Chin J Plant Ecol ›› 2008, Vol. 32 ›› Issue (5): 1002-1006.DOI: 10.3773/j.issn.1005-264x.2008.05.004

• Original article • Previous Articles     Next Articles

INFLUENCE OF He-Ne LASER IRRADIATION ON PROTECTIVE ENZYME ACTIVITIES AND LIPID PEROXIDATION IN WHEAT SEEDLINGS BY DROUGHT STRESS DAMAGE

QIU Zong-Bo1,2, LIU Xiao1, LI Fang-Min1, TIAN Xiang-Jun1, YUE Ming1,*()   

  1. 1Key Laboratory of Resources Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi’an 710069, China
    2College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China
  • Received:2007-02-08 Accepted:2007-04-26 Online:2008-02-08 Published:2008-09-30
  • Contact: YUE Ming

Abstract:

Aims We studied protective enzyme activities and lipid peroxidation in wheat seedlings treated with different concentration of PEG6000 to determine the effects of He-Ne laser on wheat (Triticum aestivum)exposed to drought stress.

Method We treated 12-day-old, drought-stressed seedlings with 5%, 10% and 15% PEG6000 for 3, 6, 9, 12 and 15 days. After 3, 6, 9, 12 and 15 days of drought stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0, 1 and 3 min. Leaves were sampled for various analyses.

Important findings He-Ne laser irradiation significantly decreased malondialdehyde (MDA) content and superoxide radical (O2-.) production rate and notably increased peroxidase (POD) enzyme activity and ascorbate (AsA) and glutathione (GSH) concentration in wheat seedlings exposed to moderate drought stress. We concluded that 3 min laser irradiation enhanced anti-drought characteristics of wheat seedlings treated with 5% or 10% PEG6000.

Key words: He-Ne laser, wheat, drought stress, protective enzyme, lipid peroxidation