理研究所) (2004). Experimental Guide of Modern Plant Physiology (现代植物生理学实验指南). Science Press, Beijing. (in Chinese)
[7]	Keles Y, Öncel I (2000). Response of antioxidative defence system to temperature and water stress combinations in wheat seedlings. Plant Science, 163, 783-790. DOI URL
[8]	Ma L (马林) (2007). Callus induction and cultivation of Camptotheca acuminata. China Journal of Chinese Materia Medica (中国中药杂志), 21, 1273-1276. (in Chinese)
[9]	Pan XW (潘学武), Liu X (刘欣), Lü YT (吕应堂) (2005). Optimization of cell suspension cultures in hypocotyls of Camptotheca acuminata and camptothecin accumulation. Chinese Traditional and Herbal Drugs (中草药), 36, 1221-1225. (in Chinese with English abstract)
[10]	Plaxton WC (1996). The organization and regulation of plant glycolysis. Annual Review of Plant Physiology and Plant Molecular Biology, 47, 185-214. DOI URL PMID
[11]	Schaffer B, Andersen PC, Ploetz RC (1992). Responses of fruit crops to flooding. Horticultural Reviews, 13, 257-313.
[12]	Shi JX (石进校), Liu YD (刘应迪), Chen J (陈军), Zhang SF (张淑芳) (2002). Activation of SOD and content of MDA in Epimedium grandiflorm Morr leaves under water logging stress in soil. Life Science Research (生命科学研究), 6, 160-162. (in Chinese with English abstract)
[13]	Tang ZC (汤章城) (2000). Experimental Guide of Modern Plant Physiology (现代植物生理学实验指导). Science Press, Beijing. (in Chinese)
[14]	Thomas AL, Guerreiro SMC, Sodek L (2005). Aerenchyma formation and recovery from hypoxia of the flooded root system of nodulated soybean. Annals of Botany, 97, 1191-1198.
[15]	Visser EJW, Voesenek LACJ, Vartapetian BB, Jackson MB (2003). Flooding and plant growth. Annals of Botany, 91, 107-109. DOI URL
[16]	Wu JS (吴家胜), Ying YQ (应叶青), Zhou GM (周国模), Shi BL (石柏林), Jiang F (江峰), Zhang HJ (张豪杰), Hong ZB (洪祖兵) (2005). The effects of N, P and K fertilizer applied in leaf-producing plantation of Camptotheca acuminata Decne. Journal of Northeast Forestry University (东北林业大学学报), 33(3), 29-31. (in Chinese with English abstract)
[17]	Yan B, Dai Q, Liu X, Huang S, Wang Z (1996). Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant and Soil, 179, 261-268. DOI URL
[18]	Ye Y, TAM Nora FY, Wong YS, Lu CY (2003). Growth and physiological responses of two mangrove species ( Bruguiera gymnorrhiza and Kandelia candel) to waterlogging. Environmental and Experimental Botany, 49, 209-221. DOI URL
[19]	Ying YQ (应叶青), Wu JS (吴家胜), Zhou GM (周国模), Sun WQ (孙伟琴), Chen YQ (陈有全) (2004). Study on variations of seedling-stage trait of Camptotheca acuminata provenances. Forest Research (林业科学研究), 17, 751-756. (in Chinese with English abstract)
[20]	Yordanova RY, Christov KN, Popova LP (2004). Antioxidative enzymes in barley plants subjected to soil flooding. Environmental and Experimental Botany, 51, 93-101. DOI URL
[21]	Zhang YQ (张永清), Miao GY (苗果园) (2005). The biological response of sorghum root system under water logging stress at later growth stages. Journal of Shanxi Agricultural University (Nature Science Edition) (山西农业大学学报(自然科学版)), 3, 193-195. (in Chinese with English abstract)
[22]	Zhou GM (周国模), Li XQ (李孝青), Chu JM (储家淼), Chen ZA (陈祖安), Xu SY (许绍远) (2000). Camptotheca acuminata:the law of growth and character correlation of young trees and sprouting branches. Journal of Zhejiang Forestry College (浙江林学院学报), 17, 355-359. (in Chinese with English abstract)

处理时间 Treatment time (d)	1	2	3	6	13	21
CK	194.9±12.1^a	207.1±15.9^a	199.9±14.1^a	181.8±12.9^a	190.9±15.1^a	193.9±14.9^a
A	185.2±13.2^a	205.8±14.4^a	196.0±13.4^a	166.6±12.1^a	161.7±15.4^a	152.9±14.3^a
B	199.0±14.4^a	174.6±12.8^b	155.2±15.5^b	116.4±11.8^b	77.6±8.4^b	38.8±6.7^b
C	203.2±15.7^a	183.2±13.9^b	142.6±14.1^c	108.9±12.0^c	68.3±7.9^c	19.8±5.5^c

处理时间 Treatment time (d)	1	2	3	6	13	21
CK	194.9±12.1^a	207.1±15.9^a	199.9±14.1^a	181.8±12.9^a	190.9±15.1^a	193.9±14.9^a
A	185.2±13.2^a	205.8±14.4^a	196.0±13.4^a	166.6±12.1^a	161.7±15.4^a	152.9±14.3^a
B	199.0±14.4^a	174.6±12.8^b	155.2±15.5^b	116.4±11.8^b	77.6±8.4^b	38.8±6.7^b
C	203.2±15.7^a	183.2±13.9^b	142.6±14.1^c	108.9±12.0^c	68.3±7.9^c	19.8±5.5^c

处理时间 Treatment time (d)		1	2	3	6	13	21
氧自由基 O₂^-· (nmol·g^-1FW)	CK	27.7±5.2^a	44.1±6.9^a	38.7±5.6^a	45.9±5.6^a	43.6±5.1^a	48.6±7.2^a
	A	28.9±4.6^a	33.9±4.9^a	49.1±6.0^a	47.9±4.2^a	47.4±6.3^a	42.6±5.4^a
	B	38.4±6.5^b	49.4±6.6^b	53.9±6.8^b	56.1±6.8^b	73.4±7.0^b	94.6±8.9^b
	C	36.6±7.7^b	63.2±7.9^c	61.3±7.0^c	71.0±7.5^c	82.5±7.4^c	118.1±9.8^c
过氧化氢含量 H₂O₂ content (μmol·g^-1FW)	CK	332.1±46.9^a	345.1±43.8^a	384.2±38.7^a	400.0±58.6^a	418.3±49.4^a	455.2±58.5^a
	A	301.4±55.4^a	472.1±46.7^b	454.1±52.2^b	521.2±50.8^b	490.9±75.8^b	697.4±68.6^b
	B	414.8±65.4^b	567.9±51.4^c	564.9±61.4^c	653.2±81.6^c	998.4±68.4^c	1040.6±75.8^c
	C	482.9±60.9^c	602.9±75.3^d	683.6±68.4^d	1018.3±77.5^d	1241.0±69.2^d	1244.3±65.3^d
丙二醛含量 MDAcontent (μmol·g^-1FW)	CK	79.6±11.7^a	89.4±10.9^a	87.4±9.7^a	96.2±14.7^a	100.9±12.4^a	124.9±14.6^a
	A	90.6±13.9^b	91.2±11.7^b	98.2±13.1^b	125.7±12.7^b	127.6±19.0^b	138.6±17.1^b
	B	106.9±16.4^c	108.9±12.9^c	120.6±15.3^c	162.9±20.4^c	190.5±17.1^c	202.9±18.9^c
	C	103.7±15.2^d	131.8±18.8^d	147.1±17.1^d	215.9±19.4^d	213.0±17.3^d	244.9±16.3^d

处理时间 Treatment time (d)		1	2	3	6	13	21
氧自由基 O₂^-· (nmol·g^-1FW)	CK	27.7±5.2^a	44.1±6.9^a	38.7±5.6^a	45.9±5.6^a	43.6±5.1^a	48.6±7.2^a
	A	28.9±4.6^a	33.9±4.9^a	49.1±6.0^a	47.9±4.2^a	47.4±6.3^a	42.6±5.4^a
	B	38.4±6.5^b	49.4±6.6^b	53.9±6.8^b	56.1±6.8^b	73.4±7.0^b	94.6±8.9^b
	C	36.6±7.7^b	63.2±7.9^c	61.3±7.0^c	71.0±7.5^c	82.5±7.4^c	118.1±9.8^c
过氧化氢含量 H₂O₂ content (μmol·g^-1FW)	CK	332.1±46.9^a	345.1±43.8^a	384.2±38.7^a	400.0±58.6^a	418.3±49.4^a	455.2±58.5^a
	A	301.4±55.4^a	472.1±46.7^b	454.1±52.2^b	521.2±50.8^b	490.9±75.8^b	697.4±68.6^b
	B	414.8±65.4^b	567.9±51.4^c	564.9±61.4^c	653.2±81.6^c	998.4±68.4^c	1040.6±75.8^c
	C	482.9±60.9^c	602.9±75.3^d	683.6±68.4^d	1018.3±77.5^d	1241.0±69.2^d	1244.3±65.3^d
丙二醛含量 MDAcontent (μmol·g^-1FW)	CK	79.6±11.7^a	89.4±10.9^a	87.4±9.7^a	96.2±14.7^a	100.9±12.4^a	124.9±14.6^a
	A	90.6±13.9^b	91.2±11.7^b	98.2±13.1^b	125.7±12.7^b	127.6±19.0^b	138.6±17.1^b
	B	106.9±16.4^c	108.9±12.9^c	120.6±15.3^c	162.9±20.4^c	190.5±17.1^c	202.9±18.9^c
	C	103.7±15.2^d	131.8±18.8^d	147.1±17.1^d	215.9±19.4^d	213.0±17.3^d	244.9±16.3^d